Alanine scanning of the hepatitis C virus core protein reveals numerous residues essential for production of infectious virus.

abstract：:Reovirus encephalitis in mice was used as a model system to investigate astrocyte activation (astrogliosis) following viral infection of the brain. Reovirus infection resulted in astrogliosis, as evidenced by increased expression of glial fibrillary acidic protein (GFAP), and the upregulation of genes that have been p...

journal_title：Journal of virology

authors： Clarke P,Zhuang Y,Berens HM,Leser JS,Tyler KL

更新日期：2019-05-01 00:00:00

abstract：:The phenotypic defects of two mutants of vaccinia virus, the lesions of which map to gene G2R, were characterized in vivo, and the results suggest a role for the G2R protein in viral transcription elongation. Both a temperature-sensitive mutant, Cts56, and an isatin-beta-thiosemicarbazone-dependent deletion mutant, G2...

journal_title：Journal of virology

authors： Black EP,Condit RC

更新日期：1996-01-01 00:00:00

abstract：:Cauliflower mosaic virus (CaMV) forms two types of inclusion bodies within infected plant cells: numerous virus factories, which are the sites for viral replication and virion assembly, and a single transmission body (TB), which is specialized for virus transmission by aphid vectors. The TB reacts within seconds to ap...

journal_title：Journal of virology

authors： Bak A,Gargani D,Macia JL,Malouvet E,Vernerey MS,Blanc S,Drucker M

更新日期：2013-11-01 00:00:00

abstract：:Moloney murine leukemia virus (M-MuLV) and M-MuLV-derived retroviral vectors are not expressed in early mouse embryos or in embryonal carcinoma cells. M-MuLV-derived mutants or M-MuLV-related variants which transduce the neomycin phosphotransferase gene can, however, induce drug resistance in embryonal carcinoma cells...

journal_title：Journal of virology

authors： Weiher H,Barklis E,Ostertag W,Jaenisch R

更新日期：1987-09-01 00:00:00

abstract：:Mouse mammary tumor virus (MMTV) DNA fragments were cloned into M13 bacteriophage, and the single-stranded recombinant phage DNAs were used as strand-specific nucleic acid hybridization probes to measure synthesis of plus (genomic) and minus strands of MMTV RNA in cultured cell lines and in cell-free preparations of n...

journal_title：Journal of virology

authors： Prewitt RS,Washington LD,Stallcup MR

更新日期：1984-04-01 00:00:00

abstract：:Hepatitis C virus (HCV) is a major causative agent of posttransfusion non-A, non-B hepatitis, which often develops into malignant chronic diseases, including liver cirrhosis and hepatocellular carcinoma. We have cloned from human carriers overlapping cDNAs (9,416 bp) covering the entire coding region of the HCV genome...

journal_title：Journal of virology

authors： Takamizawa A,Mori C,Fuke I,Manabe S,Murakami S,Fujita J,Onishi E,Andoh T,Yoshida I,Okayama H

更新日期：1991-03-01 00:00:00

abstract：:Human cytomegalovirus (HCMV) has evolved multiple strategies for suppression of the antiviral response of the infected cell. DNA array technology has revealed that HCMV clearly regulates host gene expression during the course of a productive infection by enhancing, sustaining, or suppressing steady-state levels of cel...

journal_title：Journal of virology

authors： Gealy C,Denson M,Humphreys C,McSharry B,Wilkinson G,Caswell R

更新日期：2005-01-01 00:00:00

abstract：:Spumaviruses, or foamy viruses, express Gag proteins that are incompletely processed by the viral protease in cell cultures. To delineate the proteolytic cleavage sites between potential Gag subdomains, recombinant human spumaretrovirus (HSRV) Gag proteins of different lengths were expressed, purified by affinity chro...

journal_title：Journal of virology

authors： Pfrepper KI,Löchelt M,Rackwitz HR,Schnölzer M,Heid H,Flügel RM

更新日期：1999-09-01 00:00:00

abstract：:The emerging porcine epidemic diarrhea virus (PEDV) requires trypsin supplementation to activate its S protein for membrane fusion and virus propagation in cell culture. By substitution of a single amino acid in the S protein, we created a recombinant PEDV with an artificial furin protease cleavage site N terminal of ...

journal_title：Journal of virology

authors： Li W,Wicht O,van Kuppeveld FJ,He Q,Rottier PJ,Bosch BJ

更新日期：2015-08-01 00:00:00

abstract：:The genome of the Snyder-Theilen strain of feline sarcoma virus (ST-FeSV) is a 4.3-kilobase-pair (kbp) RNA molecule that contains a 1.5-kbp cellular insertion (fes gene) flanked by feline leukemia virus sequences at its 5' end (1.6 kbp) and 3' end (1.2 kbp) (Sherr et al., J. Virol. 34:200-212, 1980). DNA transfection ...

