Abstract:
:Influenza A viruses (IAVs) remain a significant public health threat, causing more than 300,000 hospitalizations in the United States during the 2015-2016 season alone. While only a few IAVs of avian origin have been associated with human infections, the ability of these viruses to cause zoonotic infections further increases the public health risk of influenza. Of these, H9N2 viruses in Asia are of particular importance as they have contributed internal gene segments to other emerging zoonotic IAVs. Notably, recent H9N2 viruses have acquired molecular markers that allow for a transition from avian-like to human-like terminal sialic acid (SA) receptor recognition via a single amino acid change at position 226 (H3 numbering), from glutamine (Q226) to leucine (L226), within the hemagglutinin (HA) receptor-binding site (RBS). We sought to determine the plasticity of amino acid 226 and the biological effects of alternative amino acids on variant viruses. We created a library of viruses with the potential of having any of the 20 amino acids at position 226 on a prototypic H9 HA subtype IAV. We isolated H9 viruses that carried naturally occurring amino acids, variants found in other subtypes, and variants not found in any subtype at position 226. Fitness studies in quails revealed that some natural amino acids conferred an in vivo replication advantage. This study shows the flexibility of position 226 of the HA of H9 influenza viruses and the resulting effect of single amino acid changes on the phenotype of variants in vivo and in vitroIMPORTANCE A single amino acid change at position 226 in the hemagglutinin (HA) from glutamine (Q) to leucine (L) has been shown to play a key role in receptor specificity switching in various influenza virus HA subtypes, including H9. We tested the flexibility of amino acid usage and determined the effects of such changes. The results reveal that amino acids other than L226 and Q226 are well tolerated and that some amino acids allow for the recognition of both avian and human influenza virus receptors in the absence of other changes. Our results can inform better avian influenza virus surveillance efforts as well as contribute to rational vaccine design and improve structural molecular dynamics algorithms.
journal_name
J Viroljournal_title
Journal of virologyauthors
Obadan AO,Santos J,Ferreri L,Thompson AJ,Carnaccini S,Geiger G,Gonzalez Reiche AS,Rajão DS,Paulson JC,Perez DRdoi
10.1128/JVI.02011-18subject
Has Abstractpub_date
2019-03-05 00:00:00issue
6eissn
0022-538Xissn
1098-5514pii
JVI.02011-18journal_volume
93pub_type
杂志文章abstract::Host cell interactions of human adenovirus serotypes belonging to subgroups B (adenovirus type 3 [Ad3] and Ad7) and C (Ad2 and Ad5) were comparatively analyzed at three levels: (i) binding of virus particles with host cell receptors; (ii) cointernalization of macromolecules with adenovirions; and (iii) adenovirus-indu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.8.3661-3673.1990
更新日期:1990-08-01 00:00:00
abstract::An immediate-early protein of murine cytomegalovirus (MCMV), pp89, elicits an immunodominant and protective major histocompatibility complex (MHC) class I Ld-restricted CD8+ T-lymphocyte response. Remarkably, presentation of the naturally processed peptide of pp89, the nonapeptide YPHFMPTNL, is abolished during permis...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.1.289-297.1994
更新日期:1994-01-01 00:00:00
abstract::Chimeric bovine respiratory syncytial viruses (BRSV) expressing glycoproteins of bovine parainfluenza virus type 3 (BPIV-3) instead of BRSV glycoproteins were generated from cDNA. In the BRSV antigenome cDNA, the open reading frames of the major BRSV glycoproteins, attachment protein G and fusion protein F, were repla...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.19.9367-9377.2001
更新日期:2001-10-01 00:00:00
abstract::A gene encoding the large T antigen of polyomavirus was inserted into the baculovirus Autographa californica nuclear polyhedrosis virus so that gene expression was under the control of the strong, very late polyhedrin gene promoter. Significantly more large T antigen was produced in recombinant virus-infected insect c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.5.1712-1716.1987
更新日期:1987-05-01 00:00:00
abstract::The assembly of hepatitis C virus (HCV) is poorly understood, largely due to the lack of mammalian cell culture systems that are easily manipulated and produce high titers of virus. This problem is highlighted by the inability of the recently established HCV replicon systems to support HCV capsid assembly despite high...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.17.9257-9269.2004
更新日期:2004-09-01 00:00:00
abstract::In order to clarify the effect of an accumulation of amino acid substitutions on the hemadsorption character of the influenza AH3 virus hemagglutinin (HA) protein, we introduced single-point amino acid changes into the HA1 domain of the HA proteins of influenza viruses isolated in 1968 (A/Aichi/2/68) and 1997 (A/Sydne...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.10.6472-6477.2005
