Abstract:
:Extracts prepared from either mouse cells or monkey cells were examined for the ability to support in vitro bovine papillomavirus type 1 (BPV1) DNA replication, and they were used in parallel as a source of host replication proteins for affinity chromatography. DNA synthesis exhibited an absolute requirement for BPV1 E1 protein. In contrast to previous observations, we found that low levels of E1 were highly efficient in initiating DNA replication in the absence of the BPV1 transcription factor E2. Surprisingly, COS-1 cell extract allowed a high rate of BPV1 DNA replication, supporting an efficient production of mature circular DNA molecules, whereas in mouse cell extracts, the replication products mostly consisted of replicative intermediates. Submitting the extracts to affinity chromatography allowed specific binding of DNA polymerase alpha-primase to E1 protein, up to a total depletion of the extract, regardless of the origin of the cell extract. Furthermore, replication protein A was not retained on E1 affinity columns, even when E2 was complexed with E1. These data confirm that the interactions between E1 and DNA polymerase alpha-primase do not exhibit cell-type specificity, as had already been suggested by data from in vivo and in vitro replication assays, but they imply that other cellular proteins may affect the level of E1-dependent replication.
journal_name
J Viroljournal_title
Journal of virologyauthors
Bonne-Andrea C,Santucci S,Clertant P,Tillier Fdoi
10.1128/JVI.69.4.2341-2350.1995subject
Has Abstractpub_date
1995-04-01 00:00:00pages
2341-50issue
4eissn
0022-538Xissn
1098-5514journal_volume
69pub_type
杂志文章abstract::Persistent infection with hepatitis C virus (HCV) is a major cause of chronic liver diseases. The aim of this study was to identify host cell factor(s) participating in the HCV replication complex (RC) and to clarify the regulatory mechanisms of viral genome replication dependent on the host-derived factor(s) identifi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02179-08
更新日期:2009-05-01 00:00:00
abstract::The principal neutralizing epitope of the human immunodeficiency virus type 1 (HIV-1) lies between two invariant cysteines in the third variable region (V3) of the viral envelope (gp120), and its amino acid sequence varies among different HIV-1 isolates. HIV-2 carries an analogous amino acid sequence between two cyste...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.7.4546-4550.1992
更新日期:1992-07-01 00:00:00
abstract::Kaposi's sarcoma-associated herpesvirus (KSHV) is linked to several neoplastic diseases: Kaposi's sarcoma, primary effusion lymphoma (PEL), and multicentric Castleman's disease (MCD). KSHV replicates actively, via a controlled gene expression program, but can also remain latent. It had been thought that the transition...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06617-11
更新日期:2012-04-01 00:00:00
abstract::Several aspects of the terminal stages of T4 head maturation were investigated using ts and am mutants blocked at single steps of the assembly pathway. We had previously found that cells infected with mutants of gene 13, e.g., tsN38 and amE609, accumulated both stable (10 to 20%)- and fragile (80%)-filled head precurs...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.17.2.550-567.1976
更新日期:1976-02-01 00:00:00
abstract::Murine gammaherpesvirus 68 (MHV68) infection of mice provides a tractable small-animal system for assessing viral requirements for establishment of and reactivation from latency. The M2 gene product has no homology to any known proteins but has been shown to play a role in both the establishment of MHV68 latency and r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00651-08
更新日期:2008-08-01 00:00:00
abstract::The stimulation of host macromolecular synthesis and induction into the cell cycle of serum-deprived G0-G1-arrested mouse embryo fibroblasts were examined after infection of resting cells with wild-type simian virus 40 or with viral mutants affecting T antigen (tsA58) or small t antigen (dl884). At various times after...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.37.2.802-812.1981
更新日期:1981-02-01 00:00:00
abstract::Our ability to rapidly respond to an emerging influenza pandemic is hampered somewhat by the lack of a susceptible small-animal model. To develop a more sensitive model, we pathotyped 18 low-pathogenic non-mouse-adapted influenza A viruses of human and avian origin in DBA/2 and C57BL/6 mice. The majority of the isolat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02444-09
更新日期:2010-08-01 00:00:00
