Abstract:
:Marek's disease herpesvirus (MDV)-transformed lymphoblastoid tumor cell lines were characterized for the presence of the surface markers. Monoclonal antibodies were used for CD3 (T-cell receptor [TCR] complex), TCR1, TCR2, and TCR3, CD4, CD8, and Ia antigen by indirect fluorescence staining followed by microscopic examination or flow cytometry. The lymphoblastoid cell lines were obtained from tumors from chickens infected with MDV (n = 44) or from local lesions induced by inoculation of allogeneic, MDV-infected chick kidney cells (n = 56). Lymphocytes were harvested from these lesions between 4 and 16 days postinoculation and cultured in vitro to establish cell lines. All cell lines expressed Ia antigen and CD3 and/or TCR and thus are activated T cells. Most of the cell lines developed from tumors were CD4+ CD8-; only one cell line was negative for both markers. Sixteen percent of the cell lines were TCR3+, while the remainder were TCR2+. The cell lines developed from local lesions were much more heterogeneous: 45% were CD4- CD8+, 34% were CD4- CD8-, and only 21% were CD4+ CD8-. The number of TCR3+ cell lines was larger than expected for the CD4- CD8+ and CD4- CD8- cell lines, as judged from the presence of these cells in the blood. These results indicate that several subsets of T lymphocytes can be transformed by MDV, depending on the pathogenesis of infection. Activation of T cells as a consequence of the normal pathogenesis or by allogeneic stimulation seem to be a first important step in the process of transformation.
journal_name
J Viroljournal_title
Journal of virologyauthors
Schat KA,Chen CL,Calnek BW,Char Ddoi
10.1128/JVI.65.3.1408-1413.1991subject
Has Abstractpub_date
1991-03-01 00:00:00pages
1408-13issue
3eissn
0022-538Xissn
1098-5514journal_volume
65pub_type
杂志文章abstract::The complete genome sequences of two Sulfolobus spindle-shaped viruses (SSVs) from acidic hot springs in Kamchatka (Russia) and Yellowstone National Park (United States) have been determined. These nonlytic temperate viruses were isolated from hyperthermophilic Sulfolobus hosts, and both viruses share the spindle-shap...
journal_title:Journal of virology
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.23.12369-12375.2002
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abstract::Wild-type clones of reovirus serotypes 1 (Lang), 2 (Jones), and 3 (Dearing) were serially passaged in L cells at a high multiplicity of infection, and the virus population was examined at passage levels 2, 5, and 11 for the presence of temperature-sensitive (ts) mutants. By passage 11 all three serotypes contained ts ...
journal_title:Journal of virology
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doi:10.1128/JVI.34.1.285-287.1980
更新日期:1980-04-01 00:00:00
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journal_title:Journal of virology
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doi:10.1128/JVI.2.9.886-893.1968
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abstract::Vesicular stomatitis virus Indiana strain G protein (VSVind.G) is the most commonly used envelope glycoprotein to pseudotype lentiviral vectors (LV) for experimental and clinical applications. Recently, G proteins derived from other vesiculoviruses (VesG), for example, Cocal virus, have been proposed as alternative LV...
journal_title:Journal of virology
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02619-13
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01186-18
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abstract::Efforts to develop a vaccine against human immunodeficiency virus type 1 (HIV-1) are complicated by resistance of virus to neutralization. The neutralization resistance phenotype of HIV-1 has been linked to high infectivity. We studied the mechanisms determining this phenotype using clones of the T-cell-line-adapted (...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.1.560-570.2003
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.10.6733-6740.1996
更新日期:1996-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.7.6123-6127.1999
更新日期:1999-07-01 00:00:00
abstract::The structural aspects of SH antigen-containing particles were investigated. These studies confirmed the existence of a large spherical particle (ca. 43 nm) and smaller (ca. 20 nm) rod- and sphere-shaped particles. The large particle consists of an outer and inner membrane and a core of "nucleic acid" as seen by posit...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.6.5.685-689.1970
更新日期:1970-11-01 00:00:00
abstract::Infection of newborn Lewis rats with Borna disease virus (neonatal Borna disease [NBD]) results in cerebellar damage without the cellular inflammation associated with infections in later life. Purkinje cell (PC) damage has been reported for several models of early-life viral infection, including NBD; however, the time...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02245-06
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abstract::Analysis of the pronase-derived glycopeptides of isolated mumps virus glycoproteins revealed the presence of both complex and high-mannose-type oligosaccharides on the HN and F1 glycoproteins, whereas only high-mannose-type glycopeptides were detected on F2. Endoglycosidase F, a newly described glycosidase that cleave...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.47.2.354-362.1983
更新日期:1983-08-01 00:00:00
abstract::The E5 protein of human papillomavirus type 16 is a small, hydrophobic protein that localizes predominantly to membranes of the endoplasmic reticulum (ER). To define the orientation of E5 in these membranes, we employed a differential, detergent permeabilization technique that makes use of the ability of low concentra...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01968-09
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abstract::An alternatively spliced form of c-myb exists that encodes an additional 120 amino acids in chicken and 121 amino acids in human and mouse. These amino acids are encoded by an additional exon, termed exon 9A. This exon is not present in v-myb, and proteins containing these amino acids have never been tested for oncoge...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.8.6813-6821.1998
更新日期:1998-08-01 00:00:00
abstract::In December 2016, a low-pathogenic avian influenza (LPAI) A(H7N2) virus was identified to be the causative source of an outbreak in a cat shelter in New York City, which subsequently spread to multiple shelters in the states of New York and Pennsylvania. One person with occupational exposure to infected cats became in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00672-17
更新日期:2017-07-12 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.9.3.503-509.1972
更新日期:1972-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.10.4703-4708.1990
更新日期:1990-10-01 00:00:00
abstract::RNA recombination occurs frequently during replication of tombusviruses and carmoviruses, which are related small plus-sense RNA viruses of plants. The most common recombinants generated by these viruses are either defective interfering (DI) RNAs or chimeric satellite RNAs, which are thought to be generated by templat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.22.12033-12047.2003
更新日期:2003-11-01 00:00:00
abstract::Mice infected with myopathic coxsackievirus B1 Tucson (CVB1(T)) develop chronic inflammatory myopathy (CIM) consisting of hind limb weakness and inflammation. Amyopathic virus variants are infectious but attenuated for CIM. In this report, viral clones, chimeras, and sequencing were used to identify viral determinants...