journal_title：Journal of virology

authors： Barbacid M

更新日期：1981-01-01 00:00:00

abstract：:Monoclonal antibody vpg15 detects a 24-kDa cell surface protein on feline cells permissive for infection with feline immunodeficiency virus (FIV). The antibody blocks infection of FIV-susceptible cells, and expression of the vpg15 marker is decreased in FIV-infected cells in vitro. These results suggest that the antib...

journal_title：Journal of virology

authors： Hosie MJ,Willett BJ,Dunsford TH,Jarrett O,Neil JC

更新日期：1993-03-01 00:00:00

abstract：:Cas-Br-M is an ecotropic murine leukemia virus (MuLV) of wild-mouse origin that causes neurogenic hind-limb paralysis. By virtue of its N-tropism, the virus replicates well in tissues of mice bearing the n but not the b allele at the Fv-1 locus. To determine if different Fv-1n strains of mice were equally susceptible ...

journal_title：Journal of virology

authors： Hoffman PM,Morse HC 3rd

更新日期：1985-01-01 00:00:00

abstract：:For paramyxoviruses, entry requires a receptor-binding protein (hemagglutinin-neuraminidase [HN], H, or G) and a fusion protein (F). Like other class I viral fusion proteins, F is expressed as a prefusion metastable protein that undergoes a refolding event to induce fusion. HN binding to its receptor triggers F refold...

journal_title：Journal of virology

authors： Connolly SA,Leser GP,Jardetzky TS,Lamb RA

更新日期：2009-11-01 00:00:00

abstract：:Influenza A viruses (IAVs) are viral pathogens that cause epidemics and occasional pandemics of significant mortality. The generation of efficacious vaccines and antiviral drugs remains a challenge due to the rapid appearance of new influenza virus types and antigenic variants. Consequently, novel strategies for the p...

journal_title：Journal of virology

authors： Jiang M,Österlund P,Westenius V,Guo D,Poranen MM,Bamford DH,Julkunen I

更新日期：2019-02-05 00:00:00

abstract：:The mechanism by which murine polyomavirus penetrates cells and arrives at the nucleus, the site of viral replication, is not well understood. Simian virus 40 and JC virus, two closely related members of the polyomavirus subfamily, use caveola- and clathrin-mediated uptake pathways for entry, respectively. The data pr...

journal_title：Journal of virology

authors： Gilbert JM,Benjamin TL

更新日期：2000-09-01 00:00:00

abstract：UNLABELLED:The DNA damage response (DDR) that evolved to repair host cell DNA damage also recognizes viral DNA entering the nucleus during infections. Here, we investigated the modulation of DDR signaling during de novo infection of primary endothelial cells by Kaposi's sarcoma-associated herpesvirus (KSHV). Phosphoryl...

journal_title：Journal of virology

authors： Singh VV,Dutta D,Ansari MA,Dutta S,Chandran B

更新日期：2014-03-01 00:00:00

abstract：:NSP5 (NS26), the product of rotavirus gene 11, is a phosphoprotein whose role in the virus replication cycle is unknown. To gain further insight into its function, we obtained monoclonal antibodies against the baculovirus-expressed protein. By immunoprecipitation and immunoblotting experiments, we showed that (i) NSP5...

journal_title：Journal of virology

authors： Poncet D,Lindenbaum P,L'Haridon R,Cohen J

更新日期：1997-01-01 00:00:00

abstract：:Numerous RNA viruses generate subgenomic mRNAs (sgRNAs) for expression of their 3'-proximal genes. A major step in control of viral gene expression is the regulation of sgRNA synthesis by specific promoter elements. We used barley yellow dwarf virus (BYDV) as a model system to study transcriptional control in a virus ...

journal_title：Journal of virology

authors： Koev G,Miller WA

更新日期：2000-07-01 00:00:00

abstract：:The recently developed subgenomic hepatitis C virus (HCV) replicons were limited by the fact that the sequence encoding the structural proteins was missing. Therefore, important information about a possible influence of these proteins on replication and pathogenesis and about the mechanism of virus formation could not...

journal_title：Journal of virology

authors： Pietschmann T,Lohmann V,Kaul A,Krieger N,Rinck G,Rutter G,Strand D,Bartenschlager R

更新日期：2002-04-01 00:00:00

abstract：:Dengue virus (DV) infection can cause either a self-limiting flu-like disease or a threatening hemorrhage that may evolve to shock and death. A variety of cell types, such as dendritic cells, monocytes, and B cells, can be infected by DV. However, despite the role of T lymphocytes in the control of DV replication, the...

journal_title：Journal of virology

authors： Silveira GF,Wowk PF,Cataneo AHD,Dos Santos PF,Delgobo M,Stimamiglio MA,Lo Sarzi M,Thomazelli APFS,Conchon-Costa I,Pavanelli WR,Antonelli LRV,Báfica A,Mansur DS,Dos Santos CND,Bordignon J

