Abstract:
:The E7 proteins of human papillomaviruses (HPVs) promote S-phase reentry in differentiated keratinocytes of the squamous epithelia to support viral DNA amplification. In this study, we showed that nuclear p130 was present in the differentiated strata of several native squamous epithelia susceptible to HPV infection. In contrast, p130 was below the level of detection in HPV-infected patient specimens. In submerged and organotypic cultures of primary human keratinocytes, the E7 proteins of the high-risk mucosotrophic HPV-18, the benign cutaneous HPV-1, and, to a lesser extent, the low-risk mucosotropic HPV-11 destabilized p130. This E7 activity depends on an intact pocket protein binding domain and a casein kinase II (CKII) phosphorylation motif. Coimmunoprecipitation experiments showed that both E7 domains were important for binding to p130 in extracts of organotypic cultures. Metabolic labeling in vivo demonstrated that E7 proteins were indeed phosphorylated in a CKII motif-dependent manner. Moreover, the efficiencies of the E7 proteins of various HPV types or mutations to induce S-phase reentry in spinous cells correlated with their relative abilities to bind and to destabilize p130. Collectively, these data support the notion that p130 controls the homeostasis of the differentiated keratinocytes and is therefore targeted by E7 for degradation to establish conditions permissive for viral DNA amplification.
journal_name
J Viroljournal_title
Journal of virologyauthors
Genovese NJ,Banerjee NS,Broker TR,Chow LTdoi
10.1128/JVI.01202-07subject
Has Abstractpub_date
2008-05-01 00:00:00pages
4862-73issue
10eissn
0022-538Xissn
1098-5514pii
JVI.01202-07journal_volume
82pub_type
杂志文章abstract::Several lines of evidence suggest that various cofactors may be required for prion replication. PrP binds to polyanions, and RNAs were shown to promote the conversion of PrP(C) into PrP(Sc) in vitro. In the present study, we investigated strain-specific differences in RNA requirement during in vitro conversion and the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01286-12
更新日期:2012-10-01 00:00:00
abstract::Reverse transcription in hepatitis B viruses is initiated through a unique protein priming mechanism whereby the viral reverse transcriptase (RT) first assembles into a ribonucleoprotein (RNP) complex with its RNA template and then initiates DNA synthesis de novo using the RT itself as a protein primer. RNP formation ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.24.11447-11455.2000
更新日期:2000-12-01 00:00:00
abstract::A molecular clone of mouse-neuroadapted yellow fever 17D virus (SPYF-MN) was used to identify critical determinants of viral neuroinvasiveness in a SCID mouse model. Virus derived from this clone differs from nonneuroinvasive YF5.2iv virus at 29 nucleotide positions, encoding 13 predicted amino acid substitutions and ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.22.12232-12242.2003
更新日期:2003-11-01 00:00:00
abstract::TT virus (TTV) is a recently identified widespread DNA virus of humans that produces persistent viremia in the absence of overt clinical manifestations. In an attempt to shed light on the dynamics of chronic infection, we measured the levels of TTV in the plasma of 25 persistently infected patients during the first 3 ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.24.11999-12004.2001
更新日期:2001-12-01 00:00:00
abstract::Severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) has caused a pandemic of historic proportions and continues to spread globally, with enormous consequences to human health. Currently there is no vaccine, effective therapeutic, or prophylactic. As with other betacoronaviruses, attachment and entry o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01987-20
更新日期:2021-01-13 00:00:00
abstract::We have previously described a nonconditional mutant of avian sarcoma virus (SE21Q1b) which fails to package viral RNA (Gallis et al., Virology 94:146-161, 1979; Linial et al., Cell 15:1371-1381, 1978). Quail cells transformed by SE21Q1b contain normal amounts of intracellular viral mRNA's for src, env, and gag-pol an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.36.2.450-456.1980
更新日期:1980-11-01 00:00:00
abstract::We have used immunofluorescence in parallel with transmission and scanning electron microscopy to characterize the unusual cytoplasmic and nucleolar accumulation of Simian virus 40 (SV40) virion protein (C antigen) at restrictive temperatures (39 to 41 C) in monkey cells infected with a temperature-sensitive mutant of...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.14.6.1530-1546.1974
更新日期:1974-12-01 00:00:00
abstract::Varicella-zoster virus (VZV), an alphaherpesvirus restricted to humans, infects differentiated cells in vivo, including T lymphocytes, keratinocytes, and neurons, and spreads rapidly in confluent cultured dermal fibroblasts (HFFs). In VZV-infected HFFs, atypical expression of cyclins D3 and B1 occurs along with the in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00153-08
更新日期:2008-12-01 00:00:00
abstract::Human cytomegalovirus (HCMV) is the most common congenitally transmitted pathogen worldwide, impacting an estimated 1 million newborns annually. Congenital HCMV (cCMV) infection is a major global contributor to long-term neurologic deficits, including deafness, microcephaly, and neurodevelopmental delay, as well as to...
