当前位置： SCI文献检索 > JOURNAL OF VIROLOGY期刊下所有文献 > Loss of cytoskeletal transport during egress critically attenuates ectromelia virus infection in vivo.

Loss of cytoskeletal transport during egress critically attenuates ectromelia virus infection in vivo.

Abstract：

:Egress of wrapped virus (WV) to the cell periphery following vaccinia virus (VACV) replication is dependent on interactions with the microtubule motor complex kinesin-1 and is mediated by the viral envelope protein A36. Here we report that ectromelia virus (ECTV), a related orthopoxvirus and the causative agent of mousepox, encodes an A36 homologue (ECTV-Mos-142) that is highly conserved despite a large truncation at the C terminus. Deleting the ECTV A36R gene leads to a reduction in the number of extracellular viruses formed and to a reduced plaque size, consistent with a role in microtubule transport. We also observed a complete loss of virus-associated actin comets, another phenotype dependent on A36 expression during VACV infection. ECTV ΔA36R was severely attenuated when used to infect the normally susceptible BALB/c mouse strain. ECTV ΔA36R replication and spread from the draining lymph nodes to the liver and spleen were significantly reduced in BALB/c mice and in Rag-1-deficient mice, which lack T and B lymphocytes. The dramatic reduction in ECTV ΔA36R titers early during the course of infection was not associated with an augmented immune response. Taken together, these findings demonstrate the critical role that subcellular transport pathways play not only in orthopoxvirus infection in an in vitro context but also during orthopoxvirus pathogenesis in a natural host. Furthermore, despite the attenuation of the mutant virus, we found that infection nonetheless induced protective immunity in mice, suggesting that orthopoxvirus vectors with A36 deletions may be considered another safe vaccine alternative.

journal_name

J Virol

journal_title

Journal of virology

authors

Lynn H,Horsington J,Ter LK,Han S,Chew YL,Diefenbach RJ,Way M,Chaudhri G,Karupiah G,Newsome TP

doi

10.1128/JVI.06636-11

subject

Has Abstract

pub_date

2012-07-01 00:00:00

pages

7427-43

issue

13

eissn

0022-538X

issn

1098-5514

pii

JVI.06636-11

journal_volume

86

pub_type

杂志文章

相关文献

JOURNAL OF VIROLOGY文献大全
  • Unintegrated viral DNA sequences in a hamster tumor induced by bovine papilloma virus.

    abstract：:A fibrosarcoma was induced in a hamster by bovine papilloma virus type 2 (BPV2). The content of BPV2 DNA sequences was measured by DNA-DNA and cRNA-DNA hybridizations. The tumor contained approximately 300 BPV2 genome equivalents per cell. Southern blot hybridization indicated that the viral DNA was in free form, the ...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.39.3.945-949.1981

    authors： Moar MH,Campo MS,Laird HM,Jarrett WF

    更新日期：1981-09-01 00:00:00

  • Human Adenovirus Core Protein V Is Targeted by the Host SUMOylation Machinery To Limit Essential Viral Functions.

    abstract：:Human adenoviruses (HAdV) are nonenveloped viruses containing a linear, double-stranded DNA genome surrounded by an icosahedral capsid. To allow proper viral replication, the genome is imported through the nuclear pore complex associated with viral core proteins. Until now, the role of these incoming virion proteins d...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.01451-17

    authors： Freudenberger N,Meyer T,Groitl P,Dobner T,Schreiner S

    更新日期：2018-01-30 00:00:00

  • Clinical outcome of henipavirus infection in hamsters is determined by the route and dose of infection.

    abstract：:Nipah virus (NiV) and Hendra virus (HeV) are emerging zoonotic viruses and the causative agents of severe respiratory disease and encephalitis in humans. Little is known about the mechanisms that govern the development of respiratory and neurological disease. Using a hamster model of lethal NiV and HeV infection, we d...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.00473-11

    authors： Rockx B,Brining D,Kramer J,Callison J,Ebihara H,Mansfield K,Feldmann H

    更新日期：2011-08-01 00:00:00

  • Fusion formation by the uncleaved spike protein of murine coronavirus JHMV variant cl-2.

