Nontemplated terminal nucleotidyltransferase activity of double-stranded RNA bacteriophage phi6 RNA-dependent RNA polymerase.

abstract：:Disease-associated PrP fragments produced upon in vitro or in vivo proteolysis can provide significant insight into the causal strain of prion disease. Here we describe a novel molecular strain typing assay that used thermolysin digestion of caudal medulla samples to produce PrPres signatures on Western blots that rea...

journal_title：Journal of virology

authors： Owen JP,Rees HC,Maddison BC,Terry LA,Thorne L,Jackman R,Whitelam GC,Gough KC

更新日期：2007-10-01 00:00:00

abstract：:The Gag proteins of retroviruses are the only viral products required for the release of membrane-enclosed particles by budding from the host cell. Particles released when these proteins are expressed alone are identical to authentic virions in their rates of budding, proteolytic processing, and core morphology, as we...

journal_title：Journal of virology

authors： Krishna NK,Campbell S,Vogt VM,Wills JW

更新日期：1998-01-01 00:00:00

abstract：:Human papillomaviruses (HPVs) infect keratinocytes of skin and mucosa. Homeostasis of these constantly renewing, stratified epithelia is maintained by balanced keratinocyte proliferation and terminal differentiation. Instructions from the extracellular matrix engaging integrins strongly regulate these keratinocyte fun...

journal_title：Journal of virology

authors： Oldak M,Smola H,Aumailley M,Rivero F,Pfister H,Smola-Hess S

更新日期：2004-10-01 00:00:00

abstract：:Cas-Br-M is an ecotropic murine leukemia virus (MuLV) of wild-mouse origin that causes neurogenic hind-limb paralysis. By virtue of its N-tropism, the virus replicates well in tissues of mice bearing the n but not the b allele at the Fv-1 locus. To determine if different Fv-1n strains of mice were equally susceptible ...

journal_title：Journal of virology

authors： Hoffman PM,Morse HC 3rd

更新日期：1985-01-01 00:00:00

abstract：:The Vpr protein, encoded by the human immunodeficiency virus type 1 (HIV-1) genome, is one of the nonstructural proteins packaged in large amounts into viral particles. We have previously reported that Vpr associates with the DNA repair enzyme uracil DNA glycosylase (UDG). In this study, we extended these observations...

journal_title：Journal of virology

authors： Willetts KE,Rey F,Agostini I,Navarro JM,Baudat Y,Vigne R,Sire J

更新日期：1999-02-01 00:00:00

abstract：:Viruses efficiently block the host antiviral response in order to replicate and spread before host intervention. The mechanism initiating antiviral immunity during stealth viral replication is unknown, but recent data demonstrate that defective viral genomes generated at peak virus replication are critical for this pr...

journal_title：Journal of virology

authors： López CB

更新日期：2014-08-01 00:00:00

abstract：:Immune activation is a major characteristic of human immunodeficiency virus type 1 (HIV-1) infection and a strong prognostic factor for HIV-1 disease progression. The underlying mechanisms leading to immune activation in viremic HIV-1 infection, however, are not fully understood. Here we show that, following the initi...

journal_title：Journal of virology

authors： Meier A,Alter G,Frahm N,Sidhu H,Li B,Bagchi A,Teigen N,Streeck H,Stellbrink HJ,Hellman J,van Lunzen J,Altfeld M

更新日期：2007-08-01 00:00:00

abstract：:We isolated and characterized two spontaneous, weakly leukemogenic mutants of Rauscher spleen focus-forming virus (R-SFFV) that contain mutations in nonoverlapping regions of the membrane envelope (env) glycoprotein gene. As reported previously (M. Ruta and D. Kabat, J. Virol. 35:844-853, 1980), the replication-defect...

journal_title：Journal of virology

authors： Machida CA,Bestwick RK,Kabat D

更新日期：1984-02-01 00:00:00

abstract：:The therapeutic efficacy of direct drug infusion into the brain, the target organ of transmissible spongiform encephalopathies, was assessed in transgenic mice intracerebrally infected with 263K scrapie agent. Pentosan polysulfate (PPS) gave the most dramatic prolongation of the incubation period, and amphotericin B h...

journal_title：Journal of virology

authors： Doh-ura K,Ishikawa K,Murakami-Kubo I,Sasaki K,Mohri S,Race R,Iwaki T

更新日期：2004-05-01 00:00:00

abstract：:Positional homologs to the UL51 open reading frame of herpes simplex virus type 1 have been identified throughout the herpesvirus family. However, no respective protein has so far been described for any of the herpesviruses. With rabbit antisera directed against oligopeptides predicted to comprise antigenic regions of...

