Abstract:
:Bluetongue virus (BTV), an insect-vectored emerging pathogen of both wild ruminants and livestock, has had a severe economic impact in agriculture in many parts of the world. The investigation of BTV replication and pathogenesis has been hampered by the lack of a reverse genetics system. Recovery of infectious BTV is possible by the transfection of permissive cells with the complete set of 10 purified viral mRNAs derived in vitro from transcribing cores (M. Boyce and P. Roy, J. Virol. 81:2179-2186, 2007). Here, we report that in vitro synthesized T7 transcripts, derived from cDNA clones, can be introduced into the genome of BTV using a mixture of T7 transcripts and core-derived mRNAs. The replacement of genome segment 10 and the simultaneous replacement of segments 2 and 5 encoding the two immunologically important outer capsid proteins, VP2 and VP5, are described. Further, we demonstrate the recovery of infectious BTV entirely from T7 transcripts, proving that synthetic transcripts synthesized in the presence of cap analogue can functionally substitute for viral transcripts at all stages of the BTV replication cycle. The generation of BTV with a fully defined genome permits the recovery of mutations in a defined genetic background. The ability to generate specific mutants provides a new tool to investigate the BTV replication cycle as well as permitting the generation of designer vaccine strains, which are greatly needed in many countries.
journal_name
J Viroljournal_title
Journal of virologyauthors
Boyce M,Celma CC,Roy Pdoi
10.1128/JVI.00808-08subject
Has Abstractpub_date
2008-09-01 00:00:00pages
8339-48issue
17eissn
0022-538Xissn
1098-5514pii
JVI.00808-08journal_volume
82pub_type
杂志文章abstract::A virus (M-7) isolated from baboon placental tissue demonstrates many similarities to endogenous feline virus RD-114. Immunodiffusion analysis shows a group-specific antigen (gs-1) line of identity between M-7 and RD-114. Anti-RD-114 DNA polymerase IgG inhibits M-7 polymerase by 57% compared to 97% for RD-114. M-7 vir...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.14.1.133-138.1974
更新日期:1974-07-01 00:00:00
abstract::The vif gene of human immunodeficiency virus type 1 is absolutely required for productive infection of primary cells derived from human blood and certain immortalized T lymphoid cells, for example, H9. Cells with this restrictive phenotype are termed nonpermissive, whereas cell lines in which vif-deficient virus can r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.8.5297-5305.1996
更新日期:1996-08-01 00:00:00
abstract::Herpes simplex virus type 1 (HSV-1) infection causes the active degradation of the catalytic subunit of DNA-dependent protein kinase (DNA-PKcs), and this process is reliant on the expression of the HSV-1 immediate-early protein Vmw110. In this study we investigated in more detail the mechanism by which the degradation...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.1.650-657.1999
更新日期:1999-01-01 00:00:00
abstract::Kaposi's sarcoma-associated herpesvirus (KSHV) utilizes clathrin-mediated endocytosis for its infectious entry into human foreskin fibroblast (HFF) cells (S. M. Akula, P. P. Naranatt, N.-S. Walia, F.-Z. Wang, B. Fegley, and B. Chandran, J. Virol. 77:7978-7990, 2003). Here, we characterized KSHV entry into primary huma...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02498-08
更新日期:2009-05-01 00:00:00
abstract::The reovirus core particle is a molecular machine that mediates synthesis, capping, and export of the viral plus strand RNA transcripts. Its assembly and structure-function relationships remain to be well understood. Following the lead of previous studies with other Reoviridae family members, most notably orbiviruses ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.23.12211-12222.2002
更新日期:2002-12-01 00:00:00
abstract::Infection of primary fibroblasts with human cytomegalovirus (HCMV) causes a rapid stabilization of the cellular protein p53. p53 is a major effector of the cellular damage response, and activation of this transcription factor can lead either to cell cycle arrest or to apoptosis. Viruses employ many tactics to avoid p5...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00505-06
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01581-07
更新日期:2008-02-01 00:00:00
abstract::Mutants (N(-)nin) of bacteriophage lambda in which the N gene product is not required for growth on wild-type Escherichia coli do not plate on recA bacterial mutants. Secondary mutants, selected for growth on recA, lie within the immunity region to the right of gene cI and appear identical to the cro mutants of Eisen ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.9.6.938-945.1972
