Abstract:
:The herpes simplex virus genome consists of two components, L and S, that invert relative to each other to yield four isomeric arrangements, prototype (P), inversion of the S component (Is), inversion of the L component (Il), and inversion of both components (Isl). Previous studies have shown that the 500-base-pair a sequences flanking the two components contain a cis-acting site for inversion. In an attempt to insert a third copy of the alpha 4 gene, the major regulatory gene mapping in the repeats flanking the S component, a fragment containing the alpha 4 gene and an origin of DNA synthesis, was recombined into the thymidine kinase gene mapping in the unique sequences of the L component. The resulting recombinants showed massive rearrangements and deletions mapping in the S component and in the junction between the L and S components. One recombinant (R7023) yielded two isomeric DNA arrangements, a major component consisting of Is and a minor component consisting of Isl. In these arrangements, the genome lacked the gene specifying glycoprotein E and all contiguous genes located between it and the alpha 0 gene in the inverted repeats of the L component. Among the deleted sequences were those encoding an origin of viral DNA synthesis, the alpha 47 gene, and the a sequences located at the junction between the L and S-components. The recombinant grew well in rabbit skin, 143TK-, and Vero cell lines. We conclude that the four unique genes deleted in R7023 are not essential for the growth of herpes simplex virus, at least in the cell lines tested, and that the b sequence of the inverted repeats of the L component also contains cis-acting sites for the inversion of herpes simplex virus DNA sequences.
journal_name
J Viroljournal_title
Journal of virologyauthors
Longnecker R,Roizman Bdoi
10.1128/JVI.58.2.583-591.1986subject
Has Abstractpub_date
1986-05-01 00:00:00pages
583-91issue
2eissn
0022-538Xissn
1098-5514journal_volume
58pub_type
杂志文章abstract::Infection by rat virus has been studied in cultures of rat embryo cells to evaluate the Margolis-Kilham hypothesis that the virus preferentially infects tissues with actively dividing cells. An enhancement of infection was seen in cultures infected 10 hr after fresh medium was added as compared to infection of station...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.4.6.872-878.1969
更新日期:1969-12-01 00:00:00
abstract::Due to the biological significance of the carbohydrate component of the human immunodeficiency virus type 1 (HIV-1) glycoproteins in viral pathogenesis, the glycosylation step constitutes an attractive target for anti-HIV therapy. Cyanovirin N (CV-N), which specifically targets the high-mannose (HM) glycans on gp120, ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.12.7777-7784.2005
更新日期:2005-06-01 00:00:00
abstract::Hepatitis A virus (HAV) has previously been reported to bind to human red blood cells through interaction with glycophorin A. Residue K221 of VP1 and the surrounding VP3 residues are involved in such an interaction. This capsid region is specifically recognized by the monoclonal antibody H7C27. A monoclonal antibody-r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00707-12
更新日期:2012-08-01 00:00:00
abstract::Plasmid lambdadv1, which is in a dimeric form, was converted to a linear monomer duplex by the action of EcoRI restriction endonuclease that incises at a unique site in this plasmid genome. The resulting products were then joined by Escherichia coli DNA ligase to produce molecules with various oligomeric forms, and fr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.16.3.479-485.1975
更新日期:1975-09-01 00:00:00
abstract::Human immunodeficiency virus type 2 (HIV-2), like other lentiviruses, is capable of infecting nondividing T cells and macrophages. The present work shows that in HIV-2-infected cells, Vpx is necessary for efficient nuclear import of the preintegration complex. In agreement with this finding, the subcellular localizati...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.13.6162-6167.2000
更新日期:2000-07-01 00:00:00
abstract::Polydnaviridae is a unique family of DNA viruses that are symbiotically associated with parasitoid wasps. Upon oviposition, wasps inject these viruses into their hosts, where they cause several physiological alterations, including suppression of the cellular immune response. Here we report that expression of the glc1....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.3.1861-1870.2005
更新日期:2005-02-01 00:00:00
abstract:UNLABELLED:Human noroviruses (NoVs) cause acute epidemic gastroenteritis. Susceptibility to the majority of NoV infections is determined by genetically controlled secretor-dependent expression of histo-blood group antigens (HBGAs), which are also critical for NoV attachment to host cells. Human NoVs are classified into...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00201-14