    abstract：:The fusogenic properties of the uncleaved spike (S) protein of murine coronavirus JHMV variant cl-2 were studied by expressing the S protein with a deleted putative cleavage site. The amino acid sequence of the putative cleavage site, Arg-Arg-Ala-Arg-Arg, was replaced by Arg-Thr-Ala-Leu-Glu by in vitro mutagenesis of ...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.67.3.1195-1202.1993

    authors： Taguchi F

    更新日期：1993-03-01 00:00:00

  • A C-terminal domain in the avian sarcoma-leukosis virus pol gene product is not essential for viral replication.

    abstract：:The virion proteins encoded by the avian retroviral pol gene (reverse transcriptase and endonuclease) are formed by the proteolytic processing of a gag-pol fusion protein precursor. Recent studies have predicted that the avian sarcoma-leukosis virus pol precursor protein undergoes a previously undetected processing ev...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.62.2.528-533.1988

    authors： Katz RA,Skalka AM

    更新日期：1988-02-01 00:00:00

  • Integrated adenovirus type 12 DNA in the transformed hamster cell line T637: sequence arrangements at the termini of viral DNA and mode of amplification.

    abstract：:Approximately 20 to 22 copies of adenovirus type 12 (Ad12) DNA per cell were integrated into the genome of the cell line T637. Only a few of these copies seemed to remain intact and colinear with virion DNA. All other persisting viral genomes exhibited deletions or inversions or both in the right-hand part of Ad12 DNA...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.42.1.317-321.1982

    authors： Eick D,Doerfler W

    更新日期：1982-04-01 00:00:00

  • Unusual Sp1-GC box interaction in a parvovirus promoter.

    abstract：:The P4 promoter of the parvovirus minute virus of mice contains a single degenerate GC box sequence which binds the transcription factor Sp1 with high affinity. The two promoters of murine Sp1 were affinity purified, and their interactions with the P4 promoter were examined. Several unusual features were observed. Met...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.65.12.6661-6670.1991

    authors： Pitluk ZW,Ward DC

    更新日期：1991-12-01 00:00:00

  • Mapping of the structural gene for the herpes simplex virus type 2 counterpart of herpes simplex virus type 1 glycoprotein C and identification of a type 2 mutant which does not express this glycoprotein.

    abstract：:The gene encoding glycoprotein F (gF) of herpes simplex virus type 2 (HSV-2) was mapped to the region of the viral genome from 0.62 to 0.64 map units. This region is colinear with, and partially homologous to, the region of the HSV-1 genome previously shown to encode gC. Mapping of the gF gene was done by insertion of...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.49.3.741-747.1984

    authors： Zezulak KM,Spear PG

    更新日期：1984-03-01 00:00:00

  • Distinct evolutionary pressures underlie diversity in simian immunodeficiency virus and human immunodeficiency virus lineages.

    abstract：:Simian immunodeficiency virus (SIV) infection of rhesus macaques causes immune depletion and disease closely resembling human AIDS and is well recognized as the most relevant animal model for the human disease. Experimental investigations of viral pathogenesis and vaccine protection primarily involve a limited set of ...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.01862-12

    authors： Fischer W,Apetrei C,Santiago ML,Li Y,Gautam R,Pandrea I,Shaw GM,Hahn BH,Letvin NL,Nabel GJ,Korber BT

    更新日期：2012-12-01 00:00:00

  • The N-terminal region of an entomopoxvirus fusolin is essential for the enhancement of peroral infection, whereas the C-terminal region is eliminated in digestive juice.

    abstract：:The spindles of Anomala cuprea entomopoxvirus (AncuEPV), which are composed of glycoprotein fusolin, are known to enhance the peroral infectivity of AncuEPV itself and of nucleopolyhedroviruses. This has been demonstrated to involve the disruption of intestinal peritrophic membrane (PM), composed of chitin matrix, gly...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.01605-08

    authors： Takemoto Y,Mitsuhashi W,Murakami R,Konishi H,Miyamoto K

    更新日期：2008-12-01 00:00:00

  • Effect of alternating passage on adaptation of sindbis virus to vertebrate and invertebrate cells.

