Analysis of the varicella-zoster virus IE62 N-terminal acidic transactivating domain and its interaction with the human mediator complex.

Abstract:

:The varicella-zoster virus major transactivator, IE62, contains a potent N-terminal acidic transcriptional activation domain (TAD). Our experiments revealed that the minimal IE62 TAD encompasses amino acids (aa) 19 to 67. We showed that the minimal TAD interacts with the human Mediator complex. Site-specific mutations revealed residues throughout the minimal TAD that are important for both activation and Mediator interaction. The TAD interacts directly with aa 402 to 590 of the MED25 subunit, and site-specific TAD mutations abolished this interaction. Two-dimensional nuclear magnetic resonance spectroscopy revealed that the TAD is intrinsically unstructured. Our studies suggest that transactivation may involve the TAD adopting a defined structure upon binding MED25.

journal_name

J Virol

journal_title

Journal of virology

authors

Yamamoto S,Eletsky A,Szyperski T,Hay J,Ruyechan WT

doi

10.1128/JVI.00054-09

subject

Has Abstract

pub_date

2009-06-01 00:00:00

pages

6300-5

issue

12

eissn

0022-538X

issn

1098-5514

pii

JVI.00054-09

journal_volume

83

pub_type

杂志文章
  • Functional characterization of heptad repeat 1 and 2 mutants of the spike protein of severe acute respiratory syndrome coronavirus.

    abstract::To understand the roles of heptad repeat 1(HR1) and HR2 of the spike (S) protein of the severe acute respiratory syndrome coronavirus (SARS-CoV) in virus-cell interactions, the conserved Leu or Ile residues located at positions 913, 927, 941, and 955 in HR1 and 1151, 1165, and 1179 in HR2 were individually replaced wi...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.80.7.3225-3237.2006

    authors: Chan WE,Chuang CK,Yeh SH,Chang MS,Chen SS

    更新日期:2006-04-01 00:00:00

  • Retrovirus purification: method that conserves envelope glycoprotein and maximizes infectivity.

    abstract::A Sepharose 4B chromatographic method for purification of retroviruses is described which was less time consuming, increased purified virus yields, conserved viral glycoprotein, and increased recovery of biological infectivity in comparison with conventional sucrose gradient ultracentrifugation techniques. ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.25.3.923-927.1978

    authors: McGrath M,Witte O,Pincus T,Weissman IL

    更新日期:1978-03-01 00:00:00

  • Spatial localization of the Ebola virus glycoprotein mucin-like domain determined by cryo-electron tomography.

    abstract::The Ebola virus glycoprotein mucin-like domain (MLD) is implicated in Ebola virus cell entry and immune evasion. Using cryo-electron tomography of Ebola virus-like particles, we determined a three-dimensional structure for the full-length glycoprotein in a near-native state and compared it to that of a glycoprotein la...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.00870-14

    authors: Tran EE,Simmons JA,Bartesaghi A,Shoemaker CJ,Nelson E,White JM,Subramaniam S

    更新日期:2014-09-01 00:00:00

  • Human endogenous retrovirus K (HML-2) elements in the plasma of people with lymphoma and breast cancer.

    abstract::Actively replicating endogenous retroviruses entered the human genome millions of years ago and became a stable part of the inherited genetic material. They subsequently acquired multiple mutations, leading to the assumption that these viruses no longer replicate. However, certain human tumor cell lines have been show...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.00646-08

    authors: Contreras-Galindo R,Kaplan MH,Leissner P,Verjat T,Ferlenghi I,Bagnoli F,Giusti F,Dosik MH,Hayes DF,Gitlin SD,Markovitz DM

    更新日期:2008-10-01 00:00:00

  • The Road Less Traveled: HIV's Use of Alternative Routes through Cellular Pathways.

    abstract::Pathogens such as HIV-1, with their minimalist genomes, must navigate cellular networks and rely on hijacking and manipulating the host machinery for successful replication. Limited overlap of host factors identified as vital for pathogen replication may be explained by considering that pathogens target, rather than s...

