Abstract:
:Adeno-associated virus type 2 (AAV2) preferentially integrates its genome into the AAVS1 locus on human chromosome 19. Preferential integration requires the AAV2 Rep68 or Rep78 protein (Rep68/78), a Rep68/78 binding site (RBS), and a nicking site within AAVS1 and may also require an RBS within the virus genome. To obtain further information that might help to elucidate the mechanism and preferred substrate configurations of preferential integration, we amplified junctions between AAV2 DNA and AAVS1 from AAV2-infected HeLaJW cells and cells with defective Artemis or xeroderma pigmentosum group A genes. We sequenced 61 distinct junctions. The integration junction sequences show the three classical types of nonhomologous-end-joining joints: microhomology at junctions (57%), insertion of sequences that are not normally contiguous with either the AAV2 or the AAVS1 sequences at the junction (31%), and direct joining (11%). These junctions were spread over 750 bases and were all downstream of the Rep68/78 nicking site within AAVS1. Two-thirds of the junctions map to 350 bases of AAVS1 that are rich in polypyrimidine tracts on the nicked strand. The majority of AAV2 breakpoints were within the inverted terminal repeat (ITR) sequences, which contain RBSs. We never detected a complete ITR at a junction. Residual ITRs at junctions never contained more than one RBS, suggesting that the hairpin form, rather than the linear ITR, is the more frequent integration substrate. Our data are consistent with a model in which a cellular protein other than Artemis cleaves AAV2 hairpins to produce free ends for integration.
journal_name
J Viroljournal_title
Journal of virologyauthors
McAlister VJ,Owens RAdoi
10.1128/JVI.00746-07subject
Has Abstractpub_date
2007-09-01 00:00:00pages
9718-26issue
18eissn
0022-538Xissn
1098-5514pii
JVI.00746-07journal_volume
81pub_type
杂志文章abstract::Three fish retroviruses infecting walleyes constitute the recently recognized genus called epsilonretrovirus. The founding member of this group, walleye dermal sarcoma virus (WDSV), induces benign skin tumors in the infected fish and replicates near 4 degrees C. While the viral genomic sequence is known, biochemical c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.9.4341-4349.2002
更新日期:2002-05-01 00:00:00
abstract::We previously conducted a phase I/II study using arterial infusions of ONYX-015 (dl1520), a replication-selective adenoviral vector, with E1b deleted, for patients with metastatic colorectal cancer. No dose-limiting toxicities occurred, but >90% of the patients experienced fever. The effects of temperature on the repl...
journal_title:Journal of virology
pub_type: 临床试验,杂志文章
doi:10.1128/JVI.79.1.581-591.2005
更新日期:2005-01-01 00:00:00
abstract::Reoviruses are encapsidated double-stranded RNA viruses that cause systemic disease in mice after peroral (p.o.) inoculation and primary replication in the intestine. In this study, we define components of the immune system involved in the clearing of reovirus from the proximal small intestine. The intestines of immun...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.2.1109-1116.1996
更新日期:1996-02-01 00:00:00
abstract::The herpes simplex virus type 1 (HSV-1) UL25 gene contains a 580-amino-acid open reading frame that codes for an essential protein. Previous studies have shown that the UL25 gene product is a virion component (M. A. Ali et al., Virology 216:278-283, 1996) involved in virus penetration and capsid assembly (C. Addison e...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.2.1060-1070.1998
更新日期:1998-02-01 00:00:00
abstract::Broadly neutralizing antibodies have been isolated that bind the glycan shield of the HIV-1 envelope spike. One such antibody, PGT135, contacts the intrinsic mannose patch of gp120 at the Asn332, Asn392, and Asn386 glycosylation sites. Here, site-specific glycosylation analysis of recombinant gp120 revealed glycan mic...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00230-15
更新日期:2015-07-01 00:00:00
abstract::SER virus, a paramyxovirus that is closely related to simian virus 5 (SV5), is unusual in that it fails to induce syncytium formation. The SER virus F protein has an unusually long cytoplasmic tail (CT), and it was previously observed that truncations or specific mutations of this domain result in enhanced syncytium f...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.16.8513-8523.2004
更新日期:2004-08-01 00:00:00
abstract::The folded genome of Escherichia coli is converted to a slower-sedimenting form within 5 min after infection with bacteriophage T4 or T4nd28(den A)-amN82(44). Chloramphenicol sensitivity and response to UV-irradiation of the phage suggest participation of viral-induced functions. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.2.548-550.1974
更新日期:1974-02-01 00:00:00
