Abstract:
:Monoclonal antibodies (MAbs) against the rubella virion were used to locate epitopes involved in hemagglutination and neutralization. The MAbs exhibiting high-level hemagglutination-inhibiting activity were shown by Western blot analysis to be specific for the E1 polypeptide; this is consistent with the presence of the hemagglutinin on the E1 polypeptide. Some of the E1-specific MAbs also neutralized viral infectivity. However, hemagglutination-inhibiting activity and neutralizing activity did not always correlate. Three distinct functional epitopes were identified on the E1 polypeptide by competition analyses: one which reacted with MAbs with high-level hemagglutination-inhibiting activity and with neutralizing activity, one which reacted with MAbs with low-level hemagglutination-inhibiting activity and with neutralizing activity, and one which reacted with MAbs with only hemagglutination-inhibiting activity. A MAb specific for the E2 polypeptide exhibited neutralizing activity. This E2-specific MAb and two E1-specific MAbs with neutralizing activity were capable of precipitating intact virus which indicates that at least three epitopes involved in neutralization are accessible on the surface of the virion.
journal_name
J Viroljournal_title
Journal of virologyauthors
Green KY,Dorsett PHdoi
10.1128/JVI.57.3.893-898.1986subject
Has Abstractpub_date
1986-03-01 00:00:00pages
893-8issue
3eissn
0022-538Xissn
1098-5514journal_volume
57pub_type
杂志文章abstract:UNLABELLED:Aspects of intrinsic antiviral immunity are mediated by promyelocytic leukemia nuclear body (PML-NB) constituent proteins. During herpesvirus infection, these antiviral proteins are independently recruited to nuclear domains that contain infecting viral genomes to cooperatively promote viral genome silencing...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00426-16
更新日期:2016-06-10 00:00:00
abstract::A phage, designated CP-51, that carries out generalized transduction in Bacillus cereus 569 was isolated from soil. All auxotrophic mutants tested, those requiring tryptophan, histidine, leucine, isoleucine, methionine, or phenylalanine, were transduced to prototrophy. The phage was extremely unstable when stored at 2...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.2.7.657-662.1968
更新日期:1968-07-01 00:00:00
abstract::We previously conducted a phase I/II study using arterial infusions of ONYX-015 (dl1520), a replication-selective adenoviral vector, with E1b deleted, for patients with metastatic colorectal cancer. No dose-limiting toxicities occurred, but >90% of the patients experienced fever. The effects of temperature on the repl...
journal_title:Journal of virology
pub_type: 临床试验,杂志文章
doi:10.1128/JVI.79.1.581-591.2005
更新日期:2005-01-01 00:00:00
abstract:UNLABELLED:The lipid kinase phosphatidylinositol 4-kinase III alpha (PI4KIIIα) is an endoplasmic reticulum (ER)-resident enzyme that synthesizes phosphatidylinositol 4-phosphate (PI4P). PI4KIIIα is an essential host factor for hepatitis C virus (HCV) replication. Interaction with HCV nonstructural protein 5A (NS5A) lea...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01063-14
更新日期:2014-09-01 00:00:00
abstract::Approximately 20 to 22 copies of adenovirus type 12 (Ad12) DNA per cell were integrated into the genome of the cell line T637. Only a few of these copies seemed to remain intact and colinear with virion DNA. All other persisting viral genomes exhibited deletions or inversions or both in the right-hand part of Ad12 DNA...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.42.1.317-321.1982
更新日期:1982-04-01 00:00:00
abstract::Interspecies transmission of the transmissible spongiform encephalopathies (TSEs), or prion diseases, can result in the adaptation and selection of TSE strains with an expanded host range and increased virulence such as in the case of bovine spongiform encephalopathy and variant Creutzfeldt-Jakob disease. To investiga...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.12.5542-5547.2000
更新日期:2000-06-01 00:00:00
abstract::The African swine fever virus protein A238L inhibits activation of NFAT transcription factor by binding calcineurin and inhibiting its phosphatase activity. NFAT controls the expression of many immunomodulatory proteins. Here we describe a 14-amino-acid region of A238L that is needed and sufficient for binding to calc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.20.9412-9420.2000
更新日期:2000-10-01 00:00:00
abstract::To study the variability of human immunodeficiency virus type 1 (HIV-1), we used immunotoxins to select for variants within a population of H9 cells persistently infected with a molecular clone of HIV-1 designated NL4-3. Chimeric immunotoxin CD4-PE40 (a chimeric fusion protein consisting of the amino-terminal two doma...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.1.75-81.1995
