Abstract:
:Kaposi's sarcoma-associated herpesvirus (KSHV) (also called human herpesvirus 8) is consistently found in Kaposi's sarcoma lesions and in body-cavity-based lymphomas. A 17-kb KSHV lambda clone was obtained directly from a Kaposi's sarcoma lesion. DNA sequence analysis of this clone identified an open reading frame which has 32% amino acid identity and 53% similarity to the virus-encoded cyclin (v-cyclin) of herpesvirus saimiri (HVS) and 31% identity and 53% similarity to human cellular cyclin D2. This KSHV open reading frame was shown to encode a 29- to 30-kDa protein with the properties of a v-cyclin. KSHV v-cyclin protein was found to associate predominantly with cdk6, a cellular cyclin-dependent kinase known to interact with cellular type D cyclins and HVS v-cyclin. The KSHV v-cyclin was also found to associate weakly with cdk4. KSHV v-cyclin-cdk6 complexes strongly phosphorylated glutathione S-transferase-Rb fusion protein and histone H1 as substrates in vitro. Thus, KSHV v-cyclin resembles the v-cyclin of the T-lymphocyte-transforming HVS in its specificity for association with cdk6 and in its ability to strongly activate cdk6 protein kinase activity.
journal_name
J Viroljournal_title
Journal of virologyauthors
Li M,Lee H,Yoon DW,Albrecht JC,Fleckenstein B,Neipel F,Jung JUdoi
10.1128/JVI.71.3.1984-1991.1997subject
Has Abstractpub_date
1997-03-01 00:00:00pages
1984-91issue
3eissn
0022-538Xissn
1098-5514journal_volume
71pub_type
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更新日期:1970-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.7.4565-4571.1994
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.56.3.743-749.1985
更新日期:1985-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:2011-03-01 00:00:00
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