journal_title:Journal of virology
pub_type: 杂志文章,评审
doi:10.1128/JVI.00030-18
更新日期:2018-03-14 00:00:00
abstract::Herpes simplex virus type 1 (HSV-1) B capsids are composed of seven proteins, designated VP5, VP19C, 21, 22a, VP23, VP24, and VP26 in order of decreasing molecular weight. Three proteins (21, 22a, and VP24) are encoded by a single open reading frame (ORF), UL26, and include a protease whose structure and function have...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.9.5365-5374.1994
更新日期:1994-09-01 00:00:00
abstract::The specificity of herpes simplex virus type 1-specific cytotoxic T cells was examined with target cells expressing either input viral structural antigens or antigens resulting from permissive infection or cells from an interrupted infection in which they expressed predominantly nonstructural immediate-early proteins....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.7.2265-2273.1988
更新日期:1988-07-01 00:00:00
abstract::Human adenoviruses typically cause mild infections in the upper or lower respiratory tract, gastrointestinal tract, or ocular epithelium. However, adenoviruses may be life-threatening in patients with impaired immunity and some serotypes cause epidemic outbreaks. Attachment to host cell receptors activates cell signal...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02014-12
更新日期:2013-02-01 00:00:00
abstract::An immediate-early protein of murine cytomegalovirus (MCMV), pp89, elicits an immunodominant and protective major histocompatibility complex (MHC) class I Ld-restricted CD8+ T-lymphocyte response. Remarkably, presentation of the naturally processed peptide of pp89, the nonapeptide YPHFMPTNL, is abolished during permis...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.1.289-297.1994
更新日期:1994-01-01 00:00:00
abstract::Hepatitis C virus (HCV) is an important human pathogen associated with chronic liver disease. Recently, based on a genotype 2a isolate, tissue culture systems supporting complete replication and infectious virus production have been developed. In this study, we used cell culture-produced infectious HCV to analyze the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.4.1734-1741.2006
更新日期:2006-02-01 00:00:00
abstract::Using a recently developed model for in vitro generation of pp65-positive polymorphonuclear leukocytes (PMNLs), we demonstrated that PMNLs from immunocompetent subjects may harbor both infectious human cytomegalovirus (HCMV) and viral products (pp65, p72, DNA, and immediate-early [IE] and pp67 late mRNAs) as early as ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.12.5629-5638.2000
更新日期:2000-06-01 00:00:00
abstract:UNLABELLED:The emergence of Middle East respiratory syndrome-coronavirus (MERS-CoV) in the Middle East since 2012 has caused more than 900 human infections with ∼40% mortality to date. Animal models are needed for studying pathogenesis and for development of preventive and therapeutic agents against MERS-CoV infection....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03427-14
更新日期:2015-04-01 00:00:00
abstract::Acute arenavirus disease in primates, like Lassa hemorrhagic fever in humans, begins with flu-like symptoms and leads to death approximately 2 weeks after infection. Our goal was to identify molecular changes in blood that are related to disease progression. Rhesus macaques (Macaca mulatta) infected intravenously with...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00536-07
更新日期:2007-08-01 00:00:00
abstract::Human T-cell leukemia virus type 1 (HTLV-1) is the etiologic agent of adult T-cell leukemia/lymphoma and is associated with a variety of immunoregulatory disorders. HTLV-1 has been shown to bind to and infect a variety of hematopoietic and nonhematopoietic cells. However, both in vivo and in vitro, the provirus is mos...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.4.2101-2106.1996
更新日期:1996-04-01 00:00:00
abstract::The route of internalization of the pestivirus bovine viral diarrhea virus (BVDV) was studied by using different chemical and biophysical inhibitors of endocytosis. Expression of the dominant-negative mutant Dyn(K44A) of the GTPase dynamin in MDBK cells, as well as the treatment of the cells with chlorpromazine and be...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.7.4191-4200.2005
更新日期:2005-04-01 00:00:00
