Abstract:
:The expression strategy of parvoviruses of the Densovirus genus has as yet not been reported. Clones were obtained from the densonucleosis virus of Galleria mellonella (GmDNV) that yielded infectious virus upon transfection into LD652 cells. Its genome was found to be the longest (6,039 nucleotides [nt]), with the largest inverted terminal repeats (ITRs) (550 nt) among all parvoviruses. The distal 136 nt could be folded into hairpins with flop or flip sequence orientations. In contrast to vertebrate parvoviruses, the gene cassettes for the nonstructural (NS) and structural (VP) proteins were found on the 5' halves of the opposite strands. The transcripts for both cassettes started 23 nt downstream of the ITRs. The TATA boxes, as well as all upstream promoter elements, were localized in the ITRs and, therefore, identical for the NS and VP transcripts. These transcripts overlapped for 60 nt at the 3' ends (antisense RNAs) at 50 m.u. The NS cassette consisted of three genes of which NS2 was contained completely within NS1 but from a different reading frame. Most of the NS transcripts were spliced to remove the upstream NS3, allowing leaky scanning translation of NS1 and NS2, similar to the genes of RNA-6 of influenza B virus. NS3 could be translated from the unspliced transcript. The VP transcript was not spliced and generated four VPs by a leaky scanning mechanism. The 5'-untranslated region of the VP transcript was only 5 nt long. Despite the transcription and translation strategies being radically different from those of vertebrate parvoviruses, the capsid was found to have phospholipase A(2) activity, a feature thus far unique for parvoviruses.
journal_name
J Viroljournal_title
Journal of virologyauthors
Tijssen P,Li Y,El-Far M,Szelei J,Letarte M,Zádori Zdoi
10.1128/jvi.77.19.10357-10365.2003subject
Has Abstractpub_date
2003-10-01 00:00:00pages
10357-65issue
19eissn
0022-538Xissn
1098-5514journal_volume
77pub_type
杂志文章abstract::Information was sought on the temporal distribution of transmissible mink encephalopathy virus in royal pastel mink inoculated subcutaneously with 10(3.0) 50% intracerebral lethal doses of the Idaho strain. As determined by intracerebral assay in mink, extremely little replication of the virus occurred during the prec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.10.3235-3240.1987
更新日期:1987-10-01 00:00:00
abstract::Minute virus of canines (MVC) is a member of the genus Bocavirus in the family Parvoviridae. We have molecularly cloned and sequenced the 5'- and 3'-terminal palindromes of MVC. The MVC genome, 5,404 nucleotides (nt) in length, shared an identity of 52.6% and 52.1% with that of human bocavirus and bovine parvovirus, r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02569-08
更新日期:2009-04-01 00:00:00
abstract::Vaccine-elicited immunoglobulin G (IgG) has been shown to be important for protection against simian-human immunodeficiency virus (SHIV) infection in rhesus monkeys. However, it remains unclear whether vaccine-elicited IgA responses are beneficial or detrimental for protection. In this study, we evaluated the kinetics...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00537-18
更新日期:2018-07-17 00:00:00
abstract::H9N2 avian influenza virus (AIV) has an extended host range, but the molecular basis underlying H9N2 AIV transmission to mammals remains unclear. We isolated more than 900 H9N2 AIVs in our 3-year surveillance in live bird markets in China from 2009 to 2012. Thirty-seven representative isolates were selected for furthe...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01141-16
更新日期:2016-10-14 00:00:00
abstract::A retrovirus vector has been developed that selects for instances in which the provirus integrates in close proximity to cellular promoters. Coding sequences for a selectable marker (histidinol dehydrogenase) were inserted into the 3' long terminal repeat (LTR) of an enhancerless Molony murine leukemia virus. Although...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.8.3227-3233.1989
更新日期:1989-08-01 00:00:00
abstract::Kaposi's sarcoma-associated herpesvirus (KSHV) encodes a cluster of 12 microRNAs (miRNAs) that are processed from a transcript that is embedded within the major latency control region. We have generated a deletion mutation that eliminates 10 of the 12 viral miRNAs from the KSHV bacmid by using recombineering methods. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01997-09
