Abstract:
:We previously showed that the 93-bp region between the enhancer and promoter (named DEN for downstream of enhancer) of the long terminal repeat (LTR) of the MCF13 murine leukemia virus is an important determinant of the ability of this virus to induce thymic lymphoma. In this study we observed that DEN plays a role in the regulation of virus replication in the thymus during the preleukemic period. A NF-kappaB site in the DEN region partially contributes to the effect of DEN on both lymphomagenicity and virus replication. To further study the effects of DEN and the NF-kappaB site on viral pathogenicity during the preleukemic period, we examined replication of wild-type and mutant viruses with a deletion of the NF-kappaB site or the entire DEN region in the thymus. Thymic lymphocytes which were infected with wild-type and mutant viruses were predominantly the CD3(-) CD4(+) CD8(+) and CD3(+) CD4(+) CD8(+) cells. The increase in infection by wild-type virus and both mutant viruses of these two subpopulations during the preleukemic period ranged from 9- to 84-fold, depending upon the time point and virus. The major difference between the wild-type and both mutant viruses was the lower rate and lower level of mutant virus replication in these thymic subpopulations. Significant differences in replication between wild-type and both mutant viruses were seen in the CD3(-) CD4(+) CD8(+) and CD3(-) CD4(-) CD8(-) subpopulations, suggesting that these thymic cell types are important targets for viral transformation.
journal_name
J Viroljournal_title
Journal of virologyauthors
Yoshimura FK,Wang T,Cankovic Mdoi
10.1128/JVI.73.6.4890-4898.1999subject
Has Abstractpub_date
1999-06-01 00:00:00pages
4890-8issue
6eissn
0022-538Xissn
1098-5514journal_volume
73pub_type
杂志文章abstract::The immediate-early gene ie2 of murine cytomegalovirus was characterized. The 1.75-kb ie2 transcript is spliced from three exons, of 78, 124, and 1,283 nucleotides, which are separated by introns of 1,245 and 364 nucleotides. An ATG codon located in the third exon leads into an open reading frame of 391 codons. Immedi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.3.1638-1643.1991
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pub_type: 杂志文章
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doi:10.1128/JVI.38.3.1055-1063.1981
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pub_type: 杂志文章
doi:10.1128/JVI.68.2.988-1001.1994
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pub_type: 杂志文章
doi:10.1128/JVI.00364-11
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.1.4.738-746.1967
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pub_type: 杂志文章
doi:10.1128/JVI.61.10.3306-3309.1987
更新日期:1987-10-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/JVI.57.2.475-480.1986
更新日期:1986-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2015-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.6.4705-4712.1999
更新日期:1999-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jvi.76.12.6164-6171.2002
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journal_title:Journal of virology
pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/JVI.79.12.7777-7784.2005
更新日期:2005-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.52.3.1011-1012.1984
更新日期:1984-12-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/jvi.76.4.1959-1970.2002
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1977-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2009-12-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2015-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2018-10-12 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2019-12-12 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.5.1540-1545.1987
更新日期:1987-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03043-15
更新日期:2016-01-06 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2018-09-26 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.43.1.104-112.1982
更新日期:1982-07-01 00:00:00