Abstract:
:The development of cancer vaccines requires approaches to induce expansion and functional differentiation of tumor antigen-specific cytotoxic T lymphocyte (CTL) effectors which posses cytolytic capability and produce cytokines. Efficient induction of such cells is hindered by the poor immunogenicity of tumor antigens and by the poor transduction efficiency of dendritic cells (DCs) with current nonreplicating vectors. We have investigated the use of influenza A virus, a potent viral inducer of CTLs, as a vector expressing the immunodominant HER-2 CTL epitope KIF (E75). For this purpose, an attenuated influenza A/PR8/34 virus with a truncated nonstructural (NS1) gene was generated containing the E75 epitope in its neuraminidase protein (KIF-NS virus). Stimulation of peripheral blood mononuclear cells from healthy donors and of tumor-associated lymphocytes from ovarian and breast cancer patients with DCs infected with KIF-NS virus (KIF-NS DC) induced CTLs that specifically recognized the peptide KIF and HER-2-expressing tumors in cytotoxicity assays and secreted gamma interferon (IFN-gamma) and interleukin-2 at recall with peptide. Priming with KIF-NS DCs increased the number of E75(+) CD45RO(+) cells by more than 10-fold compared to nonstimulated cells. In addition, KIF-NS virus induced high levels of IFN-alpha in DCs. This is the first report demonstrating induction of human epitope-specific CTLs against a tumor-associated antigen with a live attenuated recombinant influenza virus vector. Such vectors may provide a novel approach for tumor antigen delivery, lymphocyte activation, and differentiation in human cancer vaccine development.
journal_name
J Viroljournal_title
Journal of virologyauthors
Efferson CL,Schickli J,Ko BK,Kawano K,Mouzi S,Palese P,García-Sastre A,Ioannides CGdoi
10.1128/jvi.77.13.7411-7424.2003subject
Has Abstractpub_date
2003-07-01 00:00:00pages
7411-24issue
13eissn
0022-538Xissn
1098-5514journal_volume
77pub_type
杂志文章abstract::The Kaposi's sarcoma-associated herpesvirus (KSHV) K1 gene encodes a polypeptide bearing an immunoreceptor tyrosine-based activation motif (ITAM) that is constitutively active for ITAM-based signal transduction. Although ectopic overexpression of K1 in cultured fibroblasts can lead to growth transformation, in vivo th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.13.5891-5898.2001
更新日期:2001-07-01 00:00:00
abstract::Ribosomes from ghost-treated Escherichia coli can form in vitro initiation complexes but cannot translate added template RNA because of a defect in the 30S subunit; 16S rRNA is degraded. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.14.5.1310-1313.1974
更新日期:1974-11-01 00:00:00
abstract::Wild-type Sindbis virus (SINV) strain MRE16 efficiently infects Aedes aegypti midgut epithelial cells (MEC), but laboratory-derived neurovirulent SINV strain TE/5'2J infects MEC poorly. SINV determinants for MEC infection have been localized to the E2 glycoprotein. The E2 amino acid sequences of MRE16 and TE/5'2J diff...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02060-07
更新日期:2008-03-01 00:00:00
abstract::Chimeric bovine respiratory syncytial viruses (BRSV) expressing glycoproteins of bovine parainfluenza virus type 3 (BPIV-3) instead of BRSV glycoproteins were generated from cDNA. In the BRSV antigenome cDNA, the open reading frames of the major BRSV glycoproteins, attachment protein G and fusion protein F, were repla...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.19.9367-9377.2001
更新日期:2001-10-01 00:00:00
abstract::A coxsackievirus B3 (CB3) isolate adapted to growth in RD cells shows an alteration in cell tropism as a result of its capacity to bind a 70-kDa cell surface molecule expressed on these cells. We now show that this molecule is the complement regulatory protein, decay-accelerating factor (DAF) (CD55). Anti-DAF antibodi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.3.1903-1906.1995
更新日期:1995-03-01 00:00:00
abstract::Avian influenza A viruses, such as the highly pathogenic avian H5N1 viruses, sporadically enter the human population but often do not transmit between individuals. In rare cases, however, they establish a new lineage in humans. In addition to well-characterized barriers to cell entry, one major hurdle which avian viru...