journal_title：Journal of virology

authors： Lenk M,Visser N,Mettenleiter TC

更新日期：1997-07-01 00:00:00

abstract：:Monoclonal antibodies (MAbs) directed against the E2 glycoprotein of mouse hepatitis virus (MHV) have been classified according to their ability to bind to either of the two purified 90,000-molecular-weight subunits (90K subunits) of the 180K peplomeric glycoprotein E2. Correlation with previously reported information...

journal_title：Journal of virology

authors： Weismiller DG,Sturman LS,Buchmeier MJ,Fleming JO,Holmes KV

更新日期：1990-06-01 00:00:00

abstract：:Poliovirus inactivation by ammonia causes a slight reduction in the sedimentation coefficients of viral particles, but has no detectable effect on either the electrophoretic pattern of viral capsid proteins or the isoelectric points of inactivated particles. These virions still attach to cells, but are unable to repre...

journal_title：Journal of virology

authors： Ward RL

更新日期：1978-05-01 00:00:00

abstract：:The hepatitis B virus (HBV) X protein (HBx) is a multifunctional regulator of cellular signal transduction and transcription pathways and has a critical role in HBV replication. Much of the cytoplasmic signal transduction activity associated with HBx expression and its stimulation of viral replication is attributable ...

journal_title：Journal of virology

authors： Bouchard MJ,Wang L,Schneider RJ

更新日期：2006-05-01 00:00:00

abstract：:A lysate from hypotonically swollen polyoma-infected BALB/3T3 cells incorporated labeled deoxynucleotide triphosphates into both viral and cellular DNAs. The incorporation was stimulated by the presence of ATP, deoxynucleotide triphosphates, thiols, and magnesium ions. Strong inhibition of incorporation was observed w...

journal_title：Journal of virology

authors： Hunter T,Francke B

更新日期：1974-01-01 00:00:00

abstract：:Vaccinia virus (VACV) stimulates long-term immunity against highly pathogenic orthopoxvirus infection of humans (smallpox) and mice (mousepox [ectromelia virus {ECTV}]) despite the lack of a natural host-pathogen relationship with either of these species. Previous research revealed that VACV is able to induce polyfunc...

journal_title：Journal of virology

authors： Hersperger AR,Siciliano NA,Eisenlohr LC

更新日期：2012-07-01 00:00:00

abstract：:Moloney murine leukemia virus (M-MuLV) IN-IN protein interactions important for catalysis of strand transfer and unimolecular and bimolecular disintegration reactions were investigated by using a panel of chemically modified M-MuLV IN proteins. Functional complementation of an HHCC-deleted protein (Ndelta105) by an in...

journal_title：Journal of virology

authors： Donzella GA,Leon O,Roth MJ

更新日期：1998-02-01 00:00:00

abstract：:Avian influenza virus (AIV) surveillance in Antarctica during 2013 revealed the prevalence of evolutionarily distinct influenza viruses of the H11N2 subtype in Adélie penguins. Here we present results from the continued surveillance of AIV on the Antarctic Peninsula during 2014 and 2015. In addition to the continued d...

journal_title：Journal of virology

authors： Hurt AC,Su YCF,Aban M,Peck H,Lau H,Baas C,Deng YM,Spirason N,Ellström P,Hernandez J,Olsen B,Barr IG,Vijaykrishna D,Gonzalez-Acuna D

更新日期：2016-10-14 00:00:00

abstract：:Q beta phage RNAs with inactivating insertion (8-base) or deletion (17-base) mutations within their replicase genes were prepared from modified Q beta cDNAs and transfected into Escherichia coli spheroplasts containing Q beta replicase provided in trans by a resident plasmid. Replicase-defective (Rep-) Q beta phage pr...

journal_title：Journal of virology

authors： Palasingam K,Shaklee PN

更新日期：1992-04-01 00:00:00

abstract：:Immune responses and the components of protective immunity following norovirus infection in humans are poorly understood. Although antibody responses following norovirus infection have been partially characterized, T cell responses in humans remain largely undefined. In contrast, T cells have been shown to be essentia...

journal_title：Journal of virology

authors： LoBue AD,Lindesmith LC,Baric RS

更新日期：2010-09-01 00:00:00

abstract：:The herpes simplex virus 1 infected cell protein 22 (ICP22), the product of the alpha22 gene, is a nucleotidylylated and phosphorylated nuclear protein with properties of a transcriptional factor required for the expression of a subset of viral genes. Here, we report the following. (i) ICP22 interacts with a previousl...

journal_title：Journal of virology

authors： Bruni R,Roizman B

更新日期：1998-11-01 00:00:00

abstract：:The glycopeptides obtained by pronase digestion of two ecotropic strains of murine leukemia virus (MuLV) were compared by gel filtration. Four different glycopeptide size classes, designated G(1), G(2), G(3), and G(4), with molecular weights of approximately 5,100, 2,900, 2,200, and 1,500, respectively, were shown to ...

