Abstract:
:The mechanism by which murine polyomavirus penetrates cells and arrives at the nucleus, the site of viral replication, is not well understood. Simian virus 40 and JC virus, two closely related members of the polyomavirus subfamily, use caveola- and clathrin-mediated uptake pathways for entry, respectively. The data presented here indicate that compounds that block endocytosis of both caveola- and clathrin-derived vesicles have no effect on polyomavirus infectivity. Polyomavirus does not appear to colocalize with either clathrin light chain or caveolin-1 by immunofluorescence microscopy. Additionally, expression of a dominant-negative form of dynamin I has no effect on polyomavirus uptake and infectivity. Therefore, polyomavirus uptake occurs through a class of uncoated vesicles in a clathrin-, caveolin-1-, and dynamin I-independent manner.
journal_name
J Viroljournal_title
Journal of virologyauthors
Gilbert JM,Benjamin TLdoi
10.1128/jvi.74.18.8582-8588.2000subject
Has Abstractpub_date
2000-09-01 00:00:00pages
8582-8issue
18eissn
0022-538Xissn
1098-5514journal_volume
74pub_type
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