Abstract:
:The required activities of CD4(+) T cells and antibody against the virally encoded oncoprotein simian virus 40 (SV40) Tag have previously been demonstrated by our laboratory to be mediators in achieving antitumor responses and tumor protection through antibody-dependent cell-mediated cytotoxicity (ADCC). In this study, we further characterize the necessary immune cell components that lead to systemic tumor immunity within an experimental pulmonary metastatic model as the result of SV40 Tag immunization and antibody production. Immunized animals depleted of CD8(+) T cells at the onset of experimental tumor cell challenge developed lung tumor foci and had an overall decreased survival due to lung tumor burden, suggesting a role for CD8(+) T cells in the effector phase of the immune response. Lymphocytes and splenocytes harvested from SV40 Tag-immunized mice experimentally inoculated with tumor cells synthesized increased in vitro levels of the Th1 cytokine gamma interferon (IFN-gamma), as assessed by enzyme-linked immunosorbent assay (ELISA) and flow cytometry assays. CD8(+) T-cell activity was also heightened in SV40 Tag-immunized and tumor cell-challenged mice, based upon intracellular production of perforin, confirming the cytolytic properties of CD8(+) T cells against tumor cell challenge. Altogether, these data point to the role of recombinant SV40 Tag protein immunization in initiating a cytotoxic T-lymphocyte (CTL) response during tumor cell dissemination and growth. The downstream activity of CD8(+) T cells within this model is likely initiated from SV40 Tag-specific antibody mediating ADCC tumor cell destruction.
journal_name
J Viroljournal_title
Journal of virologyauthors
Lowe DB,Shearer MH,Jumper CA,Bright RK,Kennedy RCdoi
10.1128/JVI.01512-09subject
Has Abstractpub_date
2010-01-01 00:00:00pages
883-93issue
2eissn
0022-538Xissn
1098-5514pii
JVI.01512-09journal_volume
84pub_type
杂志文章abstract::Monoclonal antibodies produced against the prototype cell-adapted Wyoming strain of equine infectious anemia virus (EIAV), a lentivirus, were studied for reactivity with the homologous prototype and 16 heterologous isolates. Eighteen hybridomas producing monoclonal antibodies (MAbs) were isolated. Western blot (immuno...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.10.2956-2961.1987
更新日期:1987-10-01 00:00:00
abstract::Endogenous retroviruses, records of past retroviral infections, are ubiquitous in vertebrate genomes. On occasion, vertebrate hosts have co-opted retroviral genes for their own biological functions. Here, we perform a phylogenomic survey of retroviral gag gene homologs within vertebrate genomes and identify two ancien...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00542-19
更新日期:2019-06-28 00:00:00
abstract::Here, we investigated the pre-steady-state deoxynucleoside triphosphate (dNTP) incorporation kinetics of primate foamy virus (PFV) reverse transcriptase (RT) in comparison with those of HIV-1 and MuLV RTs. PFV RT displayed a drastic reduction in primer extension at low dNTP concentrations where HIV-1 RT remains highly...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00088-08
更新日期:2008-08-01 00:00:00
abstract::The severe acute respiratory syndrome coronavirus (SARS-CoV) nsp1 protein has unique biological functions that have not been described in the viral proteins of any RNA viruses; expressed SARS-CoV nsp1 protein has been found to suppress host gene expression by promoting host mRNA degradation and inhibiting translation....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02472-07
更新日期:2008-05-01 00:00:00
abstract::The presence of a high number of activated T cells in the bloodstream and spontaneous proliferation of peripheral blood mononuclear cells in vitro are striking characteristics of human T-cell leukemia virus type I (HTLV-I) infection. The HTLV-I regulatory protein Tax and the envelope protein gp46 have been implicated ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.6.3134-3141.1993
更新日期:1993-06-01 00:00:00
abstract:UNLABELLED:Phosphoinositides and phosphoinositide binding proteins play a critical role in membrane and protein trafficking in eukaryotes. Their critical role in replication of cytoplasmic viruses has just begun to be understood. Poxviruses, a family of large cytoplasmic DNA viruses, rely on the intracellular membranes...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03073-14
更新日期:2015-02-01 00:00:00
abstract::The presence of complement-fixing (CF) antibody reactive with T antigen and with viral C antigen in hamsters bearing adenovirus 12-induced tumors has been confirmed. Antibody activity in serum obtained at a time when the host was bearing large tumors was found to be associated exclusively with 7S immunoglobulins. Two ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.1.4.678-683.1967
更新日期:1967-08-01 00:00:00
