Abstract:
:Rotavirus NSP5 is a nonstructural protein that localizes in cytoplasmic viroplasms of infected cells. NSP5 interacts with NSP2 and undergoes a complex posttranslational hyperphosphorylation, generating species with reduced polyacrylamide gel electrophoresis mobility. This process has been suggested to be due in part to autophosphorylation. We developed an in vitro phosphorylation assay using as a substrate an in vitro-translated NSP5 deletion mutant that was phosphorylated by extracts from MA104 cells transfected with NSP5 mutants but not by extracts from mock-transfected cells. The phosphorylated products obtained showed shifts in mobility similar to what occurs in vivo. From these and other experiments we concluded that NSP5 activates a cellular kinase(s) for its own phosphorylation. Three NSP5 regions were found to be essential for kinase(s) activation. Glutathione S-transferase-NSP5 mutants were produced in Escherichia coli and used to determine phosphoacceptor sites. These were mapped to four serines (Ser(153), Ser(155), Ser(163), and Ser(165)) within an acidic region with homology to casein kinase II (CKII) phosphorylation sites. CKII was able to phosphorylate NSP5 in vitro. NSP5 and its mutants fused to enhanced green fluorescent protein were used in transfection experiments followed by virus infection and allowed the determination of the domains essential for viroplasm localization in the context of virus infection.
journal_name
J Viroljournal_title
Journal of virologyauthors
Eichwald C,Vascotto F,Fabbretti E,Burrone ORdoi
10.1128/jvi.76.7.3461-3470.2002subject
Has Abstractpub_date
2002-04-01 00:00:00pages
3461-70issue
7eissn
0022-538Xissn
1098-5514journal_volume
76pub_type
杂志文章abstract::Enterovirus 71 (EV71) (genus Enterovirus, family Picornaviridae), a common cause of hand, foot, and mouth disease (HFMD), may also cause severe neurological diseases, such as encephalitis and poliomyelitis-like paralysis. To examine the genetic diversity and rate of evolution of EV71, we have determined and analyzed c...
journal_title:Journal of virology
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doi:10.1128/JVI.73.12.9969-9975.1999
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abstract:UNLABELLED:Hepatitis B virus (HBV)-specific cytotoxic T lymphocytes (CTLs) are critical in eliminating infection. We developed an animal model in which HBV-infected human hepatocytes are targeted by HBV-specific CTLs. After HBV inoculation in human hepatocyte-transplanted herpes simplex virus type-1 thymidine kinase-NO...
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.20.13082-13093.2005
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.10.3914-3917.1988
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.55.3.890-891.1985
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pub_type: 杂志文章
doi:10.1128/JVI.79.24.15388-15397.2005
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01514-06
更新日期:2007-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.23.14606-14613.2005
更新日期:2005-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.4.2508-2514.1995
更新日期:1995-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.5.2922-2927.1993
更新日期:1993-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01345-10
更新日期:2010-12-01 00:00:00
abstract::The product of the human cytomegalovirus (HCMV) UL71 gene is conserved throughout the herpesvirus family. During HCMV infection, protein pUL71 is required for efficient virion egress and is involved in the final steps of secondary envelopment leading to infectious viral particles. We found strong indications for oligo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06556-11
更新日期:2012-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.2.661-671.2001
更新日期:2001-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.58.2.468-474.1986
更新日期:1986-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.75.3.1476-1486.2001
更新日期:2001-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.6.2921-2934.2004
更新日期:2004-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.2.1250-1254.1994
更新日期:1994-02-01 00:00:00
abstract::Human cytomegalovirus (HCMV) has emerged as a clinically opportunistic pathogen that targets multiple types of ocular cells and tissues, including the iris region of the uveal tract during anterior uveitis. In this report, we used primary cultures of human iris stroma (HIS) cells derived from human eye donors to inves...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00109-15
更新日期:2015-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00524-17
更新日期:2017-08-24 00:00:00
abstract::To determine mechanisms by which persistent viral infections are established and maintained, we initiated persistent infections of murine erythroleukemia (MEL) cells by using reovirus strains type 3 Abney and type 3 Dearing. Establishment of persistent reovirus infections of MEL cells was not associated with a signifi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.1.299-306.1997
更新日期:1997-01-01 00:00:00
abstract::Human CD81 has been previously identified as the putative receptor for the hepatitis C virus envelope glycoprotein E2. The large extracellular loop (LEL) of human CD81 differs in four amino acid residues from that of the African green monkey (AGM), which does not bind E2. We mutated each of the four positions in human...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.8.3642-3649.2000
更新日期:2000-04-01 00:00:00
abstract::Two different proteins with high affinities for the pteridine ring of folic acid have been used to determine the location of this portion of the folate molecule in the tail plate of T4D and other T-even bacteriophage particles. The two proteins used were (i) antibody specific for folic acid and (ii) the folate-binding...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.16.6.1391-1400.1975
更新日期:1975-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.4.962-971.1987
更新日期:1987-04-01 00:00:00
abstract:UNLABELLED:The potency and breadth of the recently isolated neutralizing human monoclonal antibodies to HIV-1 have stimulated interest in their use to prevent or to treat HIV-1 infection. Due to the antigenically diverse nature of the HIV-1 envelope (Env), no single antibody is highly active against all viral strains. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02097-15
更新日期:2015-12-01 00:00:00
abstract::A single phosphorylation event at T-antigen residue Thr124 regulates initiation of simian virus 40 DNA replication. To explore this regulatory process, a series of peptides were synthesized, centered on Thr124. These peptides contain a nuclear localization signal (NLS) and a recognition site for cyclin/Cdk kinases. Wh...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.23.11785-11792.2002
更新日期:2002-12-01 00:00:00