Abstract:
:Cell fractionation and protein electrophoresis were used to study the intracellular sites of synthesis and intermediate structures in the assembly of the virion proteins of vesicular stomatitis virus. Each of the three major virion proteins assembled into virions through a separable pathway. The nucleocapsid (N) protein was first a soluble protein and later incorporated into free, cytoplasmic nucleocapsids. A small amount of N protein was bound to membranes at later times, presumably representing either nucleocapsids in the process of budding or completed virions attached to the cell surface. The matrix (M) protein also appeared to be synthesized as a soluble protein, but was then directly incorporated into membranous structures with the same density as whole virus. Very little M protein was ever found in membranes banding at the density of plasma membranes. The M protein entered extracellular virus very quickly, as though it moved directly from a soluble state into budding virus. In contrast, the glycoprotein (G) was always membrane bound; it appeared to be directly inserted into membranes during its synthesis. Glycosylation of the G protein was completed only in smooth membrane fractions, possibly in the Golgi apparatus. After a minimum time of 15 min following its synthesis, G protein was incorporated into the surface plasma membrane, from which it was slowly shed into virions. These multiple processing steps probably account for its delayed appearance in virus. From this work it appears that the three major structural proteins come into the surface budding structure through independent pathways and together they coalesce at the plasma membrane to form the mature virion.
journal_name
J Viroljournal_title
Journal of virologyauthors
Knipe DM,Baltimore D,Lodish HFdoi
10.1128/JVI.21.3.1128-1139.1977subject
Has Abstractpub_date
1977-03-01 00:00:00pages
1128-39issue
3eissn
0022-538Xissn
1098-5514journal_volume
21pub_type
杂志文章abstract::The mechanism of action of hepatitis B virus (HBV) X protein in transcriptional transactivation and in tumorigenesis remains obscure. We have used the yeast two-hybrid system to identify a cellular protein that can interact with HBV X protein. This protein, designated X-associated protein 1 (XAP-1), is a human homolog...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.2.1107-1114.1995
更新日期:1995-02-01 00:00:00
abstract::Our laboratory has previously reported isolation of human rhinovirus type 16 (HRV16) mutants which depend on WIN 52035 to grow. A rapid rise of progeny virus infectivity occurred when drug was added late in growth cycles, suggesting that the drug-dependence lesion was at the step of virus assembly (W. Wang et al., J. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.11.6235-6244.2003
更新日期:2003-06-01 00:00:00
abstract::The required activities of CD4(+) T cells and antibody against the virally encoded oncoprotein simian virus 40 (SV40) Tag have previously been demonstrated by our laboratory to be mediators in achieving antitumor responses and tumor protection through antibody-dependent cell-mediated cytotoxicity (ADCC). In this study...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01512-09
更新日期:2010-01-01 00:00:00
abstract::NF-κB-interacting long noncoding RNA (NKILA) was recently identified as a negative regulator of NF-κB signaling and plays an important role in the development of various cancers. It is well known that NF-κB-mediated activation of human immunodeficiency virus type 1 (HIV-1) long terminal repeat (LTR)-driven gene expres...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01057-20
更新日期:2020-08-17 00:00:00
abstract::We have examined the influence of the V1/V2 region of the human immunodeficiency virus type 1 (HIV-1) gp120 on certain biologic properties of the virus. We observed that on the genomic background of the T-cell-line-tropic strain, HIV-1SF2mc, both the V1 and V2 domains of the macrophage-tropic strain, HIV-1SF162mc, in ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.4.2253-2259.1994
更新日期:1994-04-01 00:00:00
abstract::Spontaneous deletion mutants of satellite bacteriophage P4 have been isolated and characterized. All of the deletions analyzed that were between 850 and 1,700 base pairs long are within the region nonessential for P4 lytic development; some of them cover the cII or the gop locus. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.54.1.233-235.1985
更新日期:1985-04-01 00:00:00
abstract::The Middle East respiratory syndrome coronavirus (MERS-CoV) nonstructural protein 16 (nsp16) is an S-adenosyl-l-methionine (SAM)-dependent 2'-O-methyltransferase (2'-O-MTase) that is thought to methylate the ribose 2'-OH of the first transcribed nucleotide (N1) of viral RNA cap structures. This 2'-O-MTase activity is ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02217-16
更新日期:2017-02-14 00:00:00
