Abstract:
:Heating oncornavirus RNAs at temperatures insufficient for complete denaturation results in forms migrating between the native form (vRNA) and the completely denatured form (vRNA) after gel electrophoresis. Intermediate forms from Rous sarcoma virus or murine leukemia virus were isolated after heating of vRNA's at 58 degrees C and sedimenting in sucrose gradients, and at least four intermediates could be identified in each case. Melting of feline virus (RD-114) RNA produced one major intermediate which required a comparatively high temperature to denature, and a second intermediate occurring in conditions of low ionic strength. Although the subunit model for oncornavirus RNA is not excluded by these data, we propose that vRNA, vRNA', and intermediates may be configurational variants of the same molecule, and a monomer model for oncornavirus RNA is presented.
journal_name
J Viroljournal_title
Journal of virologyauthors
Bader JP,Ray DAdoi
10.1128/JVI.19.3.810-819.1976subject
Has Abstractpub_date
1976-09-01 00:00:00pages
810-9issue
3eissn
0022-538Xissn
1098-5514journal_volume
19pub_type
杂志文章abstract:UNLABELLED:T-cell immunoglobulin and mucin domain 1 (TIM-1) and other TIM family members were recently identified as phosphatidylserine (PtdSer)-mediated virus entry-enhancing receptors (PVEERs). These proteins enhance entry of Ebola virus (EBOV) and other viruses by binding PtdSer on the viral envelope, concentrating ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00300-14
更新日期:2014-06-01 00:00:00
abstract::Three fish retroviruses infecting walleyes constitute the recently recognized genus called epsilonretrovirus. The founding member of this group, walleye dermal sarcoma virus (WDSV), induces benign skin tumors in the infected fish and replicates near 4 degrees C. While the viral genomic sequence is known, biochemical c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.9.4341-4349.2002
更新日期:2002-05-01 00:00:00
abstract::The coronavirus nucleocapsid (N) protein plays a multifunctional role in the virus life cycle, from regulation of replication and transcription and genome packaging to modulation of host cell processes. These functions are likely to be facilitated by interactions with host cell proteins. The potential interactome of t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00321-13
更新日期:2013-09-01 00:00:00
abstract::The first morphological evidence of African swine fever virus (ASFV) assembly is the appearance of precursor viral membranes, thought to derive from the endoplasmic reticulum, within the assembly sites. We have shown previously that protein p54, a viral structural integral membrane protein, is essential for the genera...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00600-10
更新日期:2010-08-01 00:00:00
abstract::Although xenotropic murine leukemia viruses cannot productively infect cells of laboratory mice, cells from various wild-derived mice can support replication of these viruses. Although the virus-sensitive wild mice generally lack all or most of the xenotropic proviral genes characteristic of inbred strains, susceptibi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.55.3.690-695.1985
更新日期:1985-09-01 00:00:00
abstract::Latent Epstein-Barr virus (EBV) infection is associated with several lymphoproliferative disorders, including posttransplant lymphoma, Hodgkin's disease, and Burkitt's lymphoma, as well as nasopharyngeal carcinoma (NPC). Twenty-nine microRNAs (miRNAs) have been identified that are transcribed during latent infection f...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00785-08
更新日期:2008-09-01 00:00:00
abstract::We have identified a trans-dominant mutant form of the herpes simplex virus (HSV) DNA-binding protein ICP8 which inhibits viral replication. When expressed by the V2.6 cell line, the mutant gene product inhibited wild-type HSV production by 50- to 150-fold when the multiplicity of infection was less than 5. Production...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.5.2666-2675.1991
更新日期:1991-05-01 00:00:00
abstract::H9N2 influenza viruses are panzootic in domestic poultry in Eurasia and since 1999 have caused transient infections in humans and pigs. To investigate the zoonotic potential of H9N2 viruses, we studied the evolution of the viruses in live-poultry markets in Hong Kong in 2003. H9N2 was the most prevalent influenza viru...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.16.8609-8614.2004
更新日期:2004-08-01 00:00:00