journal_title:Journal of virology
pub_type: 杂志文章
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abstract::The simian picornaviruses were isolated from various primate tissues during the development of general tissue culture methods in the 1950s to 1970s or from specimens derived from primates used in biomedical research. Twenty simian picornavirus serotypes are recognized, and all are presently classified within the Enter...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.3.1244-1251.2002
更新日期:2002-02-01 00:00:00
abstract::Equine herpesvirus 1 (EHV-1, Kentucky A strain) preparations enriched for defective interfering particles (DIPs) can readily establish persistent infection. The UL1 gene, which is conserved in the genome of DIPs that mediate persistent infection, maps between nucleotides 1418 and 2192 (258 amino acids) from the L (lon...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.7.4122-4132.1993
更新日期:1993-07-01 00:00:00
abstract::We have previously described several human immunodeficiency virus type 1 (HIV-1) mutants that are characterized by an excessive-RNA-splicing phenotype and reduced virus particle production. In one of these mutants (NLD2up), the sequence of 5' splice site D2 was changed to a consensus splice donor site. This splice sit...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02295-07
更新日期:2008-02-01 00:00:00
abstract::Human T-cell lymphotropic virus type 1 (HTLV-1) is the etiological agent involved in the disease HTLV-1-associated myelopathy, or tropical spastic paraparesis (HAM/TSP). The pathogenesis of HAM/TSP is poorly understood, but it is probable that viral infection has an indirect, deleterious effect on neural function. In ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.12.8727-8736.1996
更新日期:1996-12-01 00:00:00
abstract::The phosphoprotein (P protein) of respiratory syncytial virus (RSV) is a key component of the viral RNA-dependent RNA polymerase complex. The protein is constitutively phosphorylated at the two clusters of serine residues (116, 117, and 119 [116/117/119] and 232 and 237 [232/237]). To examine the role of phosphorylati...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.21.10776-10784.2002
更新日期:2002-11-01 00:00:00
abstract::To study the tissue specificity of mouse mammary tumor virus (MMTV) gene expression, we developed two series of transgenic mice, containing the MMTV proviral DNA of mammary (GR) and kidney (C3H-K) origin. The expression pattern in the MMTV(GR) transgenic mice is very similar to that observed in infected animals, e.g.,...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.7.4580-4586.1992
更新日期:1992-07-01 00:00:00
abstract::Epstein-Barr virus (EBV) is a human herpesvirus capable of establishing a latent state in B lymphocytes. EBV's BZLF1 gene product plays a central role in regulating the switch from latency to productive infection. Here, we identify a sequence element, 5'-CAGGTA-3', called ZV, located at nucleotides -17 to -12 relative...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.2.867-877.2001
更新日期:2001-01-01 00:00:00
abstract::We applied deep sequencing technology to small RNA fractions from cells lytically infected with murine gammaherpesvirus 68 (gammaHV68) in order to define in detail small RNAs generated from a cluster of tRNA-related polycistronic structures located at the left end of the viral genome. We detected 10 new candidate micr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00707-10
更新日期:2010-10-01 00:00:00
abstract::The fundamental question of whether a primed immune system is capable of preventing latent gammaherpesvirus infection remains unanswered. Recent studies showing that vaccination can reduce acute replication and short-term latency but cannot alter long-term latency further call into question the possibility of achievin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.4.2522-2529.2003
更新日期:2003-02-01 00:00:00
abstract::The putative role of nitric oxide in the neuropathogenesis of Borna disease was investigated by determining changes in the expression of inducible nitric oxide synthase (iNOS) mRNA and constitutively expressed NOS (cNOS) mRNA in brains of Borna disease virus (BDV)-infected rats. iNOS mRNA was not detected in normal ra...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.10.5786-5791.1993
更新日期:1993-10-01 00:00:00