journal_title：Journal of virology

authors： Kemp MC,Basak S,Compans RW

更新日期：1979-07-01 00:00:00

abstract：:In this study, we show that human cytomegalovirus DNA synthesis is inhibited in infected confluent human embryonic lung cells treated with the DNA-intercalative topoisomerase II inhibitor 4-9'-(acridinylamino)methanesulfon-m-anisidide (m-AMSA). Similar inhibitory effects were observed with VM-26, a nonintercalative to...

journal_title：Journal of virology

authors： Benson JD,Huang ES

更新日期：1988-12-01 00:00:00

abstract：:The role of the 5' nontranslated region in the replication of hepatitis A virus (HAV) was studied by analyzing the translation and replication of chimeric RNAs containing the encephalomyocarditis virus (EMCV) internal ribosome entry segment (IRES) and various lengths (237, 151, or 98 nucleotides [nt]) of the 5'-termin...

journal_title：Journal of virology

authors： Jia XY,Tesar M,Summers DF,Ehrenfeld E

更新日期：1996-05-01 00:00:00

abstract：UNLABELLED:Respiratory syncytial virus (RSV), a member of the Paramyxoviridae family of nonsegmented, negative-sense, single-stranded RNA genome viruses, is a leading cause of lower respiratory tract infections in infants, young children, and the elderly or immunocompromised. There are many open questions regarding the...

journal_title：Journal of virology

authors： Kiss G,Holl JM,Williams GM,Alonas E,Vanover D,Lifland AW,Gudheti M,Guerrero-Ferreira RC,Nair V,Yi H,Graham BS,Santangelo PJ,Wright ER

更新日期：2014-07-01 00:00:00

abstract：:The Middle East respiratory syndrome coronavirus (MERS-CoV) nonstructural protein 16 (nsp16) is an S-adenosyl-l-methionine (SAM)-dependent 2'-O-methyltransferase (2'-O-MTase) that is thought to methylate the ribose 2'-OH of the first transcribed nucleotide (N1) of viral RNA cap structures. This 2'-O-MTase activity is ...

journal_title：Journal of virology

authors： Aouadi W,Blanjoie A,Vasseur JJ,Debart F,Canard B,Decroly E

更新日期：2017-02-14 00:00:00

abstract：:The cloned transcription factor hepatocyte nuclear factor 1 (HNF1) transactivates transcription from the hepatitis B virus (HBV) large surface antigen promoter but does not influence the transcriptional activities of the other three HBV promoters. This indicates that this transcription factor can differentially influe...

journal_title：Journal of virology

authors： Raney AK,Easton AJ,Milich DR,McLachlan A

更新日期：1991-11-01 00:00:00

abstract：:Tumor necrosis factor alpha (TNF-alpha) induced morphologic changes such as chromatin condensation and cell shrinkage in a feline fibroblastic cell line (CRFK) chronically infected with feline immunodeficiency virus (FIV) but not in uninfected CRFK cells. DNA extracted from TNF-alpha-treated CRFK cells infected with F...

journal_title：Journal of virology

authors： Ohno K,Nakano T,Matsumoto Y,Watari T,Goitsuka R,Nakayama H,Tsujimoto H,Hasegawa A

更新日期：1993-05-01 00:00:00

abstract：:The nodavirus flock house virus (FHV) has a bipartite, positive-sense, RNA genome that encodes the catalytic subunit of the RNA replicase and the viral capsid protein precursor on separate genomic segments (RNA1 and RNA2, respectively). RNA1 can replicate autonomously when transfected into permissive cells, allowing s...

journal_title：Journal of virology

authors： Johnson KL,Ball LA

更新日期：1999-10-01 00:00:00

abstract：:HA20, a mutant of Bacillus subtilis phage SPO1, is deficient in both DNA replication and late transcription. HA20 contains mutations in two different genes, which suggested that the two effects might be caused independently. However, single-mutation derivatives, affected only in gene 27, were deficient for both activi...

journal_title：Journal of virology

authors： Stewart CR

更新日期：1984-01-01 00:00:00

abstract：:RNA-dependent protein kinase PKR is an important regulator of gene expression in interferon (IFN)-treated and virus-infected cells. The 50-kb gene encoding human PKR kinase (pkr) is inducible by IFN. Transfection analyses, using chloramphenicol acetyltransferase (CAT) as the reporter in constructs possessing various 5...

journal_title：Journal of virology

authors： Kuhen KL,Vessey JW,Samuel CE

更新日期：1998-12-01 00:00:00