Abstract:
:We investigated the cis-acting sequences involved in termination of vesicular stomatis virus mRNA synthesis by using bicistronic genomic analogs. All of the cis-acting signals necessary for termination reside within the first 13 nucleotides of the 23-nucleotide conserved gene junction. This 13-nucleotide termination sequence at the end of the upstream gene comprises the tetranucleotide AUAC, the tract containing seven uridines (U7 tract), and the intergenic dinucleotide (GA), but it does not include the downstream gene start sequence. Data presented here show that upstream mRNA termination is independent of downstream mRNA initiation. Alteration of any nucleotide in the 13-nucleotide sequence decreased the termination activity of the gene junction and resulted in increased synthesis of a bicistronic readthrough RNA. This finding indicated that the wild-type gene junction has evolved to achieve the maximum termination efficiency. The most critical position of the AUAC sequence was the C, which could not be altered without complete loss of mRNA termination. Reducing the length of the wild-type U7 tract to zero, five, or six U residues also totally abolished mRNA termination, resulting in exclusive synthesis of the bicistronic readthrough mRNA. Shortening the wild-type U7 tract to either five or six U residues abolished VSV polymerase slippage during readthrough RNA synthesis. Since neither the U5 nor U6 template was able to direct mRNA termination, these data imply that polymerase slippage is a prerequisite for termination. Evidence is also presented to show that in addition to causing polymerase slippage, the U7 tract itself or its poly(A) product constitutes an essential signal for mRNA termination.
journal_name
J Viroljournal_title
Journal of virologyauthors
Barr JN,Whelan SP,Wertz GWdoi
10.1128/JVI.71.11.8718-8725.1997subject
Has Abstractpub_date
1997-11-01 00:00:00pages
8718-25issue
11eissn
0022-538Xissn
1098-5514journal_volume
71pub_type
杂志文章abstract::A temperature-sensitive mutant of adenovirus 31 was defective in formation of the DNA replication complex, suggesting the existence of a virus-coded protein necessary for the complex-formation. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.2.538-540.1974
更新日期:1974-02-01 00:00:00
abstract::Nonhuman primates are widely used to study correlates of protective immunity in AIDS research. Successful cellular immune responses have been difficult to identify because heterogeneity within macaque major histocompatibility complex (MHC) genes results in quantitative and qualitative differences in immune responses. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01841-06
更新日期:2007-01-01 00:00:00
abstract::Thogoto virus (THOV) is a tick-transmitted orthomyxovirus with a genome consisting of six negative-stranded RNA segments. To rescue a recombinant THOV, the viral structural proteins were produced from expression plasmids by means of a vaccinia virus expressing the T7 RNA polymerase. Genomic virus RNAs (vRNAs) were gen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.19.9282-9286.2001
更新日期:2001-10-01 00:00:00
abstract::On the basis of the conservation of neuraminidase (N) active-site residues in influenza virus N and paramyxovirus hemagglutinin-neuraminidase (HN), it has been suggested that the three-dimensional (3D) structures of the globular heads of the two proteins are broadly similar. In this study, details of this structural s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.8.6155-6167.1997
更新日期:1997-08-01 00:00:00
abstract::Whole inactivated virus vaccines from the FL4 cell line protected against challenge with homologous feline immunodeficiency virus (Petaluma strain) but not against a heterologous FIV isolate (GL-8) which is distinct from the Petaluma strain in virus neutralization. Protection was associated with a type-specific neutra...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.2.1253-1255.1995
更新日期:1995-02-01 00:00:00
abstract::HIV-1 infectivity is strongly restricted by TRIM5α from certain primate species but has been described as being only marginally susceptible to human TRIM5α. In this study, we evaluated the effects of the modulation of human TRIM5α activity (pretreatment of target cells with alpha interferon, expression of a pre-miRNA ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00758-10
更新日期:2010-11-01 00:00:00
abstract::Retroviral Gag proteins have the ability to induce budding and particle release from the plasma membrane when expressed in the absence of all of the other virus-encoded components; however, the locations of the functional domains within the Gag protein that are important for this process are poorly understood. It was ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.9.5550-5561.1993
更新日期:1993-09-01 00:00:00