abstract::Adenine residues of 70S avian myeloblastosis virus (AMV) RNA are modified when reacted with chloroacetaldehyde. This modification introduces characteristic fluorescent epsilon-adenosine (epsilonA) probes which were used to monitor the reaction. Under suitable conditions, modified 70S(epsilonA) RNA was maintained intac...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.14.5.1108-1114.1974
更新日期:1974-11-01 00:00:00
abstract::The latency-related (LR) gene of bovine herpesvirus 1 (BHV-1) is abundantly expressed during latency. A mutant BHV-1 strain that contains three stop codons at the 5' terminus of the LR gene (LR mutant) does not reactivate from latency. This study demonstrates that the LR mutant does not express open reading frame 2 or...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.6.3184-3189.2004
更新日期:2004-03-01 00:00:00
abstract::PCR amplification of baboon endogenous virus (BaEV) long terminal repeat, reverse transcriptase gene, and env fragments from 24 different species of African monkeys indicates that BaEV is less widespread than was formerly thought. Instead of being present in every species of African primates, BaEV can be found only in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.12.7877-7887.1995
更新日期:1995-12-01 00:00:00
abstract::The internal deletion mutant (DI-LT) derived from the heat-resistant strain of vesicular stomatitis virus synthesizes an aberrant polyadenylated mRNA (G*) containing a transcript of the partially deleted polymerase gene covalently linked to the 3' end of the glycoprotein message (R. C. Herman and R. A. Lazzarini, J. V...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.46.3.709-717.1983
更新日期:1983-06-01 00:00:00
abstract::Radioactive DNA complementary to nucleotide sequences in Moloney murine sarcoma virus (MSV) and Moloney leukemia virus (M-MuLV) complex was made by the endogenous reverse transcriptase reaction. These virus stocks contained a threefold excess of MSV over M-MuLV as measured by biological assay. The complementary DNA wa...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.18.2.481-490.1976
更新日期:1976-05-01 00:00:00
abstract::The pregenomic RNA (pgRNA) of hepatitis B virus (HBV) serves as the messenger for both core and P proteins, with the downstream P gene translated by ribosomal leaky scanning. HBV replication begins with packaging of the pgRNA and P protein into core protein particles, followed by conversion of RNA into DNA. Genotype G...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05583-11
更新日期:2011-12-01 00:00:00
abstract::The NS3 protein of hepatitis C virus (HCV) possesses protease activity responsible for the proteolytic cleavage of the viral polyprotein at the junctions of nonstructural proteins downstream of NS3. The NS3 protein was also found to be internally cleaved. In this study, we demonstrated that internal cleavages occurred...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00348-07
更新日期:2007-08-01 00:00:00
abstract::The mucosal adjuvant effect of synthetic double-stranded RNA polyriboinosinic polyribocytidylic acid [poly(I:C)] against influenza virus was examined under intranasal coadministration with inactivated hemagglutinin (HA) vaccine in BALB/c mice and was shown to have a protective effect against both nasal-restricted infe...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.5.2910-2919.2005
更新日期:2005-03-01 00:00:00
abstract::The V protein of Sendai virus (SeV) is nonessential to virus replication in cell culture but indispensable to viral pathogenicity in mice. The highly conserved cysteine-rich zinc finger-like domain in its carboxyl terminus is believed to be responsible for this viral pathogenicity. In the present study, we showed that...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.17.7834-7841.2000
更新日期:2000-09-01 00:00:00
abstract::The various strains of BK virus (BKV) exhibit a remarkable degree of heterogeneity in the transcriptional control region, which may affect the biological characteristics of a BKV strain. We describe the detection and sequencing of BKV control regions directly from urine samples and after propagation in cell culture. A...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.8.3864-3871.1990
更新日期:1990-08-01 00:00:00
abstract::Nef is a regulatory gene product of human immunodeficiency virus type 1 (HIV-1) and other primate lentiviruses which enhances virion infectivity by an unknown mechanism. We report here that Nef is detectable at moderate levels in preparations of HIV-1 virions which lack active viral protease (PR). Significantly smalle...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.2.1013-1018.1997
更新日期:1997-02-01 00:00:00