更新日期:2014-06-01 00:00:00
abstract::Transfection of an Epstein-Barr virus (EBV)-encoded plasmid containing EBER caused a substantial decrease in the level of plasmid containing EBV in Akata and Mutu Burkitt's lymphoma (BL) lines, but failed to do so in other BL lines. The results suggest that EBER could replace the role of EBV, but other EBV products al...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.20.9977-9982.2001
更新日期:2001-10-01 00:00:00
abstract::Organotypic cultures support the stratification and differentiation of keratinocytes and the human papillomavirus (HPV) life cycle. We report transcription from four novel promoters in the HPV31b upstream regulatory region during the viral life cycle in organotypic cultures. Promoter initiation was not differentiation...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.4.3505-3510.1999
更新日期:1999-04-01 00:00:00
abstract::Vaccinia virus assembles two distinct lipoprotein membranes. The primary membrane contains nonglycosylated proteins, appears as crescents in the cytoplasm, and delimits immature and mature intracellular virions. The secondary or wrapping membrane contains glycoproteins, is derived from virus-modified trans-Golgi or en...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.21.11754-11766.2003
更新日期:2003-11-01 00:00:00
abstract::Rapid transmission of Borna disease virus occurred upon cohabitation of persistently infected and naive rats. Infectious virus, which was abundantly present in fresh urine samples of carrier rats, entered the brains of recipient rats via the olfactory route. Thus, susceptible farm animals possibly acquire the virus fr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.23.12886-12890.2003
更新日期:2003-12-01 00:00:00
abstract::Rubella virus is an enveloped positive-strand RNA virus of the family TOGAVIRIDAE: Virions are composed of three structural proteins: a capsid and two membrane-spanning glycoproteins, E2 and E1. During virus assembly, the capsid interacts with genomic RNA to form nucleocapsids. In the present study, we have investigat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.3.1764-1771.2003
更新日期:2003-02-01 00:00:00
abstract::The complex evolutionary process of human immunodeficiency virus type 1 (HIV-1) is marked by a high level of genetic variation. It has been shown that the HIV-1 genome is characterized by variable and more constant regions, unequal nucleotide frequencies, and preference for G-to-A substitutions. However, this knowledg...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.6.4761-4770.1997
更新日期:1997-06-01 00:00:00
abstract::Human enterovirus 71 (EV-71) is one of the major etiologic causes of hand, foot, and mouth disease (HFMD) among young children worldwide, with fatal instances of neurological complications becoming increasingly common. Global VP1 capsid sequences (n = 628) sampled over 4 decades were collected and subjected to compreh...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01019-09
更新日期:2010-04-01 00:00:00
abstract::African swine fever virus (ASFV) multigene family 360 and 530 (MGF360/530) genes affect viral growth in macrophage cell cultures and virulence in pigs (L. Zsak, Z. Lu, T. G. Burrage, J. G. Neilan, G. F. Kutish, D. M. Moore, and D. L. Rock, J. Virol. 75:3066-3076, 2001). The mechanism by which these novel genes affect ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.4.1858-1864.2004
更新日期:2004-02-01 00:00:00
abstract::ICP0, an alpha (immediate-early) protein of herpes simplex virus 1, performs at least two key functions. It blocks inhibition of viral-gene expression by interferon, a function dependent on the degradation of the ND10 components PML and SP100 by the ubiquitin ligase expressed by the RING finger (RF), and it blocks sil...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01940-08
更新日期:2009-01-01 00:00:00
abstract::The copy choice model for the generation of defective interfering (DI) particles of vesicular stomatitis virus suggests that during replication the polymerase prematurely terminates, moves with the nascent daughter strand to another site on the same or a different template molecule, and resumes elongation of the nasce...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.51.2.515-521.1984
更新日期:1984-08-01 00:00:00
abstract::The coding capacity of human cytomegalovirus (HCMV) for glycoproteins by far exceeds that of other herpesviruses. Few of these proteins have been characterized so far. We have investigated the gene product of reading frame UL132. The putative protein product of UL132 is a glycoprotein with a theoretical mass of 29.8 k...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.18.11837-11847.2005
更新日期:2005-09-01 00:00:00