更新日期:2005-05-01 00:00:00
abstract:UNLABELLED:An altered intestinal microbiome during chronic human immunodeficiency virus (HIV) infection is associated with mucosal dysfunction, inflammation, and disease progression. We performed a preclinical evaluation of the safety and efficacy of fecal microbiota transplantation (FMT) as a potential therapeutic in ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00099-16
更新日期:2016-04-29 00:00:00
abstract::We previously reported the establishment of several lymphoid cell lines from X-ray-induced thymomas of C57BL/Ka mice, and all, except one, produce retroviruses (P. Sankar-Mistry and P. Jolicoeur, J. Virol.35:270-275, 1980). Biological characterization of five of these new primary radiation leukemia viruses (RadLVs) in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.45.2.565-575.1983
更新日期:1983-02-01 00:00:00
abstract::By preannealing a radioactive, representative Moloney murine leukemia virus (M-MuLV) cDNA with large excesses of AKR 70S viral RNA, an M-MuLV-specific cDNA has been prepared. When hybridized to restriction enzyme fragments of M-MuLV-infected mouse cell DNA, the preannealed probe recognizes integrated M-MuLV DNA and do...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.33.3.1074-1082.1980
更新日期:1980-03-01 00:00:00
abstract:UNLABELLED:Since the 1960s, simian hemorrhagic fever virus (SHFV; Nidovirales, Arteriviridae) has caused highly fatal outbreaks of viral hemorrhagic fever in captive Asian macaque colonies. However, the source(s) of these outbreaks and the natural reservoir(s) of this virus remain obscure. Here we report the identifica...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02203-14
更新日期:2014-11-01 00:00:00
abstract::The foot-and-mouth disease virus (FMDV) capsid protein precursor, P1-2A, is cleaved by 3C(pro) to generate VP0, VP3, VP1, and the peptide 2A. The capsid proteins self-assemble into empty capsid particles or viruses which do not contain 2A. In a cell culture-adapted strain of FMDV (O1 Manisa [Lindholm]), three differen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01863-13
更新日期:2013-11-01 00:00:00
abstract::Viruses are considered to use vesicular trafficking in infected cells, but the details of assembly/release pathways of hepatitis B virus (HBV) are still unknown. To identify key regulators of HBV production, we performed short interfering RNA (siRNA) screening for Rab proteins, which are considered to act as molecular...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00621-19
更新日期:2019-07-17 00:00:00
abstract::Recent studies have demonstrated that influenza A virus infection activates the phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway by binding of influenza NS1 protein to the p85 regulatory subunit of PI3K. Our previous study proposed that two polyproline motifs in NS1 (amino acids 164 to 167 [PXXP], SH3 bindin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01427-07
更新日期:2007-12-01 00:00:00
abstract::Airborne viruses are expected to be ubiquitous in the atmosphere but they still remain poorly understood. This study investigated the temporal and spatial dynamics of airborne viruses and their genotypic characteristics in air samples collected from three distinct land use types (a residential district [RD], a forest ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00293-12
更新日期:2012-08-01 00:00:00
abstract::It is not yet clear to what extent depletion of intracellular GTP pools contributes to the antiviral activity of ribavirin. Therefore, the antiviral activities of (i) ribavirin, (ii) its 5-ethynyl analogue, 5-ethynyl-1-beta-D-ribofuranosylimidazole-4-carboxamide (EICAR), and (iii) mycophenolic acid (MPA) (a compound t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.3.1943-1947.2005
更新日期:2005-02-01 00:00:00
abstract::Baculoviruses, although they infect insects in nature, can transduce a wide variety of mammalian cells and are therefore promising gene therapy vectors. However, baculovirus transduction into many mammalian cells is very inefficient, and the limiting stages and factors remain unknown. An important finding is that a sh...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00709-19
更新日期:2019-07-17 00:00:00
abstract::Sequence analysis of the Lymantria dispar multicapsid nucleopolyhedrovirus (LdMNPV) genome identified an open reading frame (ORF) encoding a 548-amino-acid (62-kDa) protein that showed 35% amino acid sequence identity with vaccinia virus ATP-dependent DNA ligase. Ligase homologs have not been reported from other bacul...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.11.9142-9149.1998
更新日期:1998-11-01 00:00:00
abstract::There is controversy as to whether the cell entry mechanism of Sindbis virus (SIN) involves direct fusion of the viral envelope with the plasma membrane at neutral pH or uptake by receptor-mediated endocytosis and subsequent low-pH-induced fusion from within acidic endosomes. Here, we studied the membrane fusion activ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.10.8476-8484.1999
