Abstract:
:Human papillomaviruses (HPVs) infect squamous epithelium and establish their genomes as episomes in proliferating basal cells. As infected cells differentiate, the viral DNA is amplified to high copy number and infectious virus is produced. Viral production has not yet been observed in vitro due to the inability of standard culture methods to duplicate most stages of epithelial differentiation. In this study, we have examined a cell line derived from a low-grade cervical lesion and found that it contained episomal copies of an HPV-31 subtype, HPV-31b, at approximately 50 copies per cell. When allowed to stratify at the air-liquid interface of in vitro raft cultures, this cell line differentiates in a manner which histologically resembles a low-grade cervical lesion in vivo. We have observed the amplification of HPV-31b genomes in distinct foci in the upper portion of the in vitro-stratified epithelium similar to that found in productive HPV infections in vivo. Although transcripts from the late region of HPV-31b were also detected specifically in stratified raft cultures, no capsid protein was found. These studies duplicate one important aspect of a productive HPV infection in vitro, the differentiation-dependent amplification of papillomavirus genomes.
journal_name
J Viroljournal_title
Journal of virologyauthors
Bedell MA,Hudson JB,Golub TR,Turyk ME,Hosken M,Wilbanks GD,Laimins LAdoi
10.1128/JVI.65.5.2254-2260.1991subject
Has Abstractpub_date
1991-05-01 00:00:00pages
2254-60issue
5eissn
0022-538Xissn
1098-5514journal_volume
65pub_type
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journal_title:Journal of virology
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.7.3530-3537.1991
更新日期:1991-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.68.4.2347-2354.1994
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.7.3.352-358.1971
更新日期:1971-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.46.1.196-203.1983
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pub_type: 杂志文章
doi:10.1128/JVI.68.5.3298-3307.1994
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pub_type: 杂志文章
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更新日期:1991-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2014-01-01 00:00:00
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journal_title:Journal of virology
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