    abstract：:Mosquito-borne alphaviruses, which replicate alternately and obligately in mosquitoes and vertebrates, appear to experience lower rates of evolution than do many RNA viruses that replicate solely in vertebrates. This genetic stability is hypothesized to result from the alternating host cycle, which constrains evolutio...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.79.22.14253-14260.2005

    authors： Greene IP,Wang E,Deardorff ER,Milleron R,Domingo E,Weaver SC

    更新日期：2005-11-01 00:00:00

  • Properties of Corynebacterium acnes bacteriophage and description of an interference phenomenon.

    abstract：:Nine virulent bacteriophages of the anaerobe Corynebacterium (Propionibacterium) acnes, the P-a series, are DNA phages, with long, curved nonretractile tails (130 nm) without tail plates or fibers. They have isometric heads (420 by 460 nm), and are placed in Bradley's group B-1. There is permanent plaque suppression a...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.14.5.1268-1273.1974

    authors： Zierdt CH

    更新日期：1974-11-01 00:00:00

  • Deficiencies in Cellular Processes Modulated by the Retinoblastoma Protein Do Not Account for Reduced Human Cytomegalovirus Replication in Its Absence.

    abstract：UNLABELLED:Despite encoding multiple viral proteins that modulate the retinoblastoma (Rb) protein in a manner classically defined as inactivation, human cytomegalovirus (HCMV) requires the presence of the Rb protein to replicate efficiently. In uninfected cells, Rb controls numerous pathways that the virus also command...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.01718-15

    authors： VanDeusen HR,Kalejta RF

    更新日期：2015-12-01 00:00:00

  • Protein P4 of double-stranded RNA bacteriophage phi 6 is accessible on the nucleocapsid surface: epitope mapping and orientation of the protein.

    abstract：:Protein P4, an early protein of double-stranded RNA bacteriophage phi 6, is a component of the virion-associated RNA polymerase complex and possesses a nucleoside triphosphate (NTP) phosphohydrolase activity. We have produced and characterized a panel of 20 P4-specific monoclonal antibodies. Epitope mapping using trun...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.67.5.2879-2886.1993

    authors： Ojala PM,Juuti JT,Bamford DH

    更新日期：1993-05-01 00:00:00

  • Flock house virus: down-regulation of subgenomic RNA3 synthesis does not involve coat protein and is targeted to synthesis of its positive strand.

    abstract：:Flock house virus is a small insect virus with a bipartite RNA genome consisting of RNA1 and RNA2. RNA3 is a subgenomic element encoded by RNA1, the genomic segment required for viral RNA synthesis (T. M. Gallagher, P. D. Friesen, and R. R. Rueckert, J. Virol. 46:481-489, 1983). Synthesis of RNA3 is strongly inhibited...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.67.5.2716-2722.1993

    authors： Zhong W,Rueckert RR

    更新日期：1993-05-01 00:00:00

  • Bacteriophage T4 virion dihydrofolate reductase: approaches to quantitation and assessment of function.

    abstract：:This paper is concerned with the physiological role(s) of T4 phage-coded dihydrofolate reductase, which functions both in DNA precursor metabolism and as a virion protein. (i) We have detected enzyme activity in noninfectious particles produced under restrictive conditions by gene 11 mutants. This supports the conclus...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.23.3.645-658.1977

    authors： Mosher RA,DiRenzo AB,Mathews CK

    更新日期：1977-09-01 00:00:00

  • Leakage induced in Escherichia coli cells by A protein-RNA complexes from bacteriophage f2.

