Structural determinant of human La protein critical for internal initiation of translation of hepatitis C virus RNA.

abstract：:Although recombinant human adenovirus (HAV)-based vectors offer several advantages for somatic gene therapy and vaccination over other viral vectors, it would be desirable to develop alternative vectors with prolonged expression and decreased toxicity. Toward this objective, a replication-defective bovine adenovirus t...

journal_title：Journal of virology

authors： Reddy PS,Idamakanti N,Chen Y,Whale T,Babiuk LA,Mehtali M,Tikoo SK

更新日期：1999-11-01 00:00:00

abstract：:A malignant glioma cell line was infected with the human T-lymphotropic virus type IIIB isolate of the human immunodeficiency virus. Infection appeared to be latent rather than productive. Through contact with monocytic or lymphoid cells, the virus present in the glioma cells could be transmitted and gave rise to a fu...

journal_title：Journal of virology

authors： Chiodi F,Fuerstenberg S,Gidlund M,Asjö B,Fenyö EM

更新日期：1987-04-01 00:00:00

abstract：:The 3C region of human rhinovirus 14 was expressed in Escherichia coli. The microbially synthesized protease was functional, since the expressed precursor underwent autoproteolytic processing to generate mature molecules of the expected molecular weight and antigenicity. Mutation of the putative active-site Cys-146 re...

journal_title：Journal of virology

authors： Cordingley MG,Register RB,Callahan PL,Garsky VM,Colonno RJ

更新日期：1989-12-01 00:00:00

abstract：:Proteasome inhibitors (e.g., bortezomib, MG132) are known to enhance adeno-associated virus (AAV) transduction; however, whether this results from pleotropic proteasome inhibition or off-target serine and/or cysteine protease inhibition remains unresolved. Here, we examined recombinant AAV (rAAV) effects of a new prot...

journal_title：Journal of virology

authors： Mitchell AM,Samulski RJ

更新日期：2013-12-01 00:00:00

abstract：:Three defense functions of immunoglobulin A (IgA), immune exclusion, intracellular neutralization, and virus excretion, were assessed in a measles virus model using polarized epithelial cells expressing the polymeric immunoglobulin receptor and monoclonal antibodies against the viral H and F envelope proteins and the ...

journal_title：Journal of virology

authors： Yan H,Lamm ME,Björling E,Huang YT

更新日期：2002-11-01 00:00:00

abstract：:Lymphocyte antigen 6E (LY6E) is a GPI-anchored, interferon-inducible protein that has been shown to modulate viral infection in a cell type-dependent manner. Our recent work showed that LY6E promotes HIV-1 infection in some high-CD4-expressing cells, including human peripheral blood mononuclear cells (PBMCs) and the S...

journal_title：Journal of virology

authors： Yu J,Liang C,Liu SL

更新日期：2019-03-21 00:00:00

abstract：:In Escherichia coli B infected with phage T4 with mutations in gene 55 the shut-off of synthesis of two early enzymes was found to be delayed 10 to 15 min as compared to wild-type T4 infection. The kinetics of early enzyme formation was very similar to that obtained after DNA-negative mutant infection. The transcripti...

journal_title：Journal of virology

authors： Bolund C

更新日期：1973-07-01 00:00:00

abstract：:Koi herpesvirus (KHV) is the causative agent of a lethal disease in koi and common carp. In the present study, we describe the cloning of the KHV genome as a stable and infectious bacterial artificial chromosome (BAC) clone that can be used to produce KHV recombinant strains. This goal was achieved by the insertion of...

journal_title：Journal of virology

authors： Costes B,Fournier G,Michel B,Delforge C,Raj VS,Dewals B,Gillet L,Drion P,Body A,Schynts F,Lieffrig F,Vanderplasschen A

更新日期：2008-05-01 00:00:00

abstract：:TNPO3 is a nuclear importer required for HIV-1 infection. Here, we show that depletion of TNPO3 leads to an HIV-1 block after nuclear import but prior to integration. To investigate the mechanistic requirement of TNPO3 in HIV-1 infection, we tested the binding of TNPO3 to the HIV-1 core and found that TNPO3 binds to t...

journal_title：Journal of virology

authors： Valle-Casuso JC,Di Nunzio F,Yang Y,Reszka N,Lienlaf M,Arhel N,Perez P,Brass AL,Diaz-Griffero F

更新日期：2012-05-01 00:00:00

abstract：:We have designed a ribozyme that cleaves human immunodeficiency virus type 1 (HIV-1) RNA in U5 (at nucleotide +115). This ribozyme was tested in vitro and was found to give efficient and specific digestion of RNA containing the HIV-1 U5 sequence. When the U5 ribozyme was placed into the HIV-1 genome, virus replication...

