Abstract:
:Viral recombination has been postulated to play two roles in the development of human immunodeficiency virus (HIV) resistance to antiretroviral drugs. First, recombination has the capacity to associate resistance mutations expressed by distinct viruses, thereby contributing to the development of viruses with improved drug resistance. In addition, recombination could preserve diversity in regions outside those subject to strong selective pressure. In this study, we sought direct evidence for the occurrence of these processes in vivo by evaluating clonal virus populations obtained from the same patient before and after a treatment change that, while unsuccessful in controlling viral replication, led to the emergence of viruses expressing a different profile of resistance mutations. Phylogenetic studies supported the conclusion that the genotype arising after the treatment change resulted from the emergence of recombinant viruses carrying previously existing resistance mutations in novel combinations, whereas alternative explanations, including convergent evolution, were not consistent with observed genotypic changes. Despite evidence for a strong loss of genetic diversity in genomic regions coding for the protease and reverse transcriptase, diversity in regions coding for Gag and envelope was considerably higher, and recombination between the emerging viruses expressing the new pattern of resistance mutations and viral quasispecies in the previously dominant population contributed to this preservation of diversity in the envelope gene. These findings emphasize that recombination can participate in the adaptation of HIV to changing selective pressure, both by generating novel combinations of resistance mutations and by maintaining diversity in genomic regions outside those implicated in a selective sweep.
journal_name
J Viroljournal_title
Journal of virologyauthors
Nora T,Charpentier C,Tenaillon O,Hoede C,Clavel F,Hance AJdoi
10.1128/JVI.00083-07subject
Has Abstractpub_date
2007-07-01 00:00:00pages
7620-8issue
14eissn
0022-538Xissn
1098-5514pii
JVI.00083-07journal_volume
81pub_type
杂志文章abstract::Current human immunodeficiency virus type 1 (HIV-1) envelope vaccine candidates elicit high antibody binding titers with neutralizing activity against T-cell line-adapted but not primary HIV-1 isolates. Serum antibodies from these human vaccine recipients were also found to be preferentially directed to linear epitope...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.6.4319-4330.1997
更新日期:1997-06-01 00:00:00
abstract::Q beta phage RNAs with inactivating insertion (8-base) or deletion (17-base) mutations within their replicase genes were prepared from modified Q beta cDNAs and transfected into Escherichia coli spheroplasts containing Q beta replicase provided in trans by a resident plasmid. Replicase-defective (Rep-) Q beta phage pr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.4.2435-2442.1992
更新日期:1992-04-01 00:00:00
abstract::Scavenger receptor class B type I (SR-BI) is an essential receptor for hepatitis C virus (HCV) and a cell surface high-density-lipoprotein (HDL) receptor. The mechanism of SR-BI-mediated HCV entry, however, is not clearly understood, and the specific protein determinants required for the recognition of the virus envel...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02199-08
更新日期:2010-01-01 00:00:00
abstract::We provide here new insights into rotavirus (RRV) pathogenicity by showing that RRV infection promotes structural and functional injuries localized at the tight junctions (TJ) in the cell-cell junctional complex of cultured polarized human intestinal Caco-2 cells forming monolayers. RRV infection resulted in a progres...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.10.4645-4651.2000
更新日期:2000-05-01 00:00:00
abstract::Phylogenetic analyses demonstrate significant diversity in worldwide circulating strains of human immunodeficiency virus type 1 (HIV-1). Detailed studies have revealed a complex pattern of intersubtype recombinations, as well as evidence of sub-subtypes circulating in various populations. In this study, we characteriz...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.22.12438-12445.2004
更新日期:2004-11-01 00:00:00
abstract::The initial virus strains from as many as 12% of individuals with primary human immunodeficiency virus (HIV) infection have a 50% inhibitory concentration =0.4-fold that of HIV type 1(NL4-3) (HIV-1(NL4-3)) to ritonavir (hypersusceptibility [HS]). There is also substantial variation in replicative capacity (RC) or an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.5.2242-2246.2004
更新日期:2004-03-01 00:00:00
abstract::Kaposi's sarcoma-associated herpesvirus (KSHV) utilizes clathrin-mediated endocytosis for its infectious entry into human foreskin fibroblast (HFF) cells (S. M. Akula, P. P. Naranatt, N.-S. Walia, F.-Z. Wang, B. Fegley, and B. Chandran, J. Virol. 77:7978-7990, 2003). Here, we characterized KSHV entry into primary huma...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02498-08