journal_title:Journal of virology
pub_type: 杂志文章,评审
doi:10.1128/JVI.00980-13
更新日期:2013-07-01 00:00:00
abstract::The protein kinase inhibitor H7 blocks influenza virus replication, inhibits production of the matrix protein (M1), and leads to a retention of the viral ribonucleoproteins (vRNPs) in the nucleus at late times of infection (K. Martin and A. Helenius, Cell 67:117-130, 1991). We show here that production of assembled vR...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.4.1781-1786.2000
更新日期:2000-02-01 00:00:00
abstract::Expression of the immediate-early (IE) genes of herpes simplex virus (HSV) is specifically stimulated by a 65-kilodalton virion transcription factor (VF65 or VP16) that is introduced as a component of infecting virions. In both the IE175(ICP4) and IE110(ICP0) promoters, this activation requires an upstream cis-acting ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.6.2798-2812.1989
更新日期:1989-06-01 00:00:00
abstract::Plasmacytoid dendritic cells (pcDC) and myeloid dendritic cells (myDC) are shown to express CD4 and low levels of CCR5 and CXCR4, but only myDC express DC SIGN, a C-type lectin that binds human immunodeficiency virus but does not mediate virus entry. Both DC types were more susceptible to infection with a macrophage t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.14.6710-6713.2001
更新日期:2001-07-01 00:00:00
abstract::Studies on deoxyribonucleic acid purified from the granulosis virus of Trichoplusia ni have revealed the presence of a closed, double-stranded superhelix which sediments at 95S relative to relaxed circles (74S) and linear (60S) forms. Molecular weight estimates show that this insect virus deoxyribonucleic acid has a s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.9.4.710-713.1972
更新日期:1972-04-01 00:00:00
abstract::The herpes simplex virus type 1 UL28 gene contains a 785-amino-acid open reading frame that codes for an essential protein. Studies with temperature-sensitive mutants which map to the UL28 gene indicate that the UL28 gene product (ICP18.5) is required for packaging of viral DNA and for expression of viral glycoprotein...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.6.3470-3480.1993
更新日期:1993-06-01 00:00:00
abstract::The structures of DNAs present in various intracellular forms of simian virus 40 (SV40) nucleoprotein complexes were analyzed by micrococcal nuclease digestion. The results showed that the 70S SV40 chromatin was completely sensitive to nuclease digestion, whereas CsCl gradient-purified mature virion was completely res...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.44.2.603-609.1982
更新日期:1982-11-01 00:00:00
abstract::Cleavage of varicella-zoster virus DNA with the restriction endonucleases PstI, XbaI, and BglII resulted in 18, 22, and 20 fragments, respectively. Based on the molecular weights and molarities of these fragments, a molecular weight of 84 x 10(6) could be calculated for the varicella-zoster virus genome. In both the X...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.39.2.390-400.1981
更新日期:1981-08-01 00:00:00
abstract::The family Bunyaviridae is the most diversified family of RNA viruses. We describe a novel prototypic bunyavirus, tentatively named Gouléako virus, isolated from various mosquito species trapped in Côte d'Ivoire. The S segment comprised 1,087 nucleotides (nt), the M segment 3,188 nt, and the L segment 6,358 nt, consti...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00230-11
更新日期:2011-09-01 00:00:00
abstract::Among the five serine incorporator (SERINC) family members, SERINC5 (Ser5) was reported to strongly inhibit HIV-1 replication, which is counteracted by Nef. Ser5 produces 5 alternatively spliced isoforms: Ser5-001 has 10 putative transmembrane domains, whereas Ser5-004, -005, -008a, and -008b do not have the last one....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00137-17
更新日期:2017-04-28 00:00:00
abstract::Monoclonal antibody-resistant mutants have been widely used to estimate virus mutation frequencies. We demonstrate that standard virion neutralization inevitably underestimates monoclonal antibody-resistant mutant genome frequencies of vesicular stomatitis virus, due to phenotypic masking-mixing when wild-type (wt) vi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.12.5030-5036.1989
更新日期:1989-12-01 00:00:00
abstract::Although shrews are one of the largest groups of mammals, little is known about their role in the evolution and transmission of viral pathogens, including coronaviruses (CoVs). We captured 266 Asian house shrews (Suncus murinus) in Jiangxi and Zhejiang Provinces, China, during 2013 to 2015. CoV RNA was detected in 24 ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00764-17
更新日期:2017-08-10 00:00:00
abstract::The regulatory proteins Nef, Rev, and Tat of human immunodeficiency virus type 1 (HIV-1) are attractive targets for vaccine development, since induction of effective immune responses targeting these early proteins may best control virus replication. Here we investigated whether vaccination with biologically active Tat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.8.3800-3809.2002
