Abstract:
:A fibrosarcoma was induced in a hamster by bovine papilloma virus type 2 (BPV2). The content of BPV2 DNA sequences was measured by DNA-DNA and cRNA-DNA hybridizations. The tumor contained approximately 300 BPV2 genome equivalents per cell. Southern blot hybridization indicated that the viral DNA was in free form, the entire genome most likely being present. In situ hybridization with BPV2 cRNA showed that multiple genome copies were present in each cell. Neither virus particles nor virus coat antigens could be detected in the tumor. A cell line was established from the fibrosarcoma, and the cells contained multiple copies of the BPV2 genome. The latter was in free form, and all of the DNA sequences appeared to be present in multiple copies and in all cells. An extensive search failed to reveal the presence of virus or viral antigens.
journal_name
J Viroljournal_title
Journal of virologyauthors
Moar MH,Campo MS,Laird HM,Jarrett WFdoi
10.1128/JVI.39.3.945-949.1981subject
Has Abstractpub_date
1981-09-01 00:00:00pages
945-9issue
3eissn
0022-538Xissn
1098-5514journal_volume
39pub_type
杂志文章abstract::Hantaviruses are tripartite negative-sense RNA viruses and members of the Bunyaviridae family. The nucleocapsid (N) protein is encoded by the smallest of the three genome segments (S). N protein is the principal structural component of the viral capsid and is central to the hantavirus replication cycle. We examined in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.15.8281-8288.2004
更新日期:2004-08-01 00:00:00
abstract::Infection of cells with enveloped viruses is accomplished through membrane fusion. The binding and fusion processes are mediated by the spike proteins in the envelope of the virus particle and usually involve a series of conformational changes in these proteins. We have studied the low-pH-mediated fusion process of th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.12.7309-7318.1992
更新日期:1992-12-01 00:00:00
abstract::A human cell line (293) transformed by adenovirus type 5 encodes mRNA's and proteins from the early region 1 (E1) of the viral genome. These products correspond to those synthesized early after adenovirus infection of normal cells. This pattern of expression is different from that observed at later times in the lytic ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.36.3.860-871.1980
更新日期:1980-12-01 00:00:00
abstract::During lytic infection, herpes simplex virus subverts the host cell RNA polymerase II transcription machinery to efficiently express its own genome while repressing the expression of most cellular genes. The mechanism by which RNA polymerase II is directed to the viral delayed-early and late genes is still unresolved....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.2.988-1001.1994
更新日期:1994-02-01 00:00:00
abstract::The glycan shield comprised of multiple carbohydrate chains on the human immunodeficiency virus (HIV) envelope glycoprotein gp120 helps the virus to evade neutralizing antibodies. The present study describes a novel method for increasing immunogenicity of gp120 vaccine by enzymatic replacement of sialic acid on these ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00310-06
更新日期:2006-07-01 00:00:00
abstract::Herpes simplex virus type 1 (HSV-1) is a DNA virus that acquires an envelope by budding into the inner nuclear membrane of an infected cell. Recombinant HSV-1 lacking the U(L)34 gene cannot undergo this event. U(L)34 and U(L)31, another viral protein, colocalize in an infected cell and are necessary and sufficient to ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.13.7601-7610.2003
更新日期:2003-07-01 00:00:00
abstract::Complexes of f2 phage RNA and its A protein, or maturation protein, transfect Escherichia coli cells much better than does protein-free RNA. We used these complexes to introduce the bacteriophage f2 lysis gene into cells. The A protein-RNA complex was found to kill cells, probably by causing them to leak large macromo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.39.1.60-66.1981
更新日期:1981-07-01 00:00:00
abstract::The VP7 and VP4 genes of seven antigenic mutants of simian rotavirus SA11 4fM (serotype 3) selected after 39 passages in the presence of SA11 4fM hyperimmune antiserum, were sequenced. Nucleotide sequence analysis indicated the following. (i) Twice as many amino acid substitutions occurred in the VP7 protein than in V...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.9.4534-4539.1990
更新日期:1990-09-01 00:00:00
