Abstract:
:We previously showed that the cellular proteins ZEB1 and ZEB2/SIP1 both play key roles in regulating the latent-lytic switch of Epstein-Barr Virus (EBV) by repressing BZLF1 gene expression. We investigated here the effects of cellular microRNA (miRNA) 200 (miR200) family members on the EBV infection status of cells. We show that miR200b and miR429, but not miR200a, can induce EBV-positive cells into lytic replication by downregulating expression of ZEB1 and ZEB2, leading to production of infectious virus. The levels of miR200 family members in EBV-infected cells strongly negatively correlated with the levels of the ZEBs (e.g., -0.89 [P < 0.001] for miR429 versus ZEB1) and positively correlated with the degree of EBV lytic gene expression (e.g., 0.73 [P < 0.01] for miR429 versus BZLF1). The addition of either miR200b or miR429 to EBV-positive cells led to EBV lytic reactivation in a ZEB-dependent manner; inhibition of these miRNAs led to decreased EBV lytic gene expression. The degree of latent infection by an EBV mutant defective in the primary ZEB-binding site of the EBV BZLF1 promoter was not affected by the addition of these miRNAs. Furthermore, EBV infection of primary blood B cells led to downregulation of these miRNAs and upregulation of ZEB levels. Thus, we conclude that miRNAs 200b and 429 are key regulators via their effects on expression of ZEB1 and ZEB2 of the switch between latent and lytic infection by EBV and, therefore, potential targets for development of new lytic induction therapeutics with which to treat patients with EBV-associated malignancies.
journal_name
J Viroljournal_title
Journal of virologyauthors
Ellis-Connell AL,Iempridee T,Xu I,Mertz JEdoi
10.1128/JVI.00923-10subject
Has Abstractpub_date
2010-10-01 00:00:00pages
10329-43issue
19eissn
0022-538Xissn
1098-5514pii
JVI.00923-10journal_volume
84pub_type
杂志文章abstract::A receptor on YAC-1 cells, a mouse T-lymphoma cell line, bound all six serotypes of the group B coxsackieviruses (CVB). In addition, the cells produced infectious virus. Each of the CVB competed for the same receptor on YAC-1 cells. CVB3 bound relatively slowly to YAC-1 cells (k = 4 x 10(-11) min-1 cell-1), and there ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.7.3105-3108.1989
更新日期:1989-07-01 00:00:00
abstract::Growth kinetics in lymphocytic H9 and M8166 cells of two mutants of human immunodeficiency virus type 1 (HIV-1) with deleted gp41 cytoplasmic tails were examined. While the mutant viruses designated CTdel-44 and CTdel-144 were able to grow in M8166 cells, they were unable to grow in H9 cells. Transfection and single-r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.10.4891-4893.2000
更新日期:2000-05-01 00:00:00
abstract::Major neutralization antigenic sites have been previously mapped by us on VP1, the largest capsid protein of poliovirus type 1. Here we report the first identification of the primary sequence of a neutralization antigenic site on capsid protein VP2. Inspection of the amino acid sequence of VP2 led to the selection and...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.52.2.719-721.1984
更新日期:1984-11-01 00:00:00
abstract::Vaccinia virus has a wide host range and infects mammalian cells of many different species. This suggests that the cell surface receptors for vaccinia virus are ubiquitously expressed and highly conserved. Alternatively, different receptors are used for vaccinia virus infection of different cell types. Here we report ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.2.1577-1585.1998
更新日期:1998-02-01 00:00:00
abstract::Human cytomegalovirus (HCMV) infection has been shown to activate the mTORC1 signaling pathway. However, the phosphorylation of mTORC1 targets is differentially sensitive to the mTORC1 inhibitor rapamycin, and the drug inhibits HCMV replication to a modest extent. Using Torin1, a newly developed inhibitor that targets...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02733-09
更新日期:2010-05-01 00:00:00
abstract::The genome of bacteriophage phi 6 contains three segments of double-stranded RNA. Procapsid structures whose formation was directed by cDNA copies of the large genomic segment are capable of packaging the three viral message sense RNAs in the presence of ATP. Addition of UTP, CTP, and GTP results in the synthesis of m...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.5.2611-2616.1992
更新日期:1992-05-01 00:00:00
abstract::Mitochondria are important for the viral life cycle, mainly by providing the energy required for viral replication and assembly. A highly complex interaction with mitochondria is exerted by rubella virus (RV), which includes an increase in the mitochondrial membrane potential as a general marker for mitochondrial acti...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00533-13
更新日期:2013-08-01 00:00:00
