Abstract:
:Kaposi's sarcoma-associated herpesvirus (KSHV) is a recently discovered human gamma herpesvirus strongly implicated in AIDS-related neoplasms. We report here the identification in the KSHV genome of a gene for a protein designated K-bZIP and belonging to the basic-leucine zipper (bZIP) family of transcription factors. K-bZIP shows significant homology to BZLF1, which plays a key role in the replication and reactivation of Epstein-Barr virus. K-bZIP is a homodimerizing protein of 237 amino acids with a prototypic bZIP domain at the C terminus. The N-terminal portion of K-bZIP is derived from the K8 open reading frame which, through in-frame splicing, adjoins the ZIP domain. This structure was revealed by rapid analysis of cDNA ends, followed by cloning of the entire cDNA. A 1.35-kb transcript encoding K-bZIP was detected in BCBL-1 cells treated with 12-O-tetradecanoylphorbol-13-acetate. The synthesis of this transcript was blocked by the protein synthesis inhibitor cycloheximide but not by the viral DNA synthesis inhibitor phosphonoacetate, a result which classifies it as an early lytic gene. RNase protection analysis further mapped the major transcription start site for the 1.35-kb K-bZIP mRNA and identified two other splice variants which encode proteins with the N-terminal portion of K-bZIP but lacking the C-terminal ZIP domain. Full-length K-bZIP forms dimers with itself, and the C terminus encompassing the ZIP domain is required for this process. Our studies set the stage for understanding the role of K-bZIP in the replication and reactivation of the KSHV genome.
journal_name
J Viroljournal_title
Journal of virologyauthors
Lin SF,Robinson DR,Miller G,Kung HJdoi
10.1128/JVI.73.3.1909-1917.1999subject
Has Abstractpub_date
1999-03-01 00:00:00pages
1909-17issue
3eissn
0022-538Xissn
1098-5514journal_volume
73pub_type
杂志文章abstract::Brome mosaic virus (BMV), a member of the alphavirus-like super-family of positive-strand RNA viruses, encodes two proteins required for viral RNA replication: 1a and 2a. 1a contains m7G methyltransferase- and helicase-like domains, while 2a contains a polymerase (pol)-like core flanked by N- and C-terminal extensions...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.7.4729-4736.1996
更新日期:1996-07-01 00:00:00
abstract:UNLABELLED:Pneumonia virus of mice (PVM) is a natural rodent pathogen that replicates in bronchial epithelial cells and reproduces many clinical and pathological features of the more severe forms of disease associated with human respiratory syncytial virus. In order to track virus-target cell interactions during acute ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02279-15
更新日期:2015-11-04 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.10.5043-5050.2002
更新日期:2002-05-01 00:00:00
abstract:UNLABELLED:Despite the nutritional and health benefits of breast milk, breast milk can serve as a vector for mother-to-child HIV transmission. Most HIV-infected infants acquire HIV through breastfeeding. Paradoxically, most infants breastfed by HIV-positive women do not become infected. This is potentially attributed t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01702-15
更新日期:2015-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.11.8182-8186.1996
更新日期:1996-11-01 00:00:00
abstract::The Tf1 retrotransposon of Schizosaccharomyces pombe represents a group of eukaryotic long terminal repeat (LTR) retroelements that, based on their sequences, were predicted to use an RNA self-primer for initiating reverse transcription while synthesizing the negative-sense DNA strand. This feature is substantially di...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01370-08
更新日期:2008-11-01 00:00:00
abstract::Treatment of [3H]glucosamine-labeled Friend mink cell focus-forming virus (FrMCF) gp70 with excess peptide:N-glycanase F (PNGase F) resulted in removal of the expected seven N-linked oligosaccharide chains; however, approximately 10% of the glucosamine label was retained in the resulting 49,000-Mr (49K) product. For [...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.3.1016-1021.1988
更新日期:1988-03-01 00:00:00
abstract:UNLABELLED:Human cytomegalovirus (hCMV) infection is characterized by a vast expansion of resting effector-type virus-specific T cells in the circulation. In mice, interleukin-7 receptor α (IL-7Rα)-expressing cells contain the precursors for long-lived antigen-experienced CD8(+) T cells, but it is unclear if similar me...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02003-14
更新日期:2015-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.7.6182-6187.1999
更新日期:1999-07-01 00:00:00
abstract::To study the tissue specificity of mouse mammary tumor virus (MMTV) gene expression, we developed two series of transgenic mice, containing the MMTV proviral DNA of mammary (GR) and kidney (C3H-K) origin. The expression pattern in the MMTV(GR) transgenic mice is very similar to that observed in infected animals, e.g.,...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.7.4580-4586.1992
更新日期:1992-07-01 00:00:00
abstract::Suppression of interferon signaling is of paramount importance to a virus. Interferon signaling significantly reduces or halts the ability of a virus to replicate; therefore, viruses have evolved sophisticated mechanisms that suppress activation of the interferon pathway or responsiveness of the infected cell to inter...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02484-16
更新日期:2017-03-29 00:00:00
abstract::To further define the transcriptional regulation of the P38 promoter in the minute virus of mice (MVM) genome, we constructed a series of internal deletion and linker scanning mutations. The mutant P38 constructs were assayed for transcriptional activity in vitro by primer extension analysis with nuclear extracts from...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.6.3776-3783.1992
更新日期:1992-06-01 00:00:00