更新日期:2002-04-01 00:00:00
abstract::Activation of host innate immune responses was studied in severe acute respiratory syndrome coronavirus (SCV)-infected human A549 lung epithelial cells, macrophages, and dendritic cells (DCs). In all cell types, SCV-specific subgenomic mRNAs were seen, whereas no expression of SCV proteins was found. No induction of c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.21.13800-13805.2005
更新日期:2005-11-01 00:00:00
abstract::Characterizations of genetic variations among hepatitis delta virus (HDV) isolates have focused principally on phylogenetic analysis of sequences, which vary by 30 to 40% among three genotypes and about 10 to 15% among isolates of the same genotype. The significance of the sequence differences has been unclear but cou...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.4.2806-2814.1998
更新日期:1998-04-01 00:00:00
abstract::During lytic infection, the genome of herpes simplex virus 1 (HSV-1) is associated with limited levels of histones but does not form a regular repeating nucleosomal structure. However, the previous observation that chromatin remodeling factors are recruited into viral replication compartments indicates that chromatin ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01575-08
更新日期:2008-12-01 00:00:00
abstract::Certain chicken cells that do not spontaneously release virus particles have been shown to produce a subgroup E avian RNA tumor virus, Rous-associated virus 60 (RAV-60), after infection with viruses of other subgroups. The nucleic acids of RAV-60 were analyzed for sequence homologies with the viral nucleic acids conta...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.15.6.1367-1377.1975
更新日期:1975-06-01 00:00:00
abstract::Infection by rat virus has been studied in cultures of rat embryo cells to evaluate the Margolis-Kilham hypothesis that the virus preferentially infects tissues with actively dividing cells. An enhancement of infection was seen in cultures infected 10 hr after fresh medium was added as compared to infection of station...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.4.6.872-878.1969
更新日期:1969-12-01 00:00:00
abstract::The hepadnavirus P gene contains amino acid sequences which share homology with all known RNases H. In this study, we made four mutants in which single amino acids of the duck hepatitis B virus (DHBV) RNase H region were altered. In two of them, amino acids at locations comprising the putative catalytic site were chan...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.9.6151-6156.1996
更新日期:1996-09-01 00:00:00
abstract::The Tf1 retrotransposon of Schizosaccharomyces pombe represents a group of eukaryotic long terminal repeat (LTR) retroelements that, based on their sequences, were predicted to use an RNA self-primer for initiating reverse transcription while synthesizing the negative-sense DNA strand. This feature is substantially di...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01370-08
更新日期:2008-11-01 00:00:00
abstract::The major immediate-early (MIE) gene of human cytomegalovirus (HCMV) expresses IE86, IE72, IE55, and IE18 mRNA by differential splicing. Reverse transcription-PCR with IE72-specific primers generated an 0.65-kb cDNA from HCMV-infected fibroblast RNA, which does not correspond to any known MIE cDNA. Nucleotide sequenci...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.7.3158-3167.2002
更新日期:2002-04-01 00:00:00
abstract::The E7 oncoprotein of human papillomavirus 16 (HPV16) transforms basal and suprabasal cervical epithelial cells and is a tumor-specific antigen in cervical carcinoma, to which immunotherapeutic strategies aimed at cytotoxic T-lymphocyte (CTL) induction are currently directed. By quantifying major histocompatibility co...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.13.5985-5997.2001
更新日期:2001-07-01 00:00:00
abstract::We have developed a system for producing murine leukemia virus (MLV) pseudotyped with human hepatitis B virus (HBV) large (L) and small (S) surface antigens (HBsAg) for targeting primary human hepatocytes. Using the MLV(HBV) pseudotype virus containing a beta-galactosidase reporter gene, we demonstrated that this pseu...
journal_title:Journal of virology
pub_type: 杂志文章,收录出版
doi:10.1128/jvi.76.2.912-917.2002
更新日期:2002-01-01 00:00:00
abstract::The coding properties of RNA transcribed in vitro by purified vaccinia cores have been investigated using Krebs ascites tumor cells, L cells, and reticulocyte lysates. Six to 10 proteins synthesized in vitro are separated on polyacrylamide gels by electrophoresis in the presence of sodium dodecyl sulfate. Their molecu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.15.6.1467-1474.1975
更新日期:1975-06-01 00:00:00
abstract::We have identified a cytomegalovirus virion protein capable of modulating the rapid induction of an interferon-like response in cells that follows virus binding and penetration. Functional genomics revealed a role for the major cytomegalovirus structural protein, pp65 (ppUL83), in counteracting this response. The unde...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.20.10995-11006.2004
更新日期:2004-10-01 00:00:00