journal_title：Journal of virology

authors： Dropulić B,Lin NH,Martin MA,Jeang KT

更新日期：1992-03-01 00:00:00

abstract：:It has been shown previously in the severe acute respiratory syndrome coronavirus (SARS-CoV) that two point mutations, N15A and V25F, in the transmembrane domain (TMD) of the envelope (E) protein abolished channel activity and led to in vivo attenuation. Pathogenicity was recovered in mutants that also regained E prot...

journal_title：Journal of virology

authors： To J,Surya W,Fung TS,Li Y,Verdià-Bàguena C,Queralt-Martin M,Aguilella VM,Liu DX,Torres J

更新日期：2017-02-14 00:00:00

abstract：:We have generated four mouse monoclonal antibodies (MAbs) to bovine papillomavirus virions that bound type-specific, adjacent, and conformationally dependent epitopes on the L1 major capsid protein. All four MAbs were neutralizing at ratios of 1 MAb molecule per 5 to 25 L1 molecules, but only three effectively blocked...

journal_title：Journal of virology

authors： Roden RB,Weissinger EM,Henderson DW,Booy F,Kirnbauer R,Mushinski JF,Lowy DR,Schiller JT

更新日期：1994-11-01 00:00:00

abstract：:Autonomous parvoviruses are thought to uniquely encapsidate single-stranded DNA of minus polarity. In contrast, the defective adeno-associated viruses separately encapsidate equal amounts of plus and minus DNA strands. We reexamined the uniqueness of minus strand encapsidation for the autonomous parvoviruses. Although...

journal_title：Journal of virology

authors： Bates RC,Snyder CE,Banerjee PT,Mitra S

更新日期：1984-02-01 00:00:00

abstract：:Circulating interferon production, induced by Newcastle disease virus, is about seven times higher in C(57) Black mice than i Balb/c/Gif mice. A Mendelian analysis was carried out and circulating interferon production was measured in reciprocal F(1) hybrids, in the F(2) generation, in progeny of backcrosses of F(1) hy...

journal_title：Journal of virology

authors： de Maeyer E,de Maeyer-Guignard J

更新日期：1969-05-01 00:00:00

abstract：:Myxoma virus (MV) encodes a cell surface protein (M135R) that is predicted to mimic the host alpha/beta interferon receptor (IFN-alpha/beta-R) and thus prevent IFN-alpha/beta from triggering a host antiviral response. This prediction is based on sequence similarity to B18R, the viral IFN-alpha/beta-R from vaccinia vir...

journal_title：Journal of virology

authors： Barrett JW,Sypula J,Wang F,Alston LR,Shao Z,Gao X,Irvine TS,McFadden G

更新日期：2007-01-01 00:00:00

abstract：:Genetic and phylogenetic analyses suggest that the pandemic H1N1/2009 virus was derived from well-established swine influenza lineages; however, there is no convincing evidence that the pandemic virus was generated from a direct precursor in pigs. Furthermore, the evolutionary dynamics of influenza virus in pigs have ...

journal_title：Journal of virology

authors： Wei K,Sun H,Sun Z,Sun Y,Kong W,Pu J,Ma G,Yin Y,Yang H,Guo X,Chang KC,Liu J

更新日期：2014-10-01 00:00:00

abstract：:In an attempt to generate broadly cross-reactive, neutralizing monoclonal antibodies (MAbs) to simian immunodeficiency virus (SIV), we compared two immunization protocols using different preparations of oligomeric SIV envelope (Env) glycoproteins. In the first protocol, mice were immunized with soluble gp140 (sgp140) ...

journal_title：Journal of virology

authors： Edinger AL,Ahuja M,Sung T,Baxter KC,Haggarty B,Doms RW,Hoxie JA

更新日期：2000-09-01 00:00:00

abstract：:The essential components of the immune system that control primary and chronic infection with herpes simplex virus type 1 (HSV-1) in mice were investigated. Infection within the first few days can be controlled by alpha/beta interferon (IFN-alpha/beta) alone without significant contribution of B, T, or NK cells. IFN-a...

journal_title：Journal of virology

authors： Vollstedt S,Arnold S,Schwerdel C,Franchini M,Alber G,Di Santo JP,Ackermann M,Suter M

更新日期：2004-04-01 00:00:00

abstract：:Deletion mutants of bacteriphage T5 can be identified by analyzing the DNA content of single plaques, after alkaline denaturation, by agarose gel electrophoresis. Use of this procedure has resulted in the isolation of a new mutant, designated del2, that lacks a 1.6% segment located 68 to 70% from the left end of T5 DN...

journal_title：Journal of virology

authors： Rhoades M,Schwartz J,Wahl JM

更新日期：1980-11-01 00:00:00

abstract：:N6-adenosine methylation (m6A) is the most common posttranscriptional RNA modification in mammalian cells. We found that most transcripts encoded by the Kaposi's sarcoma-associated herpesvirus (KSHV) genome undergo m6A modification. The levels of m6A-modified mRNAs increased substantially upon stimulation for lytic re...

