Abstract:
:The interaction of the mouse hepatitis virus (MHV) nucleocapsid protein (N) and viral RNA was examined. Monoclonal antibody specific for N protein coimmunoprecipitated MHV genomic RNA as well as all six MHV subgenomic mRNAs found in MHV-infected cells. In contrast, monoclonal antibodies to the MHV E2 or E1 envelope glycoproteins, an anti-I-A monoclonal antibody, and serum samples from lupus patients did not immunoprecipitate the MHV mRNAs. Moreover, the anti-N monoclonal antibody did not coimmunoprecipitate vesicular stomatitis virus RNA or host cell RNA under conditions which immunoprecipitated all MHV RNAs. These data suggest a specific interaction between the N protein and the virus-specific mRNAs. Both the membrane-bound and cytosolic small MHV leader-specific RNAs of greater than 65 nucleotides long were immunoprecipitated only by anti-N monoclonal antibody. These data suggest that an N binding site is present within the leader RNA sequences at a site at least 65 nucleotides from the 5' end of genomic RNA and all six subgenomic mRNAs. The larger leader-containing RNAs originating from mRNA 1 and mRNA 6, as well as the MHV negative-stranded RNA, were also immunoprecipitated by the anti-N monoclonal antibody. These data indicate that the MHV N protein is associated with MHV-specific RNAs and RNA intermediates and may play an important functional role during MHV transcription and replication.
journal_name
J Viroljournal_title
Journal of virologyauthors
Baric RS,Nelson GW,Fleming JO,Deans RJ,Keck JG,Casteel N,Stohlman SAdoi
10.1128/JVI.62.11.4280-4287.1988subject
Has Abstractpub_date
1988-11-01 00:00:00pages
4280-7issue
11eissn
0022-538Xissn
1098-5514journal_volume
62pub_type
杂志文章abstract::Epstein-Barr virus (EBV) is associated with about 6 to 16% of gastric carcinoma cases worldwide. Expression of the EBV microRNAs (miRNAs) was observed in B cells and nasopharyngeal carcinoma cells infected with EBV. However, it is not clear if the EBV miRNAs are expressed in EBV-associated gastric carcinomas (EBVaGCs)...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02271-06
更新日期:2007-01-01 00:00:00
abstract::Paramyxoviruses such as human parainfluenza viruses that bear inserts encoding protective antigens of heterologous viruses can induce an effective immunity against the heterologous viruses in experimental animals. However, vectors based on common human pathogens would be expected to be restricted in replication in the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.21.13275-13284.2005
更新日期:2005-11-01 00:00:00
abstract::Transfection has been found and characterized in pneumococcus. For replicating omega3 phage DNA extracted from infected cells, transfection was relatively efficient and rose linearly with DNA concentration and quadratically with time, according to T(T - 3.5) min(2). For mature DNA extracted from phage particles, trans...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.25.1.60-72.1978
更新日期:1978-01-01 00:00:00
abstract::In order to elucidate the molecular mechanisms of action of the tat-responsive sequence, mutational analysis of the tat-responsive sequence was carried out. The most critical region comprised nucleotides +18 to +44 and included the 3-nucleotide bulge at positions +23 to +25, the loop sequence, and an intact stem. In a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.3.1402-1406.1990
更新日期:1990-03-01 00:00:00
abstract::Nucleotide sequences were determined for the complete M genome segments of two distinct hantavirus genetic lineages which were detected in hantavirus antibody- and PCR-positive white-footed mice (Peromyscus leucopus) from Indiana and Oklahoma. Phylogenetic analyses indicated that although divergent from each other, th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.1.57-64.1998
更新日期:1998-01-01 00:00:00
abstract::A receptor on YAC-1 cells, a mouse T-lymphoma cell line, bound all six serotypes of the group B coxsackieviruses (CVB). In addition, the cells produced infectious virus. Each of the CVB competed for the same receptor on YAC-1 cells. CVB3 bound relatively slowly to YAC-1 cells (k = 4 x 10(-11) min-1 cell-1), and there ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.7.3105-3108.1989
更新日期:1989-07-01 00:00:00
