Abstract:
:Viruses display a wide range of genomic profiles and, consequently, a variety of gene expression strategies. Specific sequences associated with transcriptional processes have been described in viruses, and putative promoter motifs have been elucidated for some nucleocytoplasmic large DNA viruses (NCLDV). Among NCLDV, the Marseilleviridae is a well-recognized family because of its genomic mosaicism. The marseilleviruses have an ability to incorporate foreign genes, especially from sympatric organisms inhabiting Acanthamoeba, its main known host. Here, we identified for the first time an eight-nucleotide A/T-rich promoter sequence (AAATATTT) associated with 55% of marseillevirus genes that is conserved in all marseilleviruses lineages, a higher level of conservation than that of any giant virus described to date. We instigated our prediction about the promoter motif by biological assays and by evaluating how single mutations in this octamer can impact gene expression. The investigation of sequences that regulate the expression of genes relative to lateral transfer revealed that the promoter motifs do not appear to be incorporated by marseilleviruses from donor organisms. Indeed, analyses of the intergenic regions that regulate lateral gene transfer-related genes have revealed an independent origin of the marseillevirus intergenic regions that does not match gene-donor organisms. About 50% of AAATATTT motifs spread throughout intergenic regions of the marseilleviruses are present as multiple copies. We believe that such multiple motifs are associated with increased expression of a given gene or are related to incorporation of foreign genes into the mosaic genome of marseilleviruses.IMPORTANCE The marseilleviruses draw attention because of the peculiar features of their genomes; however, little is known about their gene expression patterns or the factors that regulate those expression patterns. The limited published research on the expression patterns of the marseilleviruses and their unique genomes has led us to study the promoter motif sequences in the intergenic regions of the marseilleviruses. This work is the first to analyze promoter sequences in the genomes of the marseilleviruses. We also suggest a strong capacity to acquire foreign genes and to express those genes mediated by multiple copies of the promoter motifs available in intergenic regions. These findings contribute to an understanding of genomic expansion and plasticity observed in these giant viruses.
journal_name
J Viroljournal_title
Journal of virologyauthors
Oliveira GP,Lima MT,Arantes TS,Assis FL,Rodrigues RAL,da Fonseca FG,Bonjardim CA,Kroon EG,Colson P,La Scola B,Abrahão JSdoi
10.1128/JVI.01088-17subject
Has Abstractpub_date
2017-10-13 00:00:00issue
21eissn
0022-538Xissn
1098-5514pii
JVI.01088-17journal_volume
91pub_type
杂志文章abstract::The majority of T-cell clones derived from a donor who experienced dengue illness following receipt of a live experimental dengue virus type 3 (DEN3) vaccine cross-reacted with all four serotypes of dengue virus, but some were serotype specific or only partially cross-reactive. The nonstructural protein, NS3, was immu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.5.3108-3117.1996
更新日期:1996-05-01 00:00:00
abstract::The human T-cell leukemia virus (HTLV) Rex protein is essential for efficient expression of the viral structural and enzymatic gene products. In this study, we assessed the role of the HTLV-2 rex gene in viral RNA expression and Gag protein production. Following transfection of human JM4 T cells with wild-type and rex...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.10.8112-8119.1999
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abstract::Serological surveys have revealed that 30 to 50% of wild-caught African green monkeys have antibodies reactive to simian immunodeficiency virus (SIV), a retrovirus related to human immunodeficiency virus (HIV). Although the nucleotide sequence of one SIVagm isolate, Tyo1, was recently reported, the extent of genetic v...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.4.1800-1802.1989
更新日期:1989-04-01 00:00:00
abstract::Host cell-mediated proteolytic cleavage of the human immunodeficiency virus type 1 (HIV-1) gp160 precursor glycoprotein into gp120 and gp41 subunits is required to generate fusion-competent envelope glycoprotein (Env) spikes. The gp120-directed broadly neutralizing monoclonal antibodies (bNabs) isolated from HIV-infec...
journal_title:Journal of virology
pub_type: 杂志文章
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abstract::Influenza A viruses (IAVs) remain a significant public health threat, causing more than 300,000 hospitalizations in the United States during the 2015-2016 season alone. While only a few IAVs of avian origin have been associated with human infections, the ability of these viruses to cause zoonotic infections further in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02011-18
更新日期:2019-03-05 00:00:00
abstract:UNLABELLED:Intron-containing mRNAs are subject to restricted nuclear export in higher eukaryotes. Retroviral replication requires the nucleocytoplasmic transport of both spliced and unspliced RNA transcripts, and RNA export mechanisms of gammaretroviruses are poorly characterized. Here, we report the involvement of the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03584-13
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01623-08
更新日期:2008-12-01 00:00:00
abstract::Infectious hematopoietic necrosis virus (IHNV) is an important fish pathogen that infects both wild and cultured salmonids. As a species of the genus Novirhabdovirus, IHNV is a valuable model system for exploring the host entry mechanisms of rhabdoviruses. In this study, quantum dots (QDs) were used as fluorescent lab...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00109-11
更新日期:2011-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.1.251-258.2002
更新日期:2002-01-01 00:00:00
abstract::Herpes simplex virus type 1 (HSV-1) infection causes the active degradation of the catalytic subunit of DNA-dependent protein kinase (DNA-PKcs), and this process is reliant on the expression of the HSV-1 immediate-early protein Vmw110. In this study we investigated in more detail the mechanism by which the degradation...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.1.650-657.1999
更新日期:1999-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.1.379-389.1994
更新日期:1994-01-01 00:00:00
abstract::Three fish retroviruses infecting walleyes constitute the recently recognized genus called epsilonretrovirus. The founding member of this group, walleye dermal sarcoma virus (WDSV), induces benign skin tumors in the infected fish and replicates near 4 degrees C. While the viral genomic sequence is known, biochemical c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.9.4341-4349.2002
更新日期:2002-05-01 00:00:00
abstract:UNLABELLED:PB1-F2 protein, the 11th influenza A virus (IAV) protein, is considered to play an important role in primary influenza virus infection and postinfluenza secondary bacterial pneumonia in mice. The functional role of PB1-F2 has been reported to be a strain-specific and host-specific phenomenon. Its precise con...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01551-15
更新日期:2015-10-14 00:00:00
abstract::Influenza A viruses (IAVs) are viral pathogens that cause epidemics and occasional pandemics of significant mortality. The generation of efficacious vaccines and antiviral drugs remains a challenge due to the rapid appearance of new influenza virus types and antigenic variants. Consequently, novel strategies for the p...
