Abstract:
:Direct evidence is presented for a host-specific role of the cucumovirus capsid protein in long-distance movement within infected plants. Cucumber (Cucumis sativus L.) is a systemic host for cucumber mosaic cucumovirus (CMV). Tomato aspermy cucumovirus, strain 1 (1-TAV), multiplied to the levels of CMV (i.e., replicated, moved from cell to cell, and formed infectious particles) in the inoculated leaves of cucumbers but was completely unable to spread systemically. The defective long-distance systemic movement of 1-TAV was complemented by CMV in mixed infections. Coinfection of cucumbers with 1-TAV RNA with various combinations of transcripts from full-length cDNA clones of CMV genomic RNA 1, RNA2, and RNA3 showed that CMV RNA3 alone complemented 1-TAV long-distance movement. We obtained mutants containing mutations in the two open reading frames in CMV RNA3 encoding the 3a protein and the capsid protein (CP), both of which are necessary for cell-to-cell movement of CMV. Complementation experiments with mutant CMV RNA3 showed that only 3a protein mutants, i.e., those with an intact CP, complemented the long-distance movement of 1-TAV in cucumbers. Since CMV and TAV have common systemic host plants, the results presented here are strong evidence for an active, host-specific function of the CPs of these two cucumoviruses for long-distance spread in the phloem. The results also suggest that the plasmodesmata in the vascular system and/or at the boundary between the mesophyll and the vascular system, involved in long-distance movement through the phloem, and those in the mesophyll, involved in cell-to-cell movement, differ functionally.
journal_name
J Viroljournal_title
Journal of virologyauthors
Taliansky ME,García-Arenal Fdoi
10.1128/JVI.69.2.916-922.1995subject
Has Abstractpub_date
1995-02-01 00:00:00pages
916-22issue
2eissn
0022-538Xissn
1098-5514journal_volume
69pub_type
杂志文章abstract::Cell cycle progression during cytomegalovirus infection was investigated by fluorescence-activated cell sorter (FACS) analysis of the DNA content in growth-arrested as well as serum-stimulated human fibroblasts. Virus-infected cells maintained in either low (0.2%) or high (10%) serum failed to progress into S phase an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.2.1629-1634.1997
更新日期:1997-02-01 00:00:00
abstract::The prevalence and importance of microRNAs (miRNAs) in viral infection are increasingly relevant. Eleven miRNAs were previously identified in human cytomegalovirus (HCMV); however, miRNA content in murine CMV (MCMV), which serves as an important in vivo model for CMV infection, has not previously been examined. We hav...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01290-07
更新日期:2007-12-01 00:00:00
abstract::The interferon response elicited by Salmonella typhimurium mutants in mice is not dependent on the presence of a complete cell wall lipopolysaccharide. In fact, a mutant (G30/C21) which has lost all the polysaccharide side chains and sugars of the O antigen and contains only 2-keto-3-deoxyoctonate and lipid is indisti...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.1.6.1164-1167.1967
更新日期:1967-12-01 00:00:00
abstract::Harvard Medical School convened a meeting of biomedical and clinical experts on 5 March 2015 on the topic of "Rethinking the Response to Emerging Microbes: Vaccines and Therapeutics in the Ebola Era," with the goals of discussing the lessons from the recent Ebola outbreak and using those lessons as a case study to aid...