abstract::Adenovirus class B (Ad3 and Ad7) and class C (Ad1, Ad5, and Ad6) late r-strand mRNA's were found to have segmented 5' leaders. These leaders were very similar among serotype within a class but differed in sequence from the leaders on late mRNA's of a different class. However, the leader components of class B viruses m...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.30.3.899-912.1979
更新日期:1979-06-01 00:00:00
abstract::Type 3 reovirus inhibits L cell DNA synthesis, whereas type 1 reovirus exerts little or no effect on L cell DNA synthesis. By using recombinant viruses containing both type 1 and type 3 double-standard RNA segments, we determined that one double-stranded RNA segment, the reovirus type 3 S1 double-stranded RNA segment ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.38.1.389-392.1981
更新日期:1981-04-01 00:00:00
abstract::The immunosuppressant drug rapamycin blocks progression of the cell cycle at G1 in mammalian cells and yeast. We recently showed that rapamycin inhibits both in vitro and in vivo cap-dependent, but not cap-independent, translation. This inhibition is causally related to reduced phosphorylation and consequent activatio...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.12.8993-8996.1996
更新日期:1996-12-01 00:00:00
abstract::We are using site-directed mutagenesis of single viral genes to identify and analyze the genetic determinants of human and simian immunodeficiency virus pathogenicity. In a first approach, we have constructed a series of simian immunodeficiency virus SIVmac nef mutants by partial deletion and insertions in the nef gen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.10.5237-5243.1991
更新日期:1991-10-01 00:00:00
abstract::The major phosphoprotein common to woolly monkey sarcoma virus, gibbon ape lymphosarcoma virus, and type C viruses of the lower mammalian species (mouse, rat, cat), with the exception of the endogenous cat virus (RD-114), is the polypeptide of about 12,000 molecular weight. The protein-phosphate bond in this polypepti...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.16.1.123-131.1975
更新日期:1975-07-01 00:00:00
abstract::PCR amplification of baboon endogenous virus (BaEV) long terminal repeat, reverse transcriptase gene, and env fragments from 24 different species of African monkeys indicates that BaEV is less widespread than was formerly thought. Instead of being present in every species of African primates, BaEV can be found only in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.12.7877-7887.1995
更新日期:1995-12-01 00:00:00
abstract::The herpesvirus tegument is a layer of viral and cellular proteins located between the capsid and envelope of the virion. The VP1/2 tegument protein is critical for the propagation of all herpesviruses examined. Using an infectious clone of the alphaherpesvirus pseudorabies virus, we have made a collection of truncati...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01184-06
更新日期:2006-12-01 00:00:00
abstract::The infection of human fetal thymus organ cultures (FTOC) with coxsackievirus B4 E2 (CVB4 E2) was investigated. Both positive- and negative-strand viral RNA were detected by real-time quantitative reverse transcription-PCR (RT-PCR) in CVB4 E2-infected FTOC, which supported high yields of virus production (approximatel...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.18.9854-9861.2004
更新日期:2004-09-01 00:00:00
abstract::Mlvi-1 defines a locus of proviral integration in rat thymomas induced by Moloney murine leukemia virus. pvt-1/Mis-1 represents an independently identified locus which becomes rearranged either by chromosomal translocation in murine plasmacytomas or by provirus insertion in retrovirus-induced murine and rat thymic lym...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.5.2366-2369.1989
更新日期:1989-05-01 00:00:00
abstract::The Epstein-Barr Virus (EBV) immediate-early protein BRLF1 is one of two transactivators which mediate the switch from latent to lytic replication in EBV-infected cells. DNA viruses often modulate the function of critical cell cycle proteins to maximize the efficiency of virus replication. Here we have examined the ef...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.8.6540-6550.1999
更新日期:1999-08-01 00:00:00
abstract::Japanese encephalitis virus (JEV) is a mosquito-borne flavivirus and one of the most common agents of viral encephalitis. The infectious entry process of JEV into host cells remains largely unknown. Here, we present a systemic study concerning the cellular entry mechanism of JEV to B104 rat neuroblastoma cells. It was...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00903-12
更新日期:2012-12-01 00:00:00