    abstract：:Complexes of f2 phage RNA and its A protein, or maturation protein, transfect Escherichia coli cells much better than does protein-free RNA. We used these complexes to introduce the bacteriophage f2 lysis gene into cells. The A protein-RNA complex was found to kill cells, probably by causing them to leak large macromo...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.39.1.60-66.1981

    authors： Cody JD,Conway TW

    更新日期：1981-07-01 00:00:00

  • DNA vaccination against Theiler's murine encephalomyelitis virus leads to alterations in demyelinating disease.

    abstract：:Although the etiology of multiple sclerosis (MS) is not known, several factors play a role in this disease: genetic contributions, immunologic elements, and environmental factors. Viruses and virus infections have been associated with the initiation and/or enhancement of exacerbations in MS. Theiler's murine encephalo...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.73.2.993-1000.1999

    authors： Tolley ND,Tsunoda I,Fujinami RS

    更新日期：1999-02-01 00:00:00

  • Purification and characterization of a cellular protein that binds to the downstream activation sequence of the strict late UL38 promoter of herpes simplex virus type 1.

    abstract：:Previous work on the strict late (gamma) UL38 promoter of herpes simplex virus type 1 identified three cis-acting elements required for wild-type levels of transcription: a TATA box at -31, a consensus mammalian initiator element at the transcription start site, and a downstream activation sequence (DAS) at +20 to +33...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.72.10.8181-8190.1998

    authors： Petroski MD,Wagner EK

    更新日期：1998-10-01 00:00:00

  • Identification of an RNA hairpin in poliovirus RNA that serves as the primary template in the in vitro uridylylation of VPg.

    abstract：:The first step in the replication of the plus-stranded poliovirus RNA is the synthesis of a complementary minus strand. This process is initiated by the covalent attachment of UMP to the terminal protein VPg, yielding VPgpU and VPgpUpU. We have previously shown that these products can be made in vitro in a reaction th...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/jvi.74.22.10359-10370.2000

    authors： Paul AV,Rieder E,Kim DW,van Boom JH,Wimmer E

    更新日期：2000-11-01 00:00:00

  • The C-Terminal End of HIV-1 Vpu Has a Clade-Specific Determinant That Antagonizes BST-2 and Facilitates Virion Release.

    abstract：:The cellular protein bone marrow stromal antigen-2 (BST-2)/tetherin acts against a variety of enveloped viruses by restricting their release from the plasma membrane. The HIV-1 accessory protein Vpu counteracts BST-2 by downregulating it from the cell surface and displacing it from virion assembly sites. Previous comp...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.02315-18

    authors： Sharma S,Jafari M,Bangar A,William K,Guatelli J,Lewinski MK

    更新日期：2019-05-15 00:00:00

  • The adenovirus tripartite leader may eliminate the requirement for cap-binding protein complex during translation initiation.

    abstract：:The adenovirus tripartite leader is a 200-nucleotide 5' noncoding region that is found on all late viral mRNAs. This segment is required for preferential translation of viral mRNAs at late times during infection. Most tripartite leader-containing mRNAs appear to exhibit little if any requirement for intact cap-binding...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.62.6.2059-2066.1988

    authors： Dolph PJ,Racaniello V,Villamarin A,Palladino F,Schneider RJ

    更新日期：1988-06-01 00:00:00

  • The pseudorabies virus UL51 gene product is a 30-kilodalton virion component.

    abstract：:Positional homologs to the UL51 open reading frame of herpes simplex virus type 1 have been identified throughout the herpesvirus family. However, no respective protein has so far been described for any of the herpesviruses. With rabbit antisera directed against oligopeptides predicted to comprise antigenic regions of...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.71.7.5635-5638.1997

    authors： Lenk M,Visser N,Mettenleiter TC

    更新日期：1997-07-01 00:00:00

  • The ND10 component promyelocytic leukemia protein relocates to human papillomavirus type 1 E4 intranuclear inclusion bodies in cultured keratinocytes and in warts.

