Abstract:
:The 86-kDa IE2 protein (IE86) of human cytomegalovirus (HCMV) can act as both an activator and a repressor of gene expression. The mechanisms for both of these functions are not well defined. It has recently been demonstrated that this protein has sequence-specific DNA binding properties: it interacts directly with a target sequence that is located between the TATA box and the cap site of its own promoter. This sequence, termed the CRS (cis repression signal) element, is required for negative autoregulation of the IE1/IE2 enhancer/promoter by IE2. We demonstrate now that binding of this protein to DNA is not confined to this site but occurs also within an early promoter of HCMV that has previously been shown to be strongly IE2 responsive. By DNase I protection analysis using a purified, procaryotically expressed IE2 protein, we could identify three binding sites within the region of -290 to -120 of the UL112 promoter of HCMV. Competition in DNase I protection experiments as well as gel retardation experiments showed that the identified binding sites are specific and have high affinity. Deletion of IE2 binding sites from this promoter reduced the level of transactivation; however, the remaining promoter could still be stimulated about 40-fold. Constructs in which IE2 binding sites were fused directly to the TATA box of the UL112 promoter did not reveal a significant contribution of these sequences to transactivation. However, if an IE2 binding site was reinserted upstream of nucleotide -117 of the UL112 promoter, an increase in transactivation by IE2 was obvious, whereas a mutated sequence could not mediate this effect. This finding suggests that DNA-bound IE2 can contribute to transactivation but seems to require the presence of additional transcription factors. Moreover, a comparison of the detected IE2 binding sites could not detect a strong homology, suggesting that this protein may be able to interact with a broad spectrum of different target sequences.
journal_name
J Viroljournal_title
Journal of virologyauthors
Arlt H,Lang D,Gebert S,Stamminger Tdoi
10.1128/JVI.68.7.4117-4125.1994subject
Has Abstractpub_date
1994-07-01 00:00:00pages
4117-25issue
7eissn
0022-538Xissn
1098-5514journal_volume
68pub_type
杂志文章abstract::Adult T-cell leukemia (ATL) occurs in a small population of human T-cell leukemia virus type 1 (HTLV-1)-infected individuals. Although the critical risk factor for ATL development is not clear, it has been noted that ATL is incidentally associated with mother-to-child infection, elevated proviral loads, and weakness i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.5.2956-2963.2003
更新日期:2003-03-01 00:00:00
abstract:UNLABELLED:Varicella-zoster virus (VZV) is an alphaherpesvirus that causes varicella and herpes zoster. Membrane fusion is essential for VZV entry and the distinctive syncytium formation in VZV-infected skin and neuronal tissue. Herpesvirus fusion is mediated by a complex of glycoproteins gB and gH-gL, which are necess...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00792-16
更新日期:2016-07-27 00:00:00
abstract:UNLABELLED:Semen enhances HIV infection in vitro, but how long it retains this activity has not been carefully examined. Immediately postejaculation, semen exists as a semisolid coagulum, which then converts to a more liquid form in a process termed liquefaction. We demonstrate that early during liquefaction, semen exh...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00269-14
更新日期:2014-07-01 00:00:00
abstract::Measles virus (MeV) infection is characterized by the formation of multinuclear giant cells (MGC). We report that beta interferon (IFN-beta) production is amplified in vitro by the formation of virus-induced MGC derived from human epithelial cells or mature conventional dendritic cells. Both fusion and IFN-beta respon...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00078-07
更新日期:2007-12-01 00:00:00
abstract::The three primary capsid proteins (A, B, and C) of adeno-associated viruses have been shown previously to contain overlapping amino acid sequences (R. McPherson and J. Rose, J. Virol. 46:523-529, 1983). In the present study we demonstrate definitively that these proteins are encoded in the right half of the adeno-asso...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.52.2.591-597.1984
更新日期:1984-11-01 00:00:00
abstract:UNLABELLED:Influenza virus hemagglutinin (HA) mediates virus entry by binding to cell surface receptors and fusing the viral and endosomal membranes following uptake by endocytosis. The acidic environment of endosomes triggers a large-scale conformational change in the transmembrane subunit of HA (HA2) involving a loop...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00939-15
更新日期:2015-10-01 00:00:00
abstract::Examination of the interaction between human immunodeficiency virus (HIV) regulatory gene products and the host immune system is fundamental to understanding the pathogenesis of HIV and could reveal possible targets for therapeutic intervention in the treatment of AIDS. The HIV Tat gene is a potential candidate for th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.12.7622-7629.1995
