Abstract:
:Poliovirus infectious RNA can be synthesized in vitro using phage DNA-dependent RNA-polymerases. These synthetic transcripts contain several extra nucleotides at the 5' end, which are deleted during replication to generate authentic viral genomes. We removed those 5'-end extra nucleotides utilizing a hammerhead ribozyme to produce transcripts with accurate 5' ends. These transcripts replicate substantially more rapidly in cell culture, demonstrating no lag before replication; they also replicate more efficiently in Xenopus laevis oocytes and in in vitro translation-replication cell extracts. In both systems, an exact 5' end is necessary for synthesis of positive-strand RNA but not negative-strand RNA.
journal_name
J Viroljournal_title
Journal of virologyauthors
Herold J,Andino Rdoi
10.1128/jvi.74.14.6394-6400.2000subject
Has Abstractpub_date
2000-07-01 00:00:00pages
6394-400issue
14eissn
0022-538Xissn
1098-5514journal_volume
74pub_type
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