A point mutation abolishes the helicase but not the nucleoside triphosphatase activity of hepatitis C virus NS3 protein.

Abstract:

:The NS3 protein of hepatitis C virus contains a bipartite structure consisting of an N-terminal serine protease and a C-terminal DEAD box helicase. We show that the C-terminal domain has ATPase and panhelicase activities. The integrity of the helicase function is dependent on the conserved DEAD motif and can be abolished by a His-Ala point mutation, leaving a fully functional nucleoside triphosphatase.

journal_name

J Virol

journal_title

Journal of virology

authors

Heilek GM,Peterson MG

doi

10.1128/JVI.71.8.6264-6266.1997

subject

Has Abstract

pub_date

1997-08-01 00:00:00

pages

6264-6

issue

8

eissn

0022-538X

issn

1098-5514

journal_volume

71

pub_type

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