Abstract:
:Vaccine-elicited immunoglobulin G (IgG) has been shown to be important for protection against simian-human immunodeficiency virus (SHIV) infection in rhesus monkeys. However, it remains unclear whether vaccine-elicited IgA responses are beneficial or detrimental for protection. In this study, we evaluated the kinetics, magnitude, breadth, and linear epitope specificities of vaccine-elicited IgG and IgA responses in serum and mucosal secretions following intramuscular immunization with adenovirus 26 (Ad26) prime, Env protein boost vaccination regimens. The systemic and mucosal antibody responses exhibited kinetics similar to those of the serum antibody responses but lower titers than the serum antibody responses. Moreover, the IgG and IgA responses were correlated, both in terms of the magnitude of the responses and in terms of the antibody specificities against linear human immunodeficiency virus type 1 (HIV-1) Env, Gag, and Pol epitopes. These data suggest that IgG and IgA responses are highly coordinated in both peripheral blood and mucosal compartments following Ad26/Env vaccination in rhesus monkeys.IMPORTANCE Vaccine-elicited IgG responses are important for protection against simian-human immunodeficiency virus (SHIV) infection in nonhuman primates. However, much less is known about the role and function of IgA, despite it being the predominant antibody in mucosal sites. There is debate as to whether HIV-1-specific IgA responses are beneficial or detrimental, since serum anti-Env IgA titers were shown to be inversely correlated with protection in the RV144 clinical trial. We thus assessed vaccine-elicited IgG and IgA antibody responses in peripheral blood and mucosal secretions following vaccination with the Ad26/Env vaccine.
journal_name
J Viroljournal_title
Journal of virologyauthors
Kang ZH,Bricault CA,Borducchi EN,Stephenson KE,Seaman MS,Pau M,Schuitemaker H,van Manen D,Wegmann F,Barouch DHdoi
10.1128/JVI.00537-18subject
Has Abstractpub_date
2018-07-17 00:00:00issue
15eissn
0022-538Xissn
1098-5514pii
JVI.00537-18journal_volume
92pub_type
杂志文章abstract::Hepatitis B virus (HBV) is unusual in that its surface proteins (small [S], medium, and large [L]) are not only incorporated into the virion envelope but they also bud into empty subviral particles in great excess over virions. The morphogenesis of these subviral envelope particles remains unclear, but the S protein i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02741-06
更新日期:2007-04-01 00:00:00
abstract::Rabies, caused by rabies virus (RABV), is a fatal encephalitis in humans and other mammals, which continues to present a public health threat in most parts of the world. Our previous study demonstrated that Toll-like receptor 7 (TLR7) is essential in the induction of anti-RABV antibodies via the facilitation of germin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00111-20
更新日期:2020-04-16 00:00:00
abstract::The development of antiviral drug resistance is an important problem in the treatment of human immunodeficiency virus type 1 (HIV-1) infection. Potent antiretroviral therapy is currently used for treatment, and typically consists of at least two reverse transcriptase (RT) inhibitors. We have previously reported that b...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.18.12045-12057.2005
更新日期:2005-09-01 00:00:00
abstract::Recombinant adeno-associated virus (rAAV) vectors have been shown to be useful for efficient gene delivery to a variety of dividing and nondividing cells. Mechanisms responsible for the long-term, persistent expression of the rAAV transgene are not well understood. In this study we investigated the kinetics of rAAV-me...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.8.3555-3565.2000
更新日期:2000-04-01 00:00:00
abstract::The membrane-proximal region of the human immunodeficiency virus type 1 (HIV-1) transmembrane protein (TM) is critical for envelope (Env)-mediated membrane fusion and contains the target for broadly reactive neutralizing antibody 2F5. It has been proposed that 2F5 neutralization might involve interaction of its long, ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.5.2539-2547.2006
更新日期:2006-03-01 00:00:00
abstract::We present an antiviral-immunomodulatory therapeutic strategy involving the chemokine receptor antagonist Met-RANTES, which yields significant survival in the setting of an otherwise fatal respiratory virus infection. In previous work, we demonstrated that infection with the natural rodent pathogen pneumonia virus of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.15.7984-7989.2004
更新日期:2004-08-01 00:00:00
abstract::The v-rel oncogene encoded by reticuloendotheliosis virus strain T is the acutely transforming member of the Rel/NF-kappaB family of transcription factors. In v-Rel-transformed cells, v-Rel exists as homodimers or heterodimers with the endogenous Rel/NF-kappaB proteins c-Rel, NF-kappaB1, NF-kappaB2, and RelA. To exami...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.10.4928-4939.2002
