Abstract:
:A covalently closed circular form of unintegrated viral DNA obtained from NIH 3T3 cells freshly infected with Moloney murine leukemia virus (M-MLV) and a port of the endogenous M-MLV from the BALB/Mo mouse strain have been cloned in bacteriophage lambda. The unintegrated viral DNA was cleaved with restriction endonuclease HindIII and inserted into the single HindIII site of lambda phage Charon 21A. Similarly high-molecular-weight DNA from BALB/Mo mice ws cleaved sequentially with restriction endonucleases EcoRI and HindIII and separated on the basis of size, and one of the two fractions which reacted with an M-MLV-specific complementary DNA was inserted into the HindIII site of Charon 21A. Recombinant clones containing M-MLV-reacting DNA were analyzed by restriction endonuclease mapping, heteroduplexing, and infectivity assays. The restriction endonuclease map of the insert derived from unintegrated viral DNA, lambda x MLV-1, was comparable to published maps. Electron microscope analysis of the hybrid formed between lambda x MLV-1 DNA and 35S genomic M-MLV RNA showed a duplex structure. The molecularly cloned lambda x MLV-1 DNA contained only one copy of the long terminal repeat and was not infectious even after end-to-end ligation of the insert DNA. The insert DNA derived from endogenous M-MLV, lambda x MLVint-1, contained a DNA stretch measuring 5.4 kilobase pairs in length, corresponding to the 5' part of the genomic viral RNA, and cellular mouse DNA sequences measuring 3.5 kilobase pairs in length. The viral part of the insert showed the typical restriction pattern of M-MLV DNA except that a single restriction site, PvuII, in the 5' long terminal repeat was missing. Reconstructed genomes containing the 5' half derived from the integrated viral DNA and the 3' half derived from the unintegrated viral DNA were able to induce XC plaques after transfection in uninfected mouse fibroblasts.
journal_name
J Viroljournal_title
Journal of virologyauthors
Berns AJ,Lai MH,Bosselman RA,McKennett MA,Bacheler LT,Fan H,Maandag EC,van der Putten HV,Verma IMdoi
10.1128/JVI.36.1.254-263.1980subject
Has Abstractpub_date
1980-10-01 00:00:00pages
254-63issue
1eissn
0022-538Xissn
1098-5514journal_volume
36pub_type
杂志文章abstract::To investigate E7-dependent biochemical changes which are involved in cellular transformation, we analyzed the influence of human papillomavirus type 16 (HPV-16) E7 on the expression of cell cycle regulatory proteins. Expression of E7 in established rodent fibroblasts (NIH 3T3), which was shown to be sufficient for tr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.10.6389-6399.1995
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abstract::Mutants of bacteriophage T4D that are defective in genes 42 (dCMP hydroxymethylase), 46 (DNA exonuclease), and 56 (dCTPase) produce limited amounts of phage DNA in Escherichia coli B. In this DNA, glucoylated 5-hydroxymethylcytosine is completely replaced by cytosine. We found that this DNA rapidly becomes fragmented ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.48.1.18-30.1983
更新日期:1983-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.30.3.711-719.1979
更新日期:1979-06-01 00:00:00
abstract::Mutants of polyomavirus with altered host specificities were isolated in undifferentiated C2 myoblast cells (L. Ricci, R. Maione, C. Passananti, A. Felsani, and P. Amati, J. Virol. 66:7153-7158, 1992). The mutations responsible for this phenotype belonged to either of the two classes: a large duplication of the enhanc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.2.1196-1199.1994
更新日期:1994-02-01 00:00:00
abstract::ICP0, one of the five immediate-early (IE) gene products of herpes simplex virus (HSV), is a potent activator of transcription. To assess the biological activities of ICP0 and to explore its mechanisms of action, two helper-independent recombinant adenoviruses were constructed. In each recombinant, the E1 region was s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.12.4544-4553.1988
更新日期:1988-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01044-08
更新日期:2008-09-01 00:00:00
abstract::We have previously shown that delivery of the porcine type I interferon gene (poIFN-α/β) with a replication-defective human adenovirus vector (adenovirus 5 [Ad5]) can sterilely protect swine challenged with foot-and-mouth disease virus (FMDV) 1 day later. However, the need of relatively high doses of Ad5 limits the ap...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03462-12