journal_title：Journal of virology

authors： Ye F,Chen ER,Nilsen TW

更新日期：2017-07-27 00:00:00

abstract：:The ectopic overexpression of Bcl-2 restricts both influenza A virus-induced apoptosis and influenza A virus replication in MDCK cells, thus suggesting a role for Bcl-2 family members during infection. Here we report that influenza A virus cannot establish an apoptotic response without functional Bax, a downstream tar...

journal_title：Journal of virology

authors： McLean JE,Datan E,Matassov D,Zakeri ZF

更新日期：2009-08-01 00:00:00

abstract：:The 5'-untranslated regions (5' UTRs) of picornavirus genomes contain an internal ribosomal entry site (IRES) that promotes the end-independent initiation of translation. Picornavirus IRESs are classified into four structurally distinct groups, each with different initiation factor requirements. Here, we identify a fi...

journal_title：Journal of virology

authors： Sweeney TR,Dhote V,Yu Y,Hellen CU

更新日期：2012-02-01 00:00:00

abstract：:Epstein-Barr virus (EBV) can be reactivated from latency into the lytic cycle by many stimuli believed to operate by different mechanisms. Cell lines containing EBV differ in their responses to inducing stimuli, yet all stimuli require de novo protein synthesis (44). A crucial step preliminary to identifying these pro...

journal_title：Journal of virology

authors： Countryman J,Gradoville L,Bhaduri-McIntosh S,Ye J,Heston L,Himmelfarb S,Shedd D,Miller G

更新日期：2009-10-01 00:00:00

abstract：:The E3 complex transcription unit of adenovirus encodes four major mRNAs (a, c, f, and h) and two minor (d and e) mRNAs with overlapping exons, alternative splice sites, and two polyadenylation sites, termed E3A (upstream) and E3B (downstream). mRNAs a and d use the E3A polyadenylation site, and mRNAs c, e, f, and h u...

journal_title：Journal of virology

authors： Brady HA,Scaria A,Wold WS

更新日期：1992-10-01 00:00:00

abstract：:Human immunodeficiency virus type 1 (HIV-1) requires that its genome be reverse transcribed into double-stranded DNA for productive infection of cells. This process requires not only reverse transcriptase but also the nucleocapsid protein (NC), which functions as a nucleic acid chaperone. Reverse transcription general...

journal_title：Journal of virology

authors： Thomas JA,Bosche WJ,Shatzer TL,Johnson DG,Gorelick RJ

更新日期：2008-10-01 00:00:00

abstract：:Nef is a regulatory gene product of human immunodeficiency virus type 1 (HIV-1) and other primate lentiviruses which enhances virion infectivity by an unknown mechanism. We report here that Nef is detectable at moderate levels in preparations of HIV-1 virions which lack active viral protease (PR). Significantly smalle...

journal_title：Journal of virology

authors： Bukovsky AA,Dorfman T,Weimann A,Göttlinger HG

更新日期：1997-02-01 00:00:00

abstract：:The inhibition of protein synthesis by cycloheximide markedly stimulated adenovirus 2 early transcription (assayed in vivo and in vitro in nuclei) when the drug was added 3 h after infection of HeLa cells. The stimulation was not uniform but ranged from three- to eightfold for region 1A to 10- to 30-fold for region 2 ...

journal_title：Journal of virology

authors： Cross FR,Darnell JE Jr

更新日期：1983-02-01 00:00:00

abstract：:Closed circular unintegrated DNA of the SEATO strain of gibbon ape leukemia virus (GaLV-S) was isolated from canine thymus fibroblasts after cocultivation with chronically infected bat lung fibroblasts. Restriction endonuclease HindIII cleaves GaLV-S DNA once, thus allowing isolation and cloning of HindIII-digested un...

journal_title：Journal of virology

authors： Gelmann EP,Trainor CD,Wong-Staal F,Reitz MS

更新日期：1982-10-01 00:00:00

abstract：:Retrovirus assembly involves a complex series of events in which a large number of proteins must be targeted to a point on the plasma membrane where immature viruses bud from the cell. Gag polyproteins of most retroviruses assemble an immature capsid on the cytoplasmic side of the plasma membrane during the budding pr...

journal_title：Journal of virology

authors： Parker SD,Hunter E

更新日期：2000-01-01 00:00:00

abstract：:The mouse hind footpad inoculation model has served as a standard laboratory system for the study of the neuropathogenesis of herpes simplex virus type 1 (HSV-1) infection. The temporal and spatial distribution of viral antigen, known as the transneuronal spread phenotype, has not previously been described; nor is it ...

journal_title：Journal of virology

authors： Engel JP,Madigan TC,Peterson GM

更新日期：1997-03-01 00:00:00