更新日期:2002-05-01 00:00:00
abstract::We engineered a disulfide-stabilized influenza virus hemagglutinin (HA) trimer, termed HA3-SS, by introducing cysteine residues into the HA stem to covalently bridge the three protomers. HA3-SS has increased thermostability compared to wild-type HA, and binding of head- and stem-targeted antibodies (Abs) is preserved;...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00808-15
更新日期:2015-07-01 00:00:00
abstract::We have devised an enrichment scheme for the isolation of export-competent derivatives of pseudorabies virus glycoprotein gIII signal peptide mutants. Enrichment is based upon a growth advantage imparted upon gIII-containing virions compared with virions lacking the glycoprotein. Each of identified derivatives suppres...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.5.2787-2794.1994
更新日期:1994-05-01 00:00:00
abstract::Mitochondria are important for the viral life cycle, mainly by providing the energy required for viral replication and assembly. A highly complex interaction with mitochondria is exerted by rubella virus (RV), which includes an increase in the mitochondrial membrane potential as a general marker for mitochondrial acti...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00533-13
更新日期:2013-08-01 00:00:00
abstract::The latent membrane protein 1 (LMP-1) of Epstein-Barr virus (EBV) functionally resembles a constitutively active, CD40-like receptor and contributes to the maintenance of proliferation of EBV-infected primary human B lymphocytes. LMP-1 is targeted to the plasma membrane, where it binds TRAF, TRADD, and JAK molecules t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.20.9755-9761.2000
更新日期:2000-10-01 00:00:00
abstract::The Parvovirus H-1 replicates autonomously in hamster embryo cells. A DNA synthetic event, called HA-DNA synthesis, upon which subsequent viral RNA and viral hemagglutinin synthesis is dependent, is initiated in late S phase of the infected cell (18). It was postulated that HA-DNA represents parental viral replicative...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.2.400-410.1974
更新日期:1974-02-01 00:00:00
abstract::The coronavirus nucleocapsid (N) protein plays a multifunctional role in the virus life cycle, from regulation of replication and transcription and genome packaging to modulation of host cell processes. These functions are likely to be facilitated by interactions with host cell proteins. The potential interactome of t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00321-13
更新日期:2013-09-01 00:00:00
abstract::Following intranasal administration, the severe acute respiratory syndrome (SARS) coronavirus replicated to high titers in the respiratory tracts of BALB/c mice. Peak replication was seen in the absence of disease on day 1 or 2, depending on the dose administered, and the virus was cleared within a week. Viral antigen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.7.3572-3577.2004
更新日期:2004-04-01 00:00:00
abstract::The specificity of binding of wheat germ ribosomes to mRNA was greatly altered by cleavage of the message. Fragmentation of reovirus mRNA allowed wheat germ ribosomes to bind and protect a variety of internal sequences which were not accessible to ribosomes in the intact message. In experiments using the polycistronic...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.35.3.748-756.1980
更新日期:1980-09-01 00:00:00
abstract::The morphogenesis of vesicular stomatitis virus was examined using freeze-fracture techniques, and the results obtained were compared with those from previously published experiments carried out with influenza viruses and togaviruses. The process of conversion of the host cell plasma membrane into the vesicular stomat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.21.2.601-609.1977
更新日期:1977-02-01 00:00:00
abstract::Vesicular stomatitis virus (VSV) is a prototype nonsegmented, negative-sense virus used to examine viral functions of a broad family of viruses, including human pathogens. Here we demonstrate that S(2) VSV, an isolate with a small plaque phenotype compared to other Indiana strain viruses, has a transcription defect re...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00358-12
更新日期:2012-08-01 00:00:00
abstract::The protein kinase inhibitor H7 blocks influenza virus replication, inhibits production of the matrix protein (M1), and leads to a retention of the viral ribonucleoproteins (vRNPs) in the nucleus at late times of infection (K. Martin and A. Helenius, Cell 67:117-130, 1991). We show here that production of assembled vR...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.4.1781-1786.2000
更新日期:2000-02-01 00:00:00