abstract::The envelope protein E of flaviviruses mediates both receptor-binding and membrane fusion. At the virion surface, 180 copies of E are tightly packed and organized in a herringbone-like icosahedral structure, whereas in noninfectious subviral particles, 60 copies are arranged in a T=1 icosahedral symmetry. In both case...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00660-09
更新日期:2009-09-01 00:00:00
abstract::Epstein-Barr virus (EBV) infection leads to lifelong viral persistence through its latency in B cells. EBV-specific T cells control reactivations and prevent the development of EBV-associated malignancies in most healthy carriers, but infection can sometimes cause chronic disease and malignant transformation. Epstein-...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00852-13
更新日期:2013-08-01 00:00:00
abstract::Natural killer (NK) cells derived from the human female reproductive tract (FRT) are phenotypically and functionally distinct from those obtained from peripheral blood. Because the FRT is a primary site of human immunodeficiency virus type 1 (HIV-1) infection in women, we determined whether soluble factors secreted by...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00562-09
更新日期:2009-11-01 00:00:00
abstract::The Sindbis virus minimal subgenomic mRNA promoter (spanning positions -19 to +5 relative to the subgenomic mRNA start site) is approximately three- to sixfold less active than the fully active -98 to +14 promoter region. We identified two elements flanking the -19 to +5 region which increase its transcription to leve...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.8.3509-3519.2001
更新日期:2001-04-01 00:00:00
abstract:UNLABELLED:Major histocompatibility complex class II (MHC-II) molecules play a central role in adaptive antiviral immunity by presenting viral peptides to CD4(+) T cells. Due to their key role in adaptive immunity, many viruses, including Kaposi's sarcoma-associated herpesvirus (KSHV), have evolved multiple strategies ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03713-14
更新日期:2015-05-01 00:00:00
abstract::Normal replication of Moloney murine leukemia virus (MoMLV) requires the integration of a DNA copy of the viral RNA genome into a chromosome of the host. In this work, we characterize the DNA sequences at the ends of the linear proviral precursor that are required for integration in the presence of MoMLV integration p...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.11.5645-5648.1990
更新日期:1990-11-01 00:00:00
abstract::Herpesviruses encode an essential, maturational serine protease whose catalytic domain, assemblin (28 kDa), is released by self-cleavage from a 74-kDa precursor (pPR, pUL80a). Although there is considerable information about the structure and enzymatic characteristics of assemblin, a potential pharmacologic target, co...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02821-06
更新日期:2007-04-01 00:00:00
abstract::The proteolytic enzyme, thermolysin, degraded the external segment of the membrane glycoprotein of intact vesicular stomatitis (VS) virions but left behind a small nonglycosylated fragment, presumably embedded in the virion membrane. Other proteases generated membrane-associated glycoprotein fragments differing somewh...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.16.2.237-240.1975
更新日期:1975-08-01 00:00:00
abstract::A gene encoding a homolog of glycoprotein B of herpes simplex virus (gB homolog) has been identified on the Marek's disease virus (MDV) genome (L. J. N. Ross, M. Sanderson, S. D. Scott, M. M. Binns, T. Doel, and B. Milne, J. Gen. Virol. 70:1789-1804, 1989); however, the molecular and immunological characteristics of t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.5.2631-2638.1992
更新日期:1992-05-01 00:00:00
abstract::The spikes of alphaviruses are composed of three copies of an E2-E1 heterodimer. The E1 protein possesses membrane fusion activity, and the E2 protein, or its precursor form, p62 (sometimes called PE2), controls this function. Both proteins are, together with the viral capsid protein, translated from a common C-p62-E1...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.12.9654-9663.1997
更新日期:1997-12-01 00:00:00
abstract::Retroviruses are believed to induce tumors by acting as insertional mutagens that activate expression of cellular protooncogenes. Indeed, almost 90% of mouse mammary tumor virus (MMTV)-induced mammary tumors in C3H/He mice show upregulation of Int protooncogenes. We have analyzed three different MMTV variants [MMTV(C3...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.19.8876-8883.2000
更新日期:2000-10-01 00:00:00
abstract::It has been suggested that sequences located within the 5' noncoding region of human foamy virus (HFV) are critical for expression of the viral Gag and Pol structural proteins. Here, we identify a discrete approximately 151-nucleotide sequence, located within the R region of the HFV long terminal repeat, that activate...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.15.6817-6824.2001