更新日期:2010-03-01 00:00:00
abstract::The buoyant density characteristics of infectious particles of herpes simplex virus types 1 and 2 were studied by centrifugation in sucrose and cesium chloride density gradients with a high resolution and satisfactory infectivity recovery. It was shown that two populations of infectious virions differing in buoyant de...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.38.2.737-748.1981
更新日期:1981-05-01 00:00:00
abstract::The Duplan strain of murine leukemia virus induces murine AIDS in C57BL/6 mice. When spleen cells from C57BL/6 mice infected with the virus were transplanted into nude mice, subcutaneous solid tumors at the transplanted sites were formed and splenomegaly and lymphadenopathy were induced. These transplantable cells wer...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.9.5691-5695.1992
更新日期:1992-09-01 00:00:00
abstract::The small RNA segment (S segment) of Uukuniemi (UUK) virus encodes two proteins, the nucleocapsid protein (N) and a nonstructural protein (NSs), by an ambisense strategy. The function of NSs has not been elucidated for any of the bunyaviruses expressing this protein. We have now expressed the N and NSs proteins in Sf9...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.7.4233-4241.1992
更新日期:1992-07-01 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) sequences were generated from blood and from brain tissue obtained by stereotactic biopsy from six patients undergoing a diagnostic neurosurgical procedure. Proviral DNA was directly amplified by nested PCR, and 8 to 36 clones from each sample were sequenced. Phylogenetic an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.11.7467-7481.1994
更新日期:1994-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.35.1.114-128.1980
更新日期:1980-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00209-06
更新日期:2006-07-01 00:00:00
abstract::Varicella-zoster virus (VZV) causes varicella and establishes latency in sensory nerve ganglia, but the characteristics of VZV latency are not well defined. Immunohistochemical detection of the VZV immediate-early 63 (IE63) protein in ganglion neurons has been described, but there are significant discrepancies in esti...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02416-09
更新日期:2010-04-01 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) infection of CD4-positive lymphocytes is accompanied by acute cytopathic effects, i.e., syncytium formation and single-cell lysis. Syncytium formation involves cell-cell fusion mediated by viral envelope glycoproteins on the surface of infected cells and by CD4 glycoproteins...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.3.1340-1354.1996
更新日期:1996-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.20.11097-11107.2004
更新日期:2004-10-01 00:00:00
abstract::Hepatitis C virus (HCV) is an enveloped positive-stranded RNA hepatotropic virus. HCV pseudoparticles infect liver-derived cells, supporting a model in which liver-specific molecules define HCV internalization. Three host cell molecules have been reported to be important entry factors or receptors for HCV internalizat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02286-07
更新日期:2008-05-01 00:00:00
abstract::Subgenomic RNAs of both polarities corresponding to rice hoja blanca virus (RHBV) ambisense RNA4 were detected in RHBV-infected rice tissues. Total RNA extracted from RHBV-infected and noninfected rice tissues and RNA4 purified from RHBV ribonucleoprotein particles were used as templates for primer extension studies. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.3.1951-1954.1995
更新日期:1995-03-01 00:00:00
abstract::Type 3 reovirus inhibits L cell DNA synthesis, whereas type 1 reovirus exerts little or no effect on L cell DNA synthesis. By using recombinant viruses containing both type 1 and type 3 double-standard RNA segments, we determined that one double-stranded RNA segment, the reovirus type 3 S1 double-stranded RNA segment ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.38.1.389-392.1981
更新日期:1981-04-01 00:00:00
abstract::Anti-VPg immunoprecipitable RNA labeled in vitro during a poliovirus RNA polymerase reaction was formed by the elongation of VPg-containing template fragments rather than by initiation with VPg. The reaction was dependent on a host factor (terminal uridylyl transferase). The incorporation of labeled UTP could be detec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.58.1.212-215.1986
