Abstract:
:The covalent linkage of oncornavirus-specific DNA to chicken DNA was investigated in normal chicken embryo fibroblasts (CEF) and in virus-producing leukemic cells transformed by avian myeloblastosis virus (AMV). The virus-specific sequences present in cellular DNA fractionated by different methods were detected by DNA-RNA hybridization by using 70S AMV RNA as a probe. In CEF and in leukemic cells, the viral DNA appeared to be present only in the nucleus. After cesium chloride-ethidium bromide density equilibrium sedimentation, the viral DNA was present as linear, double-stranded molecules not separable from linear chicken DNA. After extraction by the Hirt procedure, the viral DNA precipitated with the high-molecular-weight DNA. After alkaline sucrose velocity sedimentation, the viral DNA cosedimented with the high-molecular-weight cellular DNA. The results indicate that in both types of cells studied, the oncornavirus-specific DNA sequences were linked by alkali stable bonds to nuclear cellular DNA of high molecular weight and did not appear to be present in free form of any size.
journal_name
J Viroljournal_title
Journal of virologyauthors
Markham PD,Baluda MAdoi
10.1128/JVI.12.4.721-732.1973subject
Has Abstractpub_date
1973-10-01 00:00:00pages
721-32issue
4eissn
0022-538Xissn
1098-5514journal_volume
12pub_type
杂志文章abstract::It has been shown previously in the severe acute respiratory syndrome coronavirus (SARS-CoV) that two point mutations, N15A and V25F, in the transmembrane domain (TMD) of the envelope (E) protein abolished channel activity and led to in vivo attenuation. Pathogenicity was recovered in mutants that also regained E prot...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02158-16
更新日期:2017-02-14 00:00:00
abstract::Abnormal prion protein (PrP(Sc)) plays a central role in the transmission of prion diseases, but the molecular basis of prion strains with distinct biological characteristics remains to be elucidated. We analyzed the characteristics of prion disease by using mice inoculated with the Chandler and Fukuoka-1 strains prop...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.11.7104-7112.2005
更新日期:2005-06-01 00:00:00
abstract::The effect of bacteriophage SPO1 infection of Bacillus subtilis and a deoxyribonucleic acid (DNA) polymerase-deficient (pol(-)) mutant of this microorganism on the synthesis of DNA has been examined. Soon after infection, the incorporation of deoxyribonucleoside triphosphates into acid-insoluble material by cell lysat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.9.2.263-272.1972
更新日期:1972-02-01 00:00:00
abstract::West Nile virus (WNV) replicates in the skin; however, cell targets in the skin have not been identified. In the current studies, WNV infected the epidermis and adnexal glands of mouse skin, and the epidermal cells were identified as keratinocytes by double labeling for WNV antigen and keratin 10. Inoculation of mice ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02692-10
更新日期:2011-05-01 00:00:00
abstract::The genome of influenza A virus consists of eight single-strand negative-sense RNA segments, each comprised of a coding region and a noncoding region. The noncoding region of the NS segment is thought to provide the signal for packaging; however, we recently showed that the coding regions located at both ends of the h...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.6.3766-3774.2005
更新日期:2005-03-01 00:00:00
abstract::The rate of transcription in isolated nuclei of primary mouse kidney cells increases by a factor of about 1.5 between 5 and 8 h after infection with polyoma virus or simian virus 40. This process requires intact virus and is inhibited by cycloheximide and actinomycin D. It appears to involve the activity of all three ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.35.1.8-19.1980
更新日期:1980-07-01 00:00:00
abstract::Newborn mice suckled on dams immunized either orally or parenterally with primate rotavirus SA-11 were protected against diarrhea induced by SA-11 virus challenge. Experimental oral administration of milk from orally immunized dams protected suckling mice against challenge; protective activity was detected both in the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.54.1.58-64.1985
更新日期:1985-04-01 00:00:00
abstract::The Epstein-Barr virus nuclear antigen 2 (EBNA-2) acidic domain is essential for B-lymphocyte growth transformation and can activate transcription when brought to a promoter by a sequence-specific DNA-binding domain. We now show that the EBNA-2 acidic domain has slightly less activity than the proteotypic acidic trans...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.1.585-588.1995