abstract::Viral recombination has been postulated to play two roles in the development of human immunodeficiency virus (HIV) resistance to antiretroviral drugs. First, recombination has the capacity to associate resistance mutations expressed by distinct viruses, thereby contributing to the development of viruses with improved ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00083-07
更新日期:2007-07-01 00:00:00
abstract::Brome mosaic virus (BMV), a member of the alphavirus-like super-family of positive-strand RNA viruses, encodes two proteins required for viral RNA replication: 1a and 2a. 1a contains m7G methyltransferase- and helicase-like domains, while 2a contains a polymerase (pol)-like core flanked by N- and C-terminal extensions...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.7.4729-4736.1996
更新日期:1996-07-01 00:00:00
abstract::Replication of poliovirus (PV) is restricted to a few sites, including the brain and spinal cord. However, this neurotropism is not conserved in cultured cells. Monkey kidney cells become susceptible to PV infection after cultivation in vitro, and cell lines of monolayer cultures from almost any tissue of primates are...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.9.4313-4325.2006
更新日期:2006-05-01 00:00:00
abstract:UNLABELLED:The lipid kinase phosphatidylinositol 4-kinase III alpha (PI4KIIIα) is an endoplasmic reticulum (ER)-resident enzyme that synthesizes phosphatidylinositol 4-phosphate (PI4P). PI4KIIIα is an essential host factor for hepatitis C virus (HCV) replication. Interaction with HCV nonstructural protein 5A (NS5A) lea...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01063-14
更新日期:2014-09-01 00:00:00
abstract::Prestimulation of the TLR4 pathway with lipopolysaccharide (LPS) protects mice from lethal infection with H5N1 influenza virus. Here, we reveal that the TLR4-TRIF pathway is required for this protective effect by using mice whose TLR4-related molecules were knocked out. Microarray analysis of primary mouse lung cultur...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06168-11
更新日期:2012-01-01 00:00:00
abstract::Fluorosulfonylbenzoyl 5'-adenosine (FSBA) bound to one site in simian virus 40 large T antigen (T) and covalently modified greater than 95% of the molecules in a complete reaction. This analog for ATP specifically cross-links to the Mg-phosphate pocket in ATP-binding sites. Cyanogen bromide cleavage and tryptic digest...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.10.4939-4947.1990
更新日期:1990-10-01 00:00:00
abstract::Broad, multispecific CD4(+) and CD8(+) T-cell responses to the hepatitis C virus (HCV), as well as virus-cross-neutralizing antibodies, are associated with recovery from acute infection and may also be associated in chronic HCV patients with a favorable response to antiviral treatment. In order to recapitulate all of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02743-07
更新日期:2008-08-01 00:00:00
abstract::The genomic RNA 3' noncoding region is believed to be a major cis-acting molecular genetic determinant for regulating picornavirus negative-strand RNA synthesis by promoting replication complex recognition. We report the replication of two picornavirus RNAs harboring complete deletions of the genomic RNA 3' noncoding ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.11.8868-8874.1997
更新日期:1997-11-01 00:00:00
abstract::An immunodominant envelope glycoprotein is encoded by the human herpesvirus 8 (HHV-8) (also termed Kaposi's sarcoma-associated herpesvirus) K8.1 gene. The functional role of glycoprotein K8.1 is unknown, and recognizable sequence homology to K8.1 is not detectable in the genomes of most other closely related gammaherp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.23.11583-11593.2001
更新日期:2001-12-01 00:00:00
abstract::In intact Madin-Darby canine kidney (MDCK) cell monolayers, vesicular stomatitis virus (VSV) matures only at basolateral membranes beneath tight junctions, whereas influenza virus buds from apical cell surfaces. Early in the growth cycle, the viral glycoproteins are restricted to the membrane domain from which each vi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.40.3.848-860.1981
更新日期:1981-12-01 00:00:00
abstract::The S protein of bovine coronavirus (BCV) has been isolated from the viral membrane and purified by gradient centrifugation. Purified S protein was identified as a viral hemagglutinin. Inactivation of the cellular receptors by sialate 9-O-acetylesterase and generation of receptors by sialylation of erythrocytes with N...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.11.6232-6237.1991
更新日期:1991-11-01 00:00:00
abstract::Inhibition of hepatitis B virus (HBV) replication and viral clearance from an infected host requires both the innate and adaptive immune responses. Expression of interferon (IFN)-inducible proteasome catalytic and regulatory subunits correlates with the IFN-alpha/beta- and IFN-gamma-mediated noncytopathic inhibition o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01779-06