更新日期:2001-08-01 00:00:00
abstract::Cloned avian leukosis viral DNAs were mutagenized in the long terminal repeat, in the leader sequence for env mRNA, and at the poly-env junction. The effect of these mutations in the viral DNA upon its ability to direct virus production or env mRNA synthesis was analyzed by microinjecting the mutant DNAs into chicken ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.43.2.503-510.1982
更新日期:1982-08-01 00:00:00
abstract::The tempo and intensity of retroviral neuropathogenesis are dependent on the capacity of the virus to invade the central nervous system. For murine leukemia viruses, an important determinant of neuroinvasiveness is the virus-encoded protein glycosylated Gag, the function of which in the virus life cycle is not known. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.7.5619-5625.1998
更新日期:1998-07-01 00:00:00
abstract:UNLABELLED:Human immunodeficiency virus (HIV) seizes control of cellular cullin-RING E3 ubiquitin ligases (CRLs) to promote viral replication. HIV-1 Vpr and HIV-2/simian immunodeficiency virus (SIV) Vpr and Vpx engage the cullin4 (CUL4)-containing ubiquitin ligase complex (CRL4) to cause polyubiquitination and proteaso...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00241-14
更新日期:2014-06-01 00:00:00
abstract::Type I interferons induce a complex transcriptional program that leads to a generalized antiviral response against a large panel of viruses, including human immunodeficiency virus type 1 (HIV-1). However, despite the fact that interferons negatively regulate HIV-1 ex vivo, a chronic interferon state is linked to the p...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02553-12
更新日期:2013-03-01 00:00:00
abstract::A uniform nomenclature has been agreed upon for monoclonal antibodies directed against virus-coded proteins of simian virus 40 and polyoma virus. Blocks of numbers from PAb1 to PAb999 have been allocated to workers involved in the isolation of monoclonal antibodies of this type. The correspondence between PAb numbers ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.41.2.709-709.1982
更新日期:1982-02-01 00:00:00
abstract::The retroviral Gag protein plays the central role in the assembly process and can form membrane-enclosed, virus-like particles in the absence of any other viral products. These particles are similar to authentic virions in density and size. Three small domains of the human immunodeficiency virus type 1 (HIV-1) Gag pro...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.6.4667-4677.1998
更新日期:1998-06-01 00:00:00
abstract::Among prion infections, two scenarios of prion spread are generally observed: (i) early lymphoid tissue replication or (ii) direct neuroinvasion without substantial antecedent lymphoid amplification. In nature, cervids are infected with chronic wasting disease (CWD) prions by oral and nasal mucosal exposure, and studi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00077-17
更新日期:2017-04-28 00:00:00
abstract::The antigenic binding sites of two monoclonal antibodies are located in the COOH-terminal region (clone 412) and probably in an internal region (clone 7) of simian virus 40 large T antigen. A third monoclonal antibody (clone 122), which has been shown to bind nonviral T antigen, does not react with HeLa cells infected...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.37.1.478-482.1981
更新日期:1981-01-01 00:00:00
abstract::A bacteriophage with a contractile tail which shows very similar features to R-type pyocins was isolated and characterized. This phage, named PS17,was purified by DEAE-cellulose chromatography and CsCl density gradient centrifugation. It was a DNA-containing phage, and the density of the purified particles in CsCl was...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.32.3.951-957.1979
更新日期:1979-12-01 00:00:00
abstract::Linear hepadnavirus DNA in primary hepatocyte cultures efficiently participates in intra- and intermolecular nonhomologous recombination at its ends. The products of this recombination are (i) monomeric covalently closed circular DNAs (cccDNAs) with deletions and insertions around the site of joining and (ii) oligomer...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.7.4029-4036.1995
更新日期:1995-07-01 00:00:00
abstract::We report here the isolation by molecular cloning and the analysis by heteroduplex and restriction enzyme mapping of seven distinct DNA fragments containing hepatitis B virus (HBV) sequences from genomic DNA of the PLC/PRF/5 human liver carcinoma cell line (the Alexander cell). No intact full-length HBV genomes were p...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.51.3.776-787.1984
更新日期:1984-09-01 00:00:00