Abstract:
:We have designed a ribozyme that cleaves human immunodeficiency virus type 1 (HIV-1) RNA in U5 (at nucleotide +115). This ribozyme was tested in vitro and was found to give efficient and specific digestion of RNA containing the HIV-1 U5 sequence. When the U5 ribozyme was placed into the HIV-1 genome, virus replication was suppressed in tissue culture. Introduction of this ribozyme into cells by using an amphotropic retrovirus vector significantly reduced expression of U5-containing RNA in cells chronically infected with HIV-1. Naive T cells were cocultivated with packaging cells that produce defective amphotropic retroviruses containing the U5 ribozyme. These lymphocytes were found to be partially protected from HIV-1 infection.
journal_name
J Viroljournal_title
Journal of virologyauthors
Dropulić B,Lin NH,Martin MA,Jeang KTdoi
10.1128/JVI.66.3.1432-1441.1992subject
Has Abstractpub_date
1992-03-01 00:00:00pages
1432-41issue
3eissn
0022-538Xissn
1098-5514journal_volume
66pub_type
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