Abstract:
:Isoelectrofocusing of nonionic-detergent-disrupted flaviviruses separated the envelope glycoprotein of 53,000 to 58,000 daltons and the nucleocapsid protein of 14,000 daltons. The envelope protein and nucleocapsid protein were isolated at isoelectric points of pI 7.8 and 10.3, respectively. The antigenic determinants of St. Louis encephalitis, Japanese encephalitis, and dengue virus envelope and nucleocapsid proteins were examined by solid-phase competition radioimmunoassay. By the appropriate selection of antiserum and competing proteins, it was possible to distinguish type-specific, complex-reactive and flavivirus group-reactive antigenic determinants. The envelope glycoproteins of St. Louis encephalitis, Japanese encephalitis, and dengue viruses were found to contain each of these three classes of antigenic determinants. Most of the determinants on the envelope protein were type specific, some were complex reactive, and a small fraction were flavivirus group reactive. The nucleocapsid protein contained only flavivirus group-reactive antigenic determinants.
journal_name
J Viroljournal_title
Journal of virologyauthors
Trent DWdoi
10.1128/JVI.22.3.608-618.1977subject
Has Abstractpub_date
1977-06-01 00:00:00pages
608-18issue
3eissn
0022-538Xissn
1098-5514journal_volume
22pub_type
杂志文章abstract::Human papillomavirus type 1 (HPV1) E4 protein is associated with cytoplasmic and nuclear inclusions in productively infected keratinocytes. Here we have used transient expression of HPV1 E4 (also known as E1E4) protein in keratinocytes to reproduce formation of E4 inclusions. Immunofluorescence analysis showed that pr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.1.673-684.2003
更新日期:2003-01-01 00:00:00
abstract::Type 3 reovirus inhibits L cell DNA synthesis, whereas type 1 reovirus exerts little or no effect on L cell DNA synthesis. By using recombinant viruses containing both type 1 and type 3 double-standard RNA segments, we determined that one double-stranded RNA segment, the reovirus type 3 S1 double-stranded RNA segment ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.38.1.389-392.1981
更新日期:1981-04-01 00:00:00
abstract::Glycoprotein B (gB) of herpes simplex virus (HSV) is one of four glycoproteins essential for viral entry and cell fusion. Recently, paired immunoglobulin-like type 2 receptor (PILRalpha) was identified as a receptor for HSV type 1 (HSV-1) gB. Both PILRalpha and a gD receptor were shown to participate in HSV-1 entry in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00087-09
更新日期:2009-08-01 00:00:00
abstract::Host-virus interactions control disease progression in human immunodeficiency virus-infected human beings and in nonhuman primates infected with simian or simian/human immunodeficiency viruses (SHIV). These interactions evolve rapidly during acute infection and are key to the mechanisms of viral persistence and AIDS. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.12.10236-10244.1999
更新日期:1999-12-01 00:00:00
abstract::A complete endogenous type D viral genome has been isolated from a baboon genomic library. The provirus, simian endogenous retrovirus (SERV), is 8,393 nucleotides long and contains two long terminal repeats and complete genes for gag, pro, pol, and env. The primer binding site is complementary to tRNA(Lys)3, like in l...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.5.3666-3676.1997
更新日期:1997-05-01 00:00:00
abstract::The pandemic threat posed by emerging zoonotic influenza A viruses necessitates development of antiviral agents effective against various antigenic subtypes. Human monoclonal antibody (hMAb) targeting the hemagglutinin (HA) stalk offers a promising approach to control influenza virus infections. Here, we investigated ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01284-16
更新日期:2016-11-14 00:00:00
abstract::An E1-E3-deleted recombinant adenovirus vector expressing the hybrid protein TetR-KRAB has been produced. In this virus, AdTG9562, the E2 transcription is regulated by TetR-KRAB and tetO sequences inserted in cis. In the absence of tetracycline, a strong reduction in E2A gene expression, viral DNA replication, and lat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.4.3307-3311.1997
更新日期:1997-04-01 00:00:00
abstract::We have previously reported that infection of 9- to 13-day-old chicken embryos with RAV-1 results in rapid development of a novel B-cell lymphoma in which proviral insertion has activated expression of the c-myb gene (E. Pizer and E. H. Humphries, J. Virol. 63:1630-1640, 1989). The biological properties of these B-cel...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.1.512-523.1992