abstract::The cloned murine cytolytic T-lymphocyte line IE1-IL and several sublines detect a murine cytomegalovirus immediate-early (IE) membrane determinant in conjunction with Ld class I major histocompatibility glycoprotein. The lines retained cytolytic activity, strict antigen specificity, and self-restriction even when ada...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.60.3.1125-1129.1986
更新日期:1986-12-01 00:00:00
abstract::The human cytomegalovirus (HCMV) UL138 protein downregulates the cell surface expression of the multidrug resistance-associated protein 1 (MRP1) transporter. We examined the genetic requirements within UL138 for MRP1 downregulation. We determined that the acidic cluster dileucine motif is essential for UL138-mediated ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00430-19
更新日期:2019-05-15 00:00:00
abstract::The cottontail rabbit papillomavirus (CRPV) a and b subtypes display a conserved E8 open reading frame encoding a 50-amino-acid hydrophobic protein, with structural similarities to the E5 transmembrane oncoprotein of genital human PVs (HPVs). CRPV E8 has been reported to play a role in papilloma growth but not to be e...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.10.4890-4900.2006
更新日期:2006-05-01 00:00:00
abstract::West Nile virus (WNV) is a neurotropic flavivirus and the leading cause of mosquito-borne encephalitis in the United States. Recent studies in humans have found that dysfunctional T cell responses strongly correlate with development of severe WNV neuroinvasive disease. However, the contributions of human dendritic cel...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00664-19
更新日期:2019-11-13 00:00:00
abstract::The p21 proteins of ras oncogenes are synthesized as precursors in the cytosol. After processing, which involves acylation, the products are associated with the plasma membrane in eucaryotic cells. The p21 overproduced in Escherichia coli, however, is not processed by acylation. A synthetic tetrapeptide of the p21 C t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.56.2.607-612.1985
更新日期:1985-11-01 00:00:00
abstract::We have developed a reconstituted system which models the events associated with human immunodeficiency virus type 1 (HIV-1) plus-strand transfer. These events include synthesis of plus-strand strong-stop DNA [(+) SSDNA] from a minus-strand DNA donor template covalently attached to human tRNA3Lys, tRNA primer removal,...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.6.4794-4805.1999
更新日期:1999-06-01 00:00:00
abstract::We have isolated a new type of temperature-sensitive mutant of simian virus 40 (SV40) that is capable of productive infection in permissive cells but not of maintenance of viral DNA integration in transformed cells at the conditional temperature. Virus development is induced when cells transformed by this mutant are s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.19.2.374-381.1976
更新日期:1976-08-01 00:00:00
abstract::The DNase/alkaline exonuclease (AE) genes are well conserved in all herpesvirus families, but recent studies have shown that the AE proteins of gammaherpesviruses such as Epstein-Barr virus (EBV) and Kaposi's sarcoma-associated herpesvirus (KSHV) exhibit an additional function which shuts down host protein synthesis. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01946-07
更新日期:2008-03-01 00:00:00
abstract::Five gibbon ape leukemia virus substrains (two from gibbons with lymphocytic leukemia and three from gibbons with myelogenous leukemia) were examined for unique genomic sequences specific for each form of leukemia. By using sequential adsorption procedures, the genome from each gibbon ape leukemia virus was fractionat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.35.2.400-408.1980
更新日期:1980-08-01 00:00:00
abstract::HB-3, a temperate bacteriophage of Streptococcus pneumoniae, synthesizes its own murein hydrolase activity when multiplying on cultures of pneumococcus. The enzyme (HBL) was purified and biochemically characterized as an N-acetylmuramoyl-L-alanine amidase of 36,000 daltons, and a 2.1-kilobase DraI fragment containing ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.1.137-142.1990
更新日期:1990-01-01 00:00:00
abstract::A panel of 28 insertion mutants of the human immunodeficiency virus type 1 (HIV-1) Gag precursor (Pr55Gag) was constructed by linker-insertion mutagenesis and expressed in recombinant baculovirus-infected insect cells. One set of 14 mutants carried the normal N-myristylation signal; the other set constituted their non...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.1.111-122.1994
更新日期:1994-01-01 00:00:00
abstract::Rhesus monkey rhadinovirus (RRV), a simian gamma-2 herpesvirus closely related to the Kaposi sarcoma-associated herpesvirus, replicates lytically in cultured rhesus monkey fibroblasts and establishes persistence in B cells. Overlapping cosmid clones were generated that encompass the entire 130-kilobase-pair genome of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.3.1549-1562.2006