abstract::Wild-type human respiratory syncytial virus (HRSV) is a poor inducer of alpha/beta interferons (IFN-alpha/beta). However, recombinant HRSV lacking the NS1 and NS2 genes (Delta NS1/2) induced high levels of IFN-alpha and -beta in human pulmonary epithelial cells (A549) as well as in macrophages derived from primary hum...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.8.4363-4369.2004
更新日期:2004-04-01 00:00:00
abstract::Binding of the human immunodeficiency virus type 1 (HIV-1) Gag protein precursor, Pr55(Gag), to membrane is an indispensable step in virus assembly. Previously, we reported that a matrix (MA) residue 6 substitution (6VR) imposed a virus assembly defect similar to that observed with myristylation-defective mutants, sug...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.5.4136-4144.1999
更新日期:1999-05-01 00:00:00
abstract::All herpesviruses have a layer of protein called the tegument that lies between the virion membrane and the capsid. The tegument consists of multiple, virus-encoded protein species that together can account for nearly half the total virus protein. To clarify the structure of the tegument and its attachment to the caps...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00777-09
更新日期:2009-08-01 00:00:00
abstract::Six phages were isolated from sewage and from a lysogenic strain. Three of them, belonging to a new morphological group, had contractile tails and elongated heads with axial ratios of 2.4:1. Two phages, possessing short tails and very long heads with axial ratios of 3.5:1, were new isolates of an extremely rare group....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.3.706-711.1974
更新日期:1974-03-01 00:00:00
abstract::Porcine circovirus 2 (PCV2) is a T=1 nonenveloped icosahedral virus that has had severe impact on the swine industry. Here we report the crystal structure of an N-terminally truncated PCV2 virus-like particle at 2.3-Å resolution, and the cryo-electron microscopy (cryo-EM) image reconstruction of a full-length PCV2 vir...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00737-11
更新日期:2011-08-01 00:00:00
abstract::Xenotransplantation of porcine organs carries the risk of reactivation of latent virus in donor and recipient tissues as well as transmission of viruses between species. We have investigated the activation of baboon cytomegalovirus (BCMV) and porcine CMV (PCMV) in a pig-to-primate model of xenotransplantation. Tissues...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.10.4734-4740.2002
更新日期:2002-05-01 00:00:00
abstract::The secreted cytokine alpha/beta interferon (IFN-alpha/beta) binds its receptor to activate the Jak-STAT signal transduction pathway, leading to formation of the heterotrimeric IFN-stimulated gene factor 3 (ISGF3) transcription complex for induction of IFN-stimulated genes (ISGs) and establishment of an antiviral stat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01787-08
更新日期:2009-03-01 00:00:00
abstract::Influenza B virus (IBV) is considered a major human pathogen, responsible for seasonal epidemics of acute respiratory illness. Two antigenically distinct IBV hemagglutinin (HA) lineages cocirculate worldwide with little cross-reactivity. Live attenuated influenza virus (LAIV) vaccines have been shown to provide better...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00056-17
更新日期:2017-05-26 00:00:00
abstract::We have detected a mouse mammary tumor virus (MMTV)-specific 1.7-kilobase (kb) polyadenylated RNA in mammary glands of several mouse strains. In BALB/c mice, it is the only MMTV-specific RNA species present. C3H and GR mammary glands and tumors contain, in addition, 3.8- and 7.8-kb MMTV RNAs. Nuclease S1 analysis was ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.46.2.362-370.1983
更新日期:1983-05-01 00:00:00
abstract::Glycoprotein gIII of pseudorabies virus is a major antigen found in the envelopes of virus particles as well as in and on the surfaces of infected cells. It is not an essential gene product for virus growth in tissue culture. In this report, we provide evidence that, although it is not essential, the gIII protein is r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.7.2512-2515.1988
更新日期:1988-07-01 00:00:00
abstract::We tested chemokine receptor subset usage by diverse, well-characterized primary viruses isolated from peripheral blood by monitoring viral replication with CCR1, CCR2b, CCR3, CCR5, and CXCR4 U87MG.CD4 transformed cell lines and STRL33/BONZO/TYMSTR and GPR15/BOB HOS.CD4 transformed cell lines. Primary viruses were iso...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.11.9307-9312.1998
更新日期:1998-11-01 00:00:00
abstract::Influenza virus enters its host cell by receptor-mediated endocytosis followed by acid-activated membrane fusion in endosomes. The viral ribonucleoprotein particles (vRNPs) delivered into the cytosol then dissociate from the matrix protein, M1, and from each other, after which they are individually imported into the n...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.12.8391-8401.1996
