Abstract:
UNLABELLED:Paramyxoviruses and other negative-strand RNA viruses encode matrix proteins that coordinate the virus assembly process. The matrix proteins link the viral glycoproteins and the viral ribonucleoproteins at virus assembly sites and often recruit host machinery that facilitates the budding process. Using a co-affinity purification strategy, we have identified the beta subunit of the AP-3 adapter protein complex, AP3B1, as a binding partner for the M proteins of the zoonotic paramyxoviruses Nipah virus and Hendra virus. Binding function was localized to the serine-rich and acidic Hinge domain of AP3B1, and a 29-amino-acid Hinge-derived polypeptide was sufficient for M protein binding in coimmunoprecipitation assays. Virus-like particle (VLP) production assays were used to assess the relationship between AP3B1 binding and M protein function. We found that for both Nipah virus and Hendra virus, M protein expression in the absence of any other viral proteins led to the efficient production of VLPs in transfected cells, and this VLP production was potently inhibited upon overexpression of short M-binding polypeptides derived from the Hinge region of AP3B1. Both human and bat (Pteropus alecto) AP3B1-derived polypeptides were highly effective at inhibiting the production of VLPs. VLP production was also impaired through small interfering RNA (siRNA)-mediated depletion of AP3B1 from cells. These findings suggest that AP-3-directed trafficking processes are important for henipavirus particle production and identify a new host protein-virus protein binding interface that could become a useful target in future efforts to develop small molecule inhibitors to combat paramyxoviral infections. IMPORTANCE:Henipaviruses cause deadly infections in humans, with a mortality rate of about 40%. Hendra virus outbreaks in Australia, all involving horses and some involving transmission to humans, have been a continuing problem. Nipah virus caused a large outbreak in Malaysia in 1998, killing 109 people, and smaller outbreaks have since occurred in Bangladesh and India. In this study, we have defined, for the first time, host factors that interact with henipavirus M proteins and contribute to viral particle assembly. We have also defined a new host protein-viral protein binding interface that can potentially be targeted for the inhibition of paramyxovirus infections.
journal_name
J Viroljournal_title
Journal of virologyauthors
Sun W,McCrory TS,Khaw WY,Petzing S,Myers T,Schmitt APdoi
10.1128/JVI.02103-14subject
Has Abstractpub_date
2014-11-01 00:00:00pages
13099-110issue
22eissn
0022-538Xissn
1098-5514pii
JVI.02103-14journal_volume
88pub_type
杂志文章abstract::Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3 (APOBEC3) proteins are encapsidated by assembling HIV-1 virions and edit viral cDNA in the next round of infection. Using alpha interferon (IFN-α)-treated monocyte-derived macrophages, we show that infrequent editing of HIV-1 reverse transcripts can als...
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abstract::To assess the RNA helicase activity of hepatitis C virus (HCV) nonstructural protein 3 (NS3), a polypeptide encompassing amino acids 1175 to 1657, which cover only the putative helicase domain, was expressed in Escherichia coli by a pET expression vector. The protein was purified to near homogeneity and assayed for RN...
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pub_type: 杂志文章
doi:10.1128/JVI.70.12.8477-8484.1996
更新日期:1996-12-01 00:00:00
abstract::Three plaque isolates of SV40 strain 777 and 1 plaque isolate of strain 776 were grown to high-titer stocks and serially passaged, undiluted, in monkey BS-C-1 cells. In each case, the serial passaging procedure resulted in the accumulation of closed-circular SV40 DNA molecules containing covalently linked sequences ho...
journal_title:Journal of virology
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doi:10.1128/JVI.12.3.492-500.1973
更新日期:1973-09-01 00:00:00
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journal_title:Journal of virology
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doi:10.1128/JVI.7.1.176-187.1971
更新日期:1971-01-01 00:00:00
abstract::The development of cancer vaccines requires approaches to induce expansion and functional differentiation of tumor antigen-specific cytotoxic T lymphocyte (CTL) effectors which posses cytolytic capability and produce cytokines. Efficient induction of such cells is hindered by the poor immunogenicity of tumor antigens ...
journal_title:Journal of virology
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doi:10.1128/jvi.77.13.7411-7424.2003
更新日期:2003-07-01 00:00:00
abstract::Although monkey B virus (herpesvirus simiae; BV) is common in all macaque species, fatal human infections appear to be associated with exposure to rhesus macaques (Macaca mulatta), suggesting that BV isolates from rhesus monkeys may be more lethal to nonmacaques than are BV strains indigenous to other macaque species....