更新日期:2013-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.34.3.764-771.1980
更新日期:1980-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.78.22.12529-12536.2004
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.5.1593-1601.1987
更新日期:1987-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.24.2.478-488.1977
更新日期:1977-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.11.4913-4918.1989
更新日期:1989-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01419-10
更新日期:2010-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.11.5242-5249.2000
更新日期:2000-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.79.6.3254-3266.2005
更新日期:2005-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01306-20
更新日期:2020-09-15 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.3.2450-2459.1999
更新日期:1999-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.22.1.175-186.1977
更新日期:1977-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.12.10020-10028.1998
更新日期:1998-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.5.3247-3250.1992
更新日期:1992-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01783-12
更新日期:2012-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.4.2896-2904.1998
更新日期:1998-04-01 00:00:00
abstract::A fresh inoculum of human adenovirus type 12 (Ad12) was obtained from the American Type Culture Collection and passaged once on human embryonic kidney cells, and Ad12 DNA was prepared from the first-passage yield to avoid higher passages which might have generated host-virus DNA recombinants. The 18 PstI fragments of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.1.379-389.1994
更新日期:1994-01-01 00:00:00
abstract::We have previously shown that the nonconserved carboxy-terminal exon of the adenovirus type 2 E1A-289R protein contains two interchangeable sequence elements, auxiliary region (AR) 1 and AR2, that are required for efficient CR3-mediated transcriptional activation of the viral E4 promoter (M. Bondesson, C. Svensson, S....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.7.5978-5983.1998
更新日期:1998-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.10.2.318-319.1972
更新日期:1972-08-01 00:00:00
abstract::Sonchus yellow net virus is a plant nucleorhabdovirus whose nucleocapsid (N), phosphoprotein (P), and polymerase (L) proteins form large viroplasms in the nuclei of infected plants (C. R. F. Martins, J. A. Johnson, D. M. Lawrence, T. J. Choi, A. Pisi, S. L. Tobin, D. Lapidus, J. D. O. Wagner, S. Ruzin, K. McDonald, an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02349-06
更新日期:2007-05-01 00:00:00
abstract::The mammalian APOBEC3 proteins are an important component of the cellular innate immune response to retroviral infection. APOBEC3G can extinguish HIV-1 infectivity by its incorporation into virus particles and subsequent cytosine deaminase activity that attacks the nascent viral cDNA during reverse transcription, caus...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00056-10
更新日期:2010-07-01 00:00:00
abstract::From purified linear and superhelical DNAs, the restriction endonuclease maps of four xenotropic murine leukemia virus DNAs from NFS, NZB, BALB/c, and AKR mice were determined with ten restriction endonucleases. Each xenotropic proviral DNA was found to be a unique restriction endonuclease map, with differences in the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.40.3.963-970.1981
更新日期:1981-12-01 00:00:00
abstract::Most eukaryotic initiation factors (eIFs) are required for internal translation initiation at the internal ribosome entry site (IRES) of picornaviruses. eIF4B is incorporated into ribosomal 48S initiation complexes with the IRES RNA of foot-and-mouth disease virus (FMDV). In contrast to the weak interaction of eIF4B w...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.9.7505-7514.1999
更新日期:1999-09-01 00:00:00
abstract::We evaluated the ability of human coronaviruses to infect primary cultures of human neural cells. Double immunofluorescence with antibodies to virus and cell markers showed infection of fetal astrocytes and of adult microglia and astrocytes by strain OC43. RNA amplification revealed infection of fetal astrocytes, adul...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.1.800-806.1997
更新日期:1997-01-01 00:00:00