更新日期:1995-01-01 00:00:00
abstract::Viral recombination has been postulated to play two roles in the development of human immunodeficiency virus (HIV) resistance to antiretroviral drugs. First, recombination has the capacity to associate resistance mutations expressed by distinct viruses, thereby contributing to the development of viruses with improved ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00083-07
更新日期:2007-07-01 00:00:00
abstract::The herpes simplex virus 2 (HSV-2) viral microRNA (miRNA) designated miR-H6 is located upstream of the latency-associated transcript (LAT) promoter region on the strand opposite the LAT. Deletion of the LAT promoter and part of LAT exon 1 abolished HSV-2 miR-H6 expression in acutely and latently infected guinea pig do...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01997-10
更新日期:2011-05-01 00:00:00
abstract::The replication proteins Rep and Rep' of porcine circovirus type 1 (PCV1) are both capable of introducing and resealing strand discontinuities at the viral origin of DNA replication in vitro underlying genome amplification by rolling-circle replication. The PCV1 origin of replication encompasses the minimal binding si...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02420-06
更新日期:2007-06-01 00:00:00
abstract::Theiler's virus-induced demyelinating disease results from a chronic infection in the white matter of the central nervous system and provides an excellent model for human multiple sclerosis. Like multiple sclerosis, there are genetic risk factors in disease development, including genes associated with the major histoc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.2.686-690.1990
更新日期:1990-02-01 00:00:00
abstract::Extrachromosomal DNA was isolated from tissue culture cells that were acutely infected with simian sarcoma virus (SSV) and its associated helper (simian sarcoma-associated virus [SSAV]). Two sizes of closed circular viral genomic DNA intermediates were isolated, cleaved at the single EcoRI site, and ligated to the Cha...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.41.2.593-604.1982
更新日期:1982-02-01 00:00:00
abstract::We tested whether the selection of target sites can be manipulated by fusing retroviral integrase with a sequence-specific DNA-binding protein. A hybrid protein that has the Escherichia coli LexA protein fused to the C terminus of the human immunodeficiency virus type 1 integrase was constructed. The fusion protein, I...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.1.37-46.1996
更新日期:1996-01-01 00:00:00
abstract::Ribosomes are observed intimately associated with nucleocapsids of vesicular, stomatitis virus, especially those that line structures that are either cytoplasmic vesicles or invaginations of the plasma membrane. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.16.2.447-452.1975
更新日期:1975-08-01 00:00:00
abstract::Naked DNA vaccines expressing the prM and E genes of two tick-borne flaviviruses, Russian spring summer encephalitis (RSSE) virus and Central European encephalitis (CEE) virus were evaluated in mice. The vaccines were administered by particle bombardment of DNA-coated gold beads by Accell gene gun inoculation. Two imm...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.12.9563-9569.1997
更新日期:1997-12-01 00:00:00
abstract::The plus strand of virion DNA of duck hepatitis B virus possessed, at its 5' terminus, a capped oligoribonucleotide 18 to 19 bases in length. This oligoribonucleotide had a unique 5' end, the heterogeneity in length reflecting two distinct junctions with plus-strand DNA that were 1 base apart. The sequence of the RNA ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.57.1.229-236.1986
更新日期:1986-01-01 00:00:00
abstract::The mechanism of action of hepatitis B virus (HBV) X protein in transcriptional transactivation and in tumorigenesis remains obscure. We have used the yeast two-hybrid system to identify a cellular protein that can interact with HBV X protein. This protein, designated X-associated protein 1 (XAP-1), is a human homolog...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.2.1107-1114.1995
更新日期:1995-02-01 00:00:00
abstract::Chloramphenicol affects several steps in the DNA replication of mycoplasma virus L51, a noncytocidal, naked, bullet-shaped virion containing circular single-stranded (SS) DNA of 1.5 X 10(6) daltons (4.5 kilobases). In the presence of chloramphenicol, adsorption was normal and parental SS DNA was converted to double-st...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.44.3.877-881.1982
更新日期:1982-12-01 00:00:00