更新日期:1996-12-01 00:00:00
abstract::Open reading frame 24 (ORF24) of murine gammaherpesvirus 68 (MHV-68) is conserved among beta- and gammaherpesviruses; however, its function in viral replication has not been defined. Using MHV-68 as a model, we have identified ORF24 as being essential for viral replication. An ORF24-null virus was generated and shown ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02726-06
更新日期:2007-06-01 00:00:00
abstract::Poorly cytopathic or noncytopathic viruses can escape immune surveillance and establish a chronic infection. Here we exploited the strategy of combining antiviral drug treatment with the induction of a neutralizing antibody response to avoid the appearance of neutralization-resistant virus variants. Despite the fact t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.13.5896-5901.2000
更新日期:2000-07-01 00:00:00
abstract::We have tested triple and quadruple combinations of human monoclonal antibodies (MAbs), which are directed against various epitopes on human immunodeficiency virus type 1 (HIV-1) envelope glycoproteins, and a high-titer anti-HIV-1 human immunoglobulin (HIVIG) preparation for their abilities to neutralize a chimeric si...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.4.3235-3240.1998
更新日期:1998-04-01 00:00:00
abstract::After herpes simplex virus infection of hamster kidney cells there is an induction of nucleoside phosphotransferase activity which can utilize AMP as phosphate donor. The activity is immunologically specific for the infected cell and is induced concomitantly with the virus-coded pyrimidine deoxynucleoside kinase activ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.17.3.1056-1059.1976
更新日期:1976-03-01 00:00:00
abstract::The alphaherpesvirus proteins UL31 and UL34 and their homologues in other herpesvirus subfamilies cooperate at the nuclear membrane in the export of nascent herpesvirus capsids. We studied the respective betaherpesvirus proteins M53 and M50 in mouse cytomegalovirus (MCMV). Recently, we established a random approach to...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00681-10
更新日期:2010-09-01 00:00:00
abstract::Using coimmunoprecipitation and glutathione S-transferase pulldown experiments, we found that polyomavirus large T antigen binds to p300 in vivo and in vitro. The N-terminal region of the viral protein, including the pRB binding motif, was dispensable for this interaction, which involved several regions within the C-t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.2.1734-1739.1999
更新日期:1999-02-01 00:00:00
abstract::The nef gene contributes to the replication of primate lentiviruses by altering the trafficking of cellular proteins involved in adaptive immunity (class I and II major histocompatibility complex [MHC]) and viral transmission (CD4 and DC-SIGN). A conserved acidic leucine-based sequence (E(160)xxxLL) within human immun...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.4.1837-1849.2006
更新日期:2006-02-01 00:00:00
abstract:UNLABELLED:Development of a vaccine to prevent congenital cytomegalovirus infection is a major public health priority. Live vaccines attenuated through mutations targeting viral mechanisms responsible for evasion of host defense may be both safe and efficacious. Safety and vaccine efficacy were evaluated using a guinea...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01419-15
更新日期:2015-10-01 00:00:00
abstract:UNLABELLED:Enterovirus A71 (EV-A71) is a major cause of hand, foot, and mouth disease (HFMD) and is particularly prevalent in parts of Southeast Asia, affecting thousands of children and infants each year. Revealing the evolutionary and epidemiological dynamics of EV-A71 through time and space is central to understandi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00706-15
更新日期:2015-09-01 00:00:00
abstract::The immune response to a synthetic peptide, H3 HA1(305-328), representing the C'-terminal 24 amino acid residues of the HA1 chain of the hemagglutinin of the H3 subtype of influenza virus is controlled by genes in the I region of the major histocompatibility complex. Mice of the H-2d haplotype are high responders and ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.5.1746-1752.1988
更新日期:1988-05-01 00:00:00
abstract::During the latent phase, Kaposi's sarcoma-associated herpes virus (KSHV) maintains itself inside the host by escaping the host immune surveillance mechanism through restricted protein expression. Latency-associated nuclear antigen (LANA), the most abundantly expressed protein, is essential for viral persistence, as it...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01508-19
更新日期:2020-01-17 00:00:00