abstract::Phosphorylation of viral proteins plays important roles in the influenza A virus (IAV) life cycle. By using mass spectrometry, we identified tyrosine 132 (Y132) as a phosphorylation site of the matrix protein (M1) of the influenza virus A/WSN/1933(H1N1). Phosphorylation at this site is essential to the process of viru...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03024-12
更新日期:2013-06-01 00:00:00
abstract::The initial aim of this study was to combine attributes of adeno-associated virus (AAV) and adenovirus (Ad) gene therapy vectors to generate an Ad-AAV hybrid vector allowing efficient site-specific integration with Ad vectors. In executing our experimental strategy, we found that, in addition to the known incompatibil...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.11.5411-5421.2002
更新日期:2002-06-01 00:00:00
abstract::The final assembly of rotavirus particles takes place in the endoplasmic reticulum (ER). In this work, we evaluated by RNA interference the relevance to rotavirus assembly and infectivity of grp78, protein disulfide isomerase (PDI), grp94, calnexin, calreticulin, and ERp57, members of the two ER folding systems descri...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02751-07
更新日期:2008-06-01 00:00:00
abstract::The effect DNA repair might have on the integration of exogenous proviral DNA into host cell DNA was investigated by comparing the efficiency of proviral DNA integration in normal chicken embryonic fibroblasts and in chicken embryonic fibroblasts treated with UV or 4-nitroquinoline-1-oxide. The cells were treated with...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.23.3.533-542.1977
更新日期:1977-09-01 00:00:00
abstract::Hepatitis C virus (HCV), a member of the family Flaviviridae, is a leading cause of chronic liver disease and cancer. Recent advances in HCV therapeutics have resulted in improved cure rates, but an HCV vaccine is not available and is urgently needed to control the global pandemic. Vaccine development has been hampere...
journal_title:Journal of virology
pub_type: 杂志文章,评审
doi:10.1128/JVI.00874-14
更新日期:2014-09-01 00:00:00
abstract::Mammalian cells infected with herpes simplex virus (HSV) express a novel ribonucleotide reductase which is biochemically and immunologically distinct from the uninfected-cell enzyme. Using polyvalent rabbit antiserum raised against partially purified HSV type 2 reductase as well as monoclonal antibodies to HSV type 1 ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.49.2.591-593.1984
更新日期:1984-02-01 00:00:00
abstract::Immediate-early viral gene products of human cytomegalovirus (HCMV) are derived from several genomic loci and largely serve to establish a cellular environment conducive to viral replication. We have further examined an unusual immediate-early transcript known as the 5-kb RNA, concluding that it is a stable intron enc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.23.13182-13189.2004
更新日期:2004-12-01 00:00:00
abstract::Large numbers of polymorphonuclear leukocytes (PMNs) infiltrated the murine vaginal mucosa within 24 h after intravaginal inoculation with an attenuated strain of herpes simplex virus type 2 (HSV-2). The role of these cells in resolution of a primary genital infection and in protection of HSV-immune animals against ch...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.8.6380-6386.1999
更新日期:1999-08-01 00:00:00
abstract::Rotavirus NSP5 is a nonstructural protein that localizes in cytoplasmic viroplasms of infected cells. NSP5 interacts with NSP2 and undergoes a complex posttranslational hyperphosphorylation, generating species with reduced polyacrylamide gel electrophoresis mobility. This process has been suggested to be due in part t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.7.3461-3470.2002
更新日期:2002-04-01 00:00:00
abstract::The effect of monoclonal antibodies on the growth of herpes simplex virus type 1 in trigeminal ganglia was investigated. Four-week-old mice were infected on an abrased cornea with herpes simplex virus type 1. Forty-eight hours after infection, trigeminal ganglia ipsilateral with infected eyes were removed and placed i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.51.3.656-661.1984
更新日期:1984-09-01 00:00:00
abstract::We investigated the features of the Moloney murine sarcoma virus leader sequence necessary for RNA packaging function by using a deletion analysis approach. We found that sequences that extend beyond those characterized genetically in previous reports are important for optimal packaging efficiency. A fragment covering...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.9.4643-4648.2002
更新日期:2002-05-01 00:00:00