更新日期:1992-01-01 00:00:00
abstract::HLA-B*52:01 is strongly associated with protection against HIV disease progression. However, the mechanisms of HLA-B*52:01-mediated immune control have not been well studied. We here describe a cohort with a majority of HIV C-clade-infected individuals from Delhi, India, where HLA-B*52:01 is highly prevalent (phenotyp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02025-19
更新日期:2020-06-16 00:00:00
abstract::Our previous studies have argued persuasively that in murine sarcoma virus ts110 (MuSVts110) the gag and mos genes are fused out of frame due to a approximately 1.5-kilobase (kb) deletion of wild-type murine sarcoma virus 349 (MuSV-349) viral information. As a consequence of this deletion, infected cells grown at 39 d...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.53.2.624-633.1985
更新日期:1985-02-01 00:00:00
abstract::The insertion of a particular phi X DNA sequence in the plasmid pACYC177 strongly decreased the capacity of Escherichia coli cells containing such a plasmid to propagate bacteriophage phi X174. The smallest DNA sequence tested that showed the effect was the HindII fragment R4. This fragment does not code for a complet...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.42.1.1-11.1982
更新日期:1982-04-01 00:00:00
abstract::The deployment of adenovirus serotype 5 (Ad5)-based vectors is hampered by preexisting immunity. When such vectors are delivered intravenously, hepatocyte transduction is mediated by the hexon-coagulation factor X (FX) interaction. Here, we demonstrate that human sera efficiently block FX-mediated cellular binding and...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01878-08
更新日期:2009-01-01 00:00:00
abstract::The E7 oncoprotein of human papillomavirus 16 (HPV16) transforms basal and suprabasal cervical epithelial cells and is a tumor-specific antigen in cervical carcinoma, to which immunotherapeutic strategies aimed at cytotoxic T-lymphocyte (CTL) induction are currently directed. By quantifying major histocompatibility co...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.13.5985-5997.2001
更新日期:2001-07-01 00:00:00
abstract::The human cytomegalovirus (HCMV) major immediate-early (MIE) enhancer contains five functional cyclic AMP (cAMP) response elements (CRE). Because the CRE in their native context do not contribute appreciably to MIE enhancer/promoter activity in lytically infected human fibroblasts and NTera2 (NT2)-derived neurons, we ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01524-06
更新日期:2007-06-01 00:00:00
abstract::Infection of cells with poliovirus induces a massive intracellular membrane reorganization to form vesicle-like structures where viral RNA replication occurs. The mechanism of membrane remodeling remains unknown, although some observations have implicated components of the cellular secretory and/or autophagy pathways....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01820-06
更新日期:2007-01-01 00:00:00
abstract::The human immunodeficiency virus (HIV) and the closely related simian immunodeficiency virus (SIV) induce profound immune dysfunction in primate species. The present studies show that cell populations infected in vitro with SIV exhibit increases in major histocompatibility complex (MHC) class II antigen expression. Ce...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.5.1421-1426.1987
更新日期:1987-05-01 00:00:00
abstract::During EBV infection, lytic DNA replication activates late gene expression in trans via an uncharacterized pathway. In this study, we mapped the target of this regulatory cascade to a variant TATA box (TATTAAA) and the 3' flanking region within the core promoter of the BcLF1 gene. The inherent late activity of this co...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.10.8338-8343.1998
更新日期:1998-10-01 00:00:00
abstract::E26 is a replication-defective avian retrovirus that causes an erythroblastic leukemia in vivo and transforms hematopoietic precursor cells of both the erythroid and the myeloid lineages in vitro. The E26 genome contains two sets of cell-derived sequences, ets and myb. myb sequences are also present in avian myeloblas...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.1.398-402.1989
更新日期:1989-01-01 00:00:00
abstract::Cleavage of varicella-zoster virus DNA with the restriction endonucleases PstI, XbaI, and BglII resulted in 18, 22, and 20 fragments, respectively. Based on the molecular weights and molarities of these fragments, a molecular weight of 84 x 10(6) could be calculated for the varicella-zoster virus genome. In both the X...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.39.2.390-400.1981
更新日期:1981-08-01 00:00:00