abstract::Resistance to human immunodeficiency virus type 1 protease (HIV PR) inhibitors results primarily from the selection of multiple mutations in the protease region. Because many of these mutations are selected for the ability to decrease inhibitor binding in the active site, they also affect substrate binding and potenti...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.3.1359-1368.2002
更新日期:2002-02-01 00:00:00
abstract::The human adenovirus type 2 (Ad2) mutant Ad2ts111 has previously been shown to contain two mutations which result in a complex phenotype. Ad2ts111 contains a single base change in the early region 1B (E1B) 19,000-molecular-weight (19K) coding region which yields a cyt deg phenotype and another defect which maps to the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.57.3.883-892.1986
更新日期:1986-03-01 00:00:00
abstract::Proteomic identification of human papillomavirus type 16 (HPV16) E6-interacting proteins revealed several proteins involved in ubiquitin-mediated proteolysis. In addition to the well-characterized E6AP ubiquitin-protein ligase, a second HECT domain protein (HERC2) and a deubiquitylating enzyme (USP15) were identified ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00605-09
更新日期:2009-09-01 00:00:00
abstract::The hemagglutinin of fowl plague virus has been expressed in Spodoptera frugiperda (Sf9) cells and in Estigmene acrea cells by using a baculovirus vector. Structural analysis revealed that the endo-H-resistant N-glycans of HA from Sf9 cells were predominantly trimannosyl core oligosaccharides, whereas in E. acrea cell...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.6.4103-4109.1996
更新日期:1996-06-01 00:00:00
abstract::Two hundred seventeen field isolates of cucumber mosaic cucumovirus (CMV), sampled from 11 natural populations, were typed by RNase protection assay (RPA) using probes from the genomic RNAs of strains in subgroup I and in subgroup II of CMV strains. Most (85%) of the analyzed isolates belonged to subgroup I. For these...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.2.934-940.1997
更新日期:1997-02-01 00:00:00
abstract::Human keratinocytes immortalized by full-length or early-region simian virus 40 (SV40) DNA grow in agarose and form tumors in nude mice, in contrast to keratinocytes immortalized by the E6/E7 genes of human papillomaviruses. To determine the molecular basis for this biological difference in growth, we have used the in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.21.10685-10691.2002
更新日期:2002-11-01 00:00:00
abstract::Chronic infections with viruses such as hepatitis B virus, hepatitis C virus, and HIV constitute a major global public health problem. Studies of chronic viral infections in humans and mice show that persistent antigenic stimulation induces dysregulation of T cell responses; virus-specific T cells either undergo clona...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00942-12
更新日期:2012-09-01 00:00:00
abstract::Although several virologic and immunologic factors associated with an increased risk of perinatal human immunodeficiency virus type 1 (HIV-1) transmission have been described, the mechanism of mother-to-child transmission is still unclear. More specifically, the question of whether selective pressures influence the tr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.5.3050-3057.2003
更新日期:2003-03-01 00:00:00
abstract::TMC125 is a potent new investigational nonnucleoside reverse transcriptase inhibitor (NNRTI) that is active against human immunodeficiency virus type 1 (HIV-1) with resistance to currently licensed NNRTIs. Sequential passage experiments with both wild-type virus and NNRTI-resistant virus were performed to identify mut...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.20.12773-12782.2005
更新日期:2005-10-01 00:00:00
abstract::The human immunodeficiency virus type 1 (HIV-1) Tat protein is essential for viral replication and stimulates transcription of the integrated provirus by recruiting the kinase complex TAK/P-TEFb, composed of cyclin T1 (CycT1) and Cdk9, to the viral TAR RNA element. TAK/P-TEFb phosphorylates the RNA polymerase II compl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.21.10579-10587.2002
更新日期:2002-11-01 00:00:00
abstract::We have generated hexon-modified adenovirus serotype 5 (Ad5) vectors that are not neutralized by Ad5-specific neutralizing antibodies in mice. These vectors are attractive for the advancement of vaccine products because of their potential for inducing robust antigen-specific immune responses in people with prior expos...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00462-13
更新日期:2013-09-01 00:00:00