更新日期:1995-12-01 00:00:00
abstract::RNA plant viruses use various translational regulatory mechanisms to control their gene expression. Translational enhancement of viral mRNAs that leads to higher levels of protein synthesis from specific genes may be essential for the virus to successfully compete for cellular translational machinery. The control elem...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.3.1144-1153.2002
更新日期:2002-02-01 00:00:00
abstract::Recombinant interleukin-16 (rIL-16) has been found to inhibit human immunodeficiency virus type 1 (HIV-1) replication in acutely or endogenously infected CD4(+) T cells. However, the effect of rIL-16 on HIV-1 replication in antigen-presenting cells (APCs) is still unknown. We show here a potent HIV-suppressive activit...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.8.7008-7013.1999
更新日期:1999-08-01 00:00:00
abstract::The infection of cells which belong to the B-cell lineage is thought to be the primary event leading to the phenotypic and functional alterations seen in the murine AIDS (M. Huang, C. Simard, D. Kay, and P. Jolicoeur, J. Virol. 65:6562-6571, 1991). Using in situ hybridization, we studied the time course of the anatomi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.3.1903-1912.1994
更新日期:1994-03-01 00:00:00
abstract::The major structural glycoprotein E2 of classical swine fever virus (CSFV) is responsible for eliciting neutralizing antibodies and conferring protective immunity. The current structural model of this protein predicts its surface-exposed region at the N terminus with a short stretch of the C-terminal residues spanning...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.24.11619-11625.2000
更新日期:2000-12-01 00:00:00
abstract::Recombinant DNA clones of a viral clone of spleen necrosis virus, an avian retrovirus, were found to have long terminal repeats of different sizes. The variation was in the U3 region of the long terminal repeats, and any one clone had U3 of the same size in both long terminal repeats. The U3 regions in the 5' and 3' l...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.41.1.163-171.1982
更新日期:1982-01-01 00:00:00
abstract:UNLABELLED:Major histocompatibility complex class II (MHC-II) molecules play a central role in adaptive antiviral immunity by presenting viral peptides to CD4(+) T cells. Due to their key role in adaptive immunity, many viruses, including Kaposi's sarcoma-associated herpesvirus (KSHV), have evolved multiple strategies ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03713-14
更新日期:2015-05-01 00:00:00
abstract::In two cases of parenteral transmission of human immunodeficiency virus type 1 (HIV-1) syncitium-inducing (SI) variants, we previously observed selection for macrophagetropic variants. Although infection of macrophages is generally mediated via CCR5, we found no selection for SI variants that could use CCR5 as corecep...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.75.18.8848-8853.2001
更新日期:2001-09-01 00:00:00
abstract::Integrin-using rotaviruses bind MA104 cell surface alpha2beta1 integrin via the Asp-Gly-Glu (DGE) sequence in virus spike protein VP4 and interact with alphaxbeta2 integrin during cell entry through outer capsid protein VP7. Infection is inhibited by the alpha2beta1 ligand Asp-Gly-Glu-Ala (DGEA) and the alphaxbeta2 li...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.21.11786-11797.2004
更新日期:2004-11-01 00:00:00
abstract::This paper is concerned with the physiological role(s) of T4 phage-coded dihydrofolate reductase, which functions both in DNA precursor metabolism and as a virion protein. (i) We have detected enzyme activity in noninfectious particles produced under restrictive conditions by gene 11 mutants. This supports the conclus...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.23.3.645-658.1977
更新日期:1977-09-01 00:00:00
abstract::Epstein-Barr virus (EBV) is a herpesvirus commonly associated with several malignancies, particularly in immunocompromised hosts. As a strategy for stimulating immunity against EBV for the treatment of EBV-associated tumors, we have genetically engineered dendritic cells (DC) to express EBV antigens, such as latent me...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.12.10416-10425.1999
更新日期:1999-12-01 00:00:00
abstract::We isolated revertants of a polyomavirus whose origin of DNA replication contains a point mutation in the palindrome to which large T antigen binds. Four independent second-site revertants contain an Asp-286----Asn-286 substitution in large T antigen. This mutant large T antigen activates replication of DNAs containin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.1.242-249.1989
更新日期:1989-01-01 00:00:00