更新日期:2007-01-01 00:00:00
abstract::Marburg virus (MARV) has been associated with sporadic episodes of hemorrhagic fever, including a recent highly publicized outbreak in Angola that produced severe disease and significant mortality in infected patients. MARV is also considered to have potential as a biological weapon. Recently, we reported the developm...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00959-06
更新日期:2006-10-01 00:00:00
abstract:UNLABELLED:Human immunodeficiency virus type 1 (HIV-1) replication in dendritic cells (DCs) is restricted by SAMHD1. This factor is counteracted by the viral protein Vpx; Vpx is found in HIV-2 and simian immunodeficiency virus (SIV) from sooty mangabeys (SIVsm) or from macaques (SIVmac) but is absent from HIV-1. We pre...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03057-13
更新日期:2014-05-01 00:00:00
abstract::Antibody to the capsid (PORF2) protein of hepatitis E virus (HEV) is sufficient to confer immunity, but knowledge of B-cell epitopes in the intact capsid is limited. A panel of murine monoclonal antibodies (MAbs) was generated following immunization with recombinant ORF2.1 protein, representing the C-terminal 267 amin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.17.8011-8017.2000
更新日期:2000-09-01 00:00:00
abstract::Growing CV1 cells were infected with simian virus 40 (SV40), and the levels of DNA polymerases-alpha, -beta, and -gamma were analyzed in the cytoplasm, nuclear Triton wash, and nucleus. In the cytoplasmic fraction, the amount of alpha-, beta-, or gamma-polymerase remained unaltered after SV40 infection. The activity o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.23.1.117-125.1977
更新日期:1977-07-01 00:00:00
abstract::The number, chromosomal distribution, and insertional polymorphisms of endogenous feline leukemia viruses (enFeLVs) were determined in four domestic cats (Burmese, Egyptian Mau, Persian, and nonbreed) using fluorescent in situ hybridization and radiation hybrid mapping. Twenty-nine distinct enFeLV loci were detected a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.7.3979-3986.2005
更新日期:2005-04-01 00:00:00
abstract::The active template for RNA synthesis for vesicular stomatitis virus (VSV) and other negative-strand viruses is the RNA genome in association with the nucleocapsid (N) protein. The N protein molecules sequester the genomic RNA and are linked together by a network of noncovalent interactions. We previously demonstrated...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01238-12
更新日期:2012-10-01 00:00:00
abstract::The development of immunization strategies to protect against ocular infection with herpes simplex virus 1 (HSV-1) must address the issue of the effects of the strategy on the establishment of latency in the trigeminal ganglia (TG). It is the reactivation of this latent virus that can cause recurrent disease and corne...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00913-14
更新日期:2014-07-01 00:00:00
abstract::Substitution of a methionine residue at position 79 in poliovirus protein 3A with valine or threonine caused defective viral RNA synthesis, manifested as delayed onset and reduced yield of viral RNA, in HeLa cells transfected with a luciferase-containing replicon. Viruses containing these same mutations produced small...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.23.12679-12691.2003
更新日期:2003-12-01 00:00:00
abstract::Direct evidence is presented for a host-specific role of the cucumovirus capsid protein in long-distance movement within infected plants. Cucumber (Cucumis sativus L.) is a systemic host for cucumber mosaic cucumovirus (CMV). Tomato aspermy cucumovirus, strain 1 (1-TAV), multiplied to the levels of CMV (i.e., replicat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.2.916-922.1995
更新日期:1995-02-01 00:00:00
abstract::Adenovirus type 2 (Ad2) and Ad5 enter epithelial cells via the coxsackievirus B Ad receptor (CAR) and alpha(v) integrin coreceptors. In the absence of CAR, they can be directed to the Fcgamma receptor 1 of hematopoietic cells by an adaptor comprising the extracellular CAR domain and the Fc portion of a human immunoglo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.4.2604-2613.2005
更新日期:2005-02-01 00:00:00
abstract::Human trophoblast cells were permissively infected by human cytomegalovirus. The kinetics of viral immediate-early, early, and late gene expression was clearly delayed compared to that in fibroblasts. Productive infection was unequivocally proven by the detection of virion particles, infectious virus in trophoblast cu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.9.7598-7602.1998
更新日期:1998-09-01 00:00:00