abstract::Poliovirus infectious RNA can be synthesized in vitro using phage DNA-dependent RNA-polymerases. These synthetic transcripts contain several extra nucleotides at the 5' end, which are deleted during replication to generate authentic viral genomes. We removed those 5'-end extra nucleotides utilizing a hammerhead ribozy...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.14.6394-6400.2000
更新日期:2000-07-01 00:00:00
abstract::Historically, the adenoviral E3 region was found to be nonessential for viral replication in vitro. In addition, adenoviruses whose genome was more than approximately 105% the size of the native genome were inefficiently packaged. These profound observations were used experimentally to insert transgenes into the adeno...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.9.5455-5465.2005
更新日期:2005-05-01 00:00:00
abstract::Processing of the hepatitis C virus polyprotein is accomplished by a series of cotranslational and posttranslational cleavages mediated by host cell signalases and two virally encoded proteinases. Of these the NS3 proteinase is essential for processing at the NS3/4A, NS4A/4B, NS4B/5A, and NS5A/5B junctions. Processing...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.1.198-205.1995
更新日期:1995-01-01 00:00:00
abstract::The structural gene for diphtherial toxin is present in corynebacteriophage beta. Previous studies located several point mutations within the tox locus and determined the orientation of transcription of the toxin structural gene. The production of maximal quantities of toxin by Corynebacterium diphtheriae C7(beta) occ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.37.3.946-954.1981
更新日期:1981-03-01 00:00:00
abstract::The expression strategy of parvoviruses of the Densovirus genus has as yet not been reported. Clones were obtained from the densonucleosis virus of Galleria mellonella (GmDNV) that yielded infectious virus upon transfection into LD652 cells. Its genome was found to be the longest (6,039 nucleotides [nt]), with the lar...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.19.10357-10365.2003
更新日期:2003-10-01 00:00:00
abstract::Structures of somatically acquired murine leukemia virus (MuLV) genomes present in the DNA of a large panel of MuLV-induced C57BL and BALB/c B and non-T/non-B cell lymphomas were compared with those present in MuLV-induced T-cell lymphomas induced in the same low-"spontaneous"-lymphoma-incidence mice. Analyses were pe...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.57.3.1037-1047.1986
更新日期:1986-03-01 00:00:00
abstract::A major determinant of neurovirulence for the GDVII strain of Theiler's virus, a murine picornavirus, was mapped to the P1 capsid protein region. Chimeric viruses were constructed by using sequences from the 5' noncoding and P1 regions of the virulent GDVII strain to replace equivalent regions of the less virulent BeA...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.7.3901-3907.1993
更新日期:1993-07-01 00:00:00
abstract::Infectious bursal disease virus (IBDV), a double-stranded RNA (dsRNA) virus belonging to the Birnaviridae family, is an economically important avian pathogen. The IBDV capsid is based on a single-shelled T=13 lattice, and the only structural subunits are VP2 trimers. During capsid assembly, VP2 is synthesized as a pro...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00077-07
更新日期:2007-07-01 00:00:00
abstract::Respiratory syncytial virus (RSV) infection of children previously immunized with a nonlive, formalin-inactivated (FI)-RSV vaccine has been associated with serious enhanced respiratory disease (ERD). Consequently, detailed studies of potential ERD are a critical step in the development of nonlive RSV vaccines targetin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02180-16
更新日期:2017-03-29 00:00:00
abstract::Although several virologic and immunologic factors associated with an increased risk of perinatal human immunodeficiency virus type 1 (HIV-1) transmission have been described, the mechanism of mother-to-child transmission is still unclear. More specifically, the question of whether selective pressures influence the tr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.5.3050-3057.2003
更新日期:2003-03-01 00:00:00
abstract::Antigens derived from host cells are detectable in the envelope of human immunodeficiency virus type 1 (HIV-1) and result in a distinctive viral phenotype reflecting that of the host cell. An immunomagnetic capture assay targeting discriminatory host proteins was developed to differentiate between HIV-1 derived from m...
