Abstract:
:Hepatitis delta virus (HDV) replicates its genome in the nucleus of an infected cell. However, an unsolved problem has been the identification in the cytoplasm of a putative mRNA for the synthesis of the only virus-coded protein, the delta antigen. We now report the characterization of an 800-base RNA that is cytoplasmic, polyadenylated, and antigenomic and that should direct the translation of the delta antigen. This RNA was about 500 times less abundant than full-length genomic RNA. We mapped the predominant 5' terminus and also the 3' site at which the poly(A) is added. At a point 15 to 20 bases upstream of the poly(A) addition site is the sequence AAUAAA, which could have been used as a signal for the polyadenylation. When an infectious cDNA clone of the whole HDV genome was changed at this site to UUUAAA, the clone was no longer infectious and it was unable to direct the synthesis of the delta antigen. These findings provided additional evidence that the polyadenylated RNA was at least the predominant method for the expression of the delta antigen. Apparently the HDV RNA was processed as if it were a host mRNA polymerase II transcript, although this did not necessarily indicate that HDV RNA was transcribed with this enzyme.
journal_name
J Viroljournal_title
Journal of virologyauthors
Hsieh SY,Chao M,Coates L,Taylor Jdoi
10.1128/JVI.64.7.3192-3198.1990subject
Has Abstractpub_date
1990-07-01 00:00:00pages
3192-8issue
7eissn
0022-538Xissn
1098-5514journal_volume
64pub_type
杂志文章abstract::The Epstein-Barr virus (EBV) immediate-early protein BZLF1 mediates the switch between the latent and lytic forms of EBV infection and has been previously shown to induce a G(1)/S block in cell cycle progression in some cell types. To examine the effect of BZLF1 on cellular gene expression, we performed microarray ana...
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.7.2321-2328.1988
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pub_type: 杂志文章
doi:10.1128/JVI.67.9.5299-5302.1993
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.59.2.494-499.1986
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journal_title:Journal of virology
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1993-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.3.2450-2459.1999
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
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pub_type: 杂志文章
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pub_type: 临床试验,杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2014-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1970-09-01 00:00:00
abstract::An unusual set of 3' coterminal, spliced mRNAs transcribed through the BamHI A fragment have been previously identified in nasopharyngeal carcinoma (NPC) tissues. These RNAs have also been detected at low levels in Burkitt's lymphoma (BL) cell lines and Epstein-Barr virus (EBV)-transformed lymphocytes. Sequence analys...
journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1997-04-01 00:00:00