Efficient replication of human immunodeficiency virus type 1 requires a threshold level of Rev: potential implications for latency.

Abstract:

:The Rev protein of human immunodeficiency virus type 1 (HIV-1) is essential for the expression of the structural genes of HIV-1. To determine whether a functional threshold level of Rev is required to allow efficient HIV-1 replication, CD4-positive HeLa cells, constitutively expressing a Rev-deficient provirus, were transfected with various quantities of a Rev-expressing plasmid. Compared with the quantity of the Rev-producing plasmid transfected, HIV-1 replication was distinctly nonlinear as measured by HIV-1 p24 antigen and HIV-1-specific RNA production. A quantitative RNA polymerase chain reaction (PCR) demonstrated that Rev mRNA expression was linearly correlated with the quantity of Rev-expressing plasmid which was transfected into these cells. These data suggest that a critical threshold of Rev is required for a highly productive HIV-1 infection. This threshold level of Rev may be involved in the generation and maintenance of HIV-1 proviral latency.

journal_name

J Virol

journal_title

Journal of virology

authors

Pomerantz RJ,Seshamma T,Trono D

doi

10.1128/JVI.66.3.1809-1813.1992

subject

Has Abstract

pub_date

1992-03-01 00:00:00

pages

1809-13

issue

3

eissn

0022-538X

issn

1098-5514

journal_volume

66

pub_type

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