abstract::The role of glycosylation and of disulfide bonds in the formation of the native structure of the Sendai virus hemagglutinin-neuraminidase (HN) and fusion (F0) glycoproteins was studied. In contrast to the HN and F0 synthesized in vivo, the proteins made from pSP6 transcripts in reticulocyte lysates, whether glycosylat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.2.892-900.1989
更新日期:1989-02-01 00:00:00
abstract::Herpesviruses establish latency in suitable host cells after primary infection and persist in their host organisms for life. Most of the viral genes are silenced during latency, also enabling the virus to escape from an immune response. This study addresses the control of viral gene silencing by epigenetic mechanisms,...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00164-11
更新日期:2011-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.5.2956-2963.2003
更新日期:2003-03-01 00:00:00
abstract::In an attempt to generate broadly cross-reactive, neutralizing monoclonal antibodies (MAbs) to simian immunodeficiency virus (SIV), we compared two immunization protocols using different preparations of oligomeric SIV envelope (Env) glycoproteins. In the first protocol, mice were immunized with soluble gp140 (sgp140) ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.17.7922-7935.2000
更新日期:2000-09-01 00:00:00
abstract::The ability of vaccinia virus to replicate in HeLa cells which had been previously infected with adenovirus type 2 (Ad2) was studied in order to gain insight into the mechanism by which adenovirus inhibits the expression of host cell functions. Vaccinia virus was employed in these studies because it replicates in the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.7.2.208-213.1971
更新日期:1971-02-01 00:00:00
abstract::The influenza A virus (IAV) M2 protein is a multifunctional protein with critical roles in virion entry, assembly, and budding. M2 is targeted to the apical plasma membrane of polarized epithelial cells, and the interaction of the viral proteins M2, M1, HA, and NA near glycolipid rafts in the apical plasma membrane is...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01425-18
更新日期:2018-10-29 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.8.6888-6892.1998
更新日期:1998-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.19.9068-9076.2001
更新日期:2001-10-01 00:00:00
abstract:UNLABELLED:Transmission of chronic wasting disease (CWD) between cervids is influenced by the primary structure of the host cellular prion protein (PrP(C)). In white-tailed deer, PRNP alleles encode the polymorphisms Q95 G96 (wild type [wt]), Q95 S96 (referred to as the S96 allele), and H95 G96 (referred to as the H95 ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02010-15
更新日期:2015-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.45.3.1147-1149.1983
更新日期:1983-03-01 00:00:00
abstract::Cervical cancer develops through the combined activities of the human papillomavirus (HPV) E6 and E7 oncoproteins. A defining characteristic of E6 oncoproteins derived from cancer-causing HPV types is the presence of a PDZ binding motif (PBM) at the extreme carboxy terminus of the protein which is absent from E6 prote...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02074-12
更新日期:2013-02-01 00:00:00
abstract::Herpes simplex virus type 1 (HSV-1) infection causes the active degradation of the catalytic subunit of DNA-dependent protein kinase (DNA-PKcs), and this process is reliant on the expression of the HSV-1 immediate-early protein Vmw110. In this study we investigated in more detail the mechanism by which the degradation...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.1.650-657.1999
更新日期:1999-01-01 00:00:00
abstract::The region of the human cytomegalovirus (CMV) genome between the UL127 open reading frame and the major immediate-early (MIE) enhancer is referred to as the unique region. DNase I protection analysis with human cell nuclear extracts demonstrated multiple protein binding sites in this region of the viral genome (P. Gha...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.11.9039-9052.1999
更新日期:1999-11-01 00:00:00
abstract::From the results of transient plasmid-based replication assays, it has been postulated that homologous regions (hrs) of Autographa californica nuclear polyhedrosis virus (AcMNPV) function as origins of viral DNA replication. However, these assays vary in specificity according to the methodology used and may not be dep...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.10.6967-6972.1996
更新日期:1996-10-01 00:00:00
abstract::The hepatitis C virus (HCV) encodes a large polyprotein; therefore, all viral proteins are produced in equimolar amounts regardless of their function. The aim of our study was to determine the ratio of nonstructural proteins to RNA that is required for HCV RNA replication. We analyzed Huh-7 cells harboring full-length...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.21.13594-13605.2005
更新日期:2005-11-01 00:00:00
abstract::The DNA sequence of 3,240 nucleotides of the XbaI G fragment located in the unique long (UL) region of the equine herpesvirus 1 genome revealed two major open reading frames (ORFs) designated UL3 and UL4. The UL3 ORF of 470 amino acids (aa) maps at nucleotides (nt) 4450 to 3038 from the long terminus, and its predicte...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.9.5363-5372.1992
更新日期:1992-09-01 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) uses a variety of chemokine receptors as coreceptors for virus entry, and the ability of the virus to be neutralized by antibody may depend on which coreceptors are used. In particular, laboratory-adapted variants of the virus that use CXCR4 as a coreceptor are highly sensit...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.3.1886-1893.1998
更新日期:1998-03-01 00:00:00
abstract::We are exploring the use of interferon (IFN) genes for somatic cell gene therapy and have investigated the possibility of transforming cells into low constitutive IFN producers over prolonged periods of time without impeding cell survival or replication. Here we report the possibility of conferring a permanent antivir...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.2.664-671.1991
更新日期:1991-02-01 00:00:00