abstract::A tumor line (58-2T) was established from a slowly growing tumor in a BALB/c mouse inoculated with M58-2 cells. The latter clonal cell line was isolated after bromodeoxyuridine treatment as a flat variant from nonproducer BALB/3T3 cells transformed by the Kirsten sarcoma virus. The 58-2T cells produced type C virus wi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.14.5.1262-1267.1974
更新日期:1974-11-01 00:00:00
abstract::The integrin alpha(v)beta3 has been shown to act as the receptor for internalization of foot-and-mouth disease virus (FMDV) (A12), with attachment being through a highly conserved RGD motif located on the G-H loop of viral capsid protein VP1. In addition, however, we have recently shown that efficient infection of cul...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.11.8357-8361.1997
更新日期:1997-11-01 00:00:00
abstract:UNLABELLED:Cell culture systems reproducing virus replication can serve as unique models for the discovery of novel bioactive molecules. Here, using a hepatitis C virus (HCV) cell culture system, we identified neoechinulin B (NeoB), a fungus-derived compound, as an inhibitor of the liver X receptor (LXR). NeoB was init...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00856-16
更新日期:2016-09-29 00:00:00
abstract::Gammaherpesviruses are important human and animal pathogens. Infection control has proven difficult because the key process of transmission is ill understood. Murid herpesvirus 4 (MuHV-4), a gammaherpesvirus of mice, is transmitted sexually. We show that this depends on the major virion envelope glycoprotein gp150. gp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00235-17
更新日期:2017-06-09 00:00:00
abstract::The lytic switch transactivator Zta initiates the ordered cascade of Epstein-Barr virus gene expression that culminates in virus production. Zta is a sequence-specific DNA-binding protein that transactivates early viral promotes via cis-acting sequences. Activation of some of these genes is mediated through binding to...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.7.4206-4212.1995
更新日期:1995-07-01 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) sequences were generated from blood and from brain tissue obtained by stereotactic biopsy from six patients undergoing a diagnostic neurosurgical procedure. Proviral DNA was directly amplified by nested PCR, and 8 to 36 clones from each sample were sequenced. Phylogenetic an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.11.7467-7481.1994
更新日期:1994-11-01 00:00:00
abstract::Purified preparations of human adenovirus type 12 showed two bands when subjected to isopycnic centrifugation in a density gradient of cesium chloride. Their density difference was about 0.003 g/ml, suggesting a small difference in their deoxyribonucleic acid to protein ratio. Virions with a lighter density can kill h...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.7.4.426-433.1971
更新日期:1971-04-01 00:00:00
abstract::The use of endocytic pathways by viral glycoproteins is thought to play various functions during viral infection. We previously showed in transfection assays that herpes simplex virus type 1 (HSV-1) glycoprotein B (gB) is transported from the cell surface back to the trans-Golgi network (TGN) and that two motifs of gB...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01231-07
更新日期:2007-12-01 00:00:00
abstract:UNLABELLED:Major histocompatibility complex class II (MHC-II) molecules play a central role in adaptive antiviral immunity by presenting viral peptides to CD4(+) T cells. Due to their key role in adaptive immunity, many viruses, including Kaposi's sarcoma-associated herpesvirus (KSHV), have evolved multiple strategies ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03713-14
更新日期:2015-05-01 00:00:00
abstract:UNLABELLED:Baculovirus DNAs are synthesized and inserted into preformed capsids to form nucleocapsids at a site in the infected cell nucleus, termed the virogenic stroma. Nucleocapsid assembly ofAutographa californicamultiple nucleopolyhedrovirus (AcMNPV) requires the major capsid protein VP39 and nine minor capsid pro...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02885-15
更新日期:2016-03-28 00:00:00
abstract::The 104-S monoclonal antibody immunoprecipitated from herpes simplex virus type 2 (HSV-2)-infected cell extracts the 75,000-molecular-weight glycoprotein gF and its 65,000-molecular-weight precursor (pgF). The precursor pgF was sensitive to endoglycosidase H digestion, indicating the presence of high mannose-type olig...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.47.1.185-192.1983
更新日期:1983-07-01 00:00:00
abstract::To study the variability of human immunodeficiency virus type 1 (HIV-1), we used immunotoxins to select for variants within a population of H9 cells persistently infected with a molecular clone of HIV-1 designated NL4-3. Chimeric immunotoxin CD4-PE40 (a chimeric fusion protein consisting of the amino-terminal two doma...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.1.75-81.1995
更新日期:1995-01-01 00:00:00