更新日期:2009-05-01 00:00:00
abstract::Herpes simplex virus type 1 (HSV-1) is a DNA virus that acquires an envelope by budding into the inner nuclear membrane of an infected cell. Recombinant HSV-1 lacking the U(L)34 gene cannot undergo this event. U(L)34 and U(L)31, another viral protein, colocalize in an infected cell and are necessary and sufficient to ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.13.7601-7610.2003
更新日期:2003-07-01 00:00:00
abstract::The cytoplasmic assembly of vaccinia virus is reversibly blocked by the antibiotic rifampin, leading to the accumulation of partially membrane-delineated rifampin bodies in infected cells. Rifampin-resistant vaccinia virus mutants have point mutations in the D13L gene, which is controlled by a late promoter and expres...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.2.1103-1114.1994
更新日期:1994-02-01 00:00:00
abstract::Coinfection with HIV-1 and Kaposi's sarcoma-associated herpesvirus (KSHV) often leads to AIDS-related malignancies, including Kaposi's sarcoma (KS) and primary effusion lymphoma (PEL). The interaction between HIV and KSHV plays a pivotal role in the progression of these malignancies. We have previously demonstrated th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00580-18
更新日期:2018-08-29 00:00:00
abstract::Serial serum specimens from 22 herpes simplex virus (HSV)-seronegative recipients of an HSV type 2 (HSV-2) glycoprotein subunit vaccine were analyzed by radioimmunoprecipitation and polyacrylamide gel electrophoresis for the development of antibodies to HSV-2 gB, gD, and g80, a complex of gC and gE. Volunteers receive...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.56.2.475-481.1985
更新日期:1985-11-01 00:00:00
abstract::Autophagy and the effects of its inhibition or induction were investigated during the entire infectious cycle of varicella-zoster virus (VZV), a human herpesvirus. As a baseline, we first enumerated the number of autophagosomes per cell after VZV infection compared with the number after induction of autophagy followin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02646-13
更新日期:2014-01-01 00:00:00
abstract::Phage 2 adsorbed to Pseudomonas aeruginosa strain BI in 5 mM Tris buffer, providing that cations like Na(+), Mg(2+), or Ca(2+) were present. Adsorption was observed over a broad pH range, reaching a maximum level around pH 7.5, which coincided with the pH required for maximal activity of the phage 2-associated slime p...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.1.22-27.1974
更新日期:1974-01-01 00:00:00
abstract::Human parainfluenza type 2 virus (hPIV-2)-infected HeLa (HeLa-CA) cells and hPIV-2 V-expressing HeLa (HeLa-V) cells show high resistance to alpha/beta interferons (IFN-alpha/beta) irrespective of whether vesicular stomatitis virus or Sindbis virus is used as a challenge virus. When Sindbis virus is used, these cells s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.19.9165-9176.2001
更新日期:2001-10-01 00:00:00
abstract::The src genes of different Rous sarcoma virus (RSV) strains have been reported to be highly conserved by some investigators using RNA-cDNA hybridization, whereas others using oligonucleotide, peptide, and serological analyses have judged src genes to be variable in 30 to 50% of the respective markers. Moreover, distin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.39.3.758-776.1981
更新日期:1981-09-01 00:00:00
abstract::We have developed a system in which vesicular stomatitis virus (VSV) minigenomes encoding viral structural proteins can be expressed from plasmids. These RNAs can be replicated, transcribed, and packaged into infectious particles when coexpressed with the other VSV proteins. The minigenomes contain either the glycopro...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.5.2946-2953.1995
更新日期:1995-05-01 00:00:00
abstract::In bacterial strains containing the deoxyribonuclease endonuclease I (endonuclease I(+) strains), 70 to 80% of the injected superinfecting T-even phage deoxyribonucleic acid (DNA) is rapidly degraded to oligonucleotides having an average chain length of 8, the same value as that obtained by endonuclease I digestion of...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.8.6.869-886.1971
更新日期:1971-12-01 00:00:00
abstract::Rhesus cytomegalovirus (RhCMV) infection of macaques exhibits strong similarities to human CMV (HCMV) persistence and pathogenesis. The immunogenicity of DNA vaccines encoding three RhCMV proteins (a truncated version of glycoprotein B lacking the transmembrane region and endodomain [gBDeltaTM], phosphoprotein 65-2 [p...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01708-06
更新日期:2007-02-01 00:00:00