abstract::Foot-and-mouth disease (FMD), which is caused by FMD virus (FMDV), remains a major plague among cloven-hoofed animals worldwide, and its outbreak often has disastrous socioeconomic consequences. A live-attenuated FMDV vaccine will greatly facilitate the global control and eradication of FMD, but a safe and effective a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00990-20
更新日期:2020-07-30 00:00:00
abstract::To examine the mode of transmission of BK polyomavirus (BKV), urine samples were collected from Japanese-Americans in Los Angeles and from other southern Californians. Subtype I was the main subtype found in samples from both groups. The subtype I subgroup Ib-2, which is predominant in Europe, was the primary subgroup...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01018-07
更新日期:2007-12-01 00:00:00
abstract::Vaccinia extracellular enveloped virus (EEV) is critical for cell-to-cell and long-range virus spread both in vitro and in vivo. The B5R gene encodes an EEV-specific type I membrane protein that is essential for efficient EEV formation. The majority of the B5R ectodomain consists of four domains with homology to short...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.10.6260-6271.2005
更新日期:2005-05-01 00:00:00
abstract::A polymorphism in the gene encoding CCR2 is associated with a delay in progression to AIDS in human immunodeficiency virus (HIV)-infected individuals. The polymorphism, CCR2-64I, changes valine 64 of CCR2 to isoleucine. However, it is not clear whether the effect on AIDS progression results from the amino acid change ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.3.2450-2459.1999
更新日期:1999-03-01 00:00:00
abstract::Long-term ovine fibroblast cultures contain replicative-competent lentiviruses in a latent form. This in vitro phenomenon, never described previously for lentiviruses, was clearly demonstrated by activating the expression of latent viruses with various inducing cell treatments, some of which were efficient in inducing...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.52.2.680-682.1984
更新日期:1984-11-01 00:00:00
abstract::The complete nucleotide sequences of genomic RNA1 (9,407 nucleotides [nt]) and RNA2 (8,223 nt) of Sweet potato chlorotic stunt virus (SPCSV; genus Crinivirus, family Closteroviridae) were determined, revealing that SPCSV possesses the second largest identified positive-strand single-stranded RNA genome among plant vir...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.18.9260-9270.2002
更新日期:2002-09-01 00:00:00
abstract::Five gibbon ape leukemia virus substrains (two from gibbons with lymphocytic leukemia and three from gibbons with myelogenous leukemia) were examined for unique genomic sequences specific for each form of leukemia. By using sequential adsorption procedures, the genome from each gibbon ape leukemia virus was fractionat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.35.2.400-408.1980
更新日期:1980-08-01 00:00:00
abstract::Langat virus (LGT), the naturally attenuated member of the tick-borne encephalitis virus (TBEV) complex, was tested extensively in clinical trials as a live TBEV vaccine and was found to induce a protective, durable immune response; however, it retained a low residual neuroinvasiveness in mice and humans. In order to ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.3.1427-1439.2006
更新日期:2006-02-01 00:00:00
abstract::ZAM is an env-containing member of the gypsy family of retrotransposons that represents a possible retrovirus of invertebrates. In this paper, we traced ZAM mobilization to get information about a potential path a retroelement may take to reach the germ line of its host. In situ hybridization on whole-mount tissues an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.22.10658-10669.2000
更新日期:2000-11-01 00:00:00
abstract::Despite intensive study, the mechanism by which many retroviruses complete reverse transcription has remained unclear. Most retroviruses and all lentiviruses fail to synthesize a full-length second strand of the viral cDNA (plus strand) efficiently in infected cells. For human immunodeficiency virus type 1, we find in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.6.3938-3944.1995
更新日期:1995-06-01 00:00:00
abstract::The single-stranded DNA containing the Moloney murine leukemia virus origin for plus-strand synthesis was cloned in M13mp2 and used as a template for avian myeloblastosis virus reverse transcriptase in the presence of Moloney RNA which had been treated with pancreatic RNase A. The RNA pieces containing the polypurine ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.49.3.686-691.1984
更新日期:1984-03-01 00:00:00