Abstract:
:Human keratinocytes immortalized by full-length or early-region simian virus 40 (SV40) DNA grow in agarose and form tumors in nude mice, in contrast to keratinocytes immortalized by the E6/E7 genes of human papillomaviruses. To determine the molecular basis for this biological difference in growth, we have used the individual SV40 oncogenes (large T antigen [LT] and small t antigen [st]) and human papillomavirus oncogenes (E6/E7) to study the progression of human epithelial cells from the nonimmortal to the immortal state as well as from the immortal to the anchorage-independent state. Transfection of primary human foreskin keratinocytes with LT did not immortalize cells but did extend the in vitro life span and produced cells that were resistant to calcium- and serum-induced terminal differentiation. Cells transfected with st alone did not passage beyond vector-transfected keratinocytes. The simultaneous expression of LT- and st-immortalized keratinocytes occurred without evidence of crisis and, as anticipated, these immortal cells were anchorage- independent for growth. Interestingly, we found that keratinocytes expressing both LT and st, but not keratinocytes with LT alone, exhibited increased phosphorylation of the protein kinase AKT. In addition, AKT activation was paralleled by an increase in telomerase activity. Addition of st to anchorage-dependent keratinocytes, expressing either LT (nonimmortal) or E6/E7 (immortal), converted the cells to anchorage independence, with similar accompanying increases in AKT phosphorylation and telomerase activity. However, it was not possible to induce keratinocyte growth in agarose with activated AKT and/or overexpressed hTERT, indicating that these newly defined st-induced activities are not sufficient for progression to the anchorage-independent state.
journal_name
J Viroljournal_title
Journal of virologyauthors
Yuan H,Veldman T,Rundell K,Schlegel Rdoi
10.1128/jvi.76.21.10685-10691.2002subject
Has Abstractpub_date
2002-11-01 00:00:00pages
10685-91issue
21eissn
0022-538Xissn
1098-5514journal_volume
76pub_type
杂志文章abstract::The envelope protein of human immunodeficiency virus type 1 HIV-1 undergoes proteolytic cleavage in the Golgi complex to produce subunits designated gp120 and gp41, which remain noncovalently associated. While gp41 has a well-characterized oligomeric structure, the maintenance of gp41-independent gp120 intersubunit co...
journal_title:Journal of virology
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doi:10.1128/jvi.74.10.4448-4455.2000
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abstract::Monomers of the genomic strand of hepatitis delta virus RNA were transcribed in vitro and then delivered to NIH 3T3 fibroblasts by using a liposome fusion technique. After 7 days, genome replication was detected, but only in fibroblasts that stably expressed the delta antigen. Sequence analysis of the replicated produ...
journal_title:Journal of virology
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doi:10.1128/JVI.64.6.3104-3107.1990
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.2.1109-1116.1996
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journal_title:Journal of virology
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doi:10.1128/JVI.61.3.921-924.1987
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.8.3525-3528.1989
更新日期:1989-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jvi.74.18.8234-8242.2000
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01051-08
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02016-17
更新日期:2018-04-27 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.2.10.1076-1080.1968
更新日期:1968-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01576-15
更新日期:2015-12-01 00:00:00
abstract::Myxomatosis is a recurrent problem on rabbit farms throughout Europe despite the success of vaccines. To identify gene variations of field and vaccine strains that may be responsible for changes in virulence, immunomodulation, and immunoprotection, the genomes of 6 myxoma virus (MYXV) strains were sequenced: German fi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01570-16
更新日期:2017-01-31 00:00:00
abstract::Radioactive DNA complementary to nucleotide sequences in Moloney murine sarcoma virus (MSV) and Moloney leukemia virus (M-MuLV) complex was made by the endogenous reverse transcriptase reaction. These virus stocks contained a threefold excess of MSV over M-MuLV as measured by biological assay. The complementary DNA wa...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.18.2.481-490.1976
更新日期:1976-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02343-08
更新日期:2009-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.9.5682-5684.1992
更新日期:1992-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.1.228-236.2000
更新日期:2000-01-01 00:00:00
abstract::There is a high degree of intraisolate sequence heterogeneity in the tax gene of human T-cell leukemia virus type I (HTLV-I), although the sequence variation between patients is small compared with that of human immunodeficiency virus type 1. In the present study, we investigated whether naturally occurring amino acid...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.4.2649-2653.1995
更新日期:1995-04-01 00:00:00
abstract::We studied the effect of filovirus infection on host cell gene expression by characterizing the regulation of gene expression responses in human liver cells infected with Zaire Ebolavirus (ZEBOV), Reston Ebolavirus (REBOV), and Marburgvirus (MARV), using transcriptional profiling and bioinformatics. Expression microar...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.6.3009-3020.2006
更新日期:2006-03-01 00:00:00
abstract::Infectious cDNA corresponding to the entire genome of the attenuated Sabin strain of type 1 poliovirus has been inserted into EcoRI site of bacterial plasmid pBR325. Two consecutive PstI fragments (nucleotide positions 1814 to 3421) of the infectious cDNA of the Sabin 1 strain were replaced by the corresponding DNA fr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.53.3.786-792.1985
更新日期:1985-03-01 00:00:00
abstract::Flavopiridol, roscovitine, and other inhibitors of Cyclin-Dependent Kinases (CDK) inhibit the replication of a variety of viruses in vitro while proving nontoxic in human clinical trials of their effects against cancer. Consequently, these and other Pharmacological CDK inhibitors (PCIs) have been proposed as potential...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.17.9352-9365.2004
更新日期:2004-09-01 00:00:00
abstract::The Coccolithoviridae are a group of viruses which infect the marine coccolithophorid microalga Emiliania huxleyi. The Emiliania huxleyi viruses (known as EhVs) described herein have 160- to 180-nm diameter icosahedral structures, have genomes of approximately 400 kbp, and consist of more than 450 predicted coding seq...
journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2012-03-01 00:00:00
abstract::The human papillomavirus type 11 (HPV-11) L1 major capsid protein can be trypsinized to generate recombinant capsomeres that retain HPV genotype-restricted capsid antigenicity (M. Li, T. P. Cripe, P. A. Estes, M. K. Lyon, R. C. Rose, and R. L. Garcea, J. Virol. 71:2988-2995, 1997). In the present study, HPV-11 virion-...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.7.6151-6154.1998
更新日期:1998-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01390-17
更新日期:2017-10-27 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00202-08
更新日期:2008-07-01 00:00:00
abstract::Effective, vaccine-induced CD8+ T-cell responses should recognize infected cells early enough to prevent production of progeny virions. We have recently shown that Gag-specific CD8+ T cells recognize simian immunodeficiency virus-infected cells at 2 h postinfection, whereas Env-specific CD8+ T cells do not recognize i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00926-07
更新日期:2007-11-01 00:00:00
abstract::A number of human immunodeficiency type 1 (HIV-1)-based vectors have recently been shown to transduce nondividing cells in vivo as well as in vitro. However, if these vectors are to be considered for eventual clinical use, a major consideration is to reduce the probability of unintended generation of replication-compe...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.3.1828-1834.1999
更新日期:1999-03-01 00:00:00