abstract::The recently developed subgenomic hepatitis C virus (HCV) replicons were limited by the fact that the sequence encoding the structural proteins was missing. Therefore, important information about a possible influence of these proteins on replication and pathogenesis and about the mechanism of virus formation could not...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.8.4008-4021.2002
更新日期:2002-04-01 00:00:00
abstract:UNLABELLED:While numerous viral microRNAs (miRNAs) expressed by DNA viruses, especially herpesvirus family members, have been reported, there have been very few reports of miRNAs derived from RNA viruses. Here we describe three miRNAs expressed by bovine foamy virus (BFV), a member of the spumavirus subfamily of retrov...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03587-13
更新日期:2014-05-01 00:00:00
abstract::Human La protein has been implicated in facilitating internal ribosome entry site (IRES)-mediated translation of hepatitis C virus (HCV). Earlier, we demonstrated that the RNA recognition motif (RRM) encompassing residues 112 to 184 of La protein [La (112-184)] interacts with the HCV IRES near the initiator AUG codon....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00924-08
更新日期:2008-12-01 00:00:00
abstract::The nucleotide composition of the ribonucleic acid of three strains of rabies virus was determined and found to be similar to that of vesicular stomatitis virus. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.8.6.922-924.1971
更新日期:1971-12-01 00:00:00
abstract::Human herpesvirus 8 (HHV-8) is likely to be involved in the pathogenesis of Kaposi's sarcoma (KS) and body cavity-based lymphomas (BCBLs). The HHV-8 genome is primarily in a latent state in BCBL-derived cell lines like BCBL-1, but lytic replication can be induced by phorbol esters (R. Renne, W. Zhang, B. Herndier, M. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.8.6725-6731.1998
更新日期:1998-08-01 00:00:00
abstract::Despite reports of viral genetic defects in persons who control human immunodeficiency virus type 1 (HIV-1) in the absence of antiviral therapy, the extent to which such defects contribute to the long-term containment of viremia is not known. Most previous studies examining for such defects have involved small numbers...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00535-08
更新日期:2008-09-01 00:00:00
abstract::To facilitate the determination of the genomic location of the vaccinia virus gene(s) encoding alpha-amanitin resistance (alpha r) (Villarreal et al., J. Virol. 51:359-366, 1984), a collection of alpha r, temperature-sensitive (ts) mutants were isolated. The premise of these experiments was that mutants might be found...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.57.1.65-70.1986
更新日期:1986-01-01 00:00:00
abstract:UNLABELLED:The first discovered and sequenced hepatitis C virus (HCV) genome and the first in vivo infectious HCV clones originated from the HCV prototype strains HCV-1 and H77, respectively, both widely used in research of this important human pathogen. In the present study, we developed efficient infectious cell cult...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02877-14
更新日期:2015-01-01 00:00:00
abstract::Swainsonine, an inhibitor of glycoprotein processing, inhibits the formation of the normal oligosaccharide chain of the G protein of vesicular stomatitis virus. Thus, when vesicular stomatitis virus was grown in baby hamster kidney cells in the presence of swainsonine (15 to 500 ng/ml) and labeled with [2-(3)H]mannose...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.46.1.60-69.1983
更新日期:1983-04-01 00:00:00
abstract::We have purified the seven virus-specific RNAs which were previously shown to be induced in Sac(-) cells upon infection with mouse hepatitis virus strain A59 (W. J. M. Spaan, P. J. M. Rottier, M. C. Horzinek, and B. A. M. van der Zeijst, Virology 108:424-434, 1981). The individual RNAs, prepared by agarose gel electro...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.38.1.20-26.1981
更新日期:1981-04-01 00:00:00
abstract::Investigation of the human antibody response to the 1957 pandemic H2N2 influenza A virus has been largely limited to serologic studies. We generated five influenza virus hemagglutinin (HA)-reactive human monoclonal antibodies (MAbs) by hybridoma technology from the peripheral blood of healthy donors who were born betw...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.07158-11
更新日期:2012-06-01 00:00:00
abstract::Hemagglutinin (HA) of influenza virus must be activated by proteolysis before the virus can become infectious. Previous studies indicated that HA cleavage is driven by membrane-bound or extracellular serine proteases in the respiratory tract. However, there is still uncertainty as to which proteases are critical for a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00421-17
更新日期:2017-07-27 00:00:00