    abstract：:Human papillomavirus type 1 (HPV1) E4 protein is associated with cytoplasmic and nuclear inclusions in productively infected keratinocytes. Here we have used transient expression of HPV1 E4 (also known as E1E4) protein in keratinocytes to reproduce formation of E4 inclusions. Immunofluorescence analysis showed that pr...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/jvi.77.1.673-684.2003

    authors： Roberts S,Hillman ML,Knight GL,Gallimore PH

    更新日期：2003-01-01 00:00:00

  • The human immunodeficiency virus type 1 capsid p2 domain confers sensitivity to the cyclophilin-binding drug SDZ NIM 811.

    abstract：:Human immunodeficiency virus type 1 (HIV-1) specifically incorporates the host cell peptidyl-prolyl isomerase cyclophilin A into virions via contacts with the capsid (CA) domain of the Gag polyprotein Pr55gag. The immunosuppressant drug cyclosporin A and the nonimmunosuppressive cyclosporin A analog SDZ NIM 811 bind t...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.70.9.5751-5757.1996

    authors： Dorfman T,Göttlinger HG

    更新日期：1996-09-01 00:00:00

  • The herpes simplex virus type 1 regulatory protein ICP0 enhances virus replication during acute infection and reactivation from latency.

    abstract：:ICP0 is a potent activator of herpes simplex virus type 1 gene expression in transient assays and in productive infection. A role for ICP0 in reactivation from latency in vivo has also been suggested on the basis of the observation that viruses with mutations in both copies of the diploid gene for ICP0 reactivate less...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.67.12.7501-7512.1993

    authors： Cai W,Astor TL,Liptak LM,Cho C,Coen DM,Schaffer PA

    更新日期：1993-12-01 00:00:00

  • Inhibition of transfer to secondary receptors by heparan sulfate-binding drug or antibody induces noninfectious uptake of human papillomavirus.

    abstract：:Infection with various human papillomaviruses (HPVs) induces cervical cancers. Cell surface heparan sulfates (HS) have been shown to serve as primary attachment receptors, and molecules with structural similarity to cell surface HS, like heparin, function as competitive inhibitors of HPV infection. Here we demonstrate...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.00998-07

    authors： Selinka HC,Florin L,Patel HD,Freitag K,Schmidtke M,Makarov VA,Sapp M

    更新日期：2007-10-01 00:00:00

  • The Kaposi's sarcoma-associated herpesvirus K12 transcript from a primary effusion lymphoma contains complex repeat elements, is spliced, and initiates from a novel promoter.

    abstract：:Kaposi's sarcoma-associated herpesvirus (KSHV) latently infects KS tumors, primary effusion lymphomas (PELs), and PEL cell lines. K12 (T0.7) is the most abundant transcript expressed in latent KSHV infection. We characterize here the K12 transcript from a PEL tumor prior to passage in cell culture. The PEL tumor KSHV ...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/jvi.76.23.11880-11888.2002

    authors： Li H,Komatsu T,Dezube BJ,Kaye KM

    更新日期：2002-12-01 00:00:00

  • Cytoplasmic utilization of human immunodeficiency virus type 1 genomic RNA is not dependent on a nuclear interaction with gag.

    abstract：:In some retroviruses, such as Rous sarcoma virus and prototype foamy virus, Gag proteins are known to shuttle between the nucleus and the cytoplasm and are implicated in nuclear export of the viral genomic unspliced RNA (gRNA) for subsequent encapsidation. A similar function has been proposed for human immunodeficienc...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.06874-11

    authors： Grewe B,Hoffmann B,Ohs I,Blissenbach M,Brandt S,Tippler B,Grunwald T,Uberla K

    更新日期：2012-03-01 00:00:00

  • Antigenic characterization of flavivirus structural proteins separated by isoelectric focusing.

    abstract：:Isoelectrofocusing of nonionic-detergent-disrupted flaviviruses separated the envelope glycoprotein of 53,000 to 58,000 daltons and the nucleocapsid protein of 14,000 daltons. The envelope protein and nucleocapsid protein were isolated at isoelectric points of pI 7.8 and 10.3, respectively. The antigenic determinants ...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.22.3.608-618.1977

    authors： Trent DW

    更新日期：1977-06-01 00:00:00