更新日期:1995-01-01 00:00:00
abstract::The parechoviruses differ in many biological properties from other picornaviruses, and their replication strategy is largely unknown. In order to identify the viral RNA replication complex in human parechovirus type 1 (HPEV-1)-infected cells, we located viral protein and RNA in correlation to virus-induced membrane al...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.15.8512-8523.2003
更新日期:2003-08-01 00:00:00
abstract::Purified Kunjin virus was disrupted with sodium dodecyl sulfate, urea, and mercaptoethanol or acetic acid. Electrophoresis on 7.5% polyacrylamide gel separated four proteins of high (120,000), intermediate (65,000) and low (18,000 and 13,000) molecular weights. A "core" particle was obtained by degradation of the viri...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.4.5.688-693.1969
更新日期:1969-11-01 00:00:00
abstract::Goat and rabbit antisera prepared against a purified Rauscher murine leukemia virus glycoprotein (gp69/71) rapidly neutralized spleen focus-forming virus in Rauscher and Friend virus preparations. Absorption studies revealed that most of the neutralizing activity of goat anti-Rauscher virus gp69/71 serum was directed ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.14.1.187-189.1974
更新日期:1974-07-01 00:00:00
abstract::The human immunodeficiency virus type 1 Tat protein binds to an RNA stem-loop structure called TAR which is present at the 5' end of all human immunodeficiency virus type 1 transcripts. This binding is centered on a bulge within the stem of TAR and is an essential step in the trans-activation process which results in ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.10.5196-5202.1991
更新日期:1991-10-01 00:00:00
abstract::Lymphocyte antigen 6E (LY6E) is a GPI-anchored, interferon-inducible protein that has been shown to modulate viral infection in a cell type-dependent manner. Our recent work showed that LY6E promotes HIV-1 infection in some high-CD4-expressing cells, including human peripheral blood mononuclear cells (PBMCs) and the S...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01866-18
更新日期:2019-03-21 00:00:00
abstract::Dengue virus (DENV) is a mosquito-borne virus that infects upward of 300 million people annually and has the potential to cause fatal hemorrhagic fever and shock. While the parameters contributing to dengue immunopathogenesis remain unclear, the collapse of redox homeostasis and the damage induced by oxidative stress ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01551-20
更新日期:2020-11-23 00:00:00
abstract::In order to analyze the function of VPg amplification in aphthoviruses, we have undertaken the first mutational analysis of the repetitive VPg-coding region using an improved foot-and-mouth disease virus (FMDV) cDNA clone from which infective viral RNA was synthesized. A set of VPg mutants was constructed by site-dire...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.4.2251-2260.1992
更新日期:1992-04-01 00:00:00
abstract::Herpes simplex virus 1 (HSV-1) causes significant morbidity and mortality in humans worldwide. HSV-1 enters epithelial cells via an endocytosis mechanism that is low-pH dependent. However, the precise intracellular pathway has not been identified, including the compartment where fusion occurs. In this study, we utiliz...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01910-20
更新日期:2020-11-23 00:00:00
abstract::In this study, we show that human cytomegalovirus DNA synthesis is inhibited in infected confluent human embryonic lung cells treated with the DNA-intercalative topoisomerase II inhibitor 4-9'-(acridinylamino)methanesulfon-m-anisidide (m-AMSA). Similar inhibitory effects were observed with VM-26, a nonintercalative to...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.12.4797-4800.1988
更新日期:1988-12-01 00:00:00
abstract::Bovine herpesvirus 1 (BHV-1) establishes a latent infection in sensory ganglionic neurons of cattle. During a latent infection, a single latency-related (LR) transcript is expressed. This observation suggested that DNA sequences in the LR promoter are positively regulated by neural cell type factors. The regulation of...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.10.6099-6106.1992
更新日期:1992-10-01 00:00:00
abstract::The lymphoid cell line #1670 has been derived from the infiltrated spleen of a tumor-bearing marmoset monkey infected with Herpesvirus saimiri. The cells contain both types of H. saimiri DNA, unique light (L-) DNA (36% cytosine plus guanine) and repetitive heavy (H-) DNA (71% cytosine plus guanine), without producing ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.22.3.794-803.1977
