Abstract:
:The abilities to infect and transmit efficiently among humans are essential for a novel influenza A virus to cause a pandemic. To evaluate the pandemic potential of widely disseminated H5N1 influenza viruses, a ferret contact model using experimental groups comprised of one inoculated ferret and two contact ferrets was used to study the transmissibility of four human H5N1 viruses isolated from 2003 to 2006. The effects of viral pathogenicity and receptor binding specificity (affinity to synthetic sialosaccharides with alpha2,3 or alpha2,6 linkages) on transmissibility were assessed. A/Vietnam/1203/04 and A/Vietnam/JP36-2/05 viruses, which possess "avian-like" alpha2,3-linked sialic acid (SA) receptor specificity, caused neurological symptoms and death in ferrets inoculated with 10(3) 50% tissue culture infectious doses. A/Hong Kong/213/03 and A/Turkey/65-596/06 viruses, which show binding affinity for "human-like" alpha2,6-linked SA receptors in addition to their affinity for alpha2,3-linked SA receptors, caused mild clinical symptoms and were not lethal to the ferrets. No transmission of A/Vietnam/1203/04 or A/Turkey/65-596/06 virus was detected. One contact ferret developed neutralizing antibodies to A/Hong Kong/213/03 but did not exhibit any clinical signs or detectable virus shedding. In two groups, one of two naïve contact ferrets had detectable virus after 6 to 8 days when housed together with the A/Vietnam/JP36-2/05 virus-inoculated ferrets. Infected contact ferrets showed severe clinical signs, although little or no virus was detected in nasal washes. This limited virus shedding explained the absence of secondary transmission from the infected contact ferret to the other naïve ferret that were housed together. Our results suggest that despite their receptor binding affinity, circulating H5N1 viruses retain molecular determinants that restrict their spread among mammalian species.
journal_name
J Viroljournal_title
Journal of virologyauthors
Yen HL,Lipatov AS,Ilyushina NA,Govorkova EA,Franks J,Yilmaz N,Douglas A,Hay A,Krauss S,Rehg JE,Hoffmann E,Webster RGdoi
10.1128/JVI.00170-07subject
Has Abstractpub_date
2007-07-01 00:00:00pages
6890-8issue
13eissn
0022-538Xissn
1098-5514pii
JVI.00170-07journal_volume
81pub_type
杂志文章abstract::The hemagglutinin (HA), a glycoprotein on the surface of influenza A virus (IAV), initiates the virus life cycle by binding to terminal sialic acid (SA) residues on host cells. The HA gradually accumulates amino acid substitutions that allow IAV to escape immunity through a mechanism known as antigenic drift. We recen...
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pub_type: 杂志文章
doi:10.1128/JVI.78.24.14033-14038.2004
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journal_title:Journal of virology
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doi:10.1128/JVI.15.1.115-120.1975
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pub_type: 杂志文章
doi:10.1128/jvi.78.3.1256-1262.2004
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abstract::Two murine leukemia viruses were isolated from JLS-V9 cells which had been infected with Rauscher plasma virus. One virus was XC positive and failed to grow on mink or cat cells and thus was an ecotropic virus. The other virus formed cytopathic foci on mink cells, was XC negative, and fell into the mink cell focus-for...
journal_title:Journal of virology
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doi:10.1128/JVI.45.3.995-1003.1983
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journal_title:Journal of virology
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doi:10.1128/JVI.4.5.603-609.1969
更新日期:1969-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.8.4442-4448.1991
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journal_title:Journal of virology
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doi:10.1128/JVI.00053-16
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doi:10.1128/JVI.79.1.472-485.2005
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.29.1.34-42.1979
更新日期:1979-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.2.593-599.2000
更新日期:2000-01-01 00:00:00
abstract::Venezuelan equine encephalitis virus (VEE) is an important equine and human pathogen of the Americas. In the adult mouse model, cDNA-derived, virulent V3000 inoculated subcutaneously (s.c.) causes high-titer peripheral replication followed by neuroinvasion and lethal encephalitis. A single change (G to A) at nucleotid...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.8.3706-3718.2001
更新日期:2001-04-01 00:00:00
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doi:10.1128/JVI.00942-12
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.1.2.362-367.1967
更新日期:1967-04-01 00:00:00
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journal_title:Journal of virology
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.8.4063-4069.1991
更新日期:1991-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00103-06
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.52.1.129-135.1984
更新日期:1984-10-01 00:00:00
abstract::Respiratory syncytial virus (RSV) infection causes substantial morbidity and some deaths in the young and elderly worldwide. There is no safe and effective vaccine available, although it is possible to reduce the hospitalization rate for high-risk children by anti-RSV antibody prophylaxis. RSV has been shown to modify...
journal_title:Journal of virology
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2002-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.9.3506-3508.1988
更新日期:1988-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2002-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.14.7465-7477.2004
更新日期:2004-07-01 00:00:00
abstract::Lysyl-tRNA synthetase (LysRS) is packaged into human immunodeficiency virus type 1 (HIV-1) via its interaction with Gag, and this enzyme facilitates the selective packaging of tRNA(3)(Lys), the primer for initiating reverse transcription, into HIV-1. The Gag/LysRS interaction is detected at detergent-resistant membran...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.14.7553-7564.2004
更新日期:2004-07-01 00:00:00
abstract::Rigorous investigation of many functions encoded by cytomegaloviruses (CMVs) requires analysis in the context of virus-host interactions. To facilitate the construction of rhesus CMV (RhCMV) mutants for in vivo studies, a bacterial artificial chromosome (BAC) containing an enhanced green fluorescent protein (EGFP) cas...
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pub_type: 杂志文章
doi:10.1128/jvi.77.9.5073-5083.2003
更新日期:2003-05-01 00:00:00