abstract::We characterized human immunodeficiency virus type 1 (HIV-1) envelope glycoproteins (Env) isolated from two HIV-1-infected CCR5delta32 homozygotes. Envs from both subjects used CCR5 and CXCR4 for entry into transfected cells. Most R5X4 Envs were lymphocyte-tropic and used CXCR4 exclusively for entry into peripheral bl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.7.3684-3691.2006
更新日期:2006-04-01 00:00:00
abstract::The baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV), the type species of alphabaculoviruses, is an enveloped DNA virus that infects lepidopteran insects and is commonly known as a vector for protein expression and cell transduction. AcMNPV belongs to a diverse group of viral and bacterial pat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00111-18
更新日期:2018-05-14 00:00:00
abstract::A ribonucleic acid (RNA)-dependent RNA polymerase was induced in chick embryo fibroblast cells after infection with Sendai virus (parainfluenza 1 virus). The enzyme was associated with the microsomal fraction of infected cells and reached maximum detectable activity at 18 hr after virus infection. The activity of the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.5.6.663-671.1970
更新日期:1970-06-01 00:00:00
abstract::We molecularly cloned JC polyomavirus DNAs from urine samples of eight nonimmunosuppressed patients and two healthy individuals. The cloned viral DNAs all contained an archetypal regulatory sequence from which various regulatory sequences of JC polyomavirus isolates derived from patients with progressive multifocal le...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.6.3139-3143.1990
更新日期:1990-06-01 00:00:00
abstract:UNLABELLED:Gammaherpesviruses establish persistent, systemic infections and cause cancers. Murid herpesvirus 4 (MuHV-4) provides a unique window into the early events of host colonization. It spreads via lymph nodes. While dendritic cells (DC) pass MuHV-4 to lymph node B cells, subcapsular sinus macrophages (SSM), whic...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01108-16
更新日期:2016-09-29 00:00:00
abstract::Human group C adenoviruses cause an acute infection in respiratory epithelia and establish a long-term or persistent infection, possibly in lymphocytes. The mechanism by which this persistence is maintained is unknown; however, it would require that persistently infected lymphocytes not be deleted. The adenovirus geno...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.19.9716-9723.2002
更新日期:2002-10-01 00:00:00
abstract::Autophagy is an intracellular degradation pathway that provides a host defense mechanism against intracellular pathogens. However, many viruses exploit this mechanism to promote their replication. This study shows that lytic induction of Epstein-Barr virus (EBV) increases the membrane-bound form of LC3 (LC3-II) and LC...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02033-14
更新日期:2014-10-01 00:00:00
abstract::The interaction of the mouse hepatitis virus (MHV) nucleocapsid protein (N) and viral RNA was examined. Monoclonal antibody specific for N protein coimmunoprecipitated MHV genomic RNA as well as all six MHV subgenomic mRNAs found in MHV-infected cells. In contrast, monoclonal antibodies to the MHV E2 or E1 envelope gl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.11.4280-4287.1988
更新日期:1988-11-01 00:00:00
abstract::More often than not, analyses of virus evolution have considered that virus populations are so large that evolution can be explained by purely deterministic models. However, virus populations could have much smaller effective numbers than the huge reported census numbers, and random genetic drift could be important in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.18.9906-9911.2003
更新日期:2003-09-01 00:00:00
abstract::The Group B Staphylococcus phage P11-M15 is shown to be 51% protein and 49% deoxyribonucleic acid (DNA). The intact virion has a molecular weight of 66.7 x 10(6) daltons. The purified viral DNA has a molecular weight of 32.7 x 10(6) daltons. The intact virion is shown to be composed of a polyhedral head which is attac...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.9.4.664-671.1972
更新日期:1972-04-01 00:00:00
abstract::The Epstein-Barr virus (EBV) lytic activator genes bzlf1 and brlf1 are conventionally referred to as immediate-early (IE) genes. However, previous studies showed that the earliest expression of these genes was blocked by cycloheximide when the EBV lytic cycle was induced by histone deacetylase (HDAC) inhibitors and pr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01415-10
更新日期:2010-12-01 00:00:00