    journal_title:Journal of virology

    pub_type: 杂志文章,评审

    doi:10.1128/JVI.03684-14

    authors: Marx A,Alian A

    更新日期:2015-05-01 00:00:00

  • Structure-based mutational analysis of the C-terminal DNA-binding domain of human immunodeficiency virus type 1 integrase: critical residues for protein oligomerization and DNA binding.

    abstract::The C-terminal domain of human immunodeficiency virus type 1 (HIV-1) integrase (IN) is a dimer that binds to DNA in a nonspecific manner. The structure of the minimal region required for DNA binding (IN220-270) has been solved by nuclear magnetic resonance spectroscopy. The overall fold of the C-terminal domain of HIV...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.72.6.4841-4848.1998

    authors: Lutzke RA,Plasterk RH

    更新日期:1998-06-01 00:00:00

  • Epstein-Barr virus LMP2A alters in vivo and in vitro models of B-cell anergy, but not deletion, in response to autoantigen.

    abstract::A significant percentage of the population latently harbors Epstein-Barr virus (EBV) in B cells. One EBV-encoded protein, latent membrane protein 2A (LMP2A), is expressed in tissue culture models of EBV latent infection, in human infections, and in many of the EBV-associated proliferative disorders. LMP2A constitutive...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.79.12.7355-7362.2005

    authors: Swanson-Mungerson MA,Caldwell RG,Bultema R,Longnecker R

    更新日期:2005-06-01 00:00:00

  • Within-Host Variations of Human Papillomavirus Reveal APOBEC Signature Mutagenesis in the Viral Genome.

    abstract::Persistent infection with oncogenic human papillomaviruses (HPVs) causes cervical cancer, accompanied by the accumulation of somatic mutations into the host genome. There are concomitant genetic changes in the HPV genome during viral infection; however, their relevance to cervical carcinogenesis is poorly understood. ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.00017-18

    authors: Hirose Y,Onuki M,Tenjimbayashi Y,Mori S,Ishii Y,Takeuchi T,Tasaka N,Satoh T,Morisada T,Iwata T,Miyamoto S,Matsumoto K,Sekizawa A,Kukimoto I

    更新日期:2018-05-29 00:00:00

  • Defects in virion production caused by mutations affecting the C-terminal portion of the Moloney murine leukemia virus capsid protein.

    abstract::The capsid (CA) domain of the Moloney murine leukemia virus (Mo-MuLV) Gag protein has a unique carboxy terminus with a highly charged arginine-rich sequence. Mutant viruses harboring arginine-to-alanine mutations affecting this region of CA displayed significant defects in virion release, and the few viral particles p...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/jvi.77.5.3339-3344.2003

    authors: Wang MQ,Goff SP

    更新日期:2003-03-01 00:00:00

  • Mechanisms of human papillomavirus E2-mediated repression of viral oncogene expression and cervical cancer cell growth inhibition.

    abstract::The papillomavirus E2 gene product plays a pivotal role in viral replication. E2 has multiple functions, including (i) transcriptional activation and repression of viral promoters and (ii) the enhancement of viral DNA replication. It was previously reported that E2 suppressed the growth of papillomavirus-positive cerv...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/jvi.74.8.3752-3760.2000

    authors: Nishimura A,Ono T,Ishimoto A,Dowhanick JJ,Frizzell MA,Howley PM,Sakai H

    更新日期:2000-04-01 00:00:00

  • Proteolytic processing of the Sindbis virus membrane protein precursor PE2 is nonessential for growth in vertebrate cells but is required for efficient growth in invertebrate cells.