abstract::The Ebola filoviruses are aggressive pathogens that cause severe and often lethal hemorrhagic fever syndromes in humans and nonhuman primates. To date, no effective therapies have been identified. To analyze the entry and fusion properties of Ebola virus, we adapted a human immunodeficiency virus type 1 (HIV-1) virion...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.2.918-926.2005
更新日期:2005-01-01 00:00:00
abstract::In vitro transcriptase activity of three group I temperature-sensitive (ts) mutants of vesicular stomatitis virus restricted at 39 C was restored by L-protein fractions derived from wild-type (wt) vesicular stomatitis virion nucleo-capsids. Soluble NS protein from wt nucleocapsids did not reconstitute restricted trans...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.18.2.596-603.1976
更新日期:1976-05-01 00:00:00
abstract::Anti-VPg immunoprecipitable RNA labeled in vitro during a poliovirus RNA polymerase reaction was formed by the elongation of VPg-containing template fragments rather than by initiation with VPg. The reaction was dependent on a host factor (terminal uridylyl transferase). The incorporation of labeled UTP could be detec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.58.1.212-215.1986
更新日期:1986-04-01 00:00:00
abstract::The cellular transcription factors Sp1 and NF-kappaB were upregulated shortly after the binding of purified live or UV-inactivated human cytomegalovirus (HCMV) to the cell surface. The rapid time frame of transcription factor induction is similar to that seen in other systems in which cellular factors are induced foll...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.7.5051-5059.1997
更新日期:1997-07-01 00:00:00
abstract::It has been previously shown that rotavirus maturation and stability of the outer capsid are calcium-dependent processes. More recently, it has been hypothesized that penetration of the cell membrane is also affected by conformational changes of the capsid induced by Ca2+. In this study, we determined quantitatively t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.8.4877-4883.1996
更新日期:1996-08-01 00:00:00
abstract::The route of internalization of the pestivirus bovine viral diarrhea virus (BVDV) was studied by using different chemical and biophysical inhibitors of endocytosis. Expression of the dominant-negative mutant Dyn(K44A) of the GTPase dynamin in MDBK cells, as well as the treatment of the cells with chlorpromazine and be...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.7.4191-4200.2005
更新日期:2005-04-01 00:00:00
abstract::We previously reported that herpes simplex virus type 1 (HSV-1) can activate the stress-activated protein kinases (SAPKs) p38 and JNK. In the present study, we undertook a comprehensive and comparative analysis of the requirements for viral protein synthesis in the activation of JNK and p38. Infection with the UL36 mu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.13.8348-8360.2005
更新日期:2005-07-01 00:00:00
abstract::Measles virus (MeV), like all viruses of the order Mononegavirales, utilizes a complex consisting of genomic RNA, nucleoprotein, the RNA-dependent RNA polymerase, and a polymerase cofactor, the phosphoprotein (P), for transcription and replication. We previously showed that a recombinant MeV that does not express anot...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01733-19
更新日期:2020-01-31 00:00:00
abstract::The severe acute respiratory syndrome coronavirus (SARS-CoV) was recently identified as the etiology of SARS. The virus particle consists of four structural proteins: spike (S), small envelope (E), membrane (M), and nucleocapsid (N). Recognition of a specific sequence, termed the packaging signal (PS), by a virus N pr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.22.13848-13855.2005
更新日期:2005-11-01 00:00:00
abstract::The inactivation of type C retroviruses by human serum may be a considerable impediment to the use of retroviral vectors in vivo for gene therapy. Here we show that virus inactivation is dependent both on the virus and on the cell line used to produce the virus. All viruses produced from murine NIH 3T3 or dog Cf2ThS+L...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.12.8001-8007.1994
更新日期:1994-12-01 00:00:00
abstract::Following infection by pairs of reovirus isolates consisting of combinations of reovirus T1 Lang, T2 Jones, or T3 Dearing, we found that one of the isolates interfered with the yield of progeny RNA derived from the other parents. The most significant interference was produced by T2 Jones or T3 Dearing, when mixed with...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.10.6667-6671.1994
更新日期:1994-10-01 00:00:00