更新日期:1972-06-01 00:00:00
abstract::Hepatitis E virus genotype 1 strain Sar55 replicated in subcloned Caco-2 intestinal cells and Huh7 hepatoma cells that had been transfected with in vitro transcribed viral genomes, and hepatitis E virions were released into the culture medium of both cell lines. Virus egress from cells depended on open reading frame 3...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00593-10
更新日期:2010-09-01 00:00:00
abstract::A vaccine regimen that elicits broadly neutralizing antibodies (bNAbs) is a major goal in HIV-1 vaccine research. In this study, we assessed the immunogenicity of the CAP256 superinfecting viral envelope (CAP256 SU) protein delivered by modified vaccinia virus Ankara (MVA) and DNA vaccines in different prime-boost com...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02155-18
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abstract::Recently we reported that in vitro RNA transcripts complementary to the genome of hepatitis delta virus (HDV) contain a unique site at which self-cleavage can occur. Subsequent studies showed that a similar self-cleavage site was present on in vitro RNA transcripts of genomic HDV RNA. The same self-cleavage reactions ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.12.4439-4444.1988
更新日期:1988-12-01 00:00:00
abstract::Cytotoxic T lymphocytes (CTL) play an important role in recovery from a number of viral infections. They are also implicated in virus-induced immunopathology as best demonstrated in lymphocytic choriomeningitis virus (LCMV) infection of adult immunocompetent mice. In the present study, the structure of the T-cell rece...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.12.5919-5926.1990
更新日期:1990-12-01 00:00:00
abstract::We report sequence hypervariability in the viral protein 1 (VP1) interaction domain of VP2 in the norovirus (NoV) genogroup II genotype 4 (GII.4) lineage on 3 levels: (i) the global evolution of pandemic/epidemic strains from the mid-1970s through post-2006, (ii) the local emergence of an epidemic strain, and (iii) an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00228-11
更新日期:2012-01-01 00:00:00
abstract::The emergence in 1997 and continuance today of a highly lethal H5N1 avian influenza virus (AIV) causing human disease has raised concern about an impending pandemic and the need for a vaccine to prepare for such an occurrence. We previously generated an efficacious vesicular stomatitis virus (VSV)-based AIV vaccine ex...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02637-09
更新日期:2010-05-01 00:00:00
abstract::The feline immunodeficiency virus (FIV) cat model is extensively used to investigate possible vaccination approaches against AIDS in humans. Although consistent levels of protection have been achieved with FIV, as with other model systems, by immunizing with whole inactivated virus or fixed infected cells, the mechani...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.1.1-10.1999
更新日期:1999-01-01 00:00:00
abstract::Porcine reproductive and respiratory syndrome (PRRS) virus (PRRSV), a positive-strand RNA virus that belongs to the Arteriviridae family of Nidovirales, has been identified as the causative agent of PRRS. Nsp1alpha is the amino (N)-terminal protein in a polyprotein encoded by the PRRSV genome and is reported to be cru...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02579-08
更新日期:2009-11-01 00:00:00
abstract::We have previously shown that styrylquinolines (SQLs) are integrase inhibitors in vitro. They compete with the long terminal repeat substrate for integrase. Here, we describe the cellular mode of action of these molecules. We show that SQLs do not interfere with virus entry. In fact, concentrations of up to 20 times t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.11.5728-5736.2004
更新日期:2004-06-01 00:00:00
abstract::The nef gene contributes to the replication of primate lentiviruses by altering the trafficking of cellular proteins involved in adaptive immunity (class I and II major histocompatibility complex [MHC]) and viral transmission (CD4 and DC-SIGN). A conserved acidic leucine-based sequence (E(160)xxxLL) within human immun...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.4.1837-1849.2006
更新日期:2006-02-01 00:00:00
abstract::The morphology and development of four members of the reticuloendotheliosis virus group were studied by transmission electron microscopy. Virions of duck spleen necrosis virus, duck infectious anemia virus, chicken syncytial virus, and reticuloendotheliosis virus strain T are sperical with a diameter of approximately ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.16.4.1027-1038.1975
更新日期:1975-10-01 00:00:00
abstract::The parvovirus minute virus of mice (MVM) packages predominantly negative-sense single strands, while its close relative LuIII encapsidates strands of both polarities with equal efficiency. Using genomic chimeras and mutagenesis, we show that the ability to package positive strands maps not, as originally postulated, ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.4.2287-2300.2005
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abstract::Replication by many positive-strand RNA viruses includes genomic RNA amplification and subgenomic mRNA (sgRNA) transcription. For brome mosaic virus (BMV), both processes occur in virus-induced, membrane-associated compartments, require BMV replication factors 1a and 2a, and use negative-strand RNA3 as a template for ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.3.1438-1451.2005
更新日期:2005-02-01 00:00:00
abstract::In 1997, an H5N1 influenza virus outbreak occurred in chickens in Hong Kong, and the virus was transmitted directly to humans. Because there is limited information about the avian influenza virus reservoir in that region, we genetically characterized virus strains isolated in Hong Kong during the 1997 outbreak. We seq...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.14.6309-6315.2000
更新日期:2000-07-01 00:00:00
abstract::The kinetics of retroviral DNA and RNA synthesis are parameters vital to understanding viral growth, especially for human immunodeficiency virus (HIV), which encodes several of its own regulatory genes. We have established a single-cycle growth condition for HIV in H9 cells, a human CD4+ lymphocyte line. The full-leng...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.9.3708-3713.1989
更新日期:1989-09-01 00:00:00
abstract::Picornavirus-infected cells contain a double-stranded RNA (replicative form [RF] RNA), composed of viral genomic RNA hydrogen bonded to cRNA of similar nucleotide length. Mengovirus RF RNA reacted with a succinimide ester of biotin was shown by electron microscopy to bind avidin-coupled polymethacrylate spheres. These...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.39.1.229-237.1981
更新日期:1981-07-01 00:00:00
abstract::We have been investigating the first viral genes to be transcribed in Spodoptera frugiperda cells after the infection with Autographa california nuclear polyhedrosis virus in order to identify regulatory proteins required for the activation of early and/or late gene transcription. By screening a cDNA library from poly...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.2.753-758.1993
更新日期:1993-02-01 00:00:00
abstract::2'-5'-Oligoadenylate synthetase-like protein (OASL) is an interferon-inducible antiviral protein. Here we describe differential inhibitory activities of human OASL and the two mouse OASL homologs against respiratory syncytial virus (RSV) replication. Interestingly, nonstructural protein 1 (NS1) of RSV promoted proteas...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01076-15
更新日期:2015-10-01 00:00:00
abstract::Recombinant viruses possessing reporter proteins have been generated for virus research. In the case of the family Flaviviridae, we recently generated recombinant viruses, including the hepatitis C virus of the genus Hepacivirus, Japanese encephalitis virus (JEV) of the genus Flavivirus, and bovine viral diarrhea viru...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01191-19
更新日期:2019-10-29 00:00:00
abstract::Expression of the human T-cell leukemia virus type 1 (HTLV-1) oncoprotein Tax is correlated with cellular transformation contributing to the development of adult T-cell leukemia. Tax has been shown to modulate the activities of several cellular promoters. Existing evidence suggests that Tax need not directly bind to D...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.5.2161-2173.2001
更新日期:2001-03-01 00:00:00
abstract::Residual hepatitis B virus (HBV) DNA can be detected in serum and liver after apparent recovery from transient infection. However, it is not known if this residual HBV DNA represents ongoing viral replication and antigen expression. In the current study, ducks inoculated with duck hepatitis B virus (DHBV) were monitor...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.19.12242-12252.2005
更新日期:2005-10-01 00:00:00
abstract::The EFII cis element is a 38-bp sequence at the 5' end of the Rous sarcoma virus long terminal repeat, extending from nucleotides -229 to -192 (with respect to the viral transcription start site), which is recognized by sequence-specific DNA-binding proteins in avian fibroblast nuclear extracts (L. Sealy and R. Chalkl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.11.6338-6352.1992
更新日期:1992-11-01 00:00:00