journal_title:Journal of virology
pub_type: 杂志文章
doi:
更新日期:2000-01-01 00:00:00
abstract::The human immunodeficiency virus type 1 (HIV-1) entry inhibitor eCD4-Ig is a fusion of CD4-Ig and a coreceptor-mimetic peptide. eCD4-Ig is markedly more potent than CD4-Ig, with neutralization efficiencies approaching those of HIV-1 broadly neutralizing antibodies (bNAbs). However, unlike bNAbs, eCD4-Ig neutralized al...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02011-17
更新日期:2018-05-29 00:00:00
abstract:UNLABELLED:Herpes simplex virus 1 (HSV-1) glycoprotein B (gB)-specific CD8(+) T cells protect mice from herpes infection and disease. However, whether and which HSV-1 gB-specific CD8(+) T cells play a key role in the "natural" protection seen in HSV-1-seropositive healthy asymptomatic (ASYMP) individuals (who have neve...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03419-14
更新日期:2015-04-01 00:00:00
abstract::The spike (S) protein of the severe acute respiratory syndrome coronavirus (SARS-CoV) can be proteolytically activated by cathepsins B and L upon viral uptake into target cell endosomes. In contrast, it is largely unknown whether host cell proteases located in the secretory pathway of infected cells and/or on the surf...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02232-10
更新日期:2011-05-01 00:00:00
abstract::Kaposi's sarcoma-associated herpesvirus (KSHV) encodes a cluster of 12 microRNAs (miRNAs) that are processed from a transcript that is embedded within the major latency control region. We have generated a deletion mutation that eliminates 10 of the 12 viral miRNAs from the KSHV bacmid by using recombineering methods. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01997-09
更新日期:2010-03-01 00:00:00
abstract::In vitro studies have indicated that retroviral nucleocapsid (NC) protein facilitates both DNA synthesis by reverse transcriptase (RT) and annealing of the nascent DNA with acceptor template. Increasing the rate of DNA synthesis is expected to reduce the frequency of RT template switching, whereas annealing the nascen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.15.7473-7484.2002
更新日期:2002-08-01 00:00:00
abstract:UNLABELLED:During hepatitis C virus (HCV) infection, broadly neutralizing antibody (bNAb) responses targeting E1E2 envelope glycoproteins are generated in many individuals. It is unclear if these antibodies play a protective or a pathogenic role during chronic infection. In this study, we investigated whether bNAb resp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02669-15
更新日期:2016-04-14 00:00:00
abstract::In cell-free systems, 2-5A [ppp(A2'p)nA, n = 2 to greater than or equal to 4] activates a latent endoribonuclease, the 2-5A-dependent RNase, which cleaves rRNA in intact ribosomes into discrete and characteristic products (D. H. Wreschner et al., Nucleic Acids Res. 9:1571-1581, 1981). Here we present Northern blots wh...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.46.3.1051-1055.1983
更新日期:1983-06-01 00:00:00
abstract::The cellular requirements for activation of herpesvirus fusion and entry remain poorly understood. Low pH triggers change in the antigenic reactivity of the prefusion form of the herpes simplex virus (HSV) fusion protein gB in virions, both in vitro and during viral entry via endocytosis (S. Dollery et al., J. Virol. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05291-11
更新日期:2011-10-01 00:00:00
abstract::The interrelationship of viral ribonucleic acid (RNA) and protein synthesis in cells infected by Sindbis virus was investigated. When cultures were treated with puromycin early in the course of infection, the synthesis of interjacent RNA (26S) was preferentially inhibited. A similar result was obtained by shifting cel...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.4.2.117-122.1969
更新日期:1969-08-01 00:00:00
abstract::Herpesviruses are members of a diverse family of viruses that colonize all vertebrates from fish to mammals. Although more than one hundred herpesviruses exist, all are nearly identical architecturally, with a genome consisting of a linear double-stranded DNA molecule (100 to 225 kbp) protected by an icosahedral capsi...
journal_title:Journal of virology
pub_type: 杂志文章,评审
doi:10.1128/JVI.01169-10
更新日期:2010-12-01 00:00:00
abstract::In herpes simplex virus type 2, the mRNAs of ICP4 and ICP22/47 are divergently transcribed and their transcription initiation sites are separated by 750 base pairs (L. J. Whitton and J. B. Clements, Nucleic Acids Res. 12:2061-2078, 1984). This 750-base-pair region contains many recognized cis-acting elements, includin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.5.2036-2047.1989
更新日期:1989-05-01 00:00:00
abstract::Little is known about the transmission or tropism of the newly discovered human retrovirus, human T-cell lymphotropic virus type 3 (HTLV-3). Here, we examine the entry requirements of HTLV-3 using independently expressed Env proteins. We observed that HTLV-3 surface glycoprotein (SU) binds efficiently to both activate...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02285-08
更新日期:2009-05-01 00:00:00
abstract::Neonatal infection of most mouse strains with lymphocytic choriomeningitis virus (LCMV) leads to a life-long persistent infection characterized by high virus loads in the central nervous system (CNS) in the absence of inflammation and tissue destruction. These mice, however, exhibit impaired learning and memory. The o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00795-06
更新日期:2006-09-01 00:00:00