更新日期:1986-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.17.3.906-915.1976
更新日期:1976-03-01 00:00:00
abstract::The majority of HIV-1 infections around the world result from non-B clade HIV-1 strains. The CRF01_AE (AE) strain is seen principally in Southeast Asia. AE protease differs by approximately 10% in amino acid sequence from clade B protease and carries several naturally occurring polymorphisms that are associated with d...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00505-10
更新日期:2010-10-01 00:00:00
abstract::Three serial passage series of simian virus 40 (SV40) in CV-1 cells were initiated by infection directly from the same wild-type plaque isolate, three series were initiated by infection with another plaque isolate, and two series were initiated with each of two other plaque isolates. Aberrant SV40 genomes were not det...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.42.2.730-733.1982
更新日期:1982-05-01 00:00:00
abstract::In a previous study (S. Zheng, G. N. Woode, D. R. Melendy, and R. F. Ramig, J. Clin. Microbiol. 27:1939-1945, 1989), it was predicted that the VP7 serotype 6 bovine rotavirus strains NCDV and B641 do not share antigenically similar VP4s. In this study, gene 4 and the VP7 gene of B641 were sequenced, and the amino acid...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.10.5535-5538.1991
更新日期:1991-10-01 00:00:00
abstract::Expression of the papillomavirus E4 protein correlates with the onset of viral DNA amplification. Using a mutant cottontail rabbit papillomavirus (CRPV) genome incapable of expressing the viral E4 protein, we have shown that E4 is required for the productive stage of the CRPV life cycle in New Zealand White and cotton...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.4.2142-2151.2004
更新日期:2004-02-01 00:00:00
abstract::During the 2009 H1N1 influenza virus pandemic (pdmH1N1) outbreak, it was found that most individuals lacked antibodies against the new pdmH1N1 virus, and only the elderly showed anti-hemagglutinin (anti-HA) antibodies that were cross-reactive with the new strains. Different studies have demonstrated that prior contact...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05540-11
更新日期:2012-02-01 00:00:00
abstract::Herpes simplex virus type 1 (HSV-1) DNA and RNA have been detected in peripheral nervous system (PNS) and central nervous system (CNS) tissues of latently infected mice. However, explant methods are successful in reactivating HSV-1 only from latently infected PNS tissues. In this report, latent herpesvirus infections ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.3.749-756.1988
更新日期:1988-03-01 00:00:00
abstract::Protective immunity to rotavirus (RV) is primarily mediated by antibodies produced by RV-specific memory B cells (RV-mBc). Of note, most of these cells express IgM, but the function of this subset is poorly understood. Here, using limiting dilution assays of highly sort-purified human IgM(+) mBc, we found that 62% and...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01466-12
更新日期:2012-10-01 00:00:00
abstract:UNLABELLED:Aminoquinolines and piperazines, linked or not, have been used successfully to treat malaria, and some molecules of this family also exhibit antiviral properties. Here we tested several derivatives of 4-aminoquinolines and piperazines for their activity against hepatitis C virus (HCV). We screened 11 molecul...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00404-16
更新日期:2016-09-12 00:00:00
abstract::The replication of the simian herpesvirus SA8 in Vero cells was examined. The time course of replication of the simian herpesvirus SA8 was found to be similar to that of the herpes simplex viruses. Infectious progeny virions were first detectable by 6 h postinfection and were readily released into the extracellular fl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.50.2.316-324.1984
更新日期:1984-05-01 00:00:00
abstract::We previously showed that the cellular proteins ZEB1 and ZEB2/SIP1 both play key roles in regulating the latent-lytic switch of Epstein-Barr Virus (EBV) by repressing BZLF1 gene expression. We investigated here the effects of cellular microRNA (miRNA) 200 (miR200) family members on the EBV infection status of cells. W...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00923-10
更新日期:2010-10-01 00:00:00