    abstract::We have shown previously that processing of the Sindbis virus envelope protein precursor PE2 to envelope protein E2 is not required for virus maturation in cultured vertebrate fibroblast cells and that unprocessed PE2 can be incorporated into infectious virus in place of E2 (J. F. Presley and D. T. Brown, J. Virol. 63...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.65.4.1905-1909.1991

    authors: Presley JF,Polo JM,Johnston RE,Brown DT

    更新日期:1991-04-01 00:00:00

  • Noncytopathic bovine viral diarrhea virus inhibits double-stranded RNA-induced apoptosis and interferon synthesis.

    abstract::Bovine viral diarrhea virus (BVDV), a pestivirus of the Flaviviridae family, is an economically important cattle pathogen with a worldwide distribution. Both noncytopathic (ncp) and cytopathic (cp) biotypes of BVDV can be isolated from persistently infected cattle suffering from the lethal mucosal disease. The cp biot...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.75.10.4692-4698.2001

    authors: Schweizer M,Peterhans E

    更新日期:2001-05-01 00:00:00

  • Functional analysis of the complex trans-activating response element RNA structure in simian immunodeficiency virus.

    abstract::Transcription of human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) is activated through binding of the viral Tat protein to the trans-activating response (TAR) element at the 5' end of the nascent transcript. Whereas HIV type 1 (HIV-1) TAR folds a simple hairpin structure, the corresponding do...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.00530-08

    authors: Centlivre M,Klaver B,Berkhout B,Das AT

    更新日期:2008-09-01 00:00:00

  • Isolation and characterization of human papillomavirus type 6-specific T cells infiltrating genital warts.

    abstract::The potential role of T cells in the control of human papillomavirus type 6 (HPV-6) infections is an appealing premise, but their actual role has been sparsely investigated. Since HPV-6 infections are confined to the epithelium, such an investigation should focus on the T cells present at the site of infection (i.e., ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.71.9.6427-6432.1997

    authors: Hong K,Greer CE,Ketter N,Van Nest G,Paliard X

    更新日期:1997-09-01 00:00:00

  • Middle East respiratory syndrome coronavirus accessory protein 4a is a type I interferon antagonist.

    abstract::Middle East respiratory syndrome coronavirus (MERS-CoV) causes severe acute respiratory infection with as yet unclear epidemiology. We previously showed that MERS-CoV counteracts parts of the innate immune response in human bronchiolar cells. Here we analyzed accessory proteins 3, 4a, 4b, and 5 for their abilities to ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.01845-13

    authors: Niemeyer D,Zillinger T,Muth D,Zielecki F,Horvath G,Suliman T,Barchet W,Weber F,Drosten C,Müller MA

    更新日期:2013-11-01 00:00:00

  • Structural requirements for trans activation of human immunodeficiency virus type 1 long terminal repeat-directed gene expression by tat: importance of base pairing, loop sequence, and bulges in the tat-responsive sequence.

    abstract::In order to elucidate the molecular mechanisms of action of the tat-responsive sequence, mutational analysis of the tat-responsive sequence was carried out. The most critical region comprised nucleotides +18 to +44 and included the 3-nucleotide bulge at positions +23 to +25, the loop sequence, and an intact stem. In a...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.64.3.1402-1406.1990

    authors: Roy S,Parkin NT,Rosen C,Itovitch J,Sonenberg N

    更新日期:1990-03-01 00:00:00

  • Resistance mutations in human immunodeficiency virus type 1 integrase selected with elvitegravir confer reduced susceptibility to a wide range of integrase inhibitors.

    abstract::Integration of viral DNA into the host chromosome is an essential step in the life cycle of retroviruses and is facilitated by the viral integrase enzyme. The first generation of integrase inhibitors recently approved or currently in late-stage clinical trials shows great promise for the treatment of human immunodefic...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.00470-08

    authors: Goethals O,Clayton R,Van Ginderen M,Vereycken I,Wagemans E,Geluykens P,Dockx K,Strijbos R,Smits V,Vos A,Meersseman G,Jochmans D,Vermeire K,Schols D,Hallenberger S,Hertogs K