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pub_type: 杂志文章
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更新日期:2019-02-05 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.18.9481-9492.2002
更新日期:2002-09-01 00:00:00
abstract::Two forms of herpes simplex virus glycoprotein gD were recombined into Autographa californica nuclear polyhedrosis virus (baculovirus) and expressed in infected Spodoptera frugiperda (Sf9) cells. Each protein was truncated at residue 306 of mature gD. One form, gD-1(306t), contains the coding sequence of Patton strain...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.2.766-775.1994
更新日期:1994-02-01 00:00:00
abstract::The present study used the cottontail rabbit papillomavirus DNA-rabbit system to evaluate whether the regulatory genes E1 and E2 and the transforming gene E6 are required for papilloma formation. Frameshift mutations were generated in the individual genes in the context of a full-length cottontail rabbit papillomaviru...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.9.6097-6102.1994
更新日期:1994-09-01 00:00:00
abstract::African swine fever virus (ASFV) is a complex, cytoplasmic double-stranded DNA (dsDNA) virus that is currently expanding throughout the world. Currently, circulating virulent genotype II Armenia/07-like viruses cause fatal disease in pigs and wild boar, whereas attenuated strains induce infections with various levels ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02298-18
更新日期:2019-05-29 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.3.1967-1973.1998
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.10.5513-5523.1991
更新日期:1991-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.3.1352-1366.2004
更新日期:2004-02-01 00:00:00
abstract:UNLABELLED:Foreskin is the principal site of heterosexual HIV-1 infection in men. However, little is known about HIV-1-specific immune responses or inflammation in foreskin. To the best of our knowledge, no previous studies have assessed immune responses to candidate HIV-1 vaccines in foreskin. Using the rhesus monkey ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03771-13
更新日期:2014-04-01 00:00:00
abstract::Galectin-9 is a pleiotropic immune modulator affecting numerous cell types of innate and adaptive immunity. Patients with chronic infection with either hepatitis C virus (HCV) or HIV have elevated circulating levels. Limited data exist on the regulation of natural killer (NK) cell function through interaction with gal...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01085-12
更新日期:2013-05-01 00:00:00
abstract::Antibody was raised against purified vaccinia virus RNA polymerase and used to screen a recombinant vaccinia virus-lambda gt11 library. The DNA from several immunopositive clones was shown by Southern hybridization to originate from the vaccinia virus HindIII E fragment. The nucleotide sequence of the RNA polymerase s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.11.5376-5382.1990
更新日期:1990-11-01 00:00:00
abstract::Kaposi's sarcoma-associated herpesvirus (KSHV) latently infects KS tumors, primary effusion lymphomas (PELs), and PEL cell lines. K12 (T0.7) is the most abundant transcript expressed in latent KSHV infection. We characterize here the K12 transcript from a PEL tumor prior to passage in cell culture. The PEL tumor KSHV ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.23.11880-11888.2002
更新日期:2002-12-01 00:00:00
abstract::Some of the properties of a new human papovavirus, BK, have been examined. Host range studies of BK virus (BKV) showed human cells to be more sensitive to infection than monkey cells; human fetal brain cells appear to be highly sensitive to BKV, with the production of extensive cytopathology characterized by cytoplasm...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.12.3.625-631.1973
更新日期:1973-09-01 00:00:00
abstract::The mature virion of the alphaherpesvirus pseudorabies virus (PrV) contains a minimum of 31 structural proteins which are recruited into the virus particle by a network of protein-protein interactions which is only incompletely understood. We show here that deletion of the tegument protein pUL21 resulted in a drastic ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01801-06
更新日期:2007-01-01 00:00:00
abstract::Transfection of the E6 and E7 genes of the high-risk human papillomaviruses (HPVs) into primary genital keratinocytes generates colonies of proliferating cells which are resistant to calcium- and serum-induced terminal differentiation. To genetically map the HPV gene(s) responsible for this cellular phenotype, we clon...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.5.3269-3279.1996
更新日期:1996-05-01 00:00:00
abstract::The functions of bovine respiratory syncytial virus (BRSV) nonstructural proteins NS1 and NS2 were studied by generation and analysis of recombinant BRSV carrying single and double gene deletions. Whereas in MDBK cells the lack of either or both NS genes resulted in a 5,000- to 10,000-fold reduction of virus titers, i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.18.8234-8242.2000
更新日期:2000-09-01 00:00:00
abstract::Circulating leukocytes are important in dissemination of cytomegalovirus (CMV) infection in humans. In the mouse model of murine CMV infection (MCMV), it has been shown that infection peaks on days 5 to 7 after experimental infection, when 0.01 to 0.1% of the circulating leukocytes contain viral DNA. In our laboratory...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.10.6305-6311.1994
更新日期:1994-10-01 00:00:00