abstract::Human coronaviruses (HCoV) are respiratory pathogens with neuroinvasive, neurotropic, and neurovirulent properties, highlighting the importance of studying the potential implication of these viruses in neurological diseases. The OC43 strain (HCoV-OC43) was reported to induce neuronal cell death, which may participate ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01513-16
更新日期:2016-12-16 00:00:00
abstract::Recognition of viruses by germ line-encoded pattern recognition receptors of the innate immune system is essential for rapid production of type I interferon (IFN) and early antiviral defense. We investigated the mechanisms of viral recognition governing production of type I IFN during herpes simplex virus (HSV) infect...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01167-07
更新日期:2007-12-01 00:00:00
abstract::The amount and distribution of viral DNA were established in a horse acutely infected with the Wyoming strain of equine infectious anemia virus (EIAV). The highest concentration of viral DNA were found in the liver, lymph nodes, bone marrow, and spleen. The kidney, choroid plexus, and peripheral blood leukocytes also ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.12.5194-5200.1989
更新日期:1989-12-01 00:00:00
abstract::The nucleic acids of influenza A, B, and C viruses were compared. Susceptibility to nucleases demonstrates that influenza C virus, just as influenza A and B viruses, possesses single-stranded RNA as its genome. The base compositions of the RNAs of influenza A, B, and influenza C virus are almost identical and comparat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.18.2.738-744.1976
更新日期:1976-05-01 00:00:00
abstract::Rotavirus is the major cause of diarrhea among young infants in both humans and animals. Immune protection of newborns by vaccination is difficult to achieve since there is not enough time to mount an immune response before exposure to the virus. We have designed a vaccination strategy mediating transfer of neutralizi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.19.8966-8971.2000
更新日期:2000-10-01 00:00:00
abstract::Hepatitis E virus (HEV), a positive-strand RNA virus, is an important causative agent of waterborne hepatitis. Expression of cDNA (encoding amino acids 1 to 979 of HEV nonstructural open reading frame 1) in insect cells resulted in synthesis of a 110-kDa protein (P110), a fraction of which was proteolytically processe...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.14.6249-6255.2001
更新日期:2001-07-01 00:00:00
abstract::Escherichia coli K-12 wild type and a uvrA mutant derivative were used to construct isogenic strains bearing one, two, three, or more phage lambda cI genomes and containing increasing concentration of lambda repressor as measured by in vitro operator DNA-binding assays. The survival and phage induction in response to ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.26.3.595-602.1978
更新日期:1978-06-01 00:00:00
abstract::The mechanism of lysis by in vivo-induced cytotoxic T lymphocytes (CTL) was examined with virus-specific CTL from mice infected with lymphocytic choriomeningitis virus (LCMV). LCMV-induced T cells were shown to have greater than 10 times the serine esterase activity of T cells from normal mice, and high levels of seri...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.8.3726-3733.1990
更新日期:1990-08-01 00:00:00
abstract::Monoclonal antibodies (MAbs) were raised against human immunodeficiency virus type 1 gp120. One MAb, P4/D10, was found to mediate highly efficient antibody-dependent cellular cytotoxicity and virus neutralization. The reactivity was located to a major neutralizing region (amino acids 304 to 323) on gp120. Five other M...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.2.936-940.1990
更新日期:1990-02-01 00:00:00
abstract:UNLABELLED:Due to a scarcity of immunocompetent animal models for viral hepatitis, little is known about the early innate immune responses in the liver. In various hepatotoxic models, both pro- and anti-inflammatory activities of recruited monocytes have been described. In this study, we compared the effect of liver in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03733-14
更新日期:2015-05-01 00:00:00
abstract::Vaccinia virus has a wide host range and infects mammalian cells of many different species. This suggests that the cell surface receptors for vaccinia virus are ubiquitously expressed and highly conserved. Alternatively, different receptors are used for vaccinia virus infection of different cell types. Here we report ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.2.1577-1585.1998
更新日期:1998-02-01 00:00:00
abstract::Extracellular murine leukemia virus (MLV) reverse transcriptase activity was decreased by interferon treatment in four interferon-sensitive mouse cell lines which were chronic MLV producers. In three cell lines which were relatively insensitive to interferon, extracellular enzyme activity remained unchanged by interfe...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.16.3.569-574.1975
更新日期:1975-09-01 00:00:00