abstract::The role of the 5' nontranslated region in the replication of hepatitis A virus (HAV) was studied by analyzing the translation and replication of chimeric RNAs containing the encephalomyocarditis virus (EMCV) internal ribosome entry segment (IRES) and various lengths (237, 151, or 98 nucleotides [nt]) of the 5'-termin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.5.2861-2868.1996
更新日期:1996-05-01 00:00:00
abstract::Many viruses express inhibitors of programmed cell death (apoptosis), thereby countering host defenses that would otherwise rapidly clear infected cells. To counter this, viruses such as adenoviruses and herpesviruses express recognizable homologs of the mammalian prosurvival protein Bcl-2. In contrast, the majority o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01115-12
更新日期:2012-11-01 00:00:00
abstract::The pandemic H1N1 virus of 2009 (2009 H1N1) continues to cause illness worldwide, primarily in younger age groups. To better understand the pathogenesis of these viruses in mammals, we used a mouse model to evaluate the relative virulence of selected 2009 H1N1 viruses and compared them to a representative human triple...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02742-09
更新日期:2010-05-01 00:00:00
abstract::The Parvovirus H-1 replicates autonomously in hamster embryo cells. A DNA synthetic event, called HA-DNA synthesis, upon which subsequent viral RNA and viral hemagglutinin synthesis is dependent, is initiated in late S phase of the infected cell (18). It was postulated that HA-DNA represents parental viral replicative...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.2.400-410.1974
更新日期:1974-02-01 00:00:00
abstract::Rhesus lymphocryptovirus (rLCV) and Epstein-Barr virus (EBV) are closely related gammaherpesviruses that infect and cause disease in rhesus monkeys and humans, respectively. Thus, rLCV is an important model system for EBV pathogenesis. Both rLCV and EBV express microRNAs (miRNAs), several conserved in sequence and gen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00110-10
更新日期:2010-05-01 00:00:00
abstract::Mutagenesis of the transmembrane domain and cytoplasmic tail of human immunodeficiency virus type 1 envelope glycoprotein gp160 revealed that its intracellular transport and processing in transfected cell lines were modulated by a functional domain included in the carboxy-terminal sequence consisting of residues 751 t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.6.3100-3103.1990
更新日期:1990-06-01 00:00:00
abstract::A number of positive-strand RNA viruses, such as hepatitis C virus (HCV) and poliovirus, use double-membrane vesicles (DMVs) as replication sites. However, the role of cellular proteins in DMV formation during virus replication is poorly understood. HCV NS4B protein induces the formation of a "membranous web" structur...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00858-19
更新日期:2020-01-06 00:00:00
abstract::In this study, we have identified an internal ribosome entry site (IRES) from the highly infectious herpesvirus Marek's disease virus (MDV). The IRES was mapped to the intercistronic region (ICR) of a bicistronic mRNA that we cloned from the MDV-transformed CD4(+) T-cell line MSB-1. The transcript is a member of a fam...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02602-08
更新日期:2009-06-01 00:00:00
abstract::In less than 3 months after the first cases of swine origin 2009 influenza A (H1N1) virus infections were reported from Mexico, WHO declared a pandemic. The pandemic virus is antigenically distinct from seasonal influenza viruses, and the majority of human population lacks immunity against this virus. We have studied ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01619-09
更新日期:2010-02-01 00:00:00
abstract::The African swine fever virus protein A238L inhibits activation of NFAT transcription factor by binding calcineurin and inhibiting its phosphatase activity. NFAT controls the expression of many immunomodulatory proteins. Here we describe a 14-amino-acid region of A238L that is needed and sufficient for binding to calc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.20.9412-9420.2000
更新日期:2000-10-01 00:00:00
abstract:UNLABELLED:Cell culture (cc)-derived hepatitis B virus (HBV) can infect differentiated HepaRG cells, but efficient infection requires addition of polyethylene glycol (PEG) during inoculation. Identification of sodium taurocholate cotransporting polypeptide (NTCP) as an HBV receptor enabled ccHBV infection of NTCP recon...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01153-16
更新日期:2016-08-26 00:00:00