    更新日期:2008-11-01 00:00:00

  • Use of zonal ultracentrifuge systems for biophysical studies of rhinoviruses.

    abstract::This paper reports the use of zonal ultracentrifuge techniques to conduct biophysical studies of rhinoviruses grown with WI-38 cells. Good clean-out of infectivity from rhinovirus harvests was obtained with the continuous-flow B-V and B-IX rotors. Use of the B-V rotor resulted in the successful concentration of rhinov...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.2.9.937-943.1968

    authors: Gerin JL,Richter WR,Fenters JD,Holper JC

    更新日期:1968-09-01 00:00:00

  • Murine leukemia virus uses NXF1 for nuclear export of spliced and unspliced viral transcripts.

    abstract:UNLABELLED:Intron-containing mRNAs are subject to restricted nuclear export in higher eukaryotes. Retroviral replication requires the nucleocytoplasmic transport of both spliced and unspliced RNA transcripts, and RNA export mechanisms of gammaretroviruses are poorly characterized. Here, we report the involvement of the...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.03584-13

    authors: Sakuma T,Davila JI,Malcolm JA,Kocher JP,Tonne JM,Ikeda Y

    更新日期:2014-04-01 00:00:00

  • Defective assembly of influenza A virus due to a mutation in the polymerase subunit PA.

    abstract::The RNA-dependent RNA polymerase of influenza A virus is composed of three subunits that together synthesize all viral mRNAs and also replicate the viral genomic RNA segments (vRNAs) through intermediates known as cRNAs. Here we describe functional characterization of 16 site-directed mutants of one polymerase subunit...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.80.1.252-261.2006

    authors: Regan JF,Liang Y,Parslow TG

    更新日期:2006-01-01 00:00:00

  • Phenotypic and genetic analysis of Lymantria dispar nucleopolyhedrovirus few polyhedra mutants: mutations in the 25K FP gene may be caused by DNA replication errors.

    abstract::We previously demonstrated that polyhedron formation (PF) mutants arise at a high frequency during serial passage of the Lymantria dispar nucleopolyhedrovirus (LdMNPV) in the L. dispar 652Y cell line (J. M. Slavicek, N. Hayes-Plazolles, and M. E. Kelly, Biol. Control 5:251-261, 1995). Most of these PF mutants exhibite...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.71.2.1097-1106.1997

    authors: Bischoff DS,Slavicek JM

    更新日期:1997-02-01 00:00:00

  • Localization of the single-stranded RNA-binding domains of bluetongue virus nonstructural protein NS2.

    abstract::The S2 gene of bluetongue virus, serotype 17, has been cloned, and the nonstructural protein NS2 has been expressed. Synthetic peptides matching regions within the amino acid sequence of NS2 were used to map three single-stranded RNA (ssRNA)-binding regions within the protein. A prokaryotic expression system was then ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/jvi.76.2.499-506.2002

    authors: Fillmore GC,Lin H,Li JK

    更新日期:2002-01-01 00:00:00

  • Susceptibility of AKXD recombinant inbred mouse strains to lymphomas.

    abstract::We analyzed the susceptibility of 10 AKXD recombinant inbred (RI) mouse strains to lymphomas. These strains were derived from crosses of AKR/J, a highly lymphomatous strain, and DBA/2J, a weakly lymphomatous strain. Of the 10 strains analyzed, nine showed a high incidence of lymphoma development. As with the other 13 ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.67.4.2083-2090.1993

    authors: Gilbert DJ,Neumann PE,Taylor BA,Jenkins NA,Copeland NG

    更新日期:1993-04-01 00:00:00

  • Increased immunogenicity of human immunodeficiency virus gp120 engineered to express Galalpha1-3Galbeta1-4GlcNAc-R epitopes.