更新日期:2006-02-01 00:00:00
abstract::Zyxin, a focal adhesion molecule, interacts specifically with the E6 protein from human papillomavirus (HPV) type 6 in a yeast two-hybrid screen of a cDNA library prepared from human keratinocytes. Zyxin does not interact significantly with E6 proteins from HPV types 11, 16, or 18. The interaction was confirmed by in ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.23.11791-11802.2001
更新日期:2001-12-01 00:00:00
abstract::SF162 is a primary (PR), non-syncytium-inducing, macrophagetropic human immunodeficiency virus type 1 (HIV-1) clade B isolate which is resistant to antibody-mediated neutralization. Deletion of the first or second hypervariable envelope gp120 region (V1 or V2 loop, respectively) of this virus does not abrogate its abi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.10.7840-7845.1998
更新日期:1998-10-01 00:00:00
abstract::It is shown that the plasmid prophage P1 can be displaced by a superinfecting P1 phage in Escherichia coli recA(P1) lysogens. Six widely separated phage markers were used to distinguish between residual recombination and total substitution. It is further shown that superinfection of recA lysogens can lead to loss of b...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.31.2.277-280.1979
更新日期:1979-08-01 00:00:00
abstract::The cancer-related mucin-type carbohydrate neoantigen Tn was found on gp160 and gp120 of human immunodeficiency virus (HIV). Immunoglobulin G (IgG) and IgM monoclonal antibodies (MAbs) against Tn neutralized infection with cell-free virus and blocked fusion between HIV-infected and uninfected cells. This inhibition wa...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.12.6461-6467.1991
更新日期:1991-12-01 00:00:00
abstract:UNLABELLED:Foreskin is the principal site of heterosexual HIV-1 infection in men. However, little is known about HIV-1-specific immune responses or inflammation in foreskin. To the best of our knowledge, no previous studies have assessed immune responses to candidate HIV-1 vaccines in foreskin. Using the rhesus monkey ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03771-13
更新日期:2014-04-01 00:00:00
abstract::The membrane fusion events which initiate human immunodeficiency virus type 1 (HIV-1) infection and promote cytopathic syncytium formation in infected cells commence with the binding of the HIV envelope glycoprotein (Env) to CD4 and an appropriate coreceptor. Here, we show that HIV Env-coreceptor interactions activate...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.13.7138-7147.2004
更新日期:2004-07-01 00:00:00
abstract::The kinetics of retroviral DNA and RNA synthesis are parameters vital to understanding viral growth, especially for human immunodeficiency virus (HIV), which encodes several of its own regulatory genes. We have established a single-cycle growth condition for HIV in H9 cells, a human CD4+ lymphocyte line. The full-leng...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.9.3708-3713.1989
更新日期:1989-09-01 00:00:00
abstract::Flavopiridol, roscovitine, and other inhibitors of Cyclin-Dependent Kinases (CDK) inhibit the replication of a variety of viruses in vitro while proving nontoxic in human clinical trials of their effects against cancer. Consequently, these and other Pharmacological CDK inhibitors (PCIs) have been proposed as potential...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.17.9352-9365.2004
更新日期:2004-09-01 00:00:00
abstract::The recently developed subgenomic hepatitis C virus (HCV) replicons were limited by the fact that the sequence encoding the structural proteins was missing. Therefore, important information about a possible influence of these proteins on replication and pathogenesis and about the mechanism of virus formation could not...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.8.4008-4021.2002
更新日期:2002-04-01 00:00:00
abstract::Mutants of polyomavirus with altered host specificities were isolated in undifferentiated C2 myoblast cells (L. Ricci, R. Maione, C. Passananti, A. Felsani, and P. Amati, J. Virol. 66:7153-7158, 1992). The mutations responsible for this phenotype belonged to either of the two classes: a large duplication of the enhanc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.2.1196-1199.1994
更新日期:1994-02-01 00:00:00
abstract::Dengue virus (DENV) is an important human pathogen, especially in the tropical and subtropical parts of the world, causing considerable morbidity and mortality. DENV replication occurs in the cytoplasm; however, a high proportion of nonstructural protein 5 (NS5), containing methyltransferase (MTase) and RNA-dependent ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03083-12
更新日期:2013-04-01 00:00:00