Abstract:
:We recently demonstrated that members of the c-ets proto-oncogene family, Ets1 and Ets2, are sequence-specific transcriptional activators of the human T-lymphotropic virus type I (HTLV-I) long terminal repeat (LTR). We now report that the HTLV-I LTR contains two distinct Ets1-responsive regions, ERR-1 and ERR-2. Expression of Ets1 with reporter plasmids containing ERR-1 or ERR-2 upstream of a basal promoter resulted in an increase in transcriptional activity. By gel mobility shift assay, the interaction of Ets1 with the downstream ERR-1-binding region was found to be more stable than its interaction with the upstream ERR-2 region. By DNase I footprint, gel mobility shift, and methylation interference analyses, ERR-1 was found to contain two Ets1 binding sites, ERE-A and ERE-B. A recombinant Ets1 protein was found to bind with higher affinity to ERE-A than to ERE-B. Binding of Ets1 to these sites appears to result in a specific and sequential protection of a 37-nucleotide sequence of the HTLV-I LTR from -154 to -118. In view of the high-level expression of Ets1 in lymphoid cells, the c-ets proto-oncogenes encode transcription factors which could play an important role in both basal and Tax1-mediated HTLV-I transcription.
journal_name
J Viroljournal_title
Journal of virologyauthors
Gitlin SD,Bosselut R,Gégonne A,Ghysdael J,Brady JNdoi
10.1128/JVI.65.10.5513-5523.1991subject
Has Abstractpub_date
1991-10-01 00:00:00pages
5513-23issue
10eissn
0022-538Xissn
1098-5514journal_volume
65pub_type
杂志文章abstract::The degree of genetic variability in the hypervariable region 1 of hepatitis C virus (HCV) was analyzed by cloning and sequencing HCV genomes obtained in paired samples of serum, liver tissue, and peripheral blood mononuclear cells (PBMC) from four chronic hepatitis C patients. Genetic variability in serum was higher ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.2.1640-1646.1998
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doi:10.1128/JVI.33.1.438-448.1980
更新日期:1980-01-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/JVI.00158-12
更新日期:2012-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.69.1.357-364.1995
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.26.1.136-142.1978
更新日期:1978-04-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/JVI.01926-20
更新日期:2021-01-28 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.9.5827-5831.1996
更新日期:1996-09-01 00:00:00
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pub_type: 杂志文章,评审
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.12.7181-7189.1993
更新日期:1993-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:1983-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.13.5988-5996.2000
更新日期:2000-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.5.2922-2927.1993
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.2.1181-1189.1995
更新日期:1995-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.7.4117-4125.1994
更新日期:1994-07-01 00:00:00