    abstract::The glycan shield comprised of multiple carbohydrate chains on the human immunodeficiency virus (HIV) envelope glycoprotein gp120 helps the virus to evade neutralizing antibodies. The present study describes a novel method for increasing immunogenicity of gp120 vaccine by enzymatic replacement of sialic acid on these ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.00310-06

    authors: Abdel-Motal U,Wang S,Lu S,Wigglesworth K,Galili U

    更新日期:2006-07-01 00:00:00

  • Alterations in the structure of the oligosaccharide of vesicular stomatitis virus G protein by swainsonine.

    abstract::Swainsonine, an inhibitor of glycoprotein processing, inhibits the formation of the normal oligosaccharide chain of the G protein of vesicular stomatitis virus. Thus, when vesicular stomatitis virus was grown in baby hamster kidney cells in the presence of swainsonine (15 to 500 ng/ml) and labeled with [2-(3)H]mannose...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.46.1.60-69.1983

    authors: Kang MS,Elbein AD

    更新日期:1983-04-01 00:00:00

  • Viruses and RNA interference: issues and controversies.

    abstract::The question of whether any mammalian cells are able to mount an effective RNA interference-mediated antiviral innate immune response has remained highly controversial. In this Gem, I review recent data addressing this important issue and propose a testable hypothesis that can explain many of the apparently contradict...

    journal_title:Journal of virology

    pub_type: 杂志文章,评审

    doi:10.1128/JVI.01179-14

    authors: Cullen BR

    更新日期:2014-11-01 00:00:00

  • Immunodominance in virus-induced CD8(+) T-cell responses is dramatically modified by DNA immunization and is regulated by gamma interferon.

    abstract::The phenomenon whereby the host immune system responds to only a few of the many possible epitopes in a foreign protein is termed immunodominance. Immunodominance occurs not only during microbial infection but also following vaccination, and clarification of the underlying mechanism may permit the rational design of v...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/jvi.76.9.4251-4259.2002

    authors: Rodriguez F,Harkins S,Slifka MK,Whitton JL

    更新日期:2002-05-01 00:00:00

  • Kaposi's Sarcoma-Associated Herpesvirus Viral Interferon Regulatory Factor 4 (vIRF4) Perturbs the G1-S Cell Cycle Progression via Deregulation of the cyclin D1 Gene.

    abstract::Kaposi's sarcoma-associated herpesvirus (KSHV) infection modulates the host cell cycle to create an environment optimal for its viral-DNA replication during the lytic life cycle. We report here that KSHV vIRF4 targets the β-catenin/CBP cofactor and blocks its occupancy on the cyclin D1 promoter, suppressing the G1-S c...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.01897-15

    authors: Lee HR,Mitra J,Lee S,Gao SJ,Oh TK,Kim MH,Ha T,Jung JU

    更新日期:2015-10-21 00:00:00

  • Mapping a neutralizing epitope onto the capsid of adeno-associated virus serotype 8.

    abstract::Adeno-associated viruses (AAVs) are small single-stranded DNA viruses that can package and deliver nongenomic DNA for therapeutic gene delivery. AAV8, a liver-tropic vector, has shown great promise for the treatment of hemophilia A and B. However, as with other AAV vectors, host anti-capsid immune responses are a dete...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.00218-12

    authors: Gurda BL,Raupp C,Popa-Wagner R,Naumer M,Olson NH,Ng R,McKenna R,Baker TS,Kleinschmidt JA,Agbandje-McKenna M

    更新日期:2012-08-01 00:00:00

  • Differences in cytokine and chemokine responses during neurological disease induced by polytropic murine retroviruses Map to separate regions of the viral envelope gene.

    abstract::Infection of the central nervous system (CNS) by several viruses can lead to upregulation of proinflammatory cytokines and chemokines. In immunocompetent adults, these molecules induce prominent inflammatory infiltrates. However, with immunosuppressive retroviruses, such as human immunodeficiency virus (HIV), little C...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.75.6.2848-2856.2001

    authors: Peterson KE,Robertson SJ,Portis JL,Chesebro B

    更新日期:2001-03-01 00:00:00