Abstract:
:Antibodies present in two peritoneal exudates of rats bearing abdominal tumors induced by UR2-transformed rat cells were characterized. The ability to immunoprecipitate p68gag-ros and to inhibit the protein and phospholipid kinase activities of this protein was investigated. One of the exudates specifically inhibited tyrosyl phosphorylation by p68gag-ros but not the activity of other known tyrosyl kinases, such as p150gag-fps of UR1 avian sarcoma virus, p60src, and the insulin receptor. It precipitated p68gag-ros but not Pr76 or other gag-related proteins from UR2-infected cells. Phosphorylation of phosphatidylinositol was not affected by this exudate, suggesting that this activity is not intrinsic to p68gag-ros. Another exudate precipitated p68gag-ros but not gag-related proteins from UR2-infected cells or p140gag-fps from Fujinami sarcoma virus-infected cells. These results demonstrated that the antibodies in these exudates recognized epitopes present in the ros portion of the fused protein p68gag-ros, but only one of the two exudates inhibited the intrinsic tyrosyl kinase of p68gag-ros.
journal_name
J Viroljournal_title
Journal of virologyauthors
Balduzzi PC,Chovav M,Christensen JR,Macara IGdoi
10.1128/JVI.60.2.765-767.1986subject
Has Abstractpub_date
1986-11-01 00:00:00pages
765-7issue
2eissn
0022-538Xissn
1098-5514journal_volume
60pub_type
杂志文章abstract::Murine gammaherpesvirus 68 (gammaHV68) provides an important experimental model for understanding mechanisms of immune control of the latent human gammaherpesviruses. Antiviral CD8 T cells play a key role throughout three separate phases of the infection: clearance of lytic virus, control of the latency amplification ...
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journal_title:Journal of virology
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doi:10.1128/JVI.71.10.7903-7910.1997
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journal_title:Journal of virology
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.8.4994-5010.1995
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.9.3281-3287.1988
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.12.9803-9807.1997
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doi:10.1128/JVI.70.4.2508-2515.1996
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journal_title:Journal of virology
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pub_type: 杂志文章
doi:10.1128/jvi.74.13.6068-6076.2000
更新日期:2000-07-01 00:00:00
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更新日期:2006-08-01 00:00:00