更新日期：2018-04-27 00:00:00

abstract：:Two human papillomavirus type 16 (HPV 16)-immortalized human keratinocyte cell lines (HPK) were shown to have retained the ability for differentiation after subcutaneous injection into nude mice. These properties were maintained even at late passage. HPK cells gave rise to transiently growing cysts which exhibited an ...

journal_title：Journal of virology

authors： Dürst M,Bosch FX,Glitz D,Schneider A,zur Hausen H

更新日期：1991-02-01 00:00:00

abstract：:Measles virus (MeV), like all viruses of the order Mononegavirales, utilizes a complex consisting of genomic RNA, nucleoprotein, the RNA-dependent RNA polymerase, and a polymerase cofactor, the phosphoprotein (P), for transcription and replication. We previously showed that a recombinant MeV that does not express anot...

journal_title：Journal of virology

authors： Pfaller CK,Bloyet LM,Donohue RC,Huff AL,Bartemes WP,Yousaf I,Urzua E,Clavière M,Zachary M,de Masson d'Autume V,Carson S,Schieferecke AJ,Meyer AJ,Gerlier D,Cattaneo R

更新日期：2020-01-31 00:00:00

abstract：:Vaccinia virus has a wide host range and infects mammalian cells of many different species. This suggests that the cell surface receptors for vaccinia virus are ubiquitously expressed and highly conserved. Alternatively, different receptors are used for vaccinia virus infection of different cell types. Here we report ...

journal_title：Journal of virology

authors： Chung CS,Hsiao JC,Chang YS,Chang W

更新日期：1998-02-01 00:00:00

abstract：:The current therapy for hepatitis B and C is based on systemic administration of recombinant human alpha interferon (r-hIFN-alpha). However, systemic delivery of r-hIFN-alpha is associated with severe side effects, but more importantly, it is effective in only a small percentage of patients. In an effort to maximize I...

journal_title：Journal of virology

authors： Aurisicchio L,Delmastro P,Salucci V,Paz OG,Rovere P,Ciliberto G,La Monica N,Palombo F

更新日期：2000-05-01 00:00:00

abstract：:Bovine papillomavirus type 1 replication was previously shown to require both the E1 initiator protein and the E2 transactivator protein. We show here that E1, in the absence of E2, is sufficient for low-level bovine papillomavirus type 1 DNA replication in C-33A cells. In addition, studies of genetically isolated E2 ...

journal_title：Journal of virology

authors： Grossel MJ,Sverdrup F,Breiding DE,Androphy EJ

更新日期：1996-10-01 00:00:00

abstract：:Kaposi's sarcoma-associated herpesvirus (KSHV) (also called human herpesvirus 8) is consistently found in Kaposi's sarcoma lesions and in body-cavity-based lymphomas. A 17-kb KSHV lambda clone was obtained directly from a Kaposi's sarcoma lesion. DNA sequence analysis of this clone identified an open reading frame whi...

journal_title：Journal of virology

authors： Li M,Lee H,Yoon DW,Albrecht JC,Fleckenstein B,Neipel F,Jung JU

更新日期：1997-03-01 00:00:00

abstract：:We report that adaptation to infect the guinea pig did not modify the capacity of foot-and-mouth disease virus (FMDV) to kill suckling mice and to cause an acute and transmissible disease in the pig, an important natural host for this pathogen. Adaptive amino acid replacements (I(248)-->T in 2C, Q(44)-->R in 3A, and L...

journal_title：Journal of virology

authors： Núñez JI,Molina N,Baranowski E,Domingo E,Clark S,Burman A,Berryman S,Jackson T,Sobrino F

更新日期：2007-08-01 00:00:00

abstract：:Gene expression of nonsegmented negative-sense RNA viruses involves sequential synthesis of monocistronic mRNAs and transcriptional attenuation at gene borders resulting in a transcript gradient. To address the role of the heterogeneous rabies virus (RV) intergenic regions (IGRs) in transcription attenuation, we const...

journal_title：Journal of virology

authors： Finke S,Cox JH,Conzelmann KK

更新日期：2000-08-01 00:00:00

abstract：:N-terminal modifications of the chemokine RANTES bind to C-C chemokine receptor 5 (CCR5) and block human immunodeficiency virus type 1 (HIV-1) infection with greater efficacy than native RANTES. Modified RANTES compounds induce rapid CCR5 internalization and much slower receptor reexpression than native RANTES, sugges...

journal_title：Journal of virology

authors： Sabbe R,Picchio GR,Pastore C,Chaloin O,Hartley O,Offord R,Mosier DE

更新日期：2001-01-01 00:00:00

abstract：:A hallmark of infection with herpes simplex virus type 1 (HSV-1) is the establishment of latency in ganglia of the infected individual. During the life of the latently infected individual, the virus can occasionally reactivate, travel back to the eye, and cause recurrent disease. Indeed, a major cause of corneal scarr...

journal_title：Journal of virology

authors： Mott KR,Bresee CJ,Allen SJ,BenMohamed L,Wechsler SL,Ghiasi H

更新日期：2009-03-01 00:00:00