Abstract:
:Hpt-13 is a Chinese hamster cell line deficient in hypoxanthine phosphoribosyltransferase (EC 2.4.2.8) and sensitive to a medium containing 10(-4) M hypoxanthine, 5.5 X 10(-6) M aminopterin, and 10(-4) M thymidine. In this cell line there is a high incidence of cells resistant to this selective medium after an incubation with either ethyl methane sulfonate or adenovirus type 2 complete virions or their incomplete particles. The rate of reversion in the presence of these agents was 34-fold higher with ethyl methane sulfonate and 2.5- to 5.6-fold higher with adenovirus particles than the spontaneous rate of reversion. The revertant phenotypes were stable for many generations without selective pressure. All of the revertants tested recovered the hypoxanthine phosphoribosyltransferase activity. Most of them, however, carried an enzyme of lower activity and faster electrophoretic mobility than that of the wild type. The preferential reversion to this type of enzyme was observed among spontaneous revertants as well as among those induced by mutagenesis with ethyl methane sulfonate or exposure to viral particles. Our results suggest that adenovirus particles and ethyl methane sulfonate induce mutations at the hpt locus of Hpt-13 cells through similar mechanisms.
journal_name
J Viroljournal_title
Journal of virologyauthors
Marengo C,Mbikay M,Weber J,Thirion JPdoi
10.1128/JVI.38.1.184-190.1981subject
Has Abstractpub_date
1981-04-01 00:00:00pages
184-90issue
1eissn
0022-538Xissn
1098-5514journal_volume
38pub_type
杂志文章abstract::The 72-kDa immediate early 1 (IE1) protein encoded by human cytomegalovirus (hCMV) is a nuclearly localized promiscuous regulator of viral and cellular transcription. IE1 has long been known to associate with host mitotic chromatin, yet the mechanisms underlying this interaction have not been specified. In this study,...
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journal_title:Journal of virology
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2006-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.5.1606-1616.1988
更新日期:1988-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2009-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.6.3512-3526.1994
更新日期:1994-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.79.8.5163-5173.2005
更新日期:2005-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.10.7421-7428.1997
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.52.2.591-597.1984
更新日期:1984-11-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2004-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jvi.76.4.1959-1970.2002
更新日期:2002-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00639-08
更新日期:2008-10-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.66.3.1682-1687.1992
更新日期:1992-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.12.4007-4011.1987
更新日期:1987-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.10.5898-5905.1992
更新日期:1992-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:1992-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:2019-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2003-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:2016-07-27 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1989-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2009-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.19.10651-10657.2003
更新日期:2003-10-01 00:00:00