Abstract:
:Second-site revertants from replication-incompetent molecular clones of human immunodeficiency virus (HIV) contain base substitutions adjacent to the TATA motif. The altered TATA box motifs were analyzed for their effect(s) on virus infectivity, long terminal repeat (LTR)-directed expression in transient transfection assays, in vitro RNA synthesis, and assembly of the TFIID-TFIIA preinitiation complex. The revertant TATA boxes accelerated the kinetics of HIV replication when present in the context of an LTR containing a Sp1 mutation (deletion or site specific); no effect was observed on the infectivity of wild-type HIV. In chloramphenicol acetyltransferase assays and in vitro transcription systems, the altered TATA box motifs led to elevated basal levels of RNA synthesis from NF-kappa B- and Sp1-mutagenized and wild-type templates, respectively, but did not increase responsiveness to Tat transactivation. The revertant TATA boxes accelerated the binding of TFIID and TFIIA to the LTR and stabilized their association with the promoter. The revertants did not assemble a more-processive elongation complex. These results suggest that in the context of an impaired enhancer/promoter (viz., three mutated Sp1 elements), a series of HIV revertants emerge which contain LTR alterations that significantly augment basal RNA synthesis. The TATA motif revertants are capable of rescuing the enhancer/promoter defect and sustain virus infectivity.
journal_name
J Viroljournal_title
Journal of virologyauthors
Kashanchi F,Shibata R,Ross EK,Brady JN,Martin MAdoi
10.1128/JVI.68.5.3298-3307.1994subject
Has Abstractpub_date
1994-05-01 00:00:00pages
3298-307issue
5eissn
0022-538Xissn
1098-5514journal_volume
68pub_type
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/JVI.39.3.945-949.1981
更新日期:1981-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.70.9.6502-6507.1996
更新日期:1996-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章,评审
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更新日期:2010-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02925-14
更新日期:2015-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.11.5.655-664.1973
更新日期:1973-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02155-18
更新日期:2019-04-03 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.7.3413-3417.2000
更新日期:2000-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.6.4705-4712.1999
更新日期:1999-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:2015-11-18 00:00:00
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pub_type: 杂志文章
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更新日期:2003-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2018-06-13 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2015-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1973-11-01 00:00:00
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更新日期:1993-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1988-10-01 00:00:00