abstract::The herpes simplex virus genome consists of two components, L and S, that invert relative to each other to yield four isomeric arrangements, prototype (P), inversion of the S component (Is), inversion of the L component (Il), and inversion of both components (Isl). Previous studies have shown that the 500-base-pair a ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.58.2.583-591.1986
更新日期:1986-05-01 00:00:00
abstract::Egress of wrapped virus (WV) to the cell periphery following vaccinia virus (VACV) replication is dependent on interactions with the microtubule motor complex kinesin-1 and is mediated by the viral envelope protein A36. Here we report that ectromelia virus (ECTV), a related orthopoxvirus and the causative agent of mou...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06636-11
更新日期:2012-07-01 00:00:00
abstract::A number of positive-strand RNA viruses, such as hepatitis C virus (HCV) and poliovirus, use double-membrane vesicles (DMVs) as replication sites. However, the role of cellular proteins in DMV formation during virus replication is poorly understood. HCV NS4B protein induces the formation of a "membranous web" structur...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00858-19
更新日期:2020-01-06 00:00:00
abstract::Hypoxia-inducible factor 1 (HIF-1) is a ubiquitously expressed transcriptional regulator involved in induction of numerous genes associated with angiogenesis and tumor growth. Kaposi's sarcoma, associated with increased angiogenesis, is a highly vascularized, endothelial cell-derived tumor. Previously, we have shown t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00611-07
更新日期:2007-10-01 00:00:00
abstract::Infection of mice with Moloney murine leukemia virus (M-MuLV) as well as with a nonpathogenic variant, Mo+PyF101 M-MuLV, was studied. Mo+PyF101 M-MuLV differs from wild-type M-MuLV by the addition of enhancer sequences from polyomavirus in the long terminal repeat. Previous experiments indicated that Mo+PyF101 establi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.60.2.423-430.1986
更新日期:1986-11-01 00:00:00
abstract::Pathogens such as HIV-1, with their minimalist genomes, must navigate cellular networks and rely on hijacking and manipulating the host machinery for successful replication. Limited overlap of host factors identified as vital for pathogen replication may be explained by considering that pathogens target, rather than s...
journal_title:Journal of virology
pub_type: 杂志文章,评审
doi:10.1128/JVI.03684-14
更新日期:2015-05-01 00:00:00
abstract::The retrovirus restriction factor TRIM5alpha targets the viral capsid soon after entry. Here we show that the TRIM5alpha protein oligomerizes into trimers. The TRIM5alpha coiled-coil and B30.2(SPRY) domains make important contributions to the formation and/or stability of the trimers. A functionally defective TRIM5alp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.22.14446-14450.2005
更新日期:2005-11-01 00:00:00
abstract::Poliovirus infectious RNA can be synthesized in vitro using phage DNA-dependent RNA-polymerases. These synthetic transcripts contain several extra nucleotides at the 5' end, which are deleted during replication to generate authentic viral genomes. We removed those 5'-end extra nucleotides utilizing a hammerhead ribozy...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.14.6394-6400.2000
更新日期:2000-07-01 00:00:00
abstract::The small envelope protein of hepatitis B virus is the major component of the viral coat and is also secreted from cells as a 20-nm subviral particle, even in the absence of other viral proteins. Such empty envelope particles are composed of approximately 100 copies of this polypeptide and host-derived lipids and are ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.8.4588-4597.1993
更新日期:1993-08-01 00:00:00
abstract::The viral early-to-late switch of papillomavirus infection is tightly linked to keratinocyte differentiation and is mediated in part by alternative mRNA splicing. Here, we report that SRp20, a cellular splicing factor, controls the early-to-late switch via interactions with A/C-rich RNA elements. An A/C-rich SE4 eleme...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01719-08
更新日期:2009-01-01 00:00:00
abstract::The entry of the viral genomic DNA of cauliflower mosaic virus into the nucleus is a critical step of viral infection. We have shown by transient expression in plant protoplasts that the viral coat protein (CP), which is processed from the product of open reading frame IV, contains an N-terminal nuclear localization s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.1.553-560.1999
更新日期:1999-01-01 00:00:00
abstract::The promoter of the murine thymidine kinase gene contains a binding site for transcription factor E2F. Using cell lines (3T3-LT) conditionally expressing polyomavirus large T antigen from a hormone-responsive promoter and reporter gene constructs carrying the thymidine kinase promoter with intact or mutated E2F sites,...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.4.1765-1771.1993
更新日期:1993-04-01 00:00:00