abstract::The terminal 500-base-pair alpha sequence of the herpes simplex virus 1 genome contains signals for cleavage (Pac1 and Pac2) of unit-length DNA molecules from concatemers in unique stretches of sequences designated Ub and Uc, respectively, and a cis site for cleavage designated DR1. We report that nuclear extracts fro...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.3.1059-1068.1989
更新日期:1989-03-01 00:00:00
abstract::Two prion strains with identical incubation periods in mice exhibited distinct incubation periods and different neuropathological profiles upon serial transmission to transgenic mice expressing chimeric Syrian hamster/mouse (MH2M) prion protein (PrP) genes [Tg(MH2M) mice] and subsequent transmission to Syrian hamsters...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.12.9032-9044.1997
更新日期:1997-12-01 00:00:00
abstract::The Epstein-Barr virus nuclear antigen 2 (EBNA-2) acidic domain is essential for B-lymphocyte growth transformation and can activate transcription when brought to a promoter by a sequence-specific DNA-binding domain. We now show that the EBNA-2 acidic domain has slightly less activity than the proteotypic acidic trans...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.1.585-588.1995
更新日期:1995-01-01 00:00:00
abstract::Tryptic digests of the internal proteins p30, p15, p12, and p10 of mouse xenotropic, ecotropic, and amphotropic type C viruses were subjected to cation-exchange chromatography. Analysis of these maps revealed that the p30 proteins from representative isolates of all three viral subgroups were distinguishable. The p15 ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.29.1.102-113.1979
更新日期:1979-01-01 00:00:00
abstract::Chikungunya virus (CHIKV), a mosquito-borne alphavirus, has traditionally circulated in Africa and Asia, causing human febrile illness accompanied by severe, chronic joint pain. In Africa, epidemic emergence of CHIKV involves the transition from an enzootic, sylvatic cycle involving arboreal mosquito vectors and nonhu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01603-09
更新日期:2010-07-01 00:00:00
abstract::Zika virus (ZIKV) envelope (E) protein is the major target of neutralizing antibodies in infected hosts and thus represents a candidate of interest for vaccine design. However, a major concern in the development of vaccines against ZIKV and the related dengue virus is the induction of cross-reactive poorly neutralizin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01415-20
更新日期:2020-12-09 00:00:00
abstract::GS-8374 is a potent HIV protease inhibitor (PI) with a unique diethyl-phosphonate moiety. Due to a balanced contribution of enthalpic and entropic components to its interaction with the protease (PR) active site, the compound retains activity against HIV mutants with high-level multi-PI resistance. We report here the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01211-12
更新日期:2013-01-01 00:00:00
abstract::Although xenotropic murine leukemia viruses cannot productively infect cells of laboratory mice, cells from various wild-derived mice can support replication of these viruses. Although the virus-sensitive wild mice generally lack all or most of the xenotropic proviral genes characteristic of inbred strains, susceptibi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.55.3.690-695.1985
更新日期:1985-09-01 00:00:00
abstract::Splicing of human immunodeficiency virus type 1 (HIV-1) exon 6D is regulated by the presence of a complex splicing regulatory element (SRE) sequence that interacts with the splicing factors hnRNP H and SC35. In this work, we show that, in the context of the wild-type viral sequence, hnRNP H acts as a repressor of exon...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00721-08
更新日期:2008-08-01 00:00:00
abstract::The process of Epstein-Barr virus (EBV)-induced transformation of human B lymphocytes results in a cell line that is a mixture of latently and lytically infected cells, with the lytic cells composing roughly 5% to less than 0.0001% of the overall population. A set of nine normal lymphoblastoid cell lines that span a 1...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.1.437-444.1990
更新日期:1990-01-01 00:00:00
abstract::Apoptosis is a type of controlled cell death that is essential for development and tissue homeostasis. It also serves as a robust host response against infection by many viruses. The capacity of neurotropic viruses to induce apoptosis strongly correlates with virulence. However, the precise function of apoptosis in vi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01931-13
更新日期:2013-12-01 00:00:00
abstract::The structural instability of inactivated foot-and-mouth disease virus (FMDV) hinders the development of vaccine industry. Here we found that some transition metal ions like Cu2+ and Ni2+ could specifically bind to FMDV capsids at capacities about 7089 and 3448 metal ions per capsid, respectively. These values are abo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02431-20
更新日期:2021-01-13 00:00:00