abstract::The molecular mechanisms responsible for random integration and gene targeting by recombinant adeno-associated virus (AAV) vectors are largely unknown, and whether vectors derived from autonomous parvoviruses transduce cells by similar pathways has not been investigated. In this report, we constructed vectors based on...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.24.13136-13145.2003
更新日期:2003-12-01 00:00:00
abstract::We investigated the integration sites, infectivities, and expression of Rous-associated virus-0 (Rav-0) DNAs in exogenously infected turkey and chicken cells. Restriction endonuclease analyses of the DNAs of RAV-0-infected cells indicated that unique integration sites of RAV-0 DNA were detectable in clones of RAV-0-in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.36.3.684-691.1980
更新日期:1980-12-01 00:00:00
abstract::Poliovirus infectious RNA can be synthesized in vitro using phage DNA-dependent RNA-polymerases. These synthetic transcripts contain several extra nucleotides at the 5' end, which are deleted during replication to generate authentic viral genomes. We removed those 5'-end extra nucleotides utilizing a hammerhead ribozy...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.14.6394-6400.2000
更新日期:2000-07-01 00:00:00
abstract::Due to increasing concerns about human infection by various H7 influenza viruses, including recent H7N9 viruses, we evaluated the genetic relationships and cross-protective efficacies of three different Eurasian H7 avian influenza viruses. Phylogenic and molecular analyses revealed that recent Eurasian H7 viruses can ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02259-16
更新日期:2017-05-12 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) efficiently enters cells of Old World monkeys but encounters a block before reverse transcription. This restriction is mediated by a dominant repressive factor. Recently, a member of the tripartite motif (TRIM) family proteins, TRIM5alpha, was identified as a blocking factor...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.14.8870-8877.2005
更新日期:2005-07-01 00:00:00
abstract::Adeno-associated viruses (AAVs) are small single-stranded DNA viruses that can package and deliver nongenomic DNA for therapeutic gene delivery. AAV8, a liver-tropic vector, has shown great promise for the treatment of hemophilia A and B. However, as with other AAV vectors, host anti-capsid immune responses are a dete...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00218-12
更新日期:2012-08-01 00:00:00
abstract::Phosphorylation of viral proteins plays important roles in the influenza A virus (IAV) life cycle. By using mass spectrometry, we identified tyrosine 132 (Y132) as a phosphorylation site of the matrix protein (M1) of the influenza virus A/WSN/1933(H1N1). Phosphorylation at this site is essential to the process of viru...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03024-12
更新日期:2013-06-01 00:00:00
abstract::In vitro replication of coxsackievirus B3 (CVB3) in cells of the immune system derived from uninfected adolescent A/J and C57BL/6J mice and replication of CVB3 in and association with immune cells from spleens of infected animals in vivo were assessed. Nonstimulated or mitogen-stimulated spleen cells were minimally pe...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.7.4632-4645.1996
更新日期:1996-07-01 00:00:00
abstract::HIV-1 circulates within an infected host as a genetically heterogeneous viral population. Viral intrahost diversity is shaped by substitutional evolution and recombination. Although many studies have speculated that recombination could have a significant impact on viral phenotype, this has never been definitively demo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00131-11
更新日期:2011-06-01 00:00:00
abstract::We previously reported that nucleolin, a representative nucleolar marker, interacts with nonstructural protein 5B (NS5B) of hepatitis C virus (HCV) through two independent regions of NS5B, amino acids 208 to 214 and 500 to 506. We also showed that truncated nucleolin that harbors the NS5B-binding region inhibited the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.7.3332-3340.2006
更新日期:2006-04-01 00:00:00
abstract::Translation of aphthovirus RNA is initiated at an internal ribosome entry site (IRES) element, preceding the first functional AUG initiation codon. The effect of mutations at the base of domain 3 of the aphthovirus IRES on translation activity has been analyzed by site-directed mutagenesis and expression of bicistroni...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.2.992-998.1996
更新日期:1996-02-01 00:00:00
abstract::Recombination is one of the driving forces of viral evolution. RNA recombination events among similar RNA viruses are frequent, although RNA recombination could also take place among unrelated viruses. In this paper, we have established efficient interviral recombination systems based on yeast and plants. We show that...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01015-19
更新日期:2019-12-12 00:00:00