journal_title:Journal of virology
pub_type: 共识发展会议,杂志文章
doi:10.1128/JVI.01251-15
更新日期:2015-08-01 00:00:00
abstract::As viruses are reliant upon their host cell to serve as proper environments for their replication, many have evolved mechanisms to alter intracellular conditions to suit their own needs. For example, human cytomegalovirus induces quiescent cells to enter the cell cycle and then arrests them in late G(1), before they e...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.6.3451-3459.2003
更新日期:2003-03-01 00:00:00
abstract::The role of nitric oxide after poliovirus infection of the human HeLa (carcinoma) and U937 (promonocytic) cell lines has been analyzed. Both types of cells produced detectable levels of nitric oxide after poliovirus infection. However, this production was not sufficient to limit viral productivity. On the other hand, ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.3.2538-2540.1998
更新日期:1998-03-01 00:00:00
abstract::Feline herpesvirus produces characteristic morphological alterations in feline kidney cells. Nucleocapsid particles are formed in infected nuclei and are enveloped as they pass through the modified inner nuclear membrane. Aggregates of dense granular material and filamentous structures also regularly appear in infecte...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.5.5.617-623.1970
更新日期:1970-05-01 00:00:00
abstract::Latent Epstein-Barr virus (EBV) infection is associated with several lymphoproliferative disorders, including posttransplant lymphoma, Hodgkin's disease, and Burkitt's lymphoma, as well as nasopharyngeal carcinoma (NPC). Twenty-nine microRNAs (miRNAs) have been identified that are transcribed during latent infection f...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00785-08
更新日期:2008-09-01 00:00:00
abstract::Walleye dermal sarcoma virus (WDSV) is a complex retrovirus associated with dermal sarcomas in walleye fish. A WDSV accessory gene encodes a cyclin homolog or retroviral cyclin (rv-cyclin). WDSV rv-cyclin was found to be associated with transcription complexes and to affect transcription in a cell-type and promoter-de...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01425-06
更新日期:2006-12-01 00:00:00
abstract::We have investigated the species-specific replication of polyomavirus DNA in the cell-free system that was established previously (Y. Murakami, T. Eki, M. Yamada, C. Prives, and J. Hurwitz, Proc. Natl. Acad. Sci. USA 83:6347-6351, 1986). Extracts from various species of cells supported polyomavirus DNA replication in ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.9.4874-4881.1991
更新日期:1991-09-01 00:00:00
abstract::We compared the intracellular location of the product of the c-fps proto-oncogene, NCP98, with that of its viral homolog P140, the transforming protein of Fujinami sarcoma virus. Using the technique of biochemical subcellular fractionation, we determined that 60 to 90% of NCP98 and its associated kinase activity are i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.52.3.913-918.1984
更新日期:1984-12-01 00:00:00
abstract::The Cas-Br-E murine leukemia virus is a nondefective retrovirus that induces non-T-, non-B-cell lymphomas in susceptible NIH/Swiss mice. By using a DNA probe derived from Cas-Br-E provirus-flanking sequences, we identified a DNA region, originally called Sic-1, rearranged in 16 of 24 tumors analyzed (67%). All proviru...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.1.7-15.1991
更新日期:1991-01-01 00:00:00
abstract::Three drug-dependent mutants of human rhinovirus 16 (HRV16) were characterized by sequence analyses of spontaneous mutant isolates and were genetically reconstructed from a parental cDNA plasmid. These mutants formed plaques in the presence but not in the absence of the selecting antiviral drug, WIN 52035, which binds...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.2.1210-1218.1998
更新日期:1998-02-01 00:00:00
abstract::Infection and transformation of chondrocytes by Rous sarcoma viruses (RSVs) (Schmidt-Ruppin, Prague) stimulated the rate of cell growth. In contrast, several transformation-defective (td) mutants (tdPRA, tdNY105, tdNY106, tdNY107, and tdNY108) retaining various sizes of the src gene did not stimulate cell growth, indi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.35.2.531-541.1980
更新日期:1980-08-01 00:00:00
abstract::Originally identified as the gamma interferon-inducing factor, interleukin-18 (IL-18) was rediscovered as a proinflammatory cytokine related to the IL-1 family of cytokines that plays an important role in both innate and adaptive immune responses against viruses and intracellular pathogens. Despite its importance in i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.24.12448-12456.2002
更新日期:2002-12-01 00:00:00
abstract::The infection of cells which belong to the B-cell lineage is thought to be the primary event leading to the phenotypic and functional alterations seen in the murine AIDS (M. Huang, C. Simard, D. Kay, and P. Jolicoeur, J. Virol. 65:6562-6571, 1991). Using in situ hybridization, we studied the time course of the anatomi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.3.1903-1912.1994
更新日期:1994-03-01 00:00:00
abstract::More than 200 open reading frames (ORFs) from the human cytomegalovirus genome have been reported as potentially coding for proteins. We have used two pattern-based in silico approaches to analyze this set of putative viral genes. With the help of an objective annotation method that is based on the Bio-Dictionary, a c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.7.4326-4344.2003