更新日期:1977-06-01 00:00:00
abstract::Retroviruses integrate into cellular DNA nonrandomly. Lentiviruses such as human immunodeficiency virus type 1 (HIV-1) favor the bodies of active genes and gene-enriched transcriptionally active regions of chromosomes. The interaction between lentiviral integrase and the cellular protein lens epithelium-derived growth...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01148-12
更新日期:2013-01-01 00:00:00
abstract::Human immunodeficiency virus (HIV) infects human cells by binding to surface CD4 molecules and directly fusing with the cell membrane. Although mouse cells expressing human CD4 bind HIV, they do not become infected, apparently because of a block in membrane fusion. To study this problem, we constructed a recombinant v...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.5.2149-2156.1990
更新日期:1990-05-01 00:00:00
abstract::GS-8374 is a potent HIV protease inhibitor (PI) with a unique diethyl-phosphonate moiety. Due to a balanced contribution of enthalpic and entropic components to its interaction with the protease (PR) active site, the compound retains activity against HIV mutants with high-level multi-PI resistance. We report here the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01211-12
更新日期:2013-01-01 00:00:00
abstract::The nucleotide sequence was determined for the hemagglutinin gene of the Hong Kong subtype influenza strain A/Bangkok/1/79. The amino acid sequence predicted from these data shows a total of 36 amino acid changes as compared with hemagglutinin for a 1968 Hong Kong strain, 11 more than had occurred in a 1975 strain. Th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.39.3.663-672.1981
更新日期:1981-09-01 00:00:00
abstract::Inoculation of the leafhopper cell line AC-20 with wound tumor virus resulted in a productive noncytopathic infection with no detectable alteration of cellular protein synthesis. Virus-specific polypeptide synthesis, detectable by 8 h postinoculation, increased in a linear fashion, reaching a peak (approximately 10 to...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.56.2.620-624.1985
更新日期:1985-11-01 00:00:00
abstract::In bacterial strains containing the deoxyribonuclease endonuclease I (endonuclease I(+) strains), 70 to 80% of the injected superinfecting T-even phage deoxyribonucleic acid (DNA) is rapidly degraded to oligonucleotides having an average chain length of 8, the same value as that obtained by endonuclease I digestion of...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.8.6.869-886.1971
更新日期:1971-12-01 00:00:00
abstract::The U(S)3 open reading frame of herpes simplex virus 1 (HSV-1) was reported to encode two mRNAs each directing the synthesis of the same protein. We report that the U(S)3 gene encodes two proteins. The predominant U(S)3 protein is made in wild-type HSV-1-infected cells. The truncated mRNA and a truncated protein desig...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.13.8470-8479.2005
更新日期:2005-07-01 00:00:00
abstract::The DNA sequence of 3,240 nucleotides of the XbaI G fragment located in the unique long (UL) region of the equine herpesvirus 1 genome revealed two major open reading frames (ORFs) designated UL3 and UL4. The UL3 ORF of 470 amino acids (aa) maps at nucleotides (nt) 4450 to 3038 from the long terminus, and its predicte...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.9.5363-5372.1992
更新日期:1992-09-01 00:00:00
abstract::Kaposi sarcoma-associated herpesvirus (KSHV) is necessary but not sufficient for primary effusion lymphoma (PEL) development. Alterations in cellular signaling pathways are also a characteristic of PEL. Other B cell lymphomas have acquired an oncogenic mutation in the myeloid differentiation primary response 88 (MYD88...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02123-19
更新日期:2020-05-04 00:00:00
abstract::Flock house virus is a small insect virus with a bipartite RNA genome consisting of RNA1 and RNA2. RNA3 is a subgenomic element encoded by RNA1, the genomic segment required for viral RNA synthesis (T. M. Gallagher, P. D. Friesen, and R. R. Rueckert, J. Virol. 46:481-489, 1983). Synthesis of RNA3 is strongly inhibited...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.5.2716-2722.1993
更新日期:1993-05-01 00:00:00
abstract::Avian leukosis virus subgroup J (ALV-J), an exogenous avian retrovirus, is thought to have evolved by recombination with the highly identical env gene of the endogenous avian retrovirus EAV-HP. Embryonic expression of EAV-HP env has been suggested to be associated with the induction of immunological tolerance, a featu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.23.11935-11938.2001
更新日期:2001-12-01 00:00:00