Abstract:
:We have previously described a nonconditional mutant of avian sarcoma virus (SE21Q1b) which fails to package viral RNA (Gallis et al., Virology 94:146-161, 1979; Linial et al., Cell 15:1371-1381, 1978). Quail cells transformed by SE21Q1b contain normal amounts of intracellular viral mRNA's for src, env, and gag-pol and release particles with the density of normal virus containing a typical complement of virion proteins, including reverse transcriptase. These virions are noninfectious for both chicken and quail cells and contain primarily cellular rather than viral RNA. Analysis by gel electrophoresis of the cellular DNA of quail cells transformed by SE21Q1b after restriction endonuclease digestion indicated the presence of a single provirus. The provirus was located at one site in the genome of the host cell and was flanked by the characteristic terminally repeated sequences derived from the 3' and 5' ends of viral RNA. The only defect detected in the SE21Q1b provirus was a deletion of ca. 150 base pairs of DNA somewhere between 300 and 600 bases from the left (gag-pol) end of the provirus. Analyses of the proviral DNA of cells transformed by wild-type recombinants between SE21Q1b and leukosis viruses reveal that the recombinants no longer contain this deletion. The deletion, therefore, defines a region on the viral RNA which is required for correct packaging of the virion RNA.
journal_name
J Viroljournal_title
Journal of virologyauthors
Shank PR,Linial Mdoi
10.1128/JVI.36.2.450-456.1980subject
Has Abstractpub_date
1980-11-01 00:00:00pages
450-6issue
2eissn
0022-538Xissn
1098-5514journal_volume
36pub_type
杂志文章abstract::Human immunodeficiency virus type 1 infection of mononuclear phagocytes has been implicated in disease manifestations, but postentry viral replication events in these cells have not been well characterized. Productive infection of activated T cells is associated with cell proliferation and accumulation of full-length ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.2.1258-1263.1994
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abstract::The degree of genetic variability in the hypervariable region 1 of hepatitis C virus (HCV) was analyzed by cloning and sequencing HCV genomes obtained in paired samples of serum, liver tissue, and peripheral blood mononuclear cells (PBMC) from four chronic hepatitis C patients. Genetic variability in serum was higher ...
journal_title:Journal of virology
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doi:10.1128/JVI.72.2.1640-1646.1998
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pub_type: 杂志文章
doi:10.1128/JVI.2.3.178-181.1968
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.2.1630-1636.1999
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.4.2425-2432.1994
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01088-17
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doi:10.1128/JVI.02470-06
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pub_type: 杂志文章
doi:10.1128/jvi.76.16.8468-8471.2002
更新日期:2002-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.75.14.6710-6713.2001
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.5.1134-1142.1974
更新日期:1974-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.03427-14
更新日期:2015-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02687-14
更新日期:2015-01-15 00:00:00
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pub_type: 杂志文章
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更新日期:2007-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.12.7622-7629.1995
更新日期:1995-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.1.746-749.1997
更新日期:1997-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.5.1746-1752.1988
更新日期:1988-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2000-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.80.7.3523-3531.2006
更新日期:2006-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.12.8102-8108.1995
更新日期:1995-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.9.5278-5286.1995
更新日期:1995-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.15.4.812-819.1975
更新日期:1975-04-01 00:00:00
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更新日期:2012-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01903-18
更新日期:2019-02-05 00:00:00
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journal_title:Journal of virology
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更新日期:2015-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2017-04-28 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2007-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.7.4283-4290.1996
更新日期:1996-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.6.2895-2900.1990
更新日期:1990-06-01 00:00:00