更新日期:2003-04-01 00:00:00
abstract::Virus-infected cells secrete a broad range of interferons (IFN) which confer resistance to yet uninfected cells by triggering the synthesis of antiviral factors. The relative contributions of the various IFN subtypes to innate immunity against virus infections remain elusive. IFN-alpha, IFN-beta, and other type I IFN ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00272-10
更新日期:2010-06-01 00:00:00
abstract::The bovine herpesvirus 1 (BHV-1) UL49 gene encodes a viral tegument protein termed VP22. UL49 homologs are conserved among alphaherpesviruses. Interestingly, the BHV-1 VP22 deletion mutant virus is asymptomatic and avirulent in infected cattle but produces only a slight reduction in titer in vitro. Attenuation of the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.75.17.8251-8258.2001
更新日期:2001-09-01 00:00:00
abstract::Infectious leukemia virus production by two chronically infected NIH/MOL lines was strongly inhibited by interferon treatment of the cells. The corresponding degree of inhibition in JLSV-11 cells was much lower. Multiplication of encephalomyocarditis virus in all three cell lines was barely affected by interferon trea...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.45.3.1017-1027.1983
更新日期:1983-03-01 00:00:00
abstract::Taking advantage of the wide tropism of baculoviruses (BVs), we constructed a recombinant BV (BV(CAR)) pseudotyped with human coxsackie B-adenovirus receptor (CAR), the high-affinity attachment receptor for adenovirus type 5 (Ad5), and used the strategy of piggybacking Ad5-green fluorescent protein (Ad5GFP) vector on ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00012-09
更新日期:2009-06-01 00:00:00
abstract::We report here that sex hormones modulate susceptibility to a sexually transmitted viral agent, herpes simplex virus type 2 (HSV-2), in a mouse model. Ovariectomized mice were administered either saline (control), estradiol (E(2)), progesterone (P(4)), or a combination of both estradiol and progesterone (E+P) and infe...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.5.3107-3116.2005
更新日期:2005-03-01 00:00:00
abstract::The herpes simplex virus type 1 (HSV-1) genome contains three origins of DNA replication, one copy of oriL and two copies of oriS. Although oriL and oriS are structurally different, they have extensive nucleotide sequence similarity and can substitute for each other to initiate viral DNA replication. A fundamental que...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.5.3580-3587.1997
更新日期:1997-05-01 00:00:00
abstract::Phage XP-12, which has complete substitution of the cytosine residues in its DNA with 5-methylcytosine residues, was shown to inhibit incorporation of uracil into host DNA and RNA during the latent period. This apparent inhibition of host macromolecular synthesis was not accompanied by extensive degradation of the hos...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.23.3.517-523.1977
更新日期:1977-09-01 00:00:00
abstract::The hepatitis B virus (HBV) X protein (HBx) is a multifunctional regulator of cellular signal transduction and transcription pathways and has a critical role in HBV replication. Much of the cytoplasmic signal transduction activity associated with HBx expression and its stimulation of viral replication is attributable ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.9.4406-4414.2006
更新日期:2006-05-01 00:00:00
abstract::The DNA product of the endogenously instructed RNA-dependent DNA polymerase reaction of murine sarcoma virus continued to be synthesized for as long as 64 h in the presence of 0.008% Triton X-100. Higher detergent concentrations and actinomycin D inhibited DNA product synthesis. The DNA product from long-term polymera...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.12.5.1049-1064.1973
更新日期:1973-11-01 00:00:00
abstract::Divergent Toll-like receptor 7 (TLR7) and TLR9 signaling has been proposed to distinguish pathogenic from nonpathogenic simian immunodeficiency virus infection in primate models. We demonstrate here that increased expression of type I interferon in pathogenic rhesus macaques compared to nonpathogenic African green mon...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01496-09
更新日期:2010-02-01 00:00:00
abstract::The 70S RNA of Rous sarcoma virus contains 4S RNAs which serve as primers for the initiation of DNA synthesis in vitro by the RNA-directed DNA polymerase of the virus. We purified these primers in three different ways-by isolation of the covalent complex between primer and nascent DNA, by differential melting of the 7...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.5.1134-1142.1974
更新日期:1974-05-01 00:00:00
abstract::Transmission of arenaviruses from rodent hosts to humans is generally thought to occur through inhalation or ingestion of dust or droplets containing viral particles. Here we demonstrate that two identified arenavirus receptors, alpha-dystroglycan (alpha-DG) and transferrin receptor 1 (TfR1), are expressed in polarize...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00100-08
更新日期:2008-06-01 00:00:00
abstract::Adenovirus types 5 and 3 (Ad5 and Ad3), two human adenovirus serotypes of evolutionarily divergent subgroups, show very different levels of E1A gene expression early after infection of permissive cells. Since adenovirus E1A gene expression is known to be transcriptionally autoregulated, we have investigated the differ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.4.1833-1838.1989
更新日期:1989-04-01 00:00:00