Poliovirus requires a precise 5' end for efficient positive-strand RNA synthesis.

Abstract:

:Poliovirus infectious RNA can be synthesized in vitro using phage DNA-dependent RNA-polymerases. These synthetic transcripts contain several extra nucleotides at the 5' end, which are deleted during replication to generate authentic viral genomes. We removed those 5'-end extra nucleotides utilizing a hammerhead ribozyme to produce transcripts with accurate 5' ends. These transcripts replicate substantially more rapidly in cell culture, demonstrating no lag before replication; they also replicate more efficiently in Xenopus laevis oocytes and in in vitro translation-replication cell extracts. In both systems, an exact 5' end is necessary for synthesis of positive-strand RNA but not negative-strand RNA.

journal_name

J Virol

journal_title

Journal of virology

authors

Herold J,Andino R

doi

10.1128/jvi.74.14.6394-6400.2000

subject

Has Abstract

pub_date

2000-07-01 00:00:00

pages

6394-400

issue

14

eissn

0022-538X

issn

1098-5514

journal_volume

74

pub_type

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