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pub_type: 杂志文章
doi:10.1128/JVI.72.11.9224-9232.1998
更新日期:1998-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.60.3.910-919.1986
更新日期:1986-12-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.5.1845-1851.1989
更新日期:1989-05-01 00:00:00
abstract::Simian immunodeficiency virus (SIV) and human immunodeficiency virus (HIV) infections lead to rapid depletion of CD4(+) T cells from gut-associated lymphoid tissue (GALT). Although the administration of antiretroviral therapy (ART) has been shown to increase CD4(+) T-cell levels in the peripheral blood in both SIV and...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.5.2709-2719.2005
更新日期:2005-03-01 00:00:00
abstract::Hpt-13 is a Chinese hamster cell line deficient in hypoxanthine phosphoribosyltransferase (EC 2.4.2.8) and sensitive to a medium containing 10(-4) M hypoxanthine, 5.5 X 10(-6) M aminopterin, and 10(-4) M thymidine. In this cell line there is a high incidence of cells resistant to this selective medium after an incubat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.38.1.184-190.1981
更新日期:1981-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.2.696-704.1989
更新日期:1989-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00852-13
更新日期:2013-08-01 00:00:00
abstract::Arthropod genomes contain sequences derived from integrations of DNA and nonretroviral RNA viruses. These sequences, known as endogenous viral elements (EVEs), have been acquired over the course of evolution and have been proposed to serve as a record of past viral infections. Recent evidence indicates that EVEs can f...
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pub_type: 杂志文章
doi:10.1128/JVI.02124-18
更新日期:2019-03-05 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01508-19
更新日期:2020-01-17 00:00:00
abstract::The integrase protein of human immunodeficiency virus type 1 removes two nucleotides from the 3' ends of reverse-transcribed human immunodeficiency virus type 1 DNA (3' processing) and covalently inserts the processed ends into a target DNA (DNA strand transfer). Mutant integrase proteins that lack the amino-and/or ca...
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pub_type: 杂志文章
doi:10.1128/JVI.68.9.5911-5917.1994
更新日期:1994-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.7.4133-4141.1993
更新日期:1993-07-01 00:00:00
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pub_type: 临床试验,杂志文章,多中心研究
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.4.1297-1304.1988
更新日期:1988-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:1968-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.2.974-977.1989
更新日期:1989-02-01 00:00:00
abstract:UNLABELLED:Pneumonia virus of mice (PVM) is a natural rodent pathogen that replicates in bronchial epithelial cells and reproduces many clinical and pathological features of the more severe forms of disease associated with human respiratory syncytial virus. In order to track virus-target cell interactions during acute ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02279-15
更新日期:2015-11-04 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章,评审
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更新日期:2010-12-01 00:00:00
abstract::The binding of a recombinant soluble form of the measles virus (MV) hemagglutinin (sH) to cells expressing hybrid CD46/CD4 proteins was compared to that of purified virus. For binding of both ligands, both CD46 external short consensus repeats I and II (SCR I and II) in the natural order were essential. The addition o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.5.4157-4160.1997
更新日期:1997-05-01 00:00:00
abstract::The human immunodeficiency virus (HIV) integrase protein (IN) catalyzes two reactions required to integrate HIV DNA into the human genome: 3' processing of the viral DNA ends and integration. IN has three domains, the N-terminal zinc-binding domain, the catalytic core, and the C-terminal SH3 domain. Previously, it was...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.4.3176-3183.1999
更新日期:1999-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2017-08-24 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2013-10-01 00:00:00
abstract::Glycoprotein B (gB) of herpes simplex virus (HSV) is one of four glycoproteins essential for viral entry and cell fusion. Recently, paired immunoglobulin-like type 2 receptor (PILRalpha) was identified as a receptor for HSV type 1 (HSV-1) gB. Both PILRalpha and a gD receptor were shown to participate in HSV-1 entry in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00087-09
更新日期:2009-08-01 00:00:00