abstract::Many replication events are involved in the influenza A virus life cycle, and they are accomplished by different virus proteins with specific functions. However, because the size of the influenza virus genome is limited, the virus uses different mechanisms to express multiple viral proteins from a single gene segment....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02656-12
更新日期:2013-03-01 00:00:00
abstract::A herpes simplex virus type 1 variant [C239(TK-)] harboring a deletion in the thymidine kinase (TK) gene was assessed for capacity to establish latent infections. Outbred Swiss Webster mice were inoculated on both hind footpads, and numbers of neurons expressing latency-associated transcript and amounts of viral DNA i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.11.4976-4978.1989
更新日期:1989-11-01 00:00:00
abstract::Alpha/beta interferons (IFN-alpha/beta) are key mediators of innate immunity and important modulators of adaptive immunity. The mechanisms by which IFN-alpha/beta are induced are becoming increasingly well understood. Recent studies showed that Toll-like receptors 7 and 8 expressed by plasmacytoid dendritic cells (pDC...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.16.10376-10385.2005
更新日期:2005-08-01 00:00:00
abstract::We have found that the replicative helicase E1 of bovine papillomavirus type 1 (BPV-1) interacts with a key cell cycle regulator of S phase, the cyclin E-Cdk2 kinase. The E1 helicase, which interacts with cyclin E and not with Cdk2, presents the highest affinity for catalytically active kinase complexes. In addition, ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.9.7255-7262.1998
更新日期:1998-09-01 00:00:00
abstract::Noroviruses (NoVs) are a leading cause of gastroenteritis worldwide, yet host factors that restrict NoV replication are not well understood. Here, we use a CRISPR activation genome-wide screening to identify host genes that can inhibit murine norovirus (MNoV) replication in human cells. Our screens identified with hig...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01324-18
更新日期:2018-12-10 00:00:00
abstract::The level of RNase activity in human interferon preparations was examined. Although sequential purification of interferon resulted in nearly a 300-fold increase in specific activity, RNase-specific activity remained more or less constant. The implications of this finding for the analyses of the mode of action of inter...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.21.3.1247-1248.1977
更新日期:1977-03-01 00:00:00
abstract::The sheep genome harbors approximately 20 endogenous retroviruses (enJSRVs) highly related to the exogenous Jaagsiekte sheep retrovirus (JSRV). One of the enJSRV loci, enJS56A1, acts as a unique restriction factor by blocking JSRV in a transdominant fashion at a late stage of the retroviral cycle. To better understand...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01859-06
更新日期:2007-02-01 00:00:00
abstract::Influenza A viruses of the H9N2 subtype are endemic in poultry in many Eurasian countries and have occasionally caused clinical respiratory diseases in humans. While some avian H9N2 viruses have glutamine (Q) at amino acid position 226 of the hemagglutinin (HA) receptor-binding site, an increasing number of isolates h...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02827-06
更新日期:2007-05-01 00:00:00
abstract::Human papillomaviruses (HPVs) infect keratinocytes of skin and mucosa. Homeostasis of these constantly renewing, stratified epithelia is maintained by balanced keratinocyte proliferation and terminal differentiation. Instructions from the extracellular matrix engaging integrins strongly regulate these keratinocyte fun...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.19.10738-10746.2004
更新日期:2004-10-01 00:00:00
abstract::An immunochemical analysis of the hemagglutinin (VP4) of the simian rotavirus SA11 was performed to better understand the structure and function of this molecule. Following immunization of mice with double-shelled virus particles and VP4-enriched fractions from CsCl gradients, a battery of anti-SA11 hybridomas was gen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.6.2164-2172.1988
更新日期:1988-06-01 00:00:00
abstract::Enterovirus 71 (EV71) (genus Enterovirus, family Picornaviridae), a common cause of hand, foot, and mouth disease (HFMD), may also cause severe neurological diseases, such as encephalitis and poliomyelitis-like paralysis. To examine the genetic diversity and rate of evolution of EV71, we have determined and analyzed c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.12.9969-9975.1999
更新日期:1999-12-01 00:00:00