更新日期:1999-10-01 00:00:00
abstract::Recombinant Epstein-Barr virus (EBV) with a selectable marker successfully infected the human gastric carcinoma cell lines AGS, MKN28, and MKN74. Following incubation in selective media, drug-resistant cell clones were isolated and proved to be infected with EBV. All gastric carcinoma cell clones were positive for EBN...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.7.5688-5691.1997
更新日期:1997-07-01 00:00:00
abstract::Herpes simplex virus virions contain one or more functions which mediate shutoff of host protein synthesis, disaggregation of host polyribosomes, and degradation of host mRNA. We studied aspects of the host shutoff mechanism by using herpes simplex virus type 1 mutants deficient in virion-induced shutoff of host prote...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.7.2198-2207.1987
更新日期:1987-07-01 00:00:00
abstract::BALB/c mice treated with pristane and Abelson virus have been used as an animal model system for the rapid induction of plasmacytomas. Myelomonocytic tumors with helper Moloney murine leukemia virus clonally inserted into the c-myb locus were observed in about 10% of pristane-primed BALB/c mice infected with Abelson v...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.12.3721-3725.1987
更新日期:1987-12-01 00:00:00
abstract::The rat-derived Harvey murine sarcoma virus (Ha-MuSV) contains a transduced ras oncogene activated by two missense mutations and flanked by rat retroviruslike VL30 sequences. Ha-MuSV induces focal transformation of mouse NIH 3T3 cells in vitro and tumors (fibrosarcomas and splenic erythroleukemias) in newborn mice. We...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.3.1384-1392.1989
更新日期:1989-03-01 00:00:00
abstract::Adeno-associated virus (AAV) integrates very efficiently into a specific site (AAVS1) of human chromosome 19. Two elements of the AAV genome are sufficient: the inverted terminal repeats (ITRs) and the Rep78 or Rep68 protein. The incorporation of the AAV integration machinery in nonviral delivery systems is of great i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.9.7653-7658.1998
更新日期:1998-09-01 00:00:00
abstract::Nonpermissive 3T3 cells were infected with purified superhelical simian virus 40 (SV40) deoxyribonucleic acid I (DNA I). One hour after infection, approximately 60% of the intracellular SV40 DNA was converted to relaxed forms. One day after infection, all intracellular SV40 DNA was present as slow-sedimenting material...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.10.3.425-432.1972
更新日期:1972-09-01 00:00:00
abstract::Retrovirus assembly involves a complex series of events in which a large number of proteins must be targeted to a point on the plasma membrane where immature viruses bud from the cell. Gag polyproteins of most retroviruses assemble an immature capsid on the cytoplasmic side of the plasma membrane during the budding pr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.2.784-795.2000
更新日期:2000-01-01 00:00:00
abstract::Retroviral Gag proteins direct the assembly and release of virus particles from the plasma membrane. The budding machinery consists of three small domains, the M (membrane-binding), I (interaction), and L (late or "pinching-off") domains. In addition, Gag proteins contain sequences that control particle size. For Rous...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.14.6527-6536.2001
更新日期:2001-07-01 00:00:00
abstract::Adenovirus deletion mutants were studied to examine the influence of the 5' untranslated sequence on the translation of early region 1A mRNAs. Alterations of the 5' untranslated sequence, including complete deletion of the wild-type 5' untranslated sequence, did not significantly affect the rate of translation. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.51.3.884-888.1984
更新日期:1984-09-01 00:00:00
abstract::Our study was designed to investigate the nature of an antigen-specific suppressor factor generated by antigen-stimulated herpes simplex virus (HSV)-immune splenocytes. Factor SF-200, a 90,000- to 100,000-dalton fraction obtained after Sephacryl gel filtration, suppressed the generation of HSV-specific cytotoxic T-lym...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.58.2.331-338.1986
更新日期:1986-05-01 00:00:00
abstract::A temperature-sensitive mutant of adenovirus 31 was defective in formation of the DNA replication complex, suggesting the existence of a virus-coded protein necessary for the complex-formation. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.2.538-540.1974
更新日期:1974-02-01 00:00:00
abstract::The cellular site of herpesvirus tegument assembly has yet to be defined. We have previously used a recombinant herpes simplex virus type 1 expressing a green fluorescent protein (GFP)-tagged tegument protein, namely VP22, to show that VP22 is localized exclusively to the cytoplasm during infection. Here we have const...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.6.2575-2583.2001
更新日期:2001-03-01 00:00:00