Abstract:
:Envelope glycoprotein E(rns) of classical swine fever virus (CSFV) has been shown to contain RNase activity and is involved in virus infection. Two short regions of amino acids in the sequence of E(rns) are responsible for RNase activity. In both regions, histidine residues appear to be essential for catalysis. They were replaced by lysine residues to inactivate the RNase activity. The mutated sequence of E(rns) was inserted into the p10 locus of a baculovirus vector and expressed in insect cells. Compared to intact E(rns), the mutated proteins had lost their RNase activity. The mutated proteins reacted with E(rns)-specific neutralizing monoclonal and polyclonal antibodies and were still able to inhibit infection of swine kidney cells (SK6) with CSFV, but at a concentration higher than that measured for intact E(rns). This result indicated that the conformation of the mutated proteins was not severely affected by the inactivation. To study the effect of these mutations on virus infection and replication, a CSFV mutant with an inactivated E(rns) (FLc13) was generated with an infectious DNA copy of CSFV strain C. The mutant virus showed the same growth kinetics as the parent virus in cell culture. However, in contrast to the parent virus, the RNase-negative virus induced a cytopathic effect in swine kidney cells. This effect could be neutralized by rescue of the inactivated E(rns) gene and by neutralizing polyclonal antibodies directed against E(rns), indicating that this effect was an inherent property of the RNase-negative virus. Analyses of cellular DNA of swine kidney cells showed that the RNase-negative CSFV induced apoptosis. We conclude that the RNase activity of envelope protein E(rns) plays an important role in the replication of pestiviruses and speculate that this RNase activity might be responsible for the persistence of these viruses in their natural host.
journal_name
J Viroljournal_title
Journal of virologyauthors
Hulst MM,Panoto FE,Hoekman A,van Gennip HG,Moormann RJdoi
10.1128/JVI.72.1.151-157.1998subject
Has Abstractpub_date
1998-01-01 00:00:00pages
151-7issue
1eissn
0022-538Xissn
1098-5514journal_volume
72pub_type
杂志文章abstract::The tempo and intensity of retroviral neuropathogenesis are dependent on the capacity of the virus to invade the central nervous system. For murine leukemia viruses, an important determinant of neuroinvasiveness is the virus-encoded protein glycosylated Gag, the function of which in the virus life cycle is not known. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.7.5619-5625.1998
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doi:10.1128/JVI.63.5.1981-1988.1989
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pub_type: 杂志文章
doi:10.1128/JVI.67.6.3304-3311.1993
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journal_title:Journal of virology
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doi:10.1128/jvi.74.7.3330-3337.2000
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02692-10
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.5.3050-3057.2003
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journal_title:Journal of virology
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pub_type: 杂志文章
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更新日期:1968-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.31.3.621-629.1979
更新日期:1979-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.44.3.1056-1067.1982
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pub_type: 杂志文章
doi:10.1128/JVI.02681-12
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pub_type: 杂志文章
doi:10.1128/JVI.63.2.730-738.1989
更新日期:1989-02-01 00:00:00
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journal_title:Journal of virology
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doi:10.1128/JVI.9.2.263-272.1972
更新日期:1972-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jvi.78.10.5288-5298.2004
更新日期:2004-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
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journal_title:Journal of virology
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pub_type: 杂志文章
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更新日期:1984-05-01 00:00:00
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journal_title:Journal of virology
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更新日期:2004-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Journal of virology
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1973-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